New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	3155217 =	75 (1.000)	12 (0.170)	12/250	7.1	14.4%	coding (2/957 nt)	yqhC	transcriptional activator of yqhD
	?	NC_000913_3_KO_pgi	3155241 =	73 (1.050)					intergenic (‑23/‑114)	yqhC/yqhD	transcriptional activator of yqhD/aldehyde reductase, NADPH‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACCCCAACCCGCATT  >  NC_000913_3_KO_pgi/3155092‑3155390                                                                                                                                                      |                                                                                                                                                      aaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATa                                                                                                                                                       <  2:611930/150‑1 (MQ=255)   cTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAgg                                                                                                                                                     <  3:285133/150‑1 (MQ=255)     aTTTTCTTTATTTATCAGGTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCACTTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCa                                                                                                                                                   <  4:438973/150‑1 (MQ=255)           tttATTTTTCATTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAt                                                                                                                                             >  4:329451/1‑150 (MQ=255)           tttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAt                                                                                                                                             >  4:337195/1‑150 (MQ=255)            ttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAtt                                                                                                                                            <  2:400010/150‑1 (MQ=255)                ttttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCa                                                                                                                                        <  2:829598/150‑1 (MQ=255)                   tCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGaa                                                                                                                                     <  2:700711/150‑1 (MQ=255)                    cAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGaaa                                                                                                                                    <  3:185413/150‑1 (MQ=255)                       tttATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGc                                                                                                                                         >  2:630768/1‑142 (MQ=255)                       tttATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGc                                                                                                                                         <  1:630768/142‑1 (MQ=255)                           ttaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCTGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGc                                                                                                                                                    <  3:223058/127‑1 (MQ=255)                           ttaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCTGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGc                                                                                                                                                    >  4:223058/1‑127 (MQ=255)                             aaCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTATGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTg                                                                                                                           >  2:731342/1‑150 (MQ=255)                             aaCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTg                                                                                                                           >  1:515386/1‑150 (MQ=255)                                    tCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCa                                                                                                                    <  1:202948/150‑1 (MQ=255)                                     ccGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAg                                                                                                                   >  4:429562/1‑150 (MQ=255)                                      cGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAgg                                                                                                                  >  3:226546/1‑150 (MQ=255)                                      cGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAgg                                                                                                                  >  4:104099/1‑150 (MQ=255)                                                cGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGa                                                                                                        <  3:59332/150‑1 (MQ=255)                                                 ggCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGAt                                                                                                       <  3:104099/150‑1 (MQ=255)                                                  gCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATc                                                                                                      >  2:595302/1‑150 (MQ=255)                                                   cAAATCTCTTCACGGTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATca                                                                                                     <  2:169895/150‑1 (MQ=255)                                                    aaaTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATcac                                                                                                    >  3:28873/1‑150 (MQ=255)                                                    aaaTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATcac                                                                                                    >  2:279210/1‑150 (MQ=255)                                                      aTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                                           <  3:138047/109‑1 (MQ=255)                                                      aTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                                           >  4:138047/1‑109 (MQ=255)                                                      aTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACaa                                                                                                  >  3:281433/1‑150 (MQ=255)                                                          ctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaa                                                                                              >  2:462058/1‑150 (MQ=255)                                                          ctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaa                                                                                              <  2:715275/150‑1 (MQ=255)                                                           ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaa                                                                                             >  1:534627/1‑150 (MQ=255)                                                           ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaa                                                                                             >  4:225822/1‑150 (MQ=255)                                                            tCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaa                                                                                            <  1:632724/150‑1 (MQ=255)                                                             cACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaaa                                                                                           >  3:475578/1‑150 (MQ=255)                                                             cACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaaa                                                                                           <  1:355579/150‑1 (MQ=255)                                                               cGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACa                                                                                         >  1:589827/1‑150 (MQ=255)                                                                  ttCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGTCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCa                                                                                      <  3:404631/150‑1 (MQ=255)                                                                  ttCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCa                                                                                      <  3:285192/150‑1 (MQ=255)                                                                  ttCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCAATCACAATGAAAAACATCa                                                                                      >  2:825797/1‑150 (MQ=255)                                                                          gcgTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATc                                                                              >  2:493743/1‑150 (MQ=255)                                                                               aTTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTtct                                                                         <  3:138871/150‑1 (MQ=255)                                                                                  tCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTg                                                                      <  3:116469/150‑1 (MQ=255)                                                                                    ttAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGcc                                                                    <  2:136201/150‑1 (MQ=255)                                                                                    ttAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGcc                                                                    >  1:806921/1‑150 (MQ=255)                                                                                      aGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCt                                                                  <  1:275937/150‑1 (MQ=255)                                                                                        gAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCa                                                                >  4:134862/1‑150 (MQ=255)                                                                                             atGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTg                                                           <  1:577862/150‑1 (MQ=255)                                                                                                 ggCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaaa                                                                                           >  1:391286/1‑114 (MQ=255)                                                                                                 ggCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaaa                                                                                           <  2:391286/114‑1 (MQ=255)                                                                                                  gCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCa                                                      <  4:264778/150‑1 (MQ=255)                                                                                                    aaTTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGc                                                                                   >  2:755525/1‑119 (MQ=255)                                                                                                    aaTTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGc                                                                                   <  1:755525/119‑1 (MQ=255)                                                                                                       ttGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACAt                                                                                        <  3:322900/111‑1 (MQ=255)                                                                                                       ttGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACAt                                                                                        >  4:322900/1‑111 (MQ=255)                                                                                                        tGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAg                                                <  3:260138/150‑1 (MQ=255)                                                                                                         gATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGATCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAgg                                               <  1:731342/150‑1 (MQ=255)                                                                                                         gATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAgg                                               <  3:429562/150‑1 (MQ=255)                                                                                                               gcgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCaa                                         <  1:118974/150‑1 (MQ=255)                                                                                                                cgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAg                                        >  4:370002/1‑150 (MQ=255)                                                                                                                cgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAg                                        >  1:482996/1‑150 (MQ=255)                                                                                                                cgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAg                                        >  2:489944/1‑150 (MQ=255)                                                                                                                   aaTTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTaa                                     >  1:718018/1‑150 (MQ=255)                                                                                                                      tttGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGa                                  <  2:96771/150‑1 (MQ=255)                                                                                                                       ttGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaa                                 <  1:825797/150‑1 (MQ=255)                                                                                                                            gCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACt                            <  4:226546/150‑1 (MQ=255)                                                                                                                             taTTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTg                                                           >  1:479381/2‑118 (MQ=255)                                                                                                                             taTTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTg                                                           <  2:479381/117‑1 (MQ=255)                                                                                                                                ttCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGCGCACTGAGAGCCTGGCCGCTGGCACTCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTaa                        <  1:246620/150‑1 (MQ=255)                                                                                                                                      aGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGca                  <  3:225822/150‑1 (MQ=255)                                                                                                                                      aGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGca                  <  3:419826/150‑1 (MQ=255)                                                                                                                                      aGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGca                  >  3:232466/1‑150 (MQ=255)                                                                                                                                          ctctGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACAcc              <  3:370002/150‑1 (MQ=255)                                                                                                                                           tctGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCa                                                      <  2:699918/109‑1 (MQ=255)                                                                                                                                           tctGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCa                                                      >  1:699918/1‑109 (MQ=255)                                                                                                                                           tctGGAGAAATAGGCAAGACAGTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCACCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACAccc             <  2:589827/150‑1 (MQ=255)                                                                                                                                              ggAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAgg                                                                                    <  2:138540/76‑1 (MQ=255)                                                                                                                                              ggAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAgg                                                                                    >  1:138540/1‑76 (MQ=255)                                                                                                                                               gagaAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACCCCAAc         >  3:201840/1‑150 (MQ=255)                                                                                                                                               gagaAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCACTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACCCCAAc         >  1:100146/1‑150 (MQ=255)                                                                                                                                                  aaaTAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACCCCAACCCg      <  2:534627/150‑1 (MQ=255)                                                                                                                                                      aGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACCCCAACCCGCAtt  <  2:718018/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      AACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACCCCAACCCGCATT  >  NC_000913_3_KO_pgi/3155092‑3155390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.