Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	1,731,621	G→T	M845I (ATG→ATT)	lhr →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	1,731,621	0	G	T	100.0%	63.3 / NA	20	M845I (ATG→ATT)	lhr	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/10);  total (10/10)

TCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCG  >  NC_000913_3_pae_pgi/1731477‑1731763                                                                                                                                                 |                                                                                                                                               tCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAAGGTTTGATTCGTc                                                                                                                                            >  1:61696/1‑149 (MQ=255)   gCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                          <  1:27966/149‑1 (MQ=255)         cGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAGTGTCTGCAAGATTTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAAc                                                                                                                                    <  1:74017/149‑1 (MQ=255)                        ggCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCAGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaa                                                                                                                     <  2:488963/149‑1 (MQ=255)                              gcgcCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATcc                                                                                                               >  2:292176/1‑149 (MQ=255)                                gcCAGTCAGTTGCTGGGAATCGCTCAGGGGTATCCGGATTTTCCGGTCAGTCTCGAAGCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGa                                                                                                             <  1:283870/149‑1 (MQ=255)                                               gAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAc                                                                                              <  2:339652/149‑1 (MQ=255)                                                                 ccGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGGTTGATTCGTCGCCTGAACGGGGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCcac                                                                            >  1:266215/1‑149 (MQ=255)                                                                   ggATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACaa                                                                          <  1:127280/149‑1 (MQ=255)                                                                               cATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAAAGTTTGATTCGTCGCCTGCACGGTGGCGACATTCACATATCCTATGTAACGACCACTACGCCCTCGGCAGTCGCCCCAGCTTTCTTGTTCg                                                              >  2:85488/1‑149 (MQ=255)                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCgg                                                             <  1:292993/149‑1 (MQ=255)                                                                                   ctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTa                                                          >  2:492230/1‑149 (MQ=255)                                                                                      gAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa                                                                     <  1:433256/135‑1 (MQ=255)                                                                                      gAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa                                                                     >  2:433256/1‑135 (MQ=255)                                                                                                     tGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTa                                        <  1:492230/149‑1 (MQ=255)                                                                                                              gATGTTGATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCGTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGa                               <  1:201021/149‑1 (MQ=255)                                                                                                                               cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTAGTTACCAGAGCGACGCCCCGCTGGCagacc              >  2:195636/1‑147 (MQ=255)                                                                                                                                    tGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGCTTATTGTTCGGCTATGTCGCGGAATTTCTGTACCAGAGCGACGCCCCGCTGGCACAGCGCCgg         >  1:493087/1‑149 (MQ=255)                                                                                                                                       aaCGTTTGATTCGTCGCCTGCACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTATTGTACCAGCGCGACGGGCCGCTGGCAGAGCCGCGGGCa      >  1:467795/1‑149 (MQ=255)                                                                                                                                           tttGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCg  >  2:13098/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                               TCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCG  >  NC_000913_3_pae_pgi/1731477‑1731763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑