Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 257908 258683 776 36 [1] [0] 35 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 2579071 (0.020)35 (0.730) 32/270 0.6 98.7% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_KO_pgi 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

CGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCC  >  NC_000913_3_KO_pgi/257760‑258047
                                                                                                                                                   |                                                                                                                                            
cgcgTGTGGTAAAAAGGTGCTAAAGTTTGGCAATTTGGTAAAACAGTTGCATCACAAGAGGAGATAGCAATGACGGCACCGAGTGGACACCCGAAGAGCAGATTGATCAAGTAATTTACCGCACTAGGTCCGTATATTCGTGAAGGTAa                                                                                                                                             <  2:331221‑M1/149‑2 (MQ=255)
      tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTTATCAAAAAATTTACCGCACTAGGTCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                       <  1:743248‑M1/149‑8 (MQ=255)
      acttAAAAAATTGCTAAACTTTACCAATCTGGTAAAACAGTTTCAACACAACAGAAAATAACAATAACGTTACCCACTCCACACCCGAAGAACAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAaggtaagtgcaa                                                                                                                                       <  2:81546/147‑5 (MQ=255)
            aacgttgctaAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataa                                                                                                                                 <  1:719093‑M1/145‑14 (MQ=255)
             aaTTTGTTAAATTGTGCCAATTTGGTAAAACAGTTGCATCACACCAGGAGATAGCAAAGACGTTACTGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                <  2:99653‑M1/149‑15 (MQ=255)
              aTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                                                               <  1:55511‑M1/149‑16 (MQ=255)
               tttGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcg                                                                                                                              >  1:110790‑M1/1‑133 (MQ=255)
                ttGCAAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGAAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcga                                                                                                                             <  2:97926‑M1/149‑18 (MQ=255)
                   aactgttttaccaaATTGGCAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATAATCGTGAAGGTAagtgcaaggatcagcaattc                                                                                                                          >  1:397917‑M1/6‑129 (MQ=255)
                        ttttGCCAATTGGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataagcgattcttttt                                                                                                                     <  1:565409‑M1/149‑26 (MQ=255)
                         tttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAACATTTATCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                    <  1:854885‑M1/149‑27 (MQ=255)
                            gCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgat                                                                                                                 <  1:592124‑M1/149‑30 (MQ=255)
                              cAATTTGGTAAAACAGTTGCAACACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGACTATTCGTTAAGGTAagtgcaacgataatagattcttattcgagtg                                                                                                               >  2:421376‑M1/1‑118 (MQ=255)
                                aTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtc                                                                                                             <  1:431658‑M1/149‑34 (MQ=255)
                                   tGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                          <  1:341740‑M1/149‑37 (MQ=255)
                                     ggaaaagaGTGCCAGCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCAGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggct                                                                                                        <  2:200047‑M1/147‑39 (MQ=255)
                                                 cATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                            <  1:874125‑M1/149‑51 (MQ=255)
                                                      caacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaaggtgaa                                                                                       >  2:479944‑M1/1‑94 (MQ=255)
                                                                     aTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataagcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                        >  1:257888‑M1/1‑79 (MQ=255)
                                                                         cggtaccGAGTGAACACCCGAAGAGCAGATTTATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                    <  2:549813‑M1/146‑75 (MQ=255)
                                                                                 aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                            <  1:244591‑M1/149‑83 (MQ=255)
                                                                                  gTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                           <  2:110790‑M1/149‑84 (MQ=255)
                                                                                  gTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                           <  2:291022‑M1/149‑84 (MQ=255)
                                                                                  gTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                           >  1:735486‑M1/1‑66 (MQ=255)
                                                                                      acacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgactgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggct                                                       >  2:342871‑M1/1‑62 (MQ=255)
                                                                                        acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagctaatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggcagtgcgtgaattctggggctgc                                                     >  2:498955‑M1/1‑60 (MQ=255)
                                                                                                 gCAGATTGATCAAAAAATTTACCGCACTAGGCCCTTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggag                                            <  2:521397‑M1/149‑99 (MQ=255)
                                                                                                        gATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgtttgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggttggcgctggaag                                     >  2:230705‑M1/1‑44 (MQ=255)
                                                                                                            aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                 <  1:427600‑M1/149‑110 (MQ=255)
                                                                                                              aaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatagattctttcacgattttctggctgtatgcgtcaacgtgaaaccggcaccgggagttggtggattctgggggtggtggatggagctggaagcgcagg                               >  1:528743‑M1/1‑38 (MQ=255)
                                                                                                                        gCACTAGGTCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttt                     <  2:511924‑M1/149‑122 (MQ=255)
                                                                                                                               gCCCGTATATTCGTGAGGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctac              >  1:790237‑M1/1‑21 (MQ=255)
                                                                                                                               gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttaccta               <  2:303950‑M1/148‑128 (MQ=255)
                                                                                                                               gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttaccta               >  1:303950‑M1/1‑21 (MQ=255)
                                                                                                                                   gTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgaaggaatcccgttttacctacagtt          >  1:729071‑M1/1‑17 (MQ=255)
                                                                                                                                           gTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgggagcgcaggaatcccgttttacctacagttaccagttt  >  1:499719‑M1/1‑9 (MQ=255)
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CGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCC  >  NC_000913_3_KO_pgi/257760‑258047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: