| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,187,215 | C→G | D622E (GAC→GAG) | rpoC → | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,187,215 | 0 | C | G | 98.2% | 172.1 / NA | 56 | D622E (GAC→GAG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base G (22/32); minor base T (0/1); total (23/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGT > NC_000913_3_bme_pgi/4187067‑4187357 | cTGTGGGCGGTGCAATAGTGTGGATGATTGGGCCGAAAGGTCGGCCTTGCTCCATCGTCAGCCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCAGTCTCGGTCGGAAACCGACCGTTATTTTTGCGGAc < 2:622957/149‑1 (MQ=255) tGGCCGGGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGa < 2:310237/149‑1 (MQ=255) gCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTCCTCCGTCGTTGGTCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATc < 1:23231/149‑1 (MQ=255) tgtgGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCAAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGc < 2:443300/149‑1 (MQ=255) ggATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTc > 2:50486/1‑149 (MQ=255) gatgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGTCTTCg > 2:50404/1‑149 (MQ=255) gatgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCCCATTCTCGATCTGAAACCGACCGTTATTTTTGCGGCGCAGATCATGTACACCGGCTTcc > 2:87339/1‑148 (MQ=255) gatTGTAGCGAAAGGTCCGCATTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACGCCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCt < 2:749865/149‑1 (MQ=255) tACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCa < 1:477376/149‑1 (MQ=255) ccGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGGCAGATCATGTACACCGGCTTCGCCTATGCAgc > 1:53498/1‑149 (MQ=255) ccGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCAGGGTAAAAAAGCAATCTCCAAAATGCTGAAAACCTGCTACCGCATTCCCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCCTCGCCTATGccgg > 2:33667/1‑146 (MQ=255) gAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGACTTCGCCTATGCAgcgc > 2:667884/1‑149 (MQ=255) gAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTAAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAgcgc > 2:44908/1‑149 (MQ=255) gAAAGGGGTGCCTTACTCCAACGCTAAACAAGCGCGGGGTAAAAAAGCGATTTCCAAAAGGCTGAACACCTGCTACCGCATGCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAgcgc < 2:427148/149‑1 (MQ=255) ccTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGc > 1:717294/1‑149 (MQ=255) ccacacacAACCAGGCGCTGGGTAAAAAAGCAATCCCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt < 2:280716/142‑1 (MQ=255) cATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTAGACCGGCTTCGCCTATGCGGGCCGTTCTGGTTCATCTGTTg > 1:172971/1‑149 (MQ=255) cATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCGGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTg < 2:509605/149‑1 (MQ=255) cGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTa < 1:681373/149‑1 (MQ=255) cGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTa < 1:778605/149‑1 (MQ=255) acgacgcgcTGGGTAAAAAAGCAACCTCCAAACTGCTGAACAGCTGCTACCGCATTCTCTGTCTGAAACCGACCGTTATTTTAGCGAAGCTGGTGAGTTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGag < 2:490722/144‑2 (MQ=255) cAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGa < 2:547165/149‑1 (MQ=255) cAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGt < 1:313706/129‑1 (MQ=255) cAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGt > 2:313706/1‑129 (MQ=255) aGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGAc > 1:775865/1‑149 (MQ=255) gcgtggTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATgg < 1:87583/145‑1 (MQ=255) gCAATCTCCAAAATGCTGCACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGagaa < 2:306315/149‑1 (MQ=255) cAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGagaag < 1:696265/149‑1 (MQ=255) aTCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAgaagaa < 1:44908/149‑1 (MQ=255) tcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCAGGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAaca > 2:647526/1‑149 (MQ=255) tcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTAAAACCGACCGTTATACTTGCGGAGCAGAGCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGTTATCGAGGACATGGTCATCCCGGAGAAGAAaca > 2:291210/1‑149 (MQ=255) ccAAAATGCTGAACACCTGCTACCGCCTTCTCGGTCTGAAACCGACCGTTATTTTTGCGGATCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAacac < 1:107611/149‑1 (MQ=255) ccAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATTGTCGTCCCGGAGAAGAAacac < 2:452362/149‑1 (MQ=255) ccAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAacac < 2:796230/149‑1 (MQ=255) ccAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCGATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAacac < 1:352510/149‑1 (MQ=255) cTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatcat > 1:542011/1‑149 (MQ=255) ccTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAg < 1:729706/149‑1 (MQ=255) cttcTACCGCACTCTCGGTCTGACACCGATCGTTATTTTTGCGGCGCAGGCCACGTACACAGGCTTGGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAgg < 1:506861/146‑1 (MQ=255) cTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACAGGGTCATCCCGGAAAAGAAAACCCAAATCACCTcccggg > 2:375832/1‑145 (MQ=255) cTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcag > 2:152399/1‑149 (MQ=255) ccGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaag < 1:283278/149‑1 (MQ=255) cgaaTTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagc < 2:660783/146‑1 (MQ=255) cGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagc > 1:213606/1‑149 (MQ=255) ttCTCGGTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTAATCCCGGAGAAGTAACACGAATTCATCTCCGAGgcagaagcagaa < 1:635727/149‑1 (MQ=255) ggTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGc < 2:572601/149‑1 (MQ=255) gTCTGAAACCGAGCGGTATGTATGCGGAGCAGATCATGTAGCACGGCTTCGCCTATGCATCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAAGCATCTCCGAGGCAGAAGCAGAAGTTGCt < 2:453980/149‑1 (MQ=255) gTCTGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCTAGGCAGAAGCAGAAGTTGCt < 2:53498/149‑1 (MQ=255) gTCTGAAACCGACCGGTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCt < 1:132220/149‑1 (MQ=255) tGAAACCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaa > 1:358451/1‑149 (MQ=255) aaaCCGACCGTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAAt > 1:321359/1‑149 (MQ=255) cggccgGTATTGTTGGGGAGCAGGTCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAg < 2:290435/146‑1 (MQ=255) accgTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCAGCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCGTATCCGAGGCTGATGCGGAGGTTGCTGAAGttgcgga > 1:123523/1‑144 (MQ=255) gTTATTTTTGCGGAGCAGATCATGTACACTGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCTCGGAGAAGAAACACGAAATCATCTCTTATGCGGAAGCAGAAGTTGCTGAAATTCAGGAGCa < 1:390873/149‑1 (MQ=255) gTTATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGACCa > 2:374063/1‑149 (MQ=255) ttATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTAAGGAGCAg > 1:35305/1‑149 (MQ=255) ttATTTTTGCGGAGCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCGCCGAGGCAGAAGCAGCAGTTGCTGAAATTCAGGAGcgg > 1:215876/1‑147 (MQ=255) tGCGGAGCAGATCATGTTCTCCGCCTTCGCCTACGCAGCGCGTTCTGGTGCATCTGTTGGTATCGAGGACAAGGCCATCCCGGAGAAGAAACACGAAATGATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgt < 2:401853/149‑1 (MQ=255) | CTGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGT > NC_000913_3_bme_pgi/4187067‑4187357 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |