Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,731,621 G→T M845I (ATG→ATT)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,731,6210GT97.8% 135.3 / NA 46M845I (ATG→ATT) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (22/23);  minor base C (1/0);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

TCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATC  >  NC_000913_3_pae_pgi/1731477‑1731761
                                                                                                                                                |                                                                                                                                            
tCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCAACGGAACGTTTGATTCGTc                                                                                                                                          >  1:262920/1‑149 (MQ=255)
      cccGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGa                                                                                                                                    >  2:890109/1‑149 (MQ=255)
       ccGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAAAAAAAAAAAGAAAAATATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGaa                                                                                                                                   <  2:796585/149‑1 (MQ=255)
            ttGGCAACAACGGCTGCGCGCCAGTTAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTg                                                                                                                              <  2:27515/149‑1 (MQ=255)
                        gCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                  <  2:989704/149‑1 (MQ=255)
                               gcCAGTCAGTTGCTGGAACTCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGa                                                                                                           <  1:13916/149‑1 (MQ=255)
                               gcCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCGAATATCCGa                                                                                                           >  2:326066/1‑149 (MQ=255)
                                ccAGGCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCGTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                                                                          <  1:454274/149‑1 (MQ=255)
                                    tcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAAGCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAGTATCCGATGTaa                                                                                                      >  1:801087/1‑149 (MQ=255)
                                     caggTGCTGGAACTCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAGCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAc                                                                                                     <  1:6448/149‑1 (MQ=255)
                                              gAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAc                                                                                            >  1:1178721/1‑149 (MQ=255)
                                                     cTCAGGGGTATGCGGATTTTCCGGTCAGTCGGGGAACCCTACGCGGATGTTTGCAAGAGGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCGGAACGGTTGCGAAATTCGAATATCCGATGTAACGACCACTGCGCCTTCg                                                                                     <  2:846457/149‑1 (MQ=255)
                                                      tCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGc                                                                                    >  2:1125432/1‑149 (MQ=255)
                                                                  ggATTTTCCGATCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGACCGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCACATATCCGATGTAACGACCCCTACGCCCCCCCCTTTCGcaccca                                                                        >  1:1177561/1‑144 (MQ=255)
                                                                   gATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAg                                                                       >  1:427414/1‑149 (MQ=255)
                                                                       ttCCGGTAATTCTCGAACCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGACGCCTGAACGGTGGCGAAATGCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa                                                                   <  2:1163990/149‑1 (MQ=255)
                                                                         ccGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAtt                                                                 >  1:829760/1‑149 (MQ=255)
                                                                             tCATGCTCGAAACCCTACGCGAATTTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc                                                             <  2:187586/149‑1 (MQ=255)
                                                                               aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAACTTCAAATATCCGGTGTACCGACCGCTACACCGTCGCCTTTCGCCACGAGCTTAATGTTCgg                                                           >  1:146045/1‑149 (MQ=255)
                                                                               aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCgg                                                           <  2:909731/149‑1 (MQ=255)
                                                                                 tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAc                                                                                                  <  1:353472/108‑1 (MQ=255)
                                                                                 tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAc                                                                                                  >  2:353472/1‑108 (MQ=255)
                                                                                      caaCCCTACGCGAATATCTGCAACATGTTTATGATCGTCCCGAGCTGGAGCGTTTGATTCGGCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGCCCGTTGTGCCCTCGTCTTTCGCCACAAGTTTATTGTTCGGCTATGTc                                                    <  2:317539/148‑1 (MQ=255)
                                                                                        aCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTcgc                                                  <  1:1125432/149‑1 (MQ=255)
                                                                                           cTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGa                                               >  2:928690/1‑149 (MQ=255)
                                                                                            tACGTGAATGTTTGCAAGATGTTTATGATCTTCCCGCATTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGaa                                              <  1:245547/149‑1 (MQ=255)
                                                                                                 gAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCGATACTTTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTAt                                         <  1:890109/149‑1 (MQ=255)
                                                                                                    tGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTCTGTCGCGGAATTTATGTa                                      >  1:133894/1‑149 (MQ=255)
                                                                                                         gCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCaga                                 <  2:1178721/149‑1 (MQ=255)
                                                                                                            aGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCg                              >  1:266149/1‑149 (MQ=255)
                                                                                                                   tATGATCTTCCCGCACTGGCACGTTTGCTCCGTCGCCTGACCGGTGCCGAAATTCAAATATCAGATGAAGCAACCACTACGCCCCCCCCTTTCGCCACGAGTACATCGTTCGACTGTGTCGCGGAACTTATGTACACGAGCGcccccca                       >  2:761355/1‑148 (MQ=255)
                                                                                                                    aTGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCg                      >  1:172417/1‑149 (MQ=255)
                                                                                                                     tGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGc                     >  2:997451/1‑149 (MQ=255)
                                                                                                                      gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCt                    >  1:289649/1‑149 (MQ=255)
                                                                                                                      gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCCCt                    >  2:260629/1‑149 (MQ=255)
                                                                                                                            cccGCACCGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCaga              <  1:611542/149‑1 (MQ=255)
                                                                                                                             gcgaaCTGGAACGGTTGATTCGGCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCagag             <  2:146045/145‑1 (MQ=255)
                                                                                                                              cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGaa                                              <  2:27326/115‑1 (MQ=255)
                                                                                                                              cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGaa                                              >  1:27326/1‑115 (MQ=255)
                                                                                                                                cACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTGTGTAGCAGAGCGACGCCCCGCTGGCGAAgcga          >  1:91138/1‑148 (MQ=255)
                                                                                                                                 aCTGGAACGTTTGATTTGTCGGCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGcc         <  1:326066/149‑1 (MQ=255)
                                                                                                                                   tGGAACTTTGGATTCGTCGCCTGAACGGTGGTGTAATTCAAATATCCGATGTAACGACCACCACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCTGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCgg       <  2:863419/149‑1 (MQ=255)
                                                                                                                                   tGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCgg       <  2:1078948/149‑1 (MQ=255)
                                                                                                                                   tGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCgg       >  2:1005291/1‑149 (MQ=255)
                                                                                                                                     gAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCACCGCTGGCAGAGCGCCGGGc     >  2:644850/1‑149 (MQ=255)
                                                                                                                                        cGTTTGATTCGTCGCCTGAACGGTGGCAAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATc  <  1:669451/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
TCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATC  >  NC_000913_3_pae_pgi/1731477‑1731761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑