Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	2,513,351	C→A	L104M (CTG→ATG)	nupC →	nucleoside (except guanosine) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	2,513,351	0	C	A	100.0%	188.0 / NA	53	L104M (CTG→ATG)	nupC	nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/30);  total (23/30)

TTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTTA  >  NC_000913_3_KO_pgi/2513207‑2513495                                                                                                                                                 |                                                                                                                                                 ttCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATc                                                                                                                                             <  2:532113/150‑1 (MQ=255) ttCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATc                                                                                                                                             <  1:826624/150‑1 (MQ=255)    gTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGt                                                                                                                                          >  2:366969/1‑150 (MQ=255)            cTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCa                                                                                                                                  <  2:159206/150‑1 (MQ=255)                   gAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACAtt                                                                                                                           >  3:413371/1‑150 (MQ=255)                        gTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGACTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgt                                                                                                                      >  2:778145/1‑150 (MQ=255)                            gAAAAACTGCTCGGATTTGCCAACGAAGGGACTACCTTCGTCTTTGGTAGCATGACTGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGACCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTg                                                                                                                  >  2:400737/1‑150 (MQ=255)                            gAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGc                                                                                                                                <  2:49856/136‑1 (MQ=255)                            gAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGc                                                                                                                                >  1:49856/1‑136 (MQ=255)                                   tGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                                                                           <  1:144565/150‑1 (MQ=255)                                            ttGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgcg                                                                                                  <  1:612458/150‑1 (MQ=255)                                               ccAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCaa                                                                                               <  2:163324/150‑1 (MQ=255)                                                cAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAt                                                                                              >  4:980/1‑150 (MQ=255)                                                 aaCGAAGGGACTAACTTCGTCTTTGGTAGCAGGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAtt                                                                                             <  2:725085/150‑1 (MQ=255)                                                    gAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGt                                                                                          <  2:346952/150‑1 (MQ=255)                                                         gACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCt                                                                                     <  3:95833/150‑1 (MQ=255)                                                               cTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCtctc                                                                               <  4:235083/150‑1 (MQ=255)                                                               cTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCtctc                                                                               >  3:424945/1‑150 (MQ=255)                                                                ttCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTcc                                                                              >  3:158219/1‑150 (MQ=255)                                                                  cGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATTGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaa                                                                            <  1:164393/150‑1 (MQ=255)                                                                  cGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTCGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaa                                                                            <  1:154975/150‑1 (MQ=255)                                                                   gTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTAGCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                           <  2:366098/150‑1 (MQ=255)                                                                    tCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAg                                                                          <  1:785833/150‑1 (MQ=255)                                                                    tCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAg                                                                          >  2:731083/1‑150 (MQ=255)                                                                        tGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCaa                                                                      <  1:366969/150‑1 (MQ=255)                                                                                 gaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAAc                                                                     >  1:473048/1‑142 (MQ=255)                                                                                 gaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAAc                                                                     <  2:473048/142‑1 (MQ=255)                                                                                  aatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGc                                                            >  1:100025/1‑150 (MQ=255)                                                                                       tCAAGGCCTGGCATTCTTCTTCCTGAACGTGCTGTGCCCAACCGTCTCTATCTCTGCAATGACCGGTATTCCCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACt                                                       >  2:338154/1‑150 (MQ=255)                                                                                       tCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACt                                                       >  4:188317/1‑150 (MQ=255)                                                                                       tCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACt                                                       >  2:512480/1‑150 (MQ=255)                                                                                        cgattccTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTGTGCAATGATCGGTATTCTCCAACACATTCTCGTGTTGCCTGTGATCATCCTCGCAATTAGTTTCCTGCTCTCCAAAGTCAACGGCATGAGGAAACTg                                                      <  2:457322/145‑1 (MQ=255)                                                                                        cAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTg                                                      <  3:394908/150‑1 (MQ=255)                                                                                        cAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTg                                                      <  2:336650/150‑1 (MQ=255)                                                                                        cAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATGCGCTTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTg                                                      <  2:36978/150‑1 (MQ=255)                                                                                            gCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAAt                                                  >  4:180603/1‑150 (MQ=255)                                                                                                     tcttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                                                                           >  2:359147/1‑84 (MQ=255)                                                                                                     tcttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                                                                           <  1:359147/84‑1 (MQ=255)                                                                                                          ttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGa                                                    <  1:273896/134‑1 (MQ=255)                                                                                                          ttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGa                                                    >  2:273896/1‑134 (MQ=255)                                                                                                                aaaGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTg                                                                                                            <  2:45981/72‑1 (MQ=255)                                                                                                                aaaGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTg                                                                                                            >  1:45981/1‑72 (MQ=255)                                                                                                                        gtgCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCt                      <  1:84196/150‑1 (MQ=255)                                                                                                                             cAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTc                 >  3:110571/1‑150 (MQ=255)                                                                                                                                tCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGt              <  2:100025/150‑1 (MQ=255)                                                                                                                                   tCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAt                                                                                              <  4:376128/67‑1 (MQ=255)                                                                                                                                   tCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAt                                                                                              >  3:376128/1‑67 (MQ=255)                                                                                                                                      ttATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGaaa        >  1:552330/1‑150 (MQ=255)                                                                                                                                         tctcTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACt     >  1:696213/1‑150 (MQ=255)                                                                                                                                           tctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACttt   <  1:351890/150‑1 (MQ=255)                                                                                                                                            ctGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTTa  <  1:400737/150‑1 (MQ=255)                                                                                                                                             tGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGa                          <  1:618812/125‑1 (MQ=255)                                                                                                                                             tGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGa                          >  2:618812/1‑125 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACTTTA  >  NC_000913_3_KO_pgi/2513207‑2513495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑