Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,731,621 G→T M845I (ATG→ATT)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,731,6210GT98.7% 251.1 / NA 79M845I (ATG→ATT) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (46/31);  total (46/33)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTA  >  NC_000913_3_pae_pgi/1731476‑1731765
                                                                                                                                                 |                                                                                                                                                 
aTCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGt                                                                                                                                                >  1:569116/1‑149 (MQ=255)
 tgcccccccGCTTTGGCAACAACGGTTGCTCCCCAACCAGTTGCTGGAAAGCGCTGAGGGAGAACCGGATTTCCCGGTCATTCTCGACCCCCTACGCGAATGTCTGCAACATGTTTATGATCTCCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                               <  2:935054/144‑1 (MQ=255)
 tCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                               <  1:1120520/149‑1 (MQ=255)
 tCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                               >  2:423648/1‑149 (MQ=255)
   gCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                             >  2:753380/1‑149 (MQ=255)
       cccGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGa                                                                                                                                         >  1:548828/1‑149 (MQ=255)
              tGGCAACAACGGCCGCCCGCCACTCAGTTGCTGGAAATCGCTCAAGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTgg                                                                                                                                  <  1:99625/149‑1 (MQ=255)
                    caacGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAAt                                                                                                                            <  2:468495/149‑1 (MQ=255)
                     aacGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAAtt                                                                                                                           >  2:362512/1‑149 (MQ=255)
                           tGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAata                                                                                                                     <  2:715208/149‑1 (MQ=255)
                           tGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAata                                                                                                                     >  1:588520/1‑149 (MQ=255)
                           tGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAata                                                                                                                     >  1:701289/1‑149 (MQ=255)
                               cgcCAGTCATTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCg                                                                                                                 <  2:79603/149‑1 (MQ=255)
                                gcCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCACGATGTTTATGAGCTTCTCGCACTGCAACGTTTGATTCGTCGCCTGCACGGTAACGAAATTCAAATATCCgc                                                                                                                >  1:294855/1‑148 (MQ=255)
                                gcCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGa                                                                                                                <  2:548828/149‑1 (MQ=255)
                                  cagtcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATg                                                                                                              >  1:497764/1‑149 (MQ=255)
                                  cagtcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATg                                                                                                              >  1:872032/1‑149 (MQ=255)
                                     tcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGACTGTCCGCACGATGTGGATGAGCTTCCCGCACTGGAACGGTTGAGTCGTAGCCTGAACGGTGGCGAAATTCAAATATCCGAGGTaa                                                                                                           >  2:935529/1‑149 (MQ=255)
                                        gtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGCGGCGAAATTCACATATCCGCTGTAACGa                                                                                                        >  2:711803/1‑149 (MQ=255)
                                        gtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGGTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGa                                                                                                        <  1:1073936/149‑1 (MQ=255)
                                            cTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCAc                                                                                                    >  1:953069/1‑149 (MQ=255)
                                            cTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCAc                                                                                                    >  1:1053902/1‑149 (MQ=255)
                                             tGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGa                                                                                                                >  1:413902/1‑136 (MQ=255)
                                             tGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGa                                                                                                                <  2:413902/136‑1 (MQ=255)
                                             tGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACt                                                                                                   <  1:266646/149‑1 (MQ=255)
                                             tGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACt                                                                                                   >  1:1277841/1‑149 (MQ=255)
                                                 aaTCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGc                                                                                              <  1:423648/149‑1 (MQ=255)
                                                  aTCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGcc                                                                                             >  2:428284/1‑149 (MQ=255)
                                                  aTCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGcc                                                                                             >  2:219234/1‑149 (MQ=255)
                                                   tCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                                                                               >  2:241314/1‑131 (MQ=255)
                                                   tCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                                                                               <  1:241314/131‑1 (MQ=255)
                                                          gggATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAGTTAACGACCACT‑ACGCCCTCGCCtt                                                                                     >  2:1095174/1‑149 (MQ=255)
                                                            gATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGAGGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTc                                                                                   <  1:1254695/149‑1 (MQ=255)
                                                               atCCGGATTTTCCGGTCATTCTCGAAACACTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGTACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCCCCTTTCGcc                                                                                >  2:937086/1‑149 (MQ=255)
                                                                  cGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCcaca                                                                             >  1:408774/1‑149 (MQ=255)
                                                                  cGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCcaca                                                                             >  1:1141012/1‑149 (MQ=255)
                                                                  cGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACAACCACT‑ACGCCCTCGCCTTTCCCccca                                                                             >  2:227099/1‑149 (MQ=255)
                                                                   ggATTTTCCGTTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACaa                                                                            <  2:1040342/149‑1 (MQ=255)
                                                                   ggATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAAGTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGacacca                                                                            >  2:303481/1‑147 (MQ=255)
                                                                   ggATTTTCCGGTCATTCCAGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACaa                                                                            <  1:59362/149‑1 (MQ=255)
                                                                       tttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGttt                                                                        <  1:428284/149‑1 (MQ=255)
                                                                            ggTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTTGCCCCAAGTTTAtttt                                                                   >  1:939360/1‑147 (MQ=255)
                                                                            ggTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGt                                                                   >  1:384375/1‑149 (MQ=255)
                                                                            ggTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGt                                                                   >  1:389115/1‑149 (MQ=255)
                                                                             gTCATTCTCGAACCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGtt                                                                  <  1:423647/149‑1 (MQ=255)
                                                                              tCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc                                                                 <  2:588520/149‑1 (MQ=255)
                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTGCGCTCAAGTTTATTGTTCgg                                                               >  1:832831/1‑149 (MQ=255)
                                                                                aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCgg                                                               <  2:384375/149‑1 (MQ=255)
                                                                                  tctcGAAACGCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt                                                             <  2:687235/149‑1 (MQ=255)
                                                                                  tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGTCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt                                                             >  1:782107/1‑149 (MQ=255)
                                                                                    tcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAt                                                           >  2:754869/1‑149 (MQ=255)
                                                                                    gcGAAACCGTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGCACGGTGGCGAAATGCAATTATCCGATGTAACGACCACT‑AAGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGTCTAt                                                           >  2:1297856/2‑149 (MQ=255)
                                                                                        aaCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTcg                                                       >  1:570863/1‑149 (MQ=255)
                                                                                          cccTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTcgcg                                                     <  2:570863/149‑1 (MQ=255)
                                                                                                 cgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTa                                              <  1:862702/149‑1 (MQ=255)
                                                                                                  gAATGTCTGCAAGACGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTAt                                             <  2:1381655/149‑1 (MQ=255)
                                                                                                   aaTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGAAGCGTTGAGAATATCCGACGTAAGTCCCAGT‑TCGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATg                                            <  1:334721/149‑1 (MQ=255)
                                                                                                         tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTGTGTACCag                                      >  2:446805/1‑149 (MQ=255)
                                                                                                          gCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATTTACCaga                                     >  1:950914/1‑149 (MQ=255)
                                                                                                              gATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGa                                 <  1:1217793/149‑1 (MQ=255)
                                                                                                              gATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGa                                 <  1:129915/149‑1 (MQ=255)
                                                                                                                  tttATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGcc                             >  1:825203/1‑149 (MQ=255)
                                                                                                                   ttATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGTTGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGACTTTATGTACAAGAGGGACGccc                            >  2:718122/1‑149 (MQ=255)
                                                                                                                    tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTCTTGTTCGGCTATGTCGCGGAATTTATGGACCAGCGCGACGcccc                           >  2:700870/1‑149 (MQ=255)
                                                                                                                       gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCt                        >  1:807799/1‑149 (MQ=255)
                                                                                                                       gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATGCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGCCTATGTCGCGAAATTTAAGTACCAAAGCAACGCCCCGcg                        >  2:364172/1‑148 (MQ=255)
                                                                                                                        aTCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGCGCGACGCCCCGCTg                       >  2:427617/1‑149 (MQ=255)
                                                                                                                        aTCTTCATGCCCAGGAACGTTTGATTCGTCTCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTg                       >  2:948979/1‑149 (MQ=255)
                                                                                                                         tCTTCCCGCCCTGGAACGTTTGATCTGTCGACCGCCCGGTAGCGTGGTTCATATATCCGATGTAAC‑ACAATTCTCTCCCTCCCCTGCCGATGGTATTTTATGGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTgg                      <  1:1383630/149‑1 (MQ=255)
                                                                                                                          cTTCCCGCACTGGAACGTTTAGTTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGc                     <  2:424247/149‑1 (MQ=255)
                                                                                                                           ttCCAGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCa                    <  1:711803/149‑1 (MQ=255)
                                                                                                                               cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAgc                >  1:1333860/1‑149 (MQ=255)
                                                                                                                                  aCTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGcc             >  1:191472/1‑149 (MQ=255)
                                                                                                                                   cTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCg            <  2:782107/149‑1 (MQ=255)
                                                                                                                                    tGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCgg           <  1:15053/149‑1 (MQ=255)
                                                                                                                                      gAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCCATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGc         >  1:1398655/1‑149 (MQ=255)
                                                                                                                                      gAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATGCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCCGAGCGCCGGGc         >  1:302746/1‑149 (MQ=255)
                                                                                                                                             tGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTa  <  2:540951/149‑1 (MQ=255)
                                                                                                                                             cgATTCGCCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACACGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTa  <  1:738498/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT‑ACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTA  >  NC_000913_3_pae_pgi/1731476‑1731765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑