| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,757 | +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC | intergenic (‑524/‑583) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233757 | 0 (0.000) | 40 (1.160) +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC |
36/194 | 0.0 | 100% | intergenic (‑524/‑583) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233758 = | 0 (0.000) | intergenic (‑525/‑582) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
GGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCT > NC_000913_3_KO_pgi/4233611‑4233899 | actcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcaaaccgctgcctggcaggcactacgg > 2:706671‑M2/148‑149 (MQ=255) ctcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgt > 1:3318‑M2/147‑149 (MQ=255) tcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtt > 2:290509‑M2/146‑148 (MQ=255) tcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgtg > 2:111414‑M2/146‑149 (MQ=255) tcaacattacgctaacggcactaaaacaatcaaatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggaactacGgtg > 1:123557‑M2/146‑149 (MQ=255) cattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAgg < 1:341297‑M2/8‑1 (MQ=255) gctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAg > 1:788952‑M2/136‑149 (MQ=255) ggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtatttctaatgaaaaacatcaatccaacgcagaccgctccctggcaggcactacGGTGTAGGCTGGAGCTGCtt > 2:251545‑M2/130‑149 (MQ=255) actaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattcccaaaatcagaagagtattgctaaagaacaccatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGa < 1:870704‑M2/23‑1 (MQ=255) actaaaaccatcacatttttctgtgactggcgctacaatcttccaaagacacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatacaaagcagaccgctgcctggcaggcactacGGTGTAGCCTGGACCTGCTTCGa > 2:752742‑M2/127‑149 (MQ=255) ctaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGaa < 1:507993‑M2/24‑1 (MQ=255) taaaaccatcacatttttctgtgactgccgctaaccccacctatagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGTTGTAGGCTGGGGCTATTTAGAAg > 2:153181‑M2/125‑149 (MQ=255) aaactcatcacattttcatgtgcctggcgccacaatcttccaaagtaacaattctcaaaatcagaagagtattgctaatgaaccacctgaagcctacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGt < 1:302922‑M2/26‑1 (MQ=255) aaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaatcatcacaacaagaagagtattggtaatgaagaacatcatgccggcgcagtccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTGCGAAGTTc < 1:178298‑M2/28‑1 (MQ=255) atcacttttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaattagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGGAGTCTGGAGCTGCTTCGAAGTTCCtat < 2:104850‑M2/32‑1 (MQ=255) tcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCtata > 1:240365‑M2/117‑149 (MQ=255) cacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATAc < 1:326553‑M2/34‑1 (MQ=255) atttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTCTACttt > 1:422953‑M2/113‑149 (MQ=255) ttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCt < 1:517336‑M2/39‑1 (MQ=255) tttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcagtgacgtcGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTa < 1:666642‑M2/40‑1 (MQ=255) ttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTag < 2:672117‑M2/41‑1 (MQ=255) tgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAAt < 1:12056‑M2/46‑1 (MQ=255) actggcgctactatcttccaaagtcacaattctcaaaatcagaagagtattgctaacgaaaaccatcaatccaacgcagaccacggcctgccaggcactacGTTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGACAATAg < 2:184603‑M2/48‑1 (MQ=255) ctggctctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAgg < 1:443820‑M2/49‑1 (MQ=255) tggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATCGGa > 2:471216‑M2/100‑149 (MQ=255) tggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTACAGAATAGGa > 2:557340‑M2/100‑149 (MQ=255) cgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACt < 1:906968‑M2/53‑1 (MQ=255) ctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGACGTGAGCTGTTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTc < 1:128573‑M2/55‑1 (MQ=255) aatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAGCTTCGAAc > 1:123902‑M2/91‑149 (MQ=255) tcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTg > 1:601673‑M2/89‑149 (MQ=255) cttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGc < 1:111414‑M2/62‑1 (MQ=255) caaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGt > 2:217263‑M2/84‑149 (MQ=255) aaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTc > 1:712743‑M2/83‑149 (MQ=255) cacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACgg < 2:805240‑M2/72‑1 (MQ=255) acaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGa > 1:520294‑M2/77‑149 (MQ=255) aattctcaaaatcaaaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGACCTGCTTCCAAGTTCCCATACTTTCTAGAGAATAGCAACTTCGAACTGCAGGTCGACGGATc > 2:696773‑M2/75‑149 (MQ=255) aaaagcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGaa < 2:769723‑M2/82‑1 (MQ=255) aaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAat > 1:678399‑M2/67‑149 (MQ=255) gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTTGAGCTGCTTCGAAGTTCCTATACTTTCTAGATAATAGGAACTTCGAACTGCAGGTCGACGGATCcccggaatgctcga < 2:201893‑M2/89‑7 (MQ=255) aagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATccccggaatgctcgac > 1:127380‑M2/60‑142 (MQ=255) gcactacggtgtaggcTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgata > 2:263999‑M1/8‑90 (MQ=255) gcactacggtgtaggcTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgata > 1:134973‑M1/8‑90 (MQ=255) tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgata > 1:769284‑M1/4‑86 (MQ=255) tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgata < 2:769284‑M1/142‑60 (MQ=255) | GGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCT > NC_000913_3_KO_pgi/4233611‑4233899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |