Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 2,513,351 C→A L104M (CTG→ATG)  nupC → nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi2,513,3510CA95.8% 156.9 / ‑3.5 48L104M (CTG→ATG) nupCnucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (24/22);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTTAT  >  NC_000913_3_KO_pgi/2513207‑2513496
                                                                                                                                                |                                                                                                                                                 
ttCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGGATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGAt                                                                                                                                               >  1:559231/1‑149 (MQ=255)
     ggaaGGTTGCTCCGAAATGTTCTAAAACCTGGTCGGATTTGCCAATTAATGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCCTGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTa                                                                                                                                          <  2:29614/147‑1 (MQ=255)
        aGGCCTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtc                                                                                                                                       <  1:420865/149‑1 (MQ=255)
            ttCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCGCACTGCTGTTGCTCTGCAATGATCGGTATTCTCCa                                                                                                                                   <  2:664702/149‑1 (MQ=255)
            ttCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCa                                                                                                                                   <  2:620860/149‑1 (MQ=255)
               tcCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTATCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTGTATTTGCACTGACCGGTGTTCTCAAgaa                                                                                                                                >  2:448958/1‑147 (MQ=255)
                  gAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACAt                                                                                                                             >  2:587183/1‑149 (MQ=255)
                        ttCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgt                                                                                                                       <  1:587183/149‑1 (MQ=255)
                                     tCGGATTTGCCAACTAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGatc                                                                                                          >  2:182415/1‑149 (MQ=255)
                                     tCGGATTTGCCAACGAAGGGACTAACTTCGGCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGatc                                                                                                          <  1:11356/149‑1 (MQ=255)
                                       ggATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCCTTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGAATTCTCCAGCACATTCGCGTGTTGCCGGTGatcat                                                                                                        >  1:30622/1‑149 (MQ=255)
                                                 aCGAAGTGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAtt                                                                                              <  2:550228/149‑1 (MQ=255)
                                                 aCGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAtt                                                                                              >  2:326343/1‑149 (MQ=255)
                                                     aGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGtt                                                                                          <  1:376779/149‑1 (MQ=255)
                                                           tAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTACAATGATCGGTATtctc                                                                                                                                     >  1:27914/1‑100 (MQ=255)
                                                           tAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTACAATGATCGGTATtctc                                                                                                                                     <  2:27914/100‑1 (MQ=255)
                                                             aCTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCACAATCGTCTTTATATCTGCAATCATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGctc                                                                                  >  1:508807/1‑149 (MQ=255)
                                                              cTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCACGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGcgct                                                                                 >  1:139730/1‑149 (MQ=255)
                                                                     tttGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGt                                                                          <  2:494940/149‑1 (MQ=255)
                                                                            gCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGc                                                                   >  1:142019/1‑149 (MQ=255)
                                                                             cATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCa                                                                  >  1:534576/1‑149 (MQ=255)
                                                                                      tCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTcgc                                                                                                                         >  2:161244/1‑85 (MQ=255)
                                                                                      tCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTcgc                                                                                                                         <  1:161244/85‑1 (MQ=255)
                                                                                          ggCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGa                                                     <  2:534576/149‑1 (MQ=255)
                                                                                                gCATTCTTCTTCCTGAAACTGCTGTGCCCAATCGTCTTGACCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCtt                                               <  2:357171/149‑1 (MQ=255)
                                                                                                  aTTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTa                                             >  2:555819/1‑149 (MQ=255)
                                                                                                   ttcttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAACGTCACCGGCATGGGCAAACTGGAATCCTTTaa                                            >  2:371523/1‑149 (MQ=255)
                                                                                                         ttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACCGGAATCCTTTAACGCCGt                                      >  2:584674/1‑149 (MQ=255)
                                                                                                            cTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTTCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAg                                   >  2:204795/1‑149 (MQ=255)
                                                                                                            cTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                            <  1:318571/108‑1 (MQ=255)
                                                                                                            cTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                            >  2:318571/1‑108 (MQ=255)
                                                                                                            cTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAg                                   >  1:367712/1‑149 (MQ=255)
                                                                                                             tGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                            >  1:46675/1‑107 (MQ=255)
                                                                                                             tGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                            <  2:391146/107‑1 (MQ=255)
                                                                                                             tGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                            >  1:391146/1‑107 (MQ=255)
                                                                                                             tGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                            <  2:46675/107‑1 (MQ=255)
                                                                                                                 atcgCTGTGCCTAATCGTCTTTATCTCGGAAATGATCGGTATTCTCCAGCACATTCGCCTCTTGCCGGTGATCATCCGCGCAATTGTTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTccc                              <  1:385680/146‑1 (MQ=255)
                                                                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgc                                                                                                    <  1:578831/72‑1 (MQ=255)
                                                                                                                        tgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgc                                                                                                    >  2:578831/1‑72 (MQ=255)
                                                                                                                          cccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCtt                                               <  1:661995/123‑1 (MQ=255)
                                                                                                                          cccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCtt                                               >  2:661995/1‑123 (MQ=255)
                                                                                                                          cccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTgg                     <  1:182415/149‑1 (MQ=255)
                                                                                                                                  tCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGatc                                                                                                          >  1:9185/1‑56 (MQ=255)
                                                                                                                                  tCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGatc                                                                                                          <  2:9185/56‑1 (MQ=255)
                                                                                                                                    tttATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTTAGCTCCCTGATTCTGGGTCAGTCTGa           >  1:487763/1‑149 (MQ=255)
                                                                                                                                       aTCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGaaaa        >  1:84051/1‑149 (MQ=255)
                                                                                                                                            tGCAATGATCAGTAGGCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGAATCTGGGTCAGTCTGAAAACTTTa   >  2:181960/1‑149 (MQ=255)
                                                                                                                                             gCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTTAt  <  2:142019/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTTAT  >  NC_000913_3_KO_pgi/2513207‑2513496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑