Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	3,397,657	Δ9 bp	coding (131‑139/1470 nt)	rng ←	ribonuclease G

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	3397657	3397665	9	75 [0]	[0] 75	rng	ribonuclease G

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 3397656	0 (0.000)	73 (0.880)	58/264	0.5	100%	coding (140/1470 nt)	rng	ribonuclease G
	?	NC_000913_3_KO_pgi	3397666 =	0 (0.000)					coding (130/1470 nt)	rng	ribonuclease G

CAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCC  >  NC_000913_3_KO_pgi/3397508‑3397804                                                                                                                                                     |                                                                                                                                                     cAGTTCCGAGATGTCACGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACc                                                                                                                                                     >  1:289173‑M1/1‑149 (MQ=255)  aGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACcc                                                                                                                                                    >  1:480140‑M1/1‑148 (MQ=255)   gTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACcct                                                                                                                                                   >  1:725785‑M1/1‑147 (MQ=255)      ccGAGATGGTGCGCAGCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgt                                                                                                                                                <  2:480140‑M1/150‑7 (MQ=255)                  gcACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGACACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgccta                                                                                                                                    <  1:28618‑M1/150‑19 (MQ=255)                    aCCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctact                                                                                                                                  >  1:37511‑M1/1‑130 (MQ=255)                     ccGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctacta                                                                                                                                 >  2:445817‑M1/1‑129 (MQ=255)                        tGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTGCAAAAGCCGCCTGCATACCCGGCAGTACACGACccttgtagatattgcctactattc                                                                                                                              >  2:448753‑M1/1‑126 (MQ=255)                        tGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattc                                                                                                                              >  3:465122‑M1/1‑126 (MQ=255)                          aaTTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccg                                                                                                                            >  2:367035‑M1/1‑124 (MQ=255)                           aTTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgc                                                                                                                           <  1:819266‑M1/150‑28 (MQ=255)                             tGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgt                                                                                                                         <  1:691601‑M1/150‑30 (MQ=255)                               cTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcg                                                                                                                       >  2:774660‑M1/1‑119 (MQ=255)                                           aCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttc                                                                                                           >  3:556123‑M1/1‑107 (MQ=255)                                           aCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttc                                                                                                           >  1:515313‑M1/1‑107 (MQ=255)                                            cccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttca                                                                                                          >  3:464500‑M1/1‑106 (MQ=255)                                            cccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttca                                                                                                          >  1:169877‑M1/1‑106 (MQ=255)                                             ccGCCCCACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCAAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaa                                                                                                         <  2:732590‑M1/150‑46 (MQ=255)                                             ccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaa                                                                                                         >  1:630960‑M1/1‑105 (MQ=255)                                             ccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaa                                                                                                         >  4:542820‑M1/1‑105 (MQ=255)                                             ccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaa                                                                                                         <  3:413310‑M1/150‑46 (MQ=255)                                             ccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaa                                                                                                         >  2:235928‑M1/1‑105 (MQ=255)                                               gCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaata                                                                                                       >  1:451906‑M1/1‑103 (MQ=255)                                                    acaTTGGGTGTGCGGCATGGTGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaat                                                                                                  <  2:462733‑M1/150‑53 (MQ=255)                                                          ggTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctg                                                                                            >  4:337865‑M1/1‑92 (MQ=255)                                                            tgtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgca                                                                                          <  3:389957‑M1/150‑61 (MQ=255)                                                            tgtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgca                                                                                          <  3:278581‑M1/150‑61 (MQ=255)                                                                cGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgccccacgttcaatatgaatttcctgcagaat                                                                                      >  2:895733‑M1/1‑86 (MQ=255)                                                                  gCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaatac                                                                                    >  4:86828‑M1/1‑84 (MQ=255)                                                                     tgatgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgc                                                                                 >  2:696749‑M1/1‑81 (MQ=255)                                                                     tgatgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgc                                                                                 <  2:353253‑M1/150‑70 (MQ=255)                                                                          tCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatca                                                                            >  1:58814‑M1/1‑76 (MQ=255)                                                                          tCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatca                                                                            >  4:548827‑M1/1‑76 (MQ=255)                                                                             gATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaata                                                                         <  1:883762‑M1/150‑78 (MQ=255)                                                                               tGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatata                                                                       <  2:160154‑M1/150‑80 (MQ=255)                                                                                gCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatac                                                                      <  3:557093‑M1/150‑81 (MQ=255)                                                                                  aTGAAGAGACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgc                                                                    >  1:321477‑M1/1‑68 (MQ=255)                                                                                  aTGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtaaatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgccgcataccgccatcaatatacgc                                                                    >  4:171888‑M1/1‑68 (MQ=255)                                                                                   tGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgcc                                                                   <  1:75785‑M1/150‑84 (MQ=255)                                                                                     aagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgccccacgttcaatatgaatttcctgcagaataccgccatcaatatacgccac                                                                 >  2:524654‑M1/1‑65 (MQ=255)                                                                                       gaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacg                                                                                                              <  1:453764‑M1/103‑41 (MQ=255)                                                                                       gaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacg                                                                                                              >  2:453764‑M1/1‑63 (MQ=255)                                                                                          aCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcg                                                            >  4:147102‑M1/1‑60 (MQ=255)                                                                                               gCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaat                                                                                                        <  1:208131‑M1/101‑47 (MQ=255)                                                                                               gCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaat                                                                                                        >  2:208131‑M1/1‑55 (MQ=255)                                                                                                    aTCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaagg                                                  <  2:21467‑M1/150‑101 (MQ=255)                                                                                                    aTCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaagg                                                  <  1:445817‑M1/150‑101 (MQ=255)                                                                                                      ccAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcg                                                >  2:695275‑M1/1‑48 (MQ=255)                                                                                                       cAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgt                                               >  3:418656‑M1/1‑47 (MQ=255)                                                                                                          cccAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttac                                            <  4:144819‑M1/150‑107 (MQ=255)                                                                                                               tatCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgttta                                       <  3:337865‑M1/150‑112 (MQ=255)                                                                                                                atCTACAAAAGCCACCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttac                                      <  2:811274‑M1/150‑113 (MQ=255)                                                                                                                 tCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcactatacgccactcgcgtttccgaaggcgttacgtttact                                     >  3:36871‑M1/1‑37 (MQ=255)                                                                                                                 tCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttact                                     >  1:21640‑M1/1‑37 (MQ=255)                                                                                                                    aCAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaac                                  >  2:325371‑M1/1‑34 (MQ=255)                                                                                                                     cAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaaca                                 <  1:717060‑M1/150‑118 (MQ=255)                                                                                                                      aaaaGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaa                                >  2:208055‑M1/1‑32 (MQ=255)                                                                                                                       aaaGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgttt                                        >  4:116296‑M1/1‑31 (MQ=255)                                                                                                                       aaaGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgttt                                        <  3:116296‑M1/141‑111 (MQ=255)                                                                                                                         aGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattc                             >  4:471555‑M1/1‑29 (MQ=255)                                                                                                                             gccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagcc                         <  3:93899‑M1/150‑126 (MQ=255)                                                                                                                             gccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagcc                         <  4:465122‑M1/150‑126 (MQ=255)                                                                                                                              ccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgttt                                        <  1:115928‑M1/134‑111 (MQ=255)                                                                                                                              ccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgttt                                        >  2:115928‑M1/1‑24 (MQ=255)                                                                                                                                 gCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtca                     <  1:288213‑M1/150‑130 (MQ=255)                                                                                                                                  cATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcat                    <  3:548827‑M1/150‑131 (MQ=255)                                                                                                                                   aTACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatg                   >  1:197396‑M1/1‑19 (MQ=255)                                                                                                                                     aCCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtt                 >  4:57287‑M1/1‑17 (MQ=255)                                                                                                                                     aCCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacggttactaacaattcagccgtcatgtt                 >  2:191162‑M1/1‑17 (MQ=255)                                                                                                                                     aCCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaattgcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtt                 >  2:492196‑M1/1‑17 (MQ=255)                                                                                                                                      cccGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgttt                >  4:152839‑M1/1‑16 (MQ=255)                                                                                                                                          gAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatcc            <  2:553520‑M1/150‑139 (MQ=255)                                                                                                                                           aaGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatccc           <  2:289173‑M1/150‑140 (MQ=255)                                                                                                                                                   gACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatcccttttctca   <  1:208055‑M1/150‑148 (MQ=255)                                                                                                                                                    aCccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatcccttttctcac  <  2:169877‑M1/150‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCC  >  NC_000913_3_KO_pgi/3397508‑3397804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑