Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	3,486,594	A→C	T159P (ACT→CCT)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	3,486,594	0	A	C	100.0%	322.5 / NA	90	T159P (ACT→CCT)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (48/42);  total (48/42)

ACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGT  >  NC_000913_3_KO_pgi/3486448‑3486736                                                                                                                                                   |                                                                                                                                               aCCCGGACATTCTGATGCGTTTGTCTGCACAGTTGGCGCGTCGTCTGCAGGACACTTCAGAGCACGTGGTCAACCTGGCGTTCCTCGACGTGACGTGCCGCATTGCTCAGACACTTCTGAATGTTGCAAAACACCCAGACAGTATGCCTc                                                                                                                                             >  2:725234/1‑150 (MQ=255)    cGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCAcc                                                                                                                                          <  4:94511/150‑1 (MQ=255)    cGGACATTCTGATGCGTTTGCCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCAcc                                                                                                                                          >  2:345401/1‑150 (MQ=255)          ttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACg                                                                                                                                    >  2:87173/1‑150 (MQ=255)            cTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGt                                                                                                                                  <  2:612423/150‑1 (MQ=255)                tGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGc                                                                                                                              <  4:253267/150‑1 (MQ=255)                  cGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCaa                                                                                                                            <  3:236104/150‑1 (MQ=255)                   gTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCaaa                                                                                                                           >  1:572716/1‑150 (MQ=255)                      tatCTACACAGATCGCGCGTCGTCTGCAAGTCACTTCACAGAAAGTGGGCAACCTGGCGTTCCTCTACGTGACCGGCCGCATTACACAGACTCTGCTGAATCTGCCAAAACAACCATACGCTATGCCTCACCCGGACGGTATGCAAATCa                                                                                                                        <  2:13779/148‑1 (MQ=255)                          tGCGCAGATGGCGCGTCGTCTGCAAGTCACGTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGCCGGTATGCAAATCAAAAt                                                                                                                    >  2:162698/1‑150 (MQ=255)                          tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAAt                                                                                                                    >  1:39336/1‑150 (MQ=255)                           gCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGt                                                                                                                                  >  1:791216/1‑135 (MQ=255)                           gCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGt                                                                                                                                  <  2:791216/135‑1 (MQ=255)                            cacaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCAATGCCTCACCCGGACGGTATGCAAATCAAAATTa                                                                                                                  >  1:609249/1‑150 (MQ=255)                             acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTAc                                                                                                                 >  4:48984/1‑150 (MQ=255)                                    gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAg                                                                                                          >  1:688455/1‑150 (MQ=255)                                      gcgTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGGATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGa                                                                                                        >  4:280090/1‑150 (MQ=255)                                      gcgTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAggg                                                                                                        >  3:321018/1‑149 (MQ=255)                                       cgtcgtCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGaa                                                                                                       >  4:389314/1‑150 (MQ=255)                                          cgtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAAtt                                                                                                    <  1:384164/150‑1 (MQ=255)                                           gtcTGCAAGTCACTTCAGAGAAAGTGGGCCACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                                                                                                   <  2:352290/150‑1 (MQ=255)                                            tcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTgg                                                                                                  >  1:48628/1‑150 (MQ=255)                                            tcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTgg                                                                                                  >  2:576667/1‑150 (MQ=255)                                             cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                                                 <  2:541097/150‑1 (MQ=255)                                             cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACCCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGa                                                                                                                                      <  1:685803/113‑1 (MQ=255)                                             cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACCCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGa                                                                                                                                      >  2:685803/1‑113 (MQ=255)                                               gCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCa                                                                                               >  2:764542/1‑150 (MQ=255)                                                  aGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGAt                                                                                            >  1:457650/1‑150 (MQ=255)                                                     cACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                                                         <  1:629850/150‑1 (MQ=255)                                                        ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                      <  4:187907/150‑1 (MQ=255)                                                                aaGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                              <  1:671408/150‑1 (MQ=255)                                                                aaGTGACCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCGCAGACTCTGCTGAATCTGCCAAAACAACCACACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTcctc                                                                              <  4:124107/150‑1 (MQ=255)                                                                 aGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                              <  2:522375/149‑1 (MQ=255)                                                                 aGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                              >  1:522375/1‑149 (MQ=255)                                                                  gTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt                                                                            >  4:353412/1‑150 (MQ=255)                                                                   tGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                                           <  1:332091/118‑1 (MQ=255)                                                                   tGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                                           >  2:332091/1‑118 (MQ=255)                                                                     ggCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaa                                                                         <  1:87374/150‑1 (MQ=255)                                                                      gCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaaa                                                                        >  1:641301/1‑150 (MQ=255)                                                                          ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGt                                                                    <  3:13330/150‑1 (MQ=255)                                                                           cTGGCGTTCCTCGACGCGACGCGCCGCATTGCACAGACTCTGCTGAATCTGGCAAATCAACCAGACGCTATGCCTCACCTGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGTTTTTCTCGTGAAACCGTg                                                                   <  4:243304/150‑1 (MQ=255)                                                                            tGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                  >  1:505779/1‑150 (MQ=255)                                                                               cGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCAGCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAc                                                               <  1:40835/150‑1 (MQ=255)                                                                               cGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAc                                                               <  1:293867/150‑1 (MQ=255)                                                                                    cgcGACGTGACCGGCCGCATTGCGCAGACCCTGCTGAATCTGGCACAACAACCAGACGCTAAGCCTCACCCGGGCGGGATGCAAATCAAACTTACCCGTCAGGAAACTGGACAGATTGTCGGCTGTTCTCGGGAAACCGTGGGAcccctt                                                          >  2:578508/3‑145 (MQ=255)                                                                                     tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCAc                                                                                                                                           <  4:60133/68‑1 (MQ=255)                                                                                     tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCAc                                                                                                                                           >  3:60133/1‑68 (MQ=255)                                                                                      cGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                                        >  1:538553/1‑150 (MQ=255)                                                                                        aCGTGACGGGCCGCATTGCACCNCCACTGCTGAATCCGGCAAAACAACCAGCCGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGCAACCGTGGGACGCATTCTGa                                                      <  4:107159/150‑1 (MQ=255)                                                                                        aCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGa                                                      <  1:87173/150‑1 (MQ=255)                                                                                          gTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAg                                                    <  4:515525/150‑1 (MQ=255)                                                                                               gggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt                                               >  4:522602/1‑150 (MQ=255)                                                                                                ggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                                              >  2:156394/1‑150 (MQ=255)                                                                                                 gCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTgg                                             <  2:538553/150‑1 (MQ=255)                                                                                                  ccGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                                            >  3:166058/1‑150 (MQ=255)                                                                                                    gCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCTGTAGGTATGCGTCGCCCGGAGAGTATGAAAACCGCAATTACCCGCCAGGCCATCGCGAAGATCGTCGGCTGTGCACGGGAAAGAGAGGGACGCATGCTGAAGACGATGGAAg                                          <  2:612036/150‑1 (MQ=255)                                                                                                     cATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGa                                         >  1:76151/1‑150 (MQ=255)                                                                                                     cATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGa                                         >  3:420546/1‑150 (MQ=255)                                                                                                       ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                       >  1:719068/1‑150 (MQ=255)                                                                                                       ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                       <  1:576667/150‑1 (MQ=255)                                                                                                       ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTAACAAGGTCAGCCTGAAAATCAAAATTACCCGTCAGGAAATTGTTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                       <  3:48984/150‑1 (MQ=255)                                                                                                        tGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGt                                                                                                             <  2:14781/79‑1 (MQ=255)                                                                                                        tGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGt                                                                                                             >  1:14781/1‑79 (MQ=255)                                                                                                            caGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAc                                  <  1:395936/150‑1 (MQ=255)                                                                                                                   tgctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCt                                <  4:332837/145‑1 (MQ=255)                                                                                                                   tgctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCt                                >  3:332837/1‑145 (MQ=255)                                                                                                                   tgctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtct                           >  2:205027/1‑150 (MQ=255)                                                                                                                     ctgAATCTGGCAAAATAACCGGACTCGGTGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGGCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAGTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc                         <  2:542154/150‑1 (MQ=255)                                                                                                                     ctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc                         >  3:469273/1‑150 (MQ=255)                                                                                                                     ctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc                         <  1:156394/150‑1 (MQ=255)                                                                                                                     ctgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTcc                         >  3:36456/1‑150 (MQ=255)                                                                                                                      tgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCaaaa                                                                                                                     <  1:294171/57‑1 (MQ=255)                                                                                                                      tgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCaaaa                                                                                                                     >  2:294171/1‑57 (MQ=255)                                                                                                                      cgAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCg                        >  3:132919/2‑150 (MQ=255)                                                                                                                       gAATCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGc                       >  1:699826/1‑150 (MQ=255)                                                                                                                        aaTCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                  >  2:342604/1‑106 (MQ=255)                                                                                                                        aaTCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                  <  1:342604/106‑1 (MQ=255)                                                                                                                        aaTCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGca                      <  3:353412/150‑1 (MQ=255)                                                                                                                          tCTGGCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcaca                    >  3:158073/1‑150 (MQ=255)                                                                                                                             ggCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                       <  2:348228/128‑1 (MQ=255)                                                                                                                             ggCAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                       >  1:348228/1‑128 (MQ=255)                                                                                                                               cAAAACAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTa               >  1:645560/1‑150 (MQ=255)                                                                                                                                 aaaCAACCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaaa             <  1:764542/150‑1 (MQ=255)                                                                                                                                   acaacCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAc           >  4:96469/1‑150 (MQ=255)                                                                                                                                    caacCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAcc          >  2:275570/1‑150 (MQ=255)                                                                                                                                     aacCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCa         >  2:217048/1‑150 (MQ=255)                                                                                                                                      acCAGACGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGCTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAt        <  2:572716/150‑1 (MQ=255)                                                                                                                                            cGCTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATcgtcgt  <  1:217048/150‑1 (MQ=255)                                                                                                                                              cTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAcc          <  1:328014/140‑1 (MQ=255)                                                                                                                                              cTATGCCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAcc          >  2:328014/1‑140 (MQ=255)                                                                                                                                                   |                                                                                                                                               ACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGT  >  NC_000913_3_KO_pgi/3486448‑3486736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑