Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 1,275,721 C→T E37K (GAA→AAA)  narL ← response regulator in two‑component regulatory system with NarX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi1,275,7210CT96.9% 206.1 / NA 64E37K (GAA→AAA) narLresponse regulator in two‑component regulatory system with NarX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/34);  minor base G (1/1);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACAATGCGCCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGG  >  NC_000913_3_KO_pgi/1275577‑1275866
                                                                                                                                                |                                                                                                                                                 
ccACAATGCGCCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGGGTTTCCAGAGCGTTCATGCCGGGCGTATTGGGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCAAGTTCAATACACTGTTCGCCTTTACTCGCTTTGccg                                                                                                                                               >  2:573805/1‑148 (MQ=255)
 cacaATGCGCCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATCACTCGCTTTGccca                                                                                                                                              >  2:734845/1‑147 (MQ=255)
 cacaATGCGCCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGAATCAACGAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCaa                                                                                                                                              >  1:165926/1‑149 (MQ=255)
        cgcCCTGAGAGGGACTTTTCGCGCAGTTTCTCCACCGTTTCCAGCCCGTTCATGCCGGGCATATTGAGATCTAACAGGAACAGATCTTTTTCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGt                                                                                                                                       <  1:436531/149‑1 (MQ=255)
        cgcCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGt                                                                                                                                       <  1:322354/149‑1 (MQ=255)
          cccTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGa                                                                                                                                     >  2:167534/1‑149 (MQ=255)
             tGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGatat                                                                                                                                  <  1:45033/149‑1 (MQ=255)
                 agGGACTTTTCGCTCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCTGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTgg                                                                                                                              <  2:417055/149‑1 (MQ=255)
                  aggACTCGTCGCGCAGTGTGTCGAGTGTTTCCAAAACGTTCATGCCGTGCATATTGATATCTAACAGGATCAGATCGGGATCACGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGt                                                                                                                             <  2:545727/148‑1 (MQ=255)
                   ggtcTTTCCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGACCGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGGCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTg                                                                                                                            <  2:316338/146‑1 (MQ=255)
                            gcgcAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGa                                                                                                                   >  1:253485/1‑149 (MQ=255)
                            gcgcAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGa                                                                                                                   >  2:535831/1‑149 (MQ=255)
                                  tttATCCCGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCt                                                                                                             <  1:116939/149‑1 (MQ=255)
                                   ttagctaGCGTTTCTAGACCGTTCATGCCGGGCATATTGAGATCTATCAGGATCAGATCGGGCTCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTg                                                                                                            <  2:106447/143‑1 (MQ=255)
                                       ccAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAAc                                                                                                                                             <  1:250366/112‑1 (MQ=255)
                                       ccAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAAc                                                                                                                                             >  2:250366/1‑112 (MQ=255)
                                        cAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGTATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTa                                                                                                       >  1:218758/1‑149 (MQ=255)
                                           cGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGc                                                                                                    <  1:900134/149‑1 (MQ=255)
                                           cGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGAGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGc                                                                                                    <  1:823550/149‑1 (MQ=255)
                                             tttCCATACGCTTCAAGACTGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCa                                                                                                  <  2:653937/149‑1 (MQ=255)
                                                ccAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTAATGGCTGGGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGtt                                                                                               >  1:888285/1‑149 (MQ=255)
                                                 cAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTc                                                                                              >  1:477577/1‑149 (MQ=255)
                                                  aGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGt                                                                                                           <  2:519293/135‑1 (MQ=255)
                                                  aGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGt                                                                                                           >  1:519293/1‑135 (MQ=255)
                                                  aGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCg                                                                                             <  1:280238/149‑1 (MQ=255)
                                                        ttCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCCACCACGGGGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCAt                                                                                       >  1:660880/1‑149 (MQ=255)
                                                          cATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCAt                                                                                       >  2:514992/1‑147 (MQ=255)
                                                          cATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCAt                                                                                       <  1:514992/147‑1 (MQ=255)
                                                          cATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCg                                                                                     >  1:584662/1‑149 (MQ=255)
                                                           attcctggCCTATCGATATCTACCAGGATCAGATCGGGATCAAGAGACTCCGCTAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACTACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCgg                                                                                    <  1:80175/143‑1 (MQ=255)
                                                             gCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGTCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCg                                                                                     <  2:398035/146‑1 (MQ=255)
                                                             gCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGTCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCg                                                                                     >  1:398035/1‑146 (MQ=255)
                                                                   cATATTGAGATCTAAGAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGt                                                                            <  2:397708/149‑1 (MQ=255)
                                                                      atTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCACCATCGGGGGATCGTCaa                                                                         >  2:85803/1‑149 (MQ=255)
                                                                        ggataTCTAACAGGATGAGATCGGTAACAAAGGTCTCCGCCAGTTAAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATc                                                                       <  2:24103/145‑1 (MQ=255)
                                                                                  cAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCCCAGCATCGGGTGATCGTCAATCACCAGGAtag                                                             >  2:566544/1‑149 (MQ=255)
                                                                                      aTCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGTTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGc                                                         <  2:401032/149‑1 (MQ=255)
                                                                                          gATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATAc                                                                                                                      <  1:576504/84‑1 (MQ=255)
                                                                                          gATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATAc                                                                                                                      >  2:576504/1‑84 (MQ=255)
                                                                                           aTCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGa                                                                >  1:487199/1‑137 (MQ=255)
                                                                                           aTCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGa                                                                <  2:487199/137‑1 (MQ=255)
                                                                                                          cTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATgg                                     <  1:728224/149‑1 (MQ=255)
                                                                                                          cTCCGCCAGTCCAATACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATgg                                     <  1:312135/149‑1 (MQ=255)
                                                                                                                    tCAATACCCTGTTCGCCATTACTCGCTCTGCCAACCACGGTGATATCTGGCGTTTAACTGATTAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATTGTTAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCtt                           <  1:888070/149‑1 (MQ=255)
                                                                                                                      aaTACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTAtct                               >  2:109555/1‑143 (MQ=255)
                                                                                                                      aaTACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTAtct                               <  1:109555/143‑1 (MQ=255)
                                                                                                                      aaTACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCCCCTTgg                         >  1:236033/1‑149 (MQ=255)
                                                                                                                      aaTACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCAGGTTCCGGCTTACTCATAGGTCTCTCTTTgg                         >  2:500003/1‑149 (MQ=255)
                                                                                                                        tACCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACAGATAAGCTGTTTTACGCCAGTTCGCAGCAACGGGTGATCGTCATTCAGCAGGATAGTCGCCGATACCTCCTTCGTCATGGGTATCTCTTTggct                       >  2:449855/1‑147 (MQ=255)
                                                                                                                         aCCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCgg                                                      <  1:121274/117‑1 (MQ=255)
                                                                                                                         aCCCTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCgg                                                      >  2:121274/1‑117 (MQ=255)
                                                                                                                           ccTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGtt                                                                                               >  2:304769/1‑74 (MQ=255)
                                                                                                                           ccTGTTCGCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGtt                                                                                               <  1:304769/74‑1 (MQ=255)
                                                                                                                            cttttCGCCATTCCTCGCTTGGCCAACCACGGTGATATTTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCt                   <  1:192183/146‑1 (MQ=255)
                                                                                                                                  gcatttACTCGTTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGaaa             <  2:318535/145‑1 (MQ=255)
                                                                                                                                  gCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGaaa             >  1:82205/1‑149 (MQ=255)
                                                                                                                                  gCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGaaa             >  1:82278/1‑149 (MQ=255)
                                                                                                                                  gCCATTACTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCCCCTTGGACGTCTGTGaaa             <  2:236033/149‑1 (MQ=255)
                                                                                                                                     aTTACTCGCTTTGCCAAGCTCGGTTATATTTGGTGCACTACTGTTAAGTTGTTTTACGCCAGGTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGtt          <  2:117020/149‑1 (MQ=255)
                                                                                                                                        aCTCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGttttt       <  1:535831/149‑1 (MQ=255)
                                                                                                                                          tCGCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc     >  2:844523/1‑149 (MQ=255)
                                                                                                                                            gCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCgg   >  1:784546/1‑149 (MQ=255)
                                                                                                                                            gCTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCgg   <  1:854051/149‑1 (MQ=255)
                                                                                                                                             cTTTGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCggg  <  2:253485/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CCACAATGCGCCCTGAGAGGGACTTTTCGCGCAGTTTATCCAGCGTTTCCAGACCGTTCATGCCGGGCATATTGAGATCTAACAGGATCAGATCGGGATCAAGAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGG  >  NC_000913_3_KO_pgi/1275577‑1275866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑