Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913_3_KO_pgi | 1,166,727 | G→C | E215Q (GAA→CAA) | ndh → | respiratory NADH dehydrogenase 2/cupric reductase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913_3_KO_pgi | 1,166,727 | 0 | G | C | 99.0% | 329.8 / ‑3.6 | 95 | E215Q (GAA→CAA) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (50/44); total (50/45) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 |
CGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCG > NC_000913_3_KO_pgi/1166582‑1166871 | cgcgAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGc < 3:390722/150‑1 (MQ=255) gAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCggg > 1:354430/1‑150 (MQ=255) gAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCggg > 1:912042/1‑150 (MQ=255) aaTGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGga < 4:79119/150‑1 (MQ=255) aaTGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCGGCGCTGAATGGCACAACGCGATCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGga > 4:513851/1‑150 (MQ=255) gCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAAc > 1:159176/1‑150 (MQ=255) gCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAAc < 2:884057/150‑1 (MQ=255) cAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACg > 4:549461/1‑150 (MQ=255) cAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACg > 2:123854/1‑150 (MQ=255) cAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCGGCGCTGGATGGAACAACGCGATCAAGAAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACg > 4:29756/1‑150 (MQ=255) cAAAGTGAACATGGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTCCGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGCACAAGCGGgagacgg > 2:123809/1‑147 (MQ=255) tGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTAttt < 4:172143/150‑1 (MQ=255) tGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCGCCGCTGAATTGCACAACGCGGGCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCAGATACAAGCGGGAGAACGTAgtt > 2:38125/1‑150 (MQ=255) tGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAtttt > 3:364309/1‑144 (MQ=255) tGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAtttt < 4:364309/144‑1 (MQ=255) gATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGtt > 1:102713/1‑150 (MQ=255) gTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTAccc > 1:689791/1‑149 (MQ=255) gcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTa > 3:548784/1‑150 (MQ=255) cggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAt < 4:252528/150‑1 (MQ=255) ggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAtc > 1:283729/1‑150 (MQ=255) ggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAtc > 4:135509/1‑150 (MQ=255) gACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCg > 4:536023/1‑136 (MQ=255) gACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCg < 3:536023/136‑1 (MQ=255) gggTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCtgctgc > 3:475263/1‑150 (MQ=255) gtgtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCgg > 3:369344/1‑150 (MQ=255) gtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGcc < 1:545661/150‑1 (MQ=255) tAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGccc > 4:379304/1‑150 (MQ=255) ctcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGa < 3:288983/150‑1 (MQ=255) tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGACCGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAg < 4:25390/150‑1 (MQ=255) cGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCt > 3:108496/1‑150 (MQ=255) cGCTGAATTCCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCt > 1:623170/1‑150 (MQ=255) tGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAAc > 1:707188/1‑150 (MQ=255) aaTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGa > 1:583995/1‑150 (MQ=255) aaTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGa > 4:150350/1‑150 (MQ=255) tGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAAc > 3:56556/1‑150 (MQ=255) tGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCAGATACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCCGCCCACAACGAGCTCACGGAAc > 2:35255/1‑150 (MQ=255) gCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACt > 1:882971/1‑150 (MQ=255) cacaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACtt > 3:440939/1‑150 (MQ=255) acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTg > 4:154286/1‑150 (MQ=255) acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTg > 3:490766/1‑150 (MQ=255) acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTg > 3:302152/1‑150 (MQ=255) caACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTgg < 2:882971/150‑1 (MQ=255) aaCTCGGACAAGCAACTGCACAGCTACGGTTACAAAGACCTGACCAAGGAAGCCCTGCAGGTGTCTCTGGCACAAGCGCGAGAACGGACTTTGCCTGCCTTACCGCCACGCACCTCTTCTGCAGCGCACAACGAGCCAACGAAACTTgac > 2:902936/1‑148 (MQ=255) aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACt < 1:107696/146‑1 (MQ=255) aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACt > 2:107696/1‑146 (MQ=255) aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTg > 3:469243/1‑148 (MQ=255) aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTg < 4:469243/148‑1 (MQ=255) gcgGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGtt > 2:845181/1‑150 (MQ=255) ggTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcg > 2:606195/1‑150 (MQ=255) gTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcgc < 3:483210/150‑1 (MQ=255) gTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcgc < 4:475263/150‑1 (MQ=255) aaGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTg > 3:371610/1‑150 (MQ=255) aGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGc < 1:771434/150‑1 (MQ=255) gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCt < 1:104049/150‑1 (MQ=255) tGCACAGCTACGGTTACAAAGGCCTGACCAACTAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGc < 3:316205/150‑1 (MQ=255) gCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCa < 1:793641/150‑1 (MQ=255) cTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGAAGccatcgag > 2:265366/1‑143 (MQ=255) tACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATg < 3:302543/150‑1 (MQ=255) tACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATg < 2:159176/150‑1 (MQ=255) cGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGTAAGTATTTTGCGTGCGATACGCTCACTTTTGTGGGCTACGGCCGACAGCTAGGTAGCGAAACTTGGCGTTCGCGTGCTGACGCAAACCATAGt > 1:781993/1‑150 (MQ=255) cGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGt < 3:130004/150‑1 (MQ=255) gTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGAACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCa > 1:824475/1‑150 (MQ=255) ttACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCAc > 4:311092/1‑150 (MQ=255) ttACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTGACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCAc < 4:23447/150‑1 (MQ=255) aCAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa < 1:296500/100‑1 (MQ=255) aCAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa > 2:296500/1‑100 (MQ=255) aaaGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGt > 1:396195/1‑150 (MQ=255) aaaGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGt < 4:295401/150‑1 (MQ=255) ccTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtga < 3:311092/150‑1 (MQ=255) ccTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtga > 3:365170/1‑150 (MQ=255) tGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtgatg > 2:793724/1‑150 (MQ=255) tGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtgatg > 2:793650/1‑150 (MQ=255) aCCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGaa < 4:490766/150‑1 (MQ=255) aaCGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggc < 1:887810/150‑1 (MQ=255) aCGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcg < 4:108496/150‑1 (MQ=255) gAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcggc < 3:135509/150‑1 (MQ=255) aGCCCTGAACGTAGCGCTGGTACGAGCGGGAGAACGGATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCt < 2:689791/150‑1 (MQ=255) aGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCt < 3:307165/150‑1 (MQ=255) ccTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGca < 4:548784/150‑1 (MQ=255) ccTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGca > 2:446117/1‑150 (MQ=255) gAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGGGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCacac < 3:130135/150‑1 (MQ=255) gAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCacac < 4:365170/150‑1 (MQ=255) cGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaa < 2:897838/150‑1 (MQ=255) gTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaaa < 3:379304/150‑1 (MQ=255) gTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaaa < 3:536353/150‑1 (MQ=255) aaCGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGa < 3:150350/150‑1 (MQ=255) cGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATg < 1:793724/150‑1 (MQ=255) cGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATg < 1:793650/150‑1 (MQ=255) gCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATgg < 2:396195/150‑1 (MQ=255) actcgTACAAGCGGGAGAACGAATATTACCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACAAAACTAAGCGTTCGAATGATGACGACAACCATGGACACCAGTGCAGATGAAGGCGGCCTGCACACTAACGATgg < 2:782413/146‑1 (MQ=255) cTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGc > 3:580613/1‑150 (MQ=255) cTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGc < 2:354430/150‑1 (MQ=255) tGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACATCGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCg < 1:85001/150‑1 (MQ=255) tGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCg > 2:766682/1‑150 (MQ=255) gTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAAc > 2:1087/1‑76 (MQ=255) | CGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCG > NC_000913_3_KO_pgi/1166582‑1166871 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |