| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_KO_pgi | 3,316,883 | T→G | E215A (GAA→GCA) | nusA ← | transcription termination/antitermination L factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_KO_pgi | 3,316,883 | 0 | T | G | 97.1% | 106.5 / ‑3.9 | 35 | E215A (GAA→GCA) | nusA | transcription termination/antitermination L factor |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (14/20); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01 | |||||||||||
GACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCA > NC_000913_3_KO_pgi/3316739‑3317017 | gACACCGCCTGAACACGGGCGCCACGCATACCTGCGTAAGCACGTACCTTGTCGTTTCGCTTAACGTCGGTTACCACCCCGAGTCTCGCATTAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTgg < 2:157/149‑1 (MQ=255) acCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCAc < 1:395003/149‑1 (MQ=255) acCGACTGTACACGCCCGCCCCGCATACCTACGCAACAACCTACCGGACCGCTACATAAATCACAGTCTTTCAACGGCGTTTTCGCACGAGAACCCGGCTCGCGCGCCCCTGCTTTACCTTCAATCACTTCTTCGCCGATGGCTGGCAc > 2:454684/1‑149 (MQ=255) ccGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTTTTCGCGGATTGCTGGctct > 1:384008/1‑149 (MQ=255) ccGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACt < 1:454046/149‑1 (MQ=255) cGCCTGAACACGCGCGCCACGCATACCCACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACtt < 1:482726/149‑1 (MQ=255) aaCACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTAACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTGCAATAc > 1:315132/1‑149 (MQ=255) cgcgcgCCACGCAGACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGaa < 2:502474/149‑1 (MQ=255) gcCACGCATACCTACGCAAGGACCTACCGGATCGATACGTTTCTCGTTGGTTTTCACCGCGATTTTCGCACGAGAATCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGtt < 1:456072/149‑1 (MQ=255) cATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCa < 1:578737/149‑1 (MQ=255) aTACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAg < 2:308426/149‑1 (MQ=255) aTACCTACGCAAGCACCNCNCNGNTNGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAg < 1:481927/149‑1 (MQ=255) tgccTGCGCAAGCACCTGCCGGGTCGATTCGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGc < 2:430155/147‑1 (MQ=255) tACTTACGCAAGCACCTAGCGGATCGATACGTTTATCGTGGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGc < 2:256758/149‑1 (MQ=255) aCGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTc > 2:850/1‑149 (MQ=255) tgccGGATCGTTACGGTTTTCGTTGGTTTGCACTGCGATTTTCGCACGAGAACCCGGATCGCGAGGCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAAc < 2:114453/147‑1 (MQ=255) tACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGAAACAGTTCGATCAGCATTTCCGGCTTGGAAc > 2:445421/1‑149 (MQ=255) ccGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGa > 1:524407/1‑149 (MQ=255) ggATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGt < 2:417824/149‑1 (MQ=255) gTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATAGCTGGCAGTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGt > 1:568263/1‑149 (MQ=255) gTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGAACCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTCCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGt > 1:570224/1‑149 (MQ=255) tttATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGtt > 2:155761/1‑149 (MQ=255) ggTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCAc < 2:46317/149‑1 (MQ=255) ttttCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGAGCGCCAcgc > 2:484581/1‑149 (MQ=255) cgcgATTTTCGCACGGGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCg < 2:422309/149‑1 (MQ=255) cgcgATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCg > 2:96438/1‑149 (MQ=255) cgcgATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCCCCACGCCCTTCCg > 2:34872/1‑149 (MQ=255) gaACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATagag < 1:469363/149‑1 (MQ=255) gctgctTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTc < 1:569271/149‑1 (MQ=255) ctTTAATTTCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCa < 2:524407/149‑1 (MQ=255) tttAATTTCAATCACTTCTTAGCCGATTGCTGGCACTTCAATACGGCACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGCCCGGAGCAGAGCCAGCCACGAACGCGGTCGcccg > 2:61164/1‑147 (MQ=255) tCAATCACTTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGaa < 1:445421/149‑1 (MQ=255) tCAACCACGTCTTCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGaa < 2:358645/149‑1 (MQ=255) cttcttCGCCGATTGCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCa > 1:605595/1‑149 (MQ=255) ctccgtcGCCGATTGCTGGCACTTAAAGACGGAACAGTGCGTTCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAGCGGAATAGAGCACGCCACGATCGCGGTCGCCAGGGCGGAAGTTTTCa < 2:619828/144‑1 (MQ=255) | GACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCA > NC_000913_3_KO_pgi/3316739‑3317017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |