Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,440,176 G→A T285I (ACC→ATC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,440,1760GA98.4% 190.5 / NA 62T285I (ACC→ATC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/33);  minor base T (0/1);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACA  >  NC_000913_3_pae_pgi/3440034‑3440322
                                                                                                                                              |                                                                                                                                                  
aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTg                                                                                                                                             >  2:260970/1‑150 (MQ=255)
       tACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGa                                                                                                                                      <  2:646718/150‑1 (MQ=255)
          tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTg                                                                                                                                             >  1:516708/1‑140 (MQ=255)
          tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTg                                                                                                                                             <  2:516708/140‑1 (MQ=255)
          tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAAAGCATTTTTACCAAGGTTAGGCGTTTTAAGGAGCCCAACCTCAATAGGCTGTCCCAGATcc                                                                                                                                   >  1:468205/1‑149 (MQ=255)
               aGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGat                                                                                                                              >  2:10452/1‑150 (MQ=255)
                  gATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCCCAGAAAAACAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCTACGTTAGGCGGTTTATGTAGCTGAACCTCGATACGCTGTACCAAATCAACGAtata                                                                                                                           >  2:353700/1‑150 (MQ=255)
                  gATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGAtata                                                                                                                           >  2:312109/1‑150 (MQ=255)
                     gttggCCGGTGGCCAGTTTTCCAGGCGCAGTGCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTg                                                                                                                        <  2:127590/146‑1 (MQ=255)
                     gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTg                                                                                                                        <  1:801480/150‑1 (MQ=255)
                     gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTg                                                                                                                        <  2:584192/150‑1 (MQ=255)
                     gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTg                                                                                                                        <  1:538378/150‑1 (MQ=255)
                     gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTg                                                                                                                        >  2:95292/1‑150 (MQ=255)
                      cTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTgg                                                                                                                       <  2:697783/150‑1 (MQ=255)
                          ccGGTGGCCAGTTTTCCAGACGCATGCCCAGAGACAGTCCACGGGAAGACAGCACGTCTTTAAGCTGAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAg                                                                                                                   <  1:214557/150‑1 (MQ=255)
                            ggTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCt                                                                                                                 <  1:356291/150‑1 (MQ=255)
                                 ccAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc                                                                                                            <  1:260970/150‑1 (MQ=255)
                                   aGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCtt                                                                                                          >  1:882425/1‑150 (MQ=255)
                                      tttCCAGGCGGATGCCCAGAGACAGACCGTGGGAAGCAAGCACGCCTTGAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa                                                                                                       <  2:967281/150‑1 (MQ=255)
                                          cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa                                                                                                   <  1:312109/150‑1 (MQ=255)
                                           aGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACAGAGTACCAGGCCACCTATATAGTGGACAGCTTCTGCTTTaaaccaa                                                                                                  >  1:273164/1‑145 (MQ=255)
                                                     ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc                                                                                        <  1:95292/150‑1 (MQ=255)
                                                     ccAGAGACAGTCCACGGGAAGACAGCACGTCTTTAATCTCAGTACGTGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGACACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc                                                                                        <  1:419634/150‑1 (MQ=255)
                                                          gaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGGGGATAGCTTCTGCTTTAAGCCAGTTACCAGAGCggccc                                                                                   >  2:354373/1‑147 (MQ=255)
                                                           aCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATATTGGATAGCTTCTGCTTTACGGCAGTTAGCAGGGCGGACAg                                                                                  >  2:23100/1‑150 (MQ=255)
                                                            cAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGt                                                                                 <  2:862393/150‑1 (MQ=255)
                                                                  aCGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGc                                                                                                                  <  1:728429/111‑1 (MQ=255)
                                                                  aCGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGc                                                                                                                  >  2:728429/1‑111 (MQ=255)
                                                                    gggAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCa                                                                         <  2:843434/150‑1 (MQ=255)
                                                                          ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCCGATCGt                                                                   >  2:312191/1‑150 (MQ=255)
                                                                               aCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACa                                                              <  1:91926/150‑1 (MQ=255)
                                                                                 gTCTTTAATTTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAgg                                                            >  1:856310/1‑150 (MQ=255)
                                                                                 gTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc                                                                                        <  1:578048/122‑1 (MQ=255)
                                                                                 gTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc                                                                                        >  2:578048/1‑122 (MQ=255)
                                                                                    tttAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgcg                                                         >  1:198970/1‑150 (MQ=255)
                                                                                    tttAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGAACAGTCAATTCCAGATCGTCAACAGGgcg                                                         >  2:288432/1‑150 (MQ=255)
                                                                                           tcAGTAAGAGATTTTTTACCACGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAgg                                                  >  2:868628/1‑150 (MQ=255)
                                                                                             aGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCACCAGGGCGCAGCAggct                                                >  2:131270/1‑148 (MQ=255)
                                                                                             aGTAAGAGATTTTTTACCAAGGTTAGGCCTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCGTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggat                                                <  1:947335/150‑1 (MQ=255)
                                                                                                 agatATTCTTTCCCAAGGTTCGGCATTGTAAGGAGCTAAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCAGACAGTCAAGTCCAGATCGTCAACAGGGCGCAGCAggatcgga                                            <  2:57831/150‑1 (MQ=255)
                                                                                                 agagATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAACAggatcgga                                            <  2:882425/150‑1 (MQ=255)
                                                                                                  gagaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggat                                           <  1:194672/150‑1 (MQ=255)
                                                                                                   agaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggatc                                          <  1:354373/150‑1 (MQ=255)
                                                                                                          ttACCCAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActct                                   <  1:353700/150‑1 (MQ=255)
                                                                                                             ccAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGAAACGAATCGAACTCTGGt                                <  2:856310/150‑1 (MQ=255)
                                                                                                              cAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt                                                                                                 <  1:581715/84‑1 (MQ=255)
                                                                                                              cAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt                                                                                                 >  2:865905/1‑84 (MQ=255)
                                                                                                              cAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt                                                                                                 >  2:581715/1‑84 (MQ=255)
                                                                                                              cAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt                                                                                                 <  1:865905/84‑1 (MQ=255)
                                                                                                              cAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGGTCGGATCGAACTCTGGtt                               >  1:83621/1‑150 (MQ=255)
                                                                                                              cAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGtt                               >  2:756990/1‑150 (MQ=255)
                                                                                                                     aGGCGTTTTAAGGAGCTCATCTTGGTAACGCTGTACACGATAACCGATATAGGGGATCGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCACGATCGGATCGAACTCTGGTTTCtcttc                        <  2:417330/150‑1 (MQ=255)
                                                                                                                       gCGTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTcttctt                      >  2:41912/1‑150 (MQ=255)
                                                                                                                        cggtgtAAGGGGCTGAACCTGGATACGGGGGAGCAGGTGAGCGAGAGAGTGGAGAGCTTCGGCTTGAAGGCAGTGAGGAGAGCGGACGGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCttt                     <  2:848195/145‑1 (MQ=255)
                                                                                                                         gTTTTAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTc                    >  1:880857/1‑150 (MQ=255)
                                                                                                                          ttttAAGGGGCTCAACCTCGATAGGCGGTAACAGATCGCCGATGTAGTGGATGGCTTCTGCTTTAAGGCAGTTAGCAGAAAGAAAAGAAAATTCAAGATCATCAACAGGGCGAAGCAGGATCGGATCCAGATCAGGTTTCTCTGCTTTCa                   >  2:196190/1‑150 (MQ=255)
                                                                                                                           tttAAGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCAc                  <  1:508826/150‑1 (MQ=255)
                                                                                                                             tAAGGAGCCCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACtt                <  2:887483/150‑1 (MQ=255)
                                                                                                                               aGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcg                                              <  1:847164/118‑1 (MQ=255)
                                                                                                                               aGGAGCTCAACCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcg                                              >  2:847164/1‑118 (MQ=255)
                                                                                                                                        aCCTCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGt     <  2:198970/150‑1 (MQ=255)
                                                                                                                                           tCGATACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCCGATCGTCAACAGGGCGCCGCAGGATCGGATCGAACTCTGGTTTCACTTCTTGCCCTTCGCGCTGCCGtgcg  >  1:259182/1‑147 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
ATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACA  >  NC_000913_3_pae_pgi/3440034‑3440322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: