Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 257908–258676 258683 8–776 52 [0] [1] 53 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 2579070 (0.000)49 (1.430) 41/266 0.0 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_KO_pgi 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_KO_pgi/257765‑258055
                                                                                                                                              |                                                                                                                                                    
gtgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAACAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgca                                                                                                                                                >  1:335422‑M1/1‑143 (MQ=255)
   ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaag                                                                                                                                             >  1:432210‑M1/1‑140 (MQ=255)
               tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                 >  1:612035‑M1/1‑128 (MQ=255)
                   gtctgccAATTTGGTAAAAGAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                             <  2:335422‑M1/146‑26 (MQ=255)
                    tttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                                                                            <  2:476731‑M1/149‑27 (MQ=255)
                          aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                      >  1:438774‑M1/1‑117 (MQ=255)
                                gTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggct                                                                                                                <  1:498432‑M1/149‑39 (MQ=255)
                                             aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcagagataatcgcttctttttcgattgtctggctgtatgcgtaaacg                                                                                                   >  1:113318‑M1/1‑98 (MQ=255)
                                             aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacg                                                                                                   >  2:170687‑M1/1‑98 (MQ=255)
                                             aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTAATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtgtgcgtcaacg                                                                                                   >  2:2583‑M1/1‑98 (MQ=255)
                                                acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                    >  2:555929‑M1/1‑95 (MQ=255)
                                                acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                    <  1:555929‑M1/145‑51 (MQ=255)
                                                  aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                        <  1:121985‑M1/107‑15 (MQ=255)
                                                  aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                        >  2:121985‑M1/1‑93 (MQ=255)
                                                        agaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAACCTGGACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggca                                                                                        >  1:289383‑M1/1‑87 (MQ=255)
                                                          aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttttgattgtctggctgtatgcgtcaacgtgaaaccggcacc                                                                                      >  2:433808‑M1/1‑85 (MQ=255)
                                                              cAATGACGTTACCGAGTGGACGCCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgaacattcttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                                  <  2:271623‑M1/149‑69 (MQ=255)
                                                                aTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                                <  1:197240‑M1/149‑71 (MQ=255)
                                                                           gAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgggagtgcatgaattct                                                                     >  1:328752‑M1/1‑68 (MQ=255)
                                                                            aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgagtgaattcgg                                                                    >  1:238214‑M1/1‑67 (MQ=255)
                                                                              tGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACGAGGCGCGTATATTCGGGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgtgaaacaggaaccgggaatgagtgaattctggg                                                                  >  1:130879‑M1/1‑65 (MQ=255)
                                                                               ggACACCAGAAGAGAAGATTGAGCAAAAAATTTACCGCAGTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggg                                                                 <  2:127896‑M1/149‑86 (MQ=255)
                                                                                  cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctg                                                              >  2:631712‑M1/1‑61 (MQ=255)
                                                                                     ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctagctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                           >  2:465033‑M1/1‑58 (MQ=255)
                                                                                     ccGAAGAGCAGATTGATCAAAAAATTTAAAGCAATCCTTCTTTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                           >  1:630750‑M1/1‑58 (MQ=255)
                                                                                      cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgg                                                          >  1:540945‑M1/1‑57 (MQ=255)
                                                                                       gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTCTATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                         <  2:612035‑M1/149‑94 (MQ=255)
                                                                                           agCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatgga                                                     >  2:385704‑M1/1‑52 (MQ=255)
                                                                                            gCAGATTGATCAAAAAATTTACCGCACTAGGACCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtat                                                                                                            <  2:466476‑M1/93‑43 (MQ=255)
                                                                                            gCAGATTGATCAAAAAATTTACCGCACTAGGACCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtat                                                                                                            >  1:466476‑M1/1‑51 (MQ=255)
                                                                                                   ggtcaAAAAATTTACCGCACTAGGCCCGTATATGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaag                                             <  2:486975‑M1/147‑106 (MQ=255)
                                                                                                    aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaatcctggggctgggggatggcgctggaagc                                            >  2:224292‑M1/1‑43 (MQ=255)
                                                                                                    aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                    >  1:560748‑M1/1‑43 (MQ=255)
                                                                                                    aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                    <  2:560748‑M1/93‑51 (MQ=255)
                                                                                                     tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                           >  1:594913‑M1/1‑42 (MQ=255)
                                                                                                       aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGACGGTCagtgtattgttaatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         <  1:15858‑M1/149‑110 (MQ=255)
                                                                                                       aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         <  1:66631‑M1/149‑110 (MQ=255)
                                                                                                       aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttatcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggcgctggaagcgca                                         >  1:560643‑M1/1‑40 (MQ=255)
                                                                                                       aaaaaaTTTACCGCACTAGGCCCGTATATTAGTGACGGTAactggaatgataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         <  1:15943‑M1/149‑110 (MQ=255)
                                                                                                        aaaaaTTTACCGCACTGGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                        <  2:180516‑M1/149‑111 (MQ=255)
                                                                                                                  cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtt                              <  1:329014‑M1/149‑121 (MQ=255)
                                                                                                                  cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggt                                                            <  1:621377‑M1/119‑91 (MQ=255)
                                                                                                                  cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggt                                                            >  2:621377‑M1/1‑29 (MQ=255)
                                                                                                                     aCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagggcgtgaattctggggctgggggatggagctggaagcccaggactcccgtctaa                           >  2:92432‑M1/1‑26 (MQ=255)
                                                                                                                      cTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgcattgagctggaagcgcaggaatcccgttttac                          <  1:631712‑M1/149‑125 (MQ=255)
                                                                                                                             cGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcccaggaatcccgttttaccgacatt                   <  2:432210‑M1/149‑132 (MQ=255)
                                                                                                                               tataTTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtcttgctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctagtggatg                                                       <  1:57672‑M1/111‑96 (MQ=255)
                                                                                                                               tataTTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctagtggatg                                                       >  2:57672‑M1/1‑16 (MQ=255)
                                                                                                                                     ggtGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtt           <  2:391391‑M1/148‑140 (MQ=255)
                                                                                                                                        gAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttgg        <  1:8975‑M1/149‑143 (MQ=255)
                                                                                                                                            gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctg    >  2:439924‑M1/1‑3 (MQ=255)
                                                                                                                                              aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  1:170687‑M1/149‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913_3_KO_pgi/257765‑258055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.