Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_pgi 1299499–1300697 1300697 1–1199 41 [0] [0] 41 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi = 12994980 (0.000)39 (1.020) 35/274 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACG  >  NC_000913_3_pae_pgi/1300550‑1300838
                                                                                                                                                    |                                                                                                                                            
ttctccaatttatgtcagttataagctttcccttaactgtctctcttcaccatcatgttattttcgccacatcataatcctcggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                              <  1:109805‑M2/1‑1 (MQ=255)
 tctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaat                                                                                                                                             <  2:209939‑M2/2‑1 (MQ=255)
     aaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                         >  2:562738‑M2/144‑149 (MQ=255)
             gttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTg                                                                                                                                 <  2:370199‑M2/14‑1 (MQ=255)
              ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtaggggaaagtgctaaATAATAATCAATTGt                                                                                                                                >  1:57024‑M2/135‑149 (MQ=255)
                  ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagattaattgaaacgatattattacttcccctgcccttagtataggacaatgttaaATAATAATCCACTGttaca                                                                                                                            >  2:160518‑M2/131‑147 (MQ=255)
                  ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTcaa                                                                                                                            <  2:9563‑M2/19‑1 (MQ=255)
                    gtaaggatttccttaactgcttctcctcagcatcttgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattttttccatttcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatt                                                                                                                          <  1:240808‑M2/21‑1 (MQ=255)
                      aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                        >  1:212405‑M2/127‑149 (MQ=255)
                         gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                     <  2:531691‑M2/26‑1 (MQ=255)
                              ccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtaggggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAtt                                                                                                                >  1:378409‑M2/119‑149 (MQ=255)
                                 taactgcttctcctcaccatcatgttattttcgccacaacataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                             >  2:608379‑M2/116‑149 (MQ=255)
                                  aactgattctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCac                                                                                                            <  2:70827‑M2/35‑1 (MQ=255)
                                      gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                        <  1:45798‑M2/39‑1 (MQ=255)
                                                  catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtaggggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                            <  2:378409‑M2/51‑1 (MQ=255)
                                                  catcatgttatattcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                            >  1:425235‑M2/99‑149 (MQ=255)
                                                         ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                   >  1:346020‑M2/92‑135 (MQ=255)
                                                         ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                   <  2:346020‑M2/44‑1 (MQ=255)
                                                             tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                 >  2:546902‑M2/88‑149 (MQ=255)
                                                                 gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATGGACAt                                                                             >  2:531620‑M2/84‑149 (MQ=255)
                                                                 gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                             >  2:518004‑M2/84‑149 (MQ=255)
                                                                 gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTCTAATCAGAAAAGATAGACAt                                                                             >  1:601804‑M2/84‑149 (MQ=255)
                                                                         ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATCGCCAGGCTTGgcg                                                                     <  2:181294‑M2/74‑2 (MQ=255)
                                                                                  ggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                            <  2:212405‑M2/83‑1 (MQ=255)
                                                                                  ggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATCCTTAGACAATCTCTAt                                                            >  1:314092‑M2/67‑149 (MQ=255)
                                                                                        ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                      <  1:257872‑M2/89‑1 (MQ=255)
                                                                                        cgcaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                      <  2:22600‑M2/89‑1 (MQ=255)
                                                                                           aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                   <  1:562738‑M2/92‑1 (MQ=255)
                                                                                                   ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAACCAATCTCTATTTGATTGAATTAAAAGa                                           >  2:570396‑M2/50‑149 (MQ=255)
                                                                                                      aaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgt                                        >  2:296749‑M2/47‑149 (MQ=255)
                                                                                                         tgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTAATTGAATTGGAAGatgtttc                                     >  2:273579‑M2/44‑148 (MQ=255)
                                                                                                             attattaagtccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGATAAGATAGACATGCTTTGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                 <  2:344390‑M2/110‑1 (MQ=255)
                                                                                                                      tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                       <  2:246186‑M2/24‑1 (MQ=255)
                                                                                                                      tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                       >  1:246186‑M2/31‑54 (MQ=255)
                                                                                                                          atgcctttgggagaagaaagtgctaaATAATAATCAATTGTTAAAATATTGTGTATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                    <  2:229784‑M2/123‑1 (MQ=255)
                                                                                                                            gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGc                  <  1:568118‑M2/125‑1 (MQ=255)
                                                                                                                               attgggagaggaaagtgctaaATAACAATCAAGTGTTAAATTATGGGTCATTTCACTCCAGAAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat               <  1:160518‑M2/128‑1 (MQ=255)
                                                                                                                                         gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAt     >  2:153988‑M2/12‑149 (MQ=255)
                                                                                                                                            agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg  <  2:390881‑M2/141‑1 (MQ=255)
                                                                                                                                                   aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                        <  1:27833‑M2/87‑1 (MQ=255)
                                                                                                                                                   aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                        >  2:27833‑M2/2‑88 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACG  >  NC_000913_3_pae_pgi/1300550‑1300838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: