Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	1978503	1979278	776	48 [0]	[0] 49	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 1978502	0 (0.000)	47 (0.980)	40/270	0.2	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_KO_pgi	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913_3_KO_pgi/1978358‑1978648                                                                                                                                                 |                                                                                                                                                   aaggggtctTGAGACTGATTTAAAGGTTTTCCGAAATGGACGCAACTGTACTCGTAACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTTATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtata                                                                                                                                                <  2:703933‑M1/146‑5 (MQ=255)  aTGTGTCTTTCCACTTATCTAATAATTTTCCTAAATCGACGCAACTGTACTTGTCACTACACGCATATACAACGGAGGGGTGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                               <  2:223157‑M1/149‑6 (MQ=255)      gTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtca                                                                                                                                           >  1:301377‑M1/1‑140 (MQ=255)       tCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcat                                                                                                                                          <  1:71012‑M1/149‑11 (MQ=255)       tCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcat                                                                                                                                          >  1:25739‑M1/1‑139 (MQ=255)               cTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgt                                                                                                                                  <  2:853764‑M1/149‑19 (MQ=255)                 tATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttt                                                                                                                                <  2:746467‑M1/149‑21 (MQ=255)                    cTAAGATTTTTCCTAAATCGACGCAATTGTACTCGTTACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                             <  1:562927‑M1/149‑24 (MQ=255)                    cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                             <  1:483341‑M1/149‑24 (MQ=255)                     tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGCTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttggttttggg                                                                                                                            >  1:859017‑M1/1‑125 (MQ=255)                     tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttcgg                                                                                                                            >  1:417133‑M1/1‑125 (MQ=255)                     tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtcttggg                                                                                                                            >  2:649694‑M1/1‑125 (MQ=255)                     tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGCGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatcctttttatttggg                                                                                                                            >  2:80307‑M1/1‑125 (MQ=255)                      aaGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactt                                                                                                                                      >  2:83332‑M1/1‑124 (MQ=255)                      aaGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactt                                                                                                                                      <  1:83332‑M1/138‑15 (MQ=255)                      aaGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACAATGAATTACTTAACATAAATGtataagtcatacttttgtcttgggg                                                                                                                           >  2:800236‑M1/1‑124 (MQ=255)                         aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCCATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                                                                        >  1:234126‑M1/1‑121 (MQ=255)                         aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                                                                        <  2:13060‑M1/149‑29 (MQ=255)                         aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTACGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggatgta                                                                                                                        >  1:764515‑M1/1‑121 (MQ=255)                                cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                 >  2:89288‑M1/1‑114 (MQ=255)                                cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                 <  1:373048‑M1/149‑36 (MQ=255)                                     tCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtt                                                                                                            >  1:488807‑M1/1‑109 (MQ=255)                                            aaCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtgaaaaagt                                                                                                     >  1:546209‑M1/1‑102 (MQ=255)                                                   cTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtgttgggtgtatttctatctgttaaaaagtttttcat                                                                                              >  2:859710‑M1/1‑95 (MQ=255)                                                      gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctac                                                                                           <  1:649757‑M1/149‑58 (MQ=255)                                                        cACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc                                                                                         <  2:764515‑M1/149‑60 (MQ=255)                                                         aCTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATGACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaaactgttaaaaagtttttcgctacgct                                                                                        >  2:21724‑M1/1‑89 (MQ=255)                                                           tACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                                 >  1:874404‑M1/1‑87 (MQ=255)                                                           tACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtgttgggtgtatttcaatctgttaaaaagttttt                                                                                                 <  2:874404‑M1/138‑52 (MQ=255)                                                                gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagc                                                                                 >  1:103935‑M1/1‑82 (MQ=255)                                                                         aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttttttttgttgtatttcaatctgttcaaaagtttttcgctacgctagcagacacaaatcac                                                                        >  2:569364‑M1/1‑73 (MQ=255)                                                                          cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                       <  1:664586‑M1/149‑78 (MQ=255)                                                                                  gggttcgatttTGAATAATGCGTGATTCAGATCACACAAAACAATCAATTAGTTAACATAAATGtataagtcatacttctggtttgggtgtatttcaatctgttaagaagttgttcgctacgctagcaagcaaaaatgaaacaggaata                                                               <  2:859017‑M1/144‑86 (MQ=255)                                                                                   gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataa                                                              >  2:862480‑M1/1‑63 (MQ=255)                                                                                            ttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaataatgaaacaggaataatcgaaatgg                                                     >  1:894592‑M1/1‑54 (MQ=255)                                                                                                     gCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcg                                            <  1:361506‑M1/149‑105 (MQ=255)                                                                                                     gCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcg                                            <  1:363336‑M1/149‑105 (MQ=255)                                                                                                                aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                                 <  1:562642‑M1/149‑116 (MQ=255)                                                                                                                 tCACAAAAAACACTCAAGTAATTAACATAAAGGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagtaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaat                                <  2:181527‑M1/149‑117 (MQ=255)                                                                                                                   acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                              >  1:547753‑M1/1‑31 (MQ=255)                                                                                                                     acaAAACACTCAATTACTTAACATAAATAtataagtaatacttttgtattgggtgtatttcaatcttttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgatattgggtgttgcgcacagccaacataacc                            >  2:104893‑M1/1‑29 (MQ=255)                                                                                                                      caAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatctaaatgggatgttgcgcacagtcaaaataactc                           >  2:70960‑M1/1‑28 (MQ=255)                                                                                                                       aaaaCACTCAATTACTTAACATAAATGtataagtcatccttttgttttgagtgtatttcaatctgttaaaaagtttttcgctaagcattcaagctaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                          <  1:757160‑M1/149‑123 (MQ=255)                                                                                                                        aaaCACTCAATTACTTAACATAAATGtataagtcacacttgtgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcac                         <  1:244739‑M1/149‑124 (MQ=255)                                                                                                                         aaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacccagcgccagagaaatgaaacaggaataattgaaatgggatgttgcgcacagtcaaaataactaccc                        >  1:662492‑M1/1‑25 (MQ=255)                                                                                                                           cacTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgt                      >  1:258445‑M1/1‑23 (MQ=255)                                                                                                                              tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttcaaaagtttttcgctacgctagcaagcaaaactcaaacaggactaatccaaaagcgatgttgcgcacactcaaaataactcaccgtaaa                   >  2:31092‑M1/1‑20 (MQ=255)                                                                                                                                               aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaa  >  1:226447‑M1/1‑3 (MQ=255)                                                                                                                                                 |                                                                                                                                                   AATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913_3_KO_pgi/1978358‑1978648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑