Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_pgi 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_pgi 257908 258683 776 59 [0] [0] 59 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi = 2579070 (0.000)56 (1.060) 47/266 0.2 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_pae_pgi 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

CGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTC  >  NC_000913_3_pae_pgi/258535‑258832
                                                                                                                                                     |                                                                                                                                                     
acgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatatgcgtgaaggtaa                                                                                                                                                       <  1:607608‑M2/1‑1 (MQ=255)
  gcttgtgttaaaaatctgctaaattttgccaatttggtaaaacagttgcatcagaaaaggagatagcaatgacgtgaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactcggcccgtatattcgtgaaggtaAGt                                                                                                                                                     <  2:41941‑M2/3‑1 (MQ=255)
   cttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                                                                    <  1:142730‑M2/4‑1 (MQ=255)
       tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                                                                                <  1:18637‑M2/8‑1 (MQ=255)
         ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggccacccgaagagcagattgatcaaaaaatttaccgccctaggcccgtatattcgtgacggtaAgtgcacgac                                                                                                                                              >  2:719612‑M2/141‑146 (MQ=255)
         ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagaaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatgttcgtgaaggtaAGTGCAgaga                                                                                                                                              >  1:29829‑M2/141‑150 (MQ=255)
          accaaattggcaaaatttagcaaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                                                                             >  1:693088‑M2/140‑150 (MQ=255)
           aaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATa                                                                                                                                            >  1:797782‑M2/139‑150 (MQ=255)
                               caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCgatttt                                                                                                                        >  2:78740‑M2/119‑148 (MQ=255)
                                       taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                                                                     <  1:144814‑M2/3‑1 (MQ=255)
                                       taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                                                                     >  2:144814‑M2/111‑113 (MQ=255)
                                         aaacagttgcatcacaacaggagatcgcaatgacgtttccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                                                                              <  1:676349‑M2/42‑1 (MQ=255)
                                         aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                                                                              >  2:792383‑M2/109‑150 (MQ=255)
                                         aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                                                                              >  1:679583‑M2/109‑150 (MQ=255)
                                             agttgcatcacaacagtagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatcttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                          <  1:928489‑M2/46‑1 (MQ=255)
                                             agttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                          <  2:816440‑M2/46‑1 (MQ=255)
                                                 gcagcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAc                                                                                                      <  1:411234‑M2/50‑1 (MQ=255)
                                                    tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                                  >  2:814463‑M2/98‑150 (MQ=255)
                                                       caacaggagatagcaatgacgttaccaagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggaccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGCTTGTCTTGCTGTCTGACTCAACGTGaaa                                                                                               >  2:61917‑M2/95‑150 (MQ=255)
                                                         cgatctgatagcaatgacgtgaccgggtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                             <  2:391223‑M2/58‑1 (MQ=255)
                                                           aggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                           <  1:934766‑M2/60‑1 (MQ=255)
                                                             gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                          >  1:393793‑M2/89‑118 (MQ=255)
                                                             gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                          <  2:393793‑M2/30‑1 (MQ=255)
                                                                atagcaatgacgttaccgagtggacccccgaagagcagattgatccaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTgaaaccgagatgg                                                                                      >  1:391223‑M2/86‑144 (MQ=255)
                                                                     aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                                 >  2:596952‑M2/81‑150 (MQ=255)
                                                                         aaggtaccgagtggacccccgaagagcaaagtgatcaaaaaatttaccgcacgaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                             <  2:100194‑M2/74‑1 (MQ=255)
                                                                           gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGa                                                                           >  1:281496‑M2/75‑150 (MQ=255)
                                                                                  agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                                    <  1:527307‑M2/83‑1 (MQ=255)
                                                                                   gtggacacccgaaggacagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                                                                   <  1:747898‑M2/84‑1 (MQ=255)
                                                                                   gtggacacccgaagagcagagtgatcaaaaaatttaccgcactatgcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTTAAACCGGCACCGGAAGTGCGTGAATTCTggg                                                                   <  1:163971‑M2/84‑1 (MQ=255)
                                                                                         acccgaagagcagattgataaaaaaagttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGctggg                                                             >  1:394293‑M2/61‑149 (MQ=255)
                                                                                            cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                          <  2:693088‑M2/93‑1 (MQ=255)
                                                                                             gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                         <  1:661305‑M2/94‑1 (MQ=255)
                                                                                              aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg                                                        <  1:596952‑M2/95‑1 (MQ=255)
                                                                                                  gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc                                                    >  2:504286‑M2/52‑150 (MQ=255)
                                                                                                    agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGACTAGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGATGGTGGATGGAGCTg                                                  >  1:264944‑M2/50‑150 (MQ=255)
                                                                                                      attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGTTTGTTTGTCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGGATTCTGGGGCTGGTGGATGGAGCTGGa                                                >  2:834151‑M2/48‑150 (MQ=255)
                                                                                                      attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGATGGTGGATGGAGCTGGa                                                >  2:803926‑M2/48‑150 (MQ=255)
                                                                                                         gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                           <  2:892707‑M2/92‑1 (MQ=255)
                                                                                                         gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                           >  1:892707‑M2/45‑136 (MQ=255)
                                                                                                          atcaaaaaatttaccgcactagtcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcg                                            >  1:745376‑M2/44‑150 (MQ=255)
                                                                                                             aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg                                                        <  2:275962‑M2/95‑1 (MQ=255)
                                                                                                             aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg                                                        >  1:275962‑M2/41‑135 (MQ=255)
                                                                                                                  agttaccgcacagggcccgtatattcgtgaaggtaAGTGCAAAGATAAGCGATTCGTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATc                                    <  2:106764‑M2/115‑1 (MQ=255)
                                                                                                                   tttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCATAGTGAAACAGGCACCCGAATTGCGTGAATTGTGGTGATTGTGGATGGGGCTGCAAGCGCAGGGATcc                                   >  2:152366‑M2/35‑150 (MQ=255)
                                                                                                                      accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                                <  1:16030‑M2/119‑1 (MQ=255)
                                                                                                                       ccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                               <  1:814463‑M2/120‑1 (MQ=255)
                                                                                                                        cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGttt                              >  1:568239‑M2/30‑150 (MQ=255)
                                                                                                                         gcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtttt                             >  1:449498‑M2/29‑150 (MQ=255)
                                                                                                                           actagtcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                           <  2:745376‑M2/124‑1 (MQ=255)
                                                                                                                            ctaggcccggatattcttgaaggctAATGAAAAGATAATCTATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAcc                          <  1:476041‑M2/125‑1 (MQ=255)
                                                                                                                              aggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa                        <  2:568239‑M2/127‑1 (MQ=255)
                                                                                                                                  ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt                    >  1:879149‑M2/20‑150 (MQ=255)
                                                                                                                                         ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGt             >  1:829553‑M2/13‑150 (MQ=255)
                                                                                                                                          tcgggaaggtaAGTGCAAAGTTAATCGATTCTTTTGCGATTGTCTGGTTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGCTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGtt            <  2:317835‑M2/139‑1 (MQ=255)
                                                                                                                                            gtgaaggtaAGTGCAAAGATAACCGATTCTTTTTCGATTATCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCGGGAAGCGCAGGAATCCCGCTTTACCTCCAGTTACCAGTTTg          >  2:608628‑M2/10‑150 (MQ=255)
                                                                                                                                                   taAGTGCAAAGATAATCGATTCCTTTTCGATTGTCTGGCTGTTTGCTGATTTTGGAAACCGGCACCGGACGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt   <  1:719612‑M2/148‑1 (MQ=255)
                                                                                                                                                   taAATGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt   <  1:569233‑M2/148‑1 (MQ=255)
                                                                                                                                                    aAGTGTAAAGATAATCTATTCTTTTTCGATGGTCTGGTTGTATGCGTAAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACTTACAGTTACCAGTTTGGTCTGTTc  <  2:826707‑M2/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTC  >  NC_000913_3_pae_pgi/258535‑258832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.