BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	1,399,451	T→G	S26R (AGC→CGC)	fnr ←	oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	1,399,451	0	T	G	100.0%	503.3 / NA	138	S26R (AGC→CGC)	fnr	oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (77/61); total (77/61)

Only 100 of 139 total aligned reads displayed.

CGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCA  >  NC_000913_3_KO_pgi/1399307‑1399596

cGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGAtgc                                                                                                                                              >  4:768494/1‑150 (MQ=255)

  gAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGAtgctg                                                                                                                                            >  1:756631/1‑150 (MQ=255)

  gAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGAtgctg                                                                                                                                            <  1:1064477/150‑1 (MQ=255)

  gAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGAtgctg                                                                                                                                            <  2:1391174/150‑1 (MQ=255)

  gAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGAtgctg                                                                                                                                            >  3:923192/1‑150 (MQ=255)

         tGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCt                                                                                                                                     <  2:201659/150‑1 (MQ=255)

           gCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTgg                                                                                                                                   <  2:719398/150‑1 (MQ=255)

               aaaGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAAt                                                                                                                               <  4:706176/150‑1 (MQ=255)

               aaaGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAAt                                                                                                                               <  1:525412/150‑1 (MQ=255)

                aaGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATg                                                                                                                              <  2:1353082/150‑1 (MQ=255)

                 aGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATgg                                                                                                                             >  2:474699/1‑150 (MQ=255)

                 aGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATgg                                                                                                                             <  2:756631/150‑1 (MQ=255)

                     aTTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAg                                                                                                                         <  3:615197/150‑1 (MQ=255)

                     aTTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAg                                                                                                                         <  3:615163/150‑1 (MQ=255)

                       ttAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGca                                                                                                                       >  3:762595/1‑150 (MQ=255)

                         aaGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGcaca                                                                                                                     >  4:668437/1‑150 (MQ=255)

                           gTTCATCACCAGCCTTAAGCAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAAc                                                                                                                   <  1:1368701/150‑1 (MQ=255)

                              catcaCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGc                                                                                                                >  3:951631/1‑150 (MQ=255)

                               atcaCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGcc                                                                                                               <  1:1400583/150‑1 (MQ=255)

                               atcaCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGcc                                                                                                               >  3:399537/1‑150 (MQ=255)

                                  aCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCCAGCTCATGTTCGTTGAGAGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGa                                                                                                            >  3:622306/1‑150 (MQ=255)

                                    cAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACt                                                                                                          >  1:1032319/1‑150 (MQ=255)

                                       ccTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGaa                                                                                                       >  1:827280/1‑150 (MQ=255)

                                             aCAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGcc                                                                                                 <  4:662608/150‑1 (MQ=255)

                                             aCAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGcc                                                                                                 <  4:845306/150‑1 (MQ=255)

                                              cAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATAATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCg                                                                                                >  3:909621/1‑150 (MQ=255)

                                                 cGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGtat                                                                                             <  3:585344/150‑1 (MQ=255)

                                                    cTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAAt                                                                                          >  2:464663/1‑150 (MQ=255)

                                                     tGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATgcg                                                                                                   >  1:1039369/1‑140 (MQ=255)

                                                     tGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATgcg                                                                                                   <  2:1039369/140‑1 (MQ=255)

                                                         cTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCt                                                                                     >  2:1555763/1‑150 (MQ=255)

                                                         cTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCt                                                                                     >  4:523496/1‑150 (MQ=255)

                                                               gAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCg                                                                               >  1:469345/1‑150 (MQ=255)

                                                                 aTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCGATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCggg                                                                             <  2:917923/150‑1 (MQ=255)

                                                                     gCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCa                                                                         >  1:390897/1‑150 (MQ=255)

                                                                     gCTTCTGCCGCTCAATGATATTATCAAGCGGATCAAGCACATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGTGGATGCTGCAAACCTGGCAATGGATAGCACAACCGCCAGACTTAATGAGCCGTATAATTCGCTTTTCCGGGATCa                                                                         >  4:796670/1‑150 (MQ=255)

                                                                        tcttcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAg                                                                      <  1:90061/150‑1 (MQ=255)

                                                                          ttcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGGATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGt                                                                    <  4:762595/150‑1 (MQ=255)

                                                                           tcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGa                                                                                                        >  3:52771/1‑113 (MQ=255)

                                                                           tcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGa                                                                                                        <  4:52771/113‑1 (MQ=255)

                                                                             cGCTCAATGATATTATCAAGCTGATCAAGCTCGTGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTg                                                                 >  2:260852/1‑150 (MQ=255)

                                                                              gCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAg                                                                                                                         >  1:860668/1‑93 (MQ=255)

                                                                              gCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAg                                                                                                                         <  2:860668/93‑1 (MQ=255)

                                                                               cTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGcac                                                                                                                      <  4:572456/95‑1 (MQ=255)

                                                                                  aaTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACCACCGACAGACTGAATGCGCCGTATAGTTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                            >  1:670816/1‑150 (MQ=255)

                                                                                  aaTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                            >  2:644173/1‑150 (MQ=255)

                                                                                  aaTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                            >  1:1131891/1‑150 (MQ=255)

                                                                                    tGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGc                                                          >  2:327575/1‑150 (MQ=255)

                                                                                    tGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGc                                                          >  2:680383/1‑150 (MQ=255)

                                                                                    tGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGc                                                          >  1:562493/1‑150 (MQ=255)

                                                                                       tattatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGAtgctg                                                                                                                                            >  2:933339/1‑65 (MQ=255)

                                                                                        attatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTa                                                      >  4:52562/1‑150 (MQ=255)

                                                                                         ttatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                            >  4:380648/1‑143 (MQ=255)

                                                                                         ttatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                            <  3:380648/143‑1 (MQ=255)

                                                                                          tatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAAt                                                    >  3:532560/1‑150 (MQ=255)

                                                                                           atCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAAtt                                                   <  2:1032319/150‑1 (MQ=255)

                                                                                            tCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTg                                                  >  2:935625/1‑150 (MQ=255)

                                                                                                gCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGatat                                              <  3:668437/150‑1 (MQ=255)

                                                                                                  tGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATAttt                                            <  2:653879/150‑1 (MQ=255)

                                                                                                  tGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATAttt                                            <  1:1675522/150‑1 (MQ=255)

                                                                                                    aTCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCa                                                                         <  2:760796/119‑1 (MQ=255)

                                                                                                    aTCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGt                                          >  4:172735/1‑150 (MQ=255)

                                                                                                      cAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCa                                        >  4:924244/1‑150 (MQ=255)

                                                                                                         gCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAAtt                                     >  3:452966/1‑150 (MQ=255)

                                                                                                           tCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGGTAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAAtttt                                   <  1:1305092/150‑1 (MQ=255)

                                                                                                           tCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAAtttt                                   >  2:1341589/1‑150 (MQ=255)

                                                                                                            cATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTa                                  <  4:909621/150‑1 (MQ=255)

                                                                                                            cATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTa                                  <  4:399537/150‑1 (MQ=255)

                                                                                                             aTGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCt                                                                                                                                     <  2:750892/50‑1 (MQ=255)

                                                                                                             aTGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCt                                                                                                                                     >  1:750892/1‑50 (MQ=255)

                                                                                                             aTGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTaa                                 >  1:1671236/1‑150 (MQ=255)

                                                                                                               gTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGcc                                                                                                 >  1:487294/1‑84 (MQ=255)

                                                                                                               gTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGcc                                                                                                 <  2:487294/84‑1 (MQ=255)

                                                                                                               gTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTattt                               >  2:500251/1‑147 (MQ=255)

                                                                                                                ttCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACAt                              >  3:243631/1‑150 (MQ=255)

                                                                                                                ttCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACAt                              >  1:385730/1‑150 (MQ=255)

                                                                                                                ttCGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACAt                              >  1:1540900/1‑150 (MQ=255)

                                                                                                                  cGTTGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCt                            <  2:562493/150‑1 (MQ=255)

                                                                                                                    ttGAGTGTGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCttt                          >  3:799309/1‑150 (MQ=255)

                                                                                                                        gtgtGAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAg                      <  4:622306/150‑1 (MQ=255)

                                                                                                                         tgtgAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCttttt                        <  2:911471/147‑1 (MQ=255)

                                                                                                                         tgtgAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCttttt                        >  1:911471/1‑147 (MQ=255)

                                                                                                                         tgtgAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAgg                     <  3:759163/150‑1 (MQ=255)

                                                                                                                         tgtgAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAgg                     <  1:935625/150‑1 (MQ=255)

                                                                                                                           tgAACGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGa                   >  1:777857/1‑150 (MQ=255)

                                                                                                                              aCGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCa                <  2:1540900/150‑1 (MQ=255)

                                                                                                                              aCGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCa                <  3:768494/150‑1 (MQ=255)

                                                                                                                               cGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaa               >  3:251110/1‑150 (MQ=255)

                                                                                                                               cGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaa               >  2:1202106/1‑150 (MQ=255)

                                                                                                                               cGGGATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaa               <  1:1341589/150‑1 (MQ=255)

                                                                                                                                 ggATGCAAAGCTGGCGGATGCTGCAATCCTGGCAATg                                                                                                                              <  1:730862/37‑1 (MQ=255)

                                                                                                                                    tGCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaagtaag          >  4:820359/1‑150 (MQ=255)

                                                                                                                                     gCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATa                                                                       >  3:102366/1‑88 (MQ=255)

                                                                                                                                     gCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATa                                                                       <  4:102478/88‑1 (MQ=255)

                                                                                                                                     gCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATa                                                                       <  4:102366/88‑1 (MQ=255)

                                                                                                                                     gCAAAGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATa                                                                       >  3:102478/1‑88 (MQ=255)

                                                                                                                                       aaaGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCt       >  2:1349125/1‑150 (MQ=255)

                                                                                                                                        aaGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTa      <  3:272365/150‑1 (MQ=255)

                                                                                                                                         aGCTGGCGGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTaa     >  3:802119/1‑150 (MQ=255)

                                                                                                                                            tggcggATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCa  <  2:469345/150‑1 (MQ=255)

CGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCA  >  NC_000913_3_KO_pgi/1399307‑1399596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`