Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,731,621 G→T M845I (ATG→ATT lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,731,6210GT100.0% 17.8 / NA 9M845I (ATG→ATTlhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/3);  total (6/3)

ACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAG  >  NC_000913_3_pae_pgi/1731481‑1731750
                                                                                                                                            |                                                                                                                                 
aCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGTAACCCTTCGCGAATGTCTGCAAGATGTTTATTATCTTCCCGCACTGGAACGTTTGATTCGTCGCCt                                                                                                                           >  2:150155/1‑149 (MQ=255)
                                                 cTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCg                                                                          >  1:295016/1‑149 (MQ=255)
                                                  tCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACACTACGCGAATGTTTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGCAATTTAAATCTCCGCTGTAACGACCACTACGCACTCga                                                                         >  2:172213/1‑148 (MQ=255)
                                                                       ggTCATTCTCGAAACCCTACGCGAATGTCAGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGGAACGACCACTACGCCATCGCCTTTCCCCACAAGTTTAttag                                                    >  2:130803/1‑147 (MQ=255)
                                                                                                              ttATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGccc             <  2:18517/149‑1 (MQ=255)
                                                                                                               tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGGTGTAACGACAACTACGCCCTCGGCTTTCGCCACAAGTTTATTGTTCGGCTATGTCCCGGAATTTATGTACCAGAGCGACGcccc            >  2:113436/1‑149 (MQ=255)
                                                                                                                      tccccGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCCCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCa     <  1:171791/147‑1 (MQ=255)
                                                                                                                        cccGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCaga   <  1:13040/149‑1 (MQ=255)
                                                                                                                         ccGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCagag  >  2:266528/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                 
ACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAG  >  NC_000913_3_pae_pgi/1731481‑1731750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: