BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	2,664,591	C→A	P68T (CCT→ACT)	yfhR →	putative S9 family prolyl oligopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	2,664,591	0	C	A	100.0%	587.9 / NA	153	P68T (CCT→ACT)	yfhR	putative S9 family prolyl oligopeptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (86/67); total (86/67)

Only 100 of 153 total aligned reads displayed.

GTTGCCTTCTGCGTTTATTTAGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCCCTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATCGCGGGTT  >  NC_000913_3_KO_pgi/2664444‑2664736

gttgCCTTCTGCGTTTATTTAGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCAct                                                                                                                                                 <  1:534337/150‑1 (MQ=255)

    ccTTCTGCGTTTATTTAGTGCCGCGCGTTGTCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCActtctt                                                                                                                                             <  1:1089926/150‑1 (MQ=255)

     cTTCTGCGTTTATTTAGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACttcttc                                                                                                                                            <  2:1388590/150‑1 (MQ=255)

     cTTCTGCGTTTATTTAGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACttcttc                                                                                                                                            >  1:1492507/1‑150 (MQ=255)

         tGCGTTTATTTAGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACg                                                                                                                                        >  4:82764/1‑150 (MQ=255)

           cGTTTATTTAGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACggg                                                                                                                                      >  2:1560951/1‑150 (MQ=255)

           cGTTTATTTAGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACggg                                                                                                                                      >  1:264427/1‑150 (MQ=255)

             tttatttaGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGcc                                                                                                                                    <  3:50143/150‑1 (MQ=255)

             tctatttaGTGCCGCGCGTTGCCATCACCTTCTTCTATTATCCCGACGATAAACTCTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAACGACGGTACTCGTATGCAAGGCTGGATTATCACTTCTTCGACGGGcc                                                                                                                                    >  2:356586/3‑150 (MQ=255)

               tatttaGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCct                                                                                                                                  <  3:726704/150‑1 (MQ=255)

                atttaGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGa                                                                                                                                          <  1:1170763/141‑1 (MQ=255)

                atttaGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGa                                                                                                                                          >  2:1170763/1‑141 (MQ=255)

                atttaGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCctg                                                                                                                                 <  2:505115/150‑1 (MQ=255)

                        ccGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAAc                                                                                                                         <  2:692915/150‑1 (MQ=255)

                           cgcgTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGcc                                                                                                                      <  3:477778/150‑1 (MQ=255)

                              gTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATc                                                                                                                   <  1:10567/150‑1 (MQ=255)

                               ttGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCg                                                                                                                  <  2:1074222/150‑1 (MQ=255)

                                    aTCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAAcc                                                                                                             >  4:120321/1‑150 (MQ=255)

                                    aTCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAAcc                                                                                                             >  3:959105/1‑150 (MQ=255)

                                     tCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGATTCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACca                                                                                                            >  2:638296/1‑150 (MQ=255)

                                     tCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACca                                                                                                            >  2:1127866/1‑150 (MQ=255)

                                       aaCTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACcatc                                                                                                          >  3:450182/1‑150 (MQ=255)

                                          ttcttcTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCAtcatt                                                                                                       >  2:1496821/1‑150 (MQ=255)

                                           tcttctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCAtcattc                                                                                                      <  2:1246712/150‑1 (MQ=255)

                                           tcttctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCAtcattc                                                                                                      <  4:450182/150‑1 (MQ=255)

                                            cttctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCAtcattca                                                                                                     >  4:6091/1‑150 (MQ=255)

                                             ttctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCAtcattcat                                                                                                    >  2:380319/1‑150 (MQ=255)

                                             ttctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCAtcattcat                                                                                                    <  1:1440349/150‑1 (MQ=255)

                                             ttctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCAtcattcat                                                                                                    >  4:353047/1‑150 (MQ=255)

                                             ttctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCAgcattcat                                                                                                    >  3:614903/1‑150 (MQ=255)

                                              tctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATg                                                                                                   <  1:1641263/150‑1 (MQ=255)

                                              tctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATg                                                                                                   >  3:523820/1‑150 (MQ=255)

                                              tctATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATg                                                                                                   >  1:47065/1‑150 (MQ=255)

                                                tattatCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCt                                                                                                 >  2:56669/1‑150 (MQ=255)

                                                         gacgaTAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAAt                                                                                        >  3:172120/1‑150 (MQ=255)

                                                              tAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGCAGGTACTCGTGTGCAAAGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCCACCATCATTCATGCTCACGGCCATGCCgg                                                                                   >  4:469668/1‑150 (MQ=255)

                                                              tAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCgg                                                                                   >  3:373004/1‑150 (MQ=255)

                                                               aaaaTTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGa                                                                                  >  1:905891/1‑150 (MQ=255)

                                                               aaaaTTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGa                                                                                  >  1:1198601/1‑150 (MQ=255)

                                                                aaaTTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGaa                                                                                 >  4:521273/1‑150 (MQ=255)

                                                                 aaTTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGaaa                                                                                <  2:1407420/150‑1 (MQ=255)

                                                                 aaTTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGaaa                                                                                >  1:624158/1‑150 (MQ=255)

                                                                 aaTTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGaaa                                                                                <  1:608779/150‑1 (MQ=255)

                                                                  aTTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAat                                                                               >  4:745201/1‑150 (MQ=255)

                                                                  aTTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAat                                                                               <  4:972679/150‑1 (MQ=255)

                                                                   tttACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAata                                                                              >  2:1692839/1‑150 (MQ=255)

                                                                    ttACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAatat                                                                             <  2:614204/150‑1 (MQ=255)

                                                                    ttACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGATTACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAatat                                                                             <  1:1655395/150‑1 (MQ=255)

                                                                     tACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATg                                                                            >  1:362287/1‑150 (MQ=255)

                                                                      aCGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGt                                                                           <  1:345897/150‑1 (MQ=255)

                                                                         gTCCCGATCCCAGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCg                                                                        >  4:883806/1‑150 (MQ=255)

                                                                             cGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCa                                                                    >  1:1168256/1‑150 (MQ=255)

                                                                              gATCCATGGACGGCGGAATCCGTAGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTATTCAACGGGGGCTGCTGCCCACGCCATCGCACCCATCATTCATGCTCACGGCAATGCCGGACATATGTCCCCCCAc                                                                   >  2:920628/1‑150 (MQ=255)

                                                                               aTCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACt                                                                  >  1:521999/1‑150 (MQ=255)

                                                                                   cTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGcc                                                              >  2:1551185/1‑150 (MQ=255)

                                                                                   cTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGcc                                                              >  1:668206/1‑150 (MQ=255)

                                                                                       tCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTg                                                          <  1:1632814/150‑1 (MQ=255)

                                                                                           cggAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCa                                                      >  2:1177640/1‑150 (MQ=255)

                                                                                              aaTCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGtt                                                   <  4:748279/150‑1 (MQ=255)

                                                                                               aTCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTg                                                  <  2:264427/150‑1 (MQ=255)

                                                                                               aTCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTg                                                  <  3:745201/150‑1 (MQ=255)

                                                                                                tCCGTCGAATTTACGGCTAGGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTgg                                                 <  4:318529/150‑1 (MQ=255)

                                                                                                tCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTgg                                                 >  3:902277/1‑150 (MQ=255)

                                                                                                   gTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTa                                              >  3:304706/1‑150 (MQ=255)

                                                                                                     cGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTAcc                                            >  4:957792/1‑150 (MQ=255)

                                                                                                      gAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTAccc                                           <  1:1260611/150‑1 (MQ=255)

                                                                                                         tttACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGtt                                                   >  2:751652/1‑139 (MQ=255)

                                                                                                            aCGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCg                                                             <  1:462037/126‑1 (MQ=255)

                                                                                                            aCGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCg                                                             >  2:462037/1‑126 (MQ=255)

                                                                                                              ggCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTaa                                   >  2:854780/1‑150 (MQ=255)

                                                                                                              ggCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTaa                                   >  1:695353/1‑150 (MQ=255)

                                                                                                              ggCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTaa                                   >  4:3789/1‑150 (MQ=255)

                                                                                                                cTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAAtt                                 >  1:724986/1‑150 (MQ=255)

                                                                                                                 tAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAAttt                                >  1:726481/1‑150 (MQ=255)

                                                                                                                        ggTACGCGTCTGCAAGGCTGTTTTATCACTTCTTCGACGGTCCCTGCTGACAACGCCATCGCAACCATCATTCATGCGCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGtt                         <  3:469668/150‑1 (MQ=255)

                                                                                                                         gTACTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGttt                        <  4:959105/150‑1 (MQ=255)

                                                                                                                           aCTCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGttttt                      <  2:1492507/150‑1 (MQ=255)

                                                                                                                             tCGTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGATTTTAt                    >  1:1625045/1‑150 (MQ=255)

                                                                                                                               gTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGt                  >  1:596492/1‑150 (MQ=255)

                                                                                                                               gTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGt                  <  3:690535/150‑1 (MQ=255)

                                                                                                                               gTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGt                  <  3:14660/150‑1 (MQ=255)

                                                                                                                               gTCTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAACTTCAACGTTTTTATGt                  <  3:826132/150‑1 (MQ=255)

                                                                                                                                 cTGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGttt                <  2:31415/150‑1 (MQ=255)

                                                                                                                                  tGCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCACCGTTTTTATGTTTg               >  4:559714/1‑150 (MQ=255)

                                                                                                                                   gCAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGa              >  1:1676145/1‑150 (MQ=255)

                                                                                                                                    cAAGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGc                                                                                                                 <  1:1198636/50‑1 (MQ=255)

                                                                                                                                     aaGGCTGGTTTATCACTTCTTTTTCTTTTTCTGCTGACAACGCCATCGCAACCATCATTCATGATCACGGCAATGCCGGAAATATGTCCGCGCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGAtt            >  4:788264/1‑150 (MQ=255)

                                                                                                                                     aaGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGAtt            >  3:436982/1‑150 (MQ=255)

                                                                                                                                     aaGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGAtt            >  3:570866/1‑150 (MQ=255)

                                                                                                                                     aaGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAACGCCGGAAATATGTCCGCTCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGAtt            >  4:788363/1‑150 (MQ=255)

                                                                                                                                      aGGCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTa           <  1:1678099/150‑1 (MQ=255)

                                                                                                                                        gCTGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATc         >  4:144904/1‑150 (MQ=255)

                                                                                                                                          tGGTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATcgc       >  2:1260099/1‑150 (MQ=255)

                                                                                                                                           ggTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATcgcg      <  2:1676145/150‑1 (MQ=255)

                                                                                                                                            gTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATCGCgg     >  3:120044/1‑150 (MQ=255)

                                                                                                                                            gTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATCGCgg     <  1:680821/150‑1 (MQ=255)

                                                                                                                                            gTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCGGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATCGCgg     <  3:984433/150‑1 (MQ=255)

                                                                                                                                            gTTTATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGAAACCGGCAAAGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATCGCgg     <  1:1340257/150‑1 (MQ=255)

                                                                                                                                             tttATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTg                                                  >  2:718575/1‑104 (MQ=255)

                                                                                                                                               tATCACTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATCGCGGGtt  <  4:304706/150‑1 (MQ=255)

GTTGCCTTCTGCGTTTATTTAGTGCCGCGCGTTGCCATCAACTTCTTCTATTATCCCGACGATAAAATTTACGGTCCCGATCCCTGGTCGGCGGAATCCGTCGAATTTACGGCTAAGGACGGTACTCGTCTGCAAGGCTGGTTTATCCCTTCTTCGACGGGCCCTGCTGACAACGCCATCGCAACCATCATTCATGCTCACGGCAATGCCGGAAATATGTCCGCCCACTGGCCGCTGGTCAGTTGGTTACCCGAGCGTAATTTCAACGTTTTTATGTTTGATTATCGCGGGTT  >  NC_000913_3_KO_pgi/2664444‑2664736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`