New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi 19797 =NA (NA)53 (1.180) 39/288 0.2 94.7% intergenic (+177/+14) nhaR/insB1 transcriptional activator of nhaA/IS1 transposase B
?NC_000913_3_bme_pgi 61481 = 3 (0.070)coding (1784/2907 nt) rapA RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase

GAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_bme_pgi/19944‑19797
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGTGTCTTCTTCGGCAAACCAGGCGGCA  >  NC_000913_3_bme_pgi/61481‑61627
                                                                                                                                                                                                                                                                                                       
GAATTGAGCGGCATAACCTGAATCTGAGGCAGCACGTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCGCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTGGGAGTCATTACC                                                                                                                                                    <  2:432156/149‑1
    TGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACTGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAAT                                                                                                                                                <  2:410578/149‑1
                CCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCGATACGCTGCTCCAG                                                                                                                                    >  1:335792/1‑149
                CCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTATCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCACTACGCTGCTCCAG                                                                                                                                    >  1:115908/1‑149
                 CTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGT                                                                                                                                   >  2:69104/1‑149
                  TGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTA                                                                                                                                  >  1:46517/1‑149
                         GAGGCAGTACGTGGGACGGGTGGTACGGAAGTCGTTGTCGTACTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAATATATCACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGG                                                                                                                           <  2:244739/149‑1
                            GCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTT                                                                                                                        >  2:134380/1‑149
                            GCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTT                                                                                                                        >  1:597516/1‑149
                               gaaaCTGGCACGGCGGGGACGGAAGTCGCTGTCGTTTTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAAGACGCTGCTCCAGTAGAGCCGGGTTGAA                                                                                                                     <  2:356579/145‑1
                                 ACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATG                                                                                                                   <  1:421113/149‑1
                                      gcagcGCTGGGACGGAAGTCGCTTGCGTGCTCAAAATCGGTGGAGCTGAATGACAAAGTCATCGGGCATTATCTGCACATAAAACCCTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGG                                                                                                              <  2:466862/144‑1
                                      GCACGGCTGGGACGGAAGTCGCTGTGGTTCTCAAAAGCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGG                                                                                                              <  2:150441/149‑1
                                      GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAACTCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAA                                                                                                                     >  1:320588/1‑142
                                      GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAA                                                                                                                     >  1:721469/1‑142
                                      GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAA                                                                                                                     <  2:721469/142‑1
                                      GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAA                                                                                                                     <  2:320588/142‑1
                                                   GGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCA                                                                                                 <  1:69104/149‑1
                                                           CTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGACAAACATCACCATGTGGCTG                                                                                         >  2:378995/1‑149
                                                                GTTCCCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTCGCTCCGGTGTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAA                                                                                    <  1:593399/149‑1
                                                                      AAAATCGTTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAA                                                                              <  1:413955/149‑1
                                                                      AAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAA                                                                              <  1:138328/149‑1
                                                                      AAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAA                                                                              <  2:55244/149‑1
                                                                      AAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAA                                                                              <  1:37912/149‑1
                                                                      AAAAGCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAA                                                                              <  1:659777/149‑1
                                                                       AAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAG                                                                             >  2:43189/1‑149
                                                                          gCGGTGGAGCTGGATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTA                                                                          <  1:392672/148‑1
                                                                           CGGGGGAGCTGCATGACAAAGCCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTAC                                                                         <  1:331083/149‑1
                                                                             GTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAAGAAGTTGGAGTCATTACCCAAGACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCACAGGTTATCAGGTGGCTGGCGAACTGGAAGTTACGT                                                                       <  1:191831/149‑1
                                                                                  gcgGCATGACAAAGTCATCGGGCATTATCTGAACATAACACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTC                                                                  <  2:75128/146‑1
                                                                                  GCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGT                                                                                                             <  1:679863/106‑1
                                                                                  GCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGT                                                                                                             >  2:679863/1‑106
                                                                                   CTGCATGACAAAGTCATCGGGCATTATCTGAACATAGAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCA                                                                 <  1:647589/149‑1
                                                                                      caaGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGCGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAA                                                              <  1:667922/146‑1
                                                                                       ataACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGAGGGAGTCATTCCCCAATACGCTGCTCCAGTAGATCCCGGGTGAATGGCAGGTCAAACATCCCCATGTGGCTGACGAACGGGACGTTGCGTCCTTCAGAAC                                                             >  2:211664/4‑149
                                                                                        TGACAATGTTATCGTGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCGCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACC                                                            <  2:745446/149‑1
                                                                                           CAAAGTCATCGGGCATTATCTGAACATAAAACACTGTCCAGAAGTTGGATTCATTAGCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGAT                                                         <  1:766835/149‑1
                                                                                           CAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGAT                                                         >  2:770250/1‑149
                                                                                           CAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATATGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGAT                                                         >  2:378625/1‑149
                                                                                                     GGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAACCATCACCAGGTGGCTGGCGAACTGTAAGTTACGTCCTGCAGAACCAATTTCTGATCAC                                               >  1:324223/1‑149
                                                                                                         ATTATCTGACCATAAGACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCA                                           >  2:495332/1‑149
                                                                                                            ATCTGAACATAAAACACTATCAATAAGTTGGATTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACACCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTGCTGAGCACAGCAGTA                                        <  2:38955/149‑1
                                                                                                             TCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTAC                                       <  1:375011/149‑1
                                                                                                                 AACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAA                                                                              >  2:347091/1‑106
                                                                                                                 AACATAAAACACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAA                                                                              <  1:347091/106‑1
                                                                                                                     TAAAACACTATCAATAAGGTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGC                               <  2:509218/149‑1
                                                                                                                         tCACTATCAATAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGT                           <  1:345634/148‑1
                                                                                                                              ATCAATAAGTTAGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGTGTCTT                      >  1:501727/1‑149
                                                                                                                                 aagtAGTTGGGGGCATTCCCCAATACGCTGCTCCAGTAGATCCGGGTAGAATGGCAGGTCAAGCATCACCATGTGGCTGGCGAACTGGAAGTTACGTCGTTCAGTAACGATTTCTGAGCACAGCAGTACCTGTGCGCCGGTGTCTTCTT                   <  2:412372/145‑1
                                                                                                                                  ATAATTTGGACGCATGACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTTGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGTGTCTTCTTC                  <  2:55340/149‑1
                                                                                                                                  ATAAGATGGCGTCATTACCCAATACTCTGCTCCAGTAGCTCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACTGCAGTACCTGTGCGCCGGTGTCTTCTTC                  <  2:451572/149‑1
                                                                                                                                   cAAGTTGGAGTCATTACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGTGTCTTCTTCG                 <  1:134187/148‑1
                                                                                                                                              cagTACCCAATACGCTGCTCCAGTAGATCCGGGTTGCATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGTGTCTTCTTCGGCAAACCAGGC      <  2:56032/146‑1
                                                                                                                                                  ACCCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGTGTCTTCTTCGGCAAACCAGGCGGCA  <  2:597516/149‑1
                                                                                                                                                                                                                                                                                                       
GAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_bme_pgi/19944‑19797
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCAATACGCTGCTCCAGTAGATCCGGGTTGAATGGCAGGTCAAACATCACCATGTGGCTGGCGAACTGGAAGTTACGTCCTTCAGAACCGATTTCTGAGCACAGCAGTACCTGTGCGCCGGTGTCTTCTTCGGCAAACCAGGCGGCA  >  NC_000913_3_bme_pgi/61481‑61627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.