BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	3,214,770	A→C	E575A (GAA→GCA)	rpoD →	RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	3,214,770	0	A	C	100.0%	351.0 / NA	104	E575A (GAA→GCA)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (56/48); total (56/48)

Only 100 of 108 total aligned reads displayed.

CACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTA  >  NC_000913_3_pae_pgi/3214624‑3214911

caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGaagcag                                                                                                                                             >  1:986484/1‑149 (MQ=255)

caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGATGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGaagcag                                                                                                                                             >  2:982888/1‑149 (MQ=255)

caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCCTGCGGCAACGCACGACGTGCTGTCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATAGATATGACCACCGACTCCCCGCTTGcagaag                                                                                                                                             >  2:310107/1‑149 (MQ=255)

 accCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGt                                                                                                                                            <  1:1323385/149‑1 (MQ=255)

 accCTCGAGCTGCCGCTAGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGt                                                                                                                                            >  1:148783/1‑149 (MQ=255)

   ccccGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTgg                                                                                                                                          <  1:1306323/146‑1 (MQ=255)

   ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTCCACGCTGGAAGCAGTgg                                                                                                                                          >  1:194190/1‑149 (MQ=255)

   ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTgg                                                                                                                                          >  1:198599/1‑149 (MQ=255)

      cGATCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGAGATGAACACCGACTACACGCTGGAAGCAGTGGGTa                                                                                                                                       <  2:837911/149‑1 (MQ=255)

          cTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACa                                                                                                                                   <  1:598229/149‑1 (MQ=255)

              cGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTc                                                                                                                               >  1:17782/1‑149 (MQ=255)

              cGCTGGATTAGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTc                                                                                                                               <  1:689488/149‑1 (MQ=255)

               gCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACGACACGCTGGAAGCAGTGGGTAAACAGTTCg                                                                                                                              >  1:476448/1‑149 (MQ=255)

                cTGGATTCTGCGACCACCGAAAGCGTGCTTGCGGCAACGCACGACGTGCTGGCTGGCCTGAACGCGCGGGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGACCACCGACTACACGCAGGAAGCAGTGGGTAAACCGGTCGa                                                                                                                             >  1:1115085/1‑149 (MQ=255)

                 tGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGAc                                                                                                                            <  1:436583/149‑1 (MQ=255)

                   gATTCTGCGACCACCGAAAGCCTGCGTGCGGCGACGCACGACGTGCAGGCTTGCCTGACCGCGCGAGAACCAAAAGTTCGGCGTATGAGTTTCGGTATCGATATGAACACCAACTACACGCTGGAAGCGGGGGTTAAACAGTGCGAccg                                                                                                                          >  2:685211/1‑147 (MQ=255)

                   gATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGt                                                                                                                          >  2:35453/1‑149 (MQ=255)

                    aTTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGtt                                                                                                                         >  1:1262840/1‑149 (MQ=255)

                     ttCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTCAACACTGTAAGCGCTGGGTAAACAGTTCGACGTTa                                                                                                                        >  2:1061517/1‑149 (MQ=255)

                     ttCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGAc                                                                                                                            <  2:596411/145‑1 (MQ=255)

                     ttCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGAc                                                                                                                            >  1:596411/1‑145 (MQ=255)

                           gACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCcgcg                                                                                                                  >  2:1028274/1‑149 (MQ=255)

                           gACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCcgcg                                                                                                                  >  2:1310430/1‑149 (MQ=255)

                           gACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCGGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCCGTATCGATATGAACACCGACTCCACGCTGGAACCCGTGCCGAAACAGTTCGACGTTACCcgcg                                                                                                                  >  2:255242/1‑149 (MQ=255)

                            accaccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGCCCGCGCGTGACGCAAAAGTTTCGCGTATGCGTTTCGGTATAGATATGAACACCGACTACACCCTGTAAACATTGGCTAAACAGTTCGCCATTACCcgcaa                                                                                                                 >  2:139604/1‑147 (MQ=255)

                             ccaccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGaa                                                                                                                >  2:1002819/1‑149 (MQ=255)

                             ccaccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGaa                                                                                                                <  2:984216/149‑1 (MQ=255)

                                ccGACAGCCTGCGTGCGGCAACCCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGt                                                                                                             <  1:1061517/149‑1 (MQ=255)

                                  gAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTAt                                                                                                           <  1:9760/149‑1 (MQ=255)

                                      gCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGt                                                                                                       >  2:1230879/1‑149 (MQ=255)

                                         tgcgtgcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAg                                                                                                    >  1:924758/1‑149 (MQ=255)

                                             tgcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATcg                                                                                                >  1:344616/1‑149 (MQ=255)

                                             tgcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATACGTCAGAtgg                                                                                                >  1:918855/1‑147 (MQ=255)

                                               cgccAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATcgaa                                                                                              <  1:8101/146‑1 (MQ=255)

                                                  cAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGtt                                                                                                                                >  1:1369208/1‑112 (MQ=255)

                                                  cAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGtt                                                                                                                                <  2:1369208/112‑1 (MQ=255)

                                                     cGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATcgaagcgaag                                                                                        <  1:14078/149‑1 (MQ=255)

                                                      gCACGACGTGCTGGCTGGCCTGACCGCGCGGGAAGCAAAAGTGCTGCGTATGCGTTTCGGTATCGATATGAACTCCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAgg                                                                                       <  1:1180271/149‑1 (MQ=255)

                                                        acgacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCTTTATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgcg                                                                                     >  1:274905/1‑149 (MQ=255)

                                                          gacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAAc                                                                                                               <  2:608239/121‑1 (MQ=255)

                                                          gacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAAc                                                                                                               >  1:608239/1‑121 (MQ=255)

                                                          gacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCt                                                                                   >  1:1056313/1‑149 (MQ=255)

                                                           acgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTg                                                                                  <  2:148783/149‑1 (MQ=255)

                                                            cgTGATGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTgc                                                                                 >  2:1071924/1‑149 (MQ=255)

                                                                 tggctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCaaa                                                                            <  2:1322977/149‑1 (MQ=255)

                                                                   gctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACt                                                                          >  2:1140021/1‑149 (MQ=255)

                                                                   gctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTGCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTGACCCGCGGACGTATCCGTCAGATCGAAGTGAAGGCGCTGCGCAAACt                                                                          >  1:1071673/1‑149 (MQ=255)

                                                                       gcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGt                                                                      <  2:344616/149‑1 (MQ=255)

                                                                       gcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGt                                                                      <  1:853115/149‑1 (MQ=255)

                                                                       gcCTGACCGCGCGGGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGt                                                                      <  1:375953/149‑1 (MQ=255)

                                                                         cTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCa                                                                    <  1:415455/149‑1 (MQ=255)

                                                                           gACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCAcc                                                                  <  2:1325922/149‑1 (MQ=255)

                                                                            aCCGCGCGTGAAGGAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGCATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCAccc                                                                 <  1:1013850/149‑1 (MQ=255)

                                                                              cgcgcgTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCAcccca                                                               >  1:307766/1‑147 (MQ=255)

                                                                                  cgTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACGGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCg                                                           >  2:1374238/1‑149 (MQ=255)

                                                                                     gAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATcc                                                                                                         <  2:951801/100‑1 (MQ=255)

                                                                                     gAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATcc                                                                                                         >  1:951801/1‑100 (MQ=255)

                                                                                        gaaaaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGa                                                     <  2:440881/147‑1 (MQ=255)

                                                                                         cAAAAGTTCTGCGTATGCTTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGaa                                                    <  1:1025584/149‑1 (MQ=255)

                                                                                         cAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGaa                                                    <  2:1305601/149‑1 (MQ=255)

                                                                                         cAAAAGTTCGGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGaa                                                    <  2:776376/149‑1 (MQ=255)

                                                                                          aaaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCCAACTGCGTCACCCGAGCCGTTCTGAAg                                                   >  1:751204/1‑149 (MQ=255)

                                                                                           aaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGt                                                  >  2:1277955/1‑149 (MQ=255)

                                                                                           aaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGt                                                  <  1:35453/149‑1 (MQ=255)

                                                                                           aaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGt                                                  >  1:605770/1‑149 (MQ=255)

                                                                                                tcggggTATGGGTTTCGGGAGCGATATGAATACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGAGACGTTGGTTACCCGAGCCGTTCTGAAGtgctgc                                             <  2:307766/144‑1 (MQ=255)

                                                                                                    cGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAg                                         >  1:667884/1‑149 (MQ=255)

                                                                                                       aTGCGTTTCGGTATCGATATGTACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtgctg                                              <  1:1206741/141‑1 (MQ=255)

                                                                                                       aTGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAACCGTTCTGACGTGCTGCGTAGCtt                                      >  2:830261/1‑149 (MQ=255)

                                                                                                          cGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCt                                   <  1:1002819/149‑1 (MQ=255)

                                                                                                           gTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTg                                  >  1:368083/1‑149 (MQ=255)

                                                                                                            tttCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGt                                                          <  1:1363838/124‑1 (MQ=255)

                                                                                                            tttCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGt                                                          >  2:1363838/1‑124 (MQ=255)

                                                                                                            tttCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTgg                                 <  1:452179/149‑1 (MQ=255)

                                                                                                             ttCGGTATCGATATGAACACCGACTACACGCTGGAAGCCGTGGGTAAACAGTGCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGa                                <  1:970022/149‑1 (MQ=255)

                                                                                                             ttCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGa                                <  1:1310430/149‑1 (MQ=255)

                                                                                                                 gTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGAt                            >  1:426143/1‑149 (MQ=255)

                                                                                                                 gTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGAt                            >  1:297260/1‑149 (MQ=255)

                                                                                                                 gTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGAt                            <  1:982888/149‑1 (MQ=255)

                                                                                                                     cGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAAt                        <  1:235983/149‑1 (MQ=255)

                                                                                                                      gATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATc                       >  2:723733/1‑149 (MQ=255)

                                                                                                                       atatGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCg                      <  1:1192593/149‑1 (MQ=255)

                                                                                                                       atatGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACACGCGACCGTATCCGGCAGATCGAGGCGAAGGCGATGGGCACACTGAGTAACCCCAGCCGTTCTGAAGTGATCGGTAGCTTCATGGAAGATTACTCg                      >  1:996386/1‑149 (MQ=255)

                                                                                                                        taggAACACCGACTACACGCGGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCgg                     <  2:323344/146‑1 (MQ=255)

                                                                                                                          tGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGACTAATCGGTa                   >  1:1200493/1‑149 (MQ=255)

                                                                                                                            aaCACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCGGCGAACGTATCCGCCTGAACGAAGCTAAGGCGCTGCGCAAACTGCGGCACCCGAACCGTTCTGAAGTGCTGCTTAGCTACCTGGACGATTTATCGGAAgg                 >  2:715300/1‑149 (MQ=255)

                                                                                                                             acacCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTGGGc                >  2:453795/1‑149 (MQ=255)

                                                                                                                                ccGACTACACGCTGGAAGCAGTGAGTAAATATTACGACGTGAATCGGGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCGGCAGTGGTGCGGGGCTTCCTCGACACTTCATGCGGAGGCCgg             >  2:870960/1‑149 (MQ=255)

                                                                                                                                 cGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGa            >  1:557450/1‑149 (MQ=255)

                                                                                                                                 cGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCCGa            >  2:441448/1‑149 (MQ=255)

                                                                                                                                   aCTACACGCTGGAAGCAGTGGTTAAACAGTTCGACGTTACCCGCGAACGTATCCCTCAAATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATc          <  1:685211/149‑1 (MQ=255)

                                                                                                                                      acacGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAgg       <  2:1262840/149‑1 (MQ=255)

                                                                                                                                       cacGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTACCTTCCTCGCCGAGTAATCGGTAGTCCGGATCAGGc      >  2:328891/1‑149 (MQ=255)

                                                                                                                                       cacGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAAATGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGc      <  1:1071924/149‑1 (MQ=255)

                                                                                                                                        acGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCg     >  2:898542/1‑149 (MQ=255)

                                                                                                                                        acGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCg     >  1:1259178/1‑149 (MQ=255)

                                                                                                                                          gCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACg                              <  2:695108/122‑1 (MQ=255)

                                                                                                                                          gCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACg                              >  1:695108/1‑122 (MQ=255)

                                                                                                                                          gCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGtt   >  1:1207140/1‑149 (MQ=255)

                                                                                                                                           cTGGAAGCAGCGGGTAAACAGTTGGACGGTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGGGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTa  <  2:667884/149‑1 (MQ=255)

CACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTA  >  NC_000913_3_pae_pgi/3214624‑3214911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`