| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 4,233,746 | A→G | intergenic (‑513/‑2161) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 4,233,746 | 0 | A | G | 98.3% | 170.5 / NA | 59 | intergenic (‑513/‑2161) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (37/21); minor base T (0/1); total (37/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCT > NC_000913_3_bme_pgi/4233599‑4233891 | tCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCGAAGTCACAATTCTCAAAATCagagga > 1:667342/1‑150 (MQ=255) tCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCTCAATTCTCAAAATCAGAGGAGTAtt > 2:235822/1‑150 (MQ=255) cTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCACAGTCACAATTCTCAAAATCAGAGGAGTATTGc < 1:621774/150‑1 (MQ=255) ggTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATg > 1:559033/1‑150 (MQ=255) tCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGAATGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGc > 1:677539/1‑150 (MQ=255) cAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCa > 2:368467/1‑150 (MQ=255) ttagtTCAGCTAATAAATTCTTCACAGAGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAag < 1:428270/149‑1 (MQ=255) gtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAag < 1:11980/150‑1 (MQ=255) taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAg > 1:922390/1‑150 (MQ=255) aaaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAATATCAGAGGAGTATTGCTAATGGCAAGAGACGcgacgacga > 1:52702/1‑147 (MQ=255) aaaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGc < 2:24939/150‑1 (MQ=255) aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCt > 1:390149/1‑150 (MQ=255) gCTTCACTGCGCTAAGGGATTACACTCAACAAAACGCTAACCGCACTAAAACCATCACAATTTTCTGGGACTGGCGCTACAATCATCCAAAGTCACAATTCTCAAAACCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTGGa < 2:723626/150‑1 (MQ=255) tCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGAGCTAAAATCTTCCAAAGTCACAATTCTAAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGc > 1:509641/1‑150 (MQ=255) cTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAAc < 2:339309/150‑1 (MQ=255) cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGt > 1:746867/1‑150 (MQ=255) atatgggATTACACTCAAGAATACGCTAACGGCACTACAACCATCACATTTTTCTGTGACTGTCCCTACAATCTTCCAATGTCACAATTCTCAAAATCAGAGGAGTTTTGCTAATGGCTAGAGACGCGACGAAGCTTGAAGCAACGGTCg < 2:256947/146‑1 (MQ=255) tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGc < 2:559033/150‑1 (MQ=255) ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAg > 1:87036/1‑150 (MQ=255) cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaa > 1:894260/1‑150 (MQ=255) cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACTGTCGCAAAGCTGaaaaa > 2:923597/1‑150 (MQ=255) cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa > 2:471409/1‑150 (MQ=255) cAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCGAAACTCACAATTGTCAAAATCAGAGGAGTATTCCTAATGGCAAGAGACGCGACGAAGCTGGAACCAACGGTCGCAAAGCTGCAAAAGc > 1:318779/1‑150 (MQ=255) cAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGc > 1:673003/1‑150 (MQ=255) cAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGc > 1:5937/1‑150 (MQ=255) cAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATATTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGAAAGAGACGCTACGAAGCTTGAAGCAACGGTCTCAAAGCTGAAAAAGc > 1:479861/1‑150 (MQ=255) tACGCTATAGGCACTAAAACCATCTCATTTTTCTGTGACTGGCGCTATTATCTTCCAAAGTCACAATTCTCTAAATCAGAGGAGTTTTGCTAATTGCATGAGACGCGACGAAGCTTGATGCAACGGTCGCAAAGCTGAAAAAGCTTTggg < 2:712883/150‑1 (MQ=255) aaCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAAt > 2:865946/1‑150 (MQ=255) cGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTATTGCAAATAGACGCGACGAAGCTTGAAGCAACGCTCGAAAAGCTGATAAAGAATTGTGCgaatacg > 1:607015/1‑144 (MQ=255) gCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGCGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGc < 1:87604/150‑1 (MQ=255) cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc > 1:417170/1‑150 (MQ=255) aaaCCATCACATATTTCTGTGAGTGGCGCTACAATCTTCCAAAGTCAAAATTCTCAAACTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACCGTCGCAATGCTGAAAAAGCTTTGGGCGGATTCGTCACcgcg > 1:158596/1‑150 (MQ=255) aCCATCACATTTTTCTGTGACTGGCGCAACAATCTTCCAACGGCACAATTATCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGGTGAAAAAGCATTGGGCGGTATCGGCCCcgcgcg < 2:52702/150‑1 (MQ=255) ccATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa < 1:142398/150‑1 (MQ=255) aTCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGata > 1:701040/1‑150 (MQ=255) aacaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGCCGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATg < 2:722113/149‑1 (MQ=255) aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc > 1:760055/1‑150 (MQ=255) aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc > 1:764182/1‑150 (MQ=255) gtgACTGGCGCTACAATCTTCCAAAGTCACACTTCTCAAAATCCGAGGAGTTTTGCTAATGGCAAGAGACGAGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCTGCAtt < 2:509641/150‑1 (MQ=255) gtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTGATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCTTTGGGCGGAATCGGCCCCGCGCGCTATGCGCGCGGGAtt > 2:711398/1‑150 (MQ=255) gtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTCAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAtt > 2:136352/1‑150 (MQ=255) aaTCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccg < 2:417170/150‑1 (MQ=255) catCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGCCGCGACGAAGCTTGAAGCAACGGTCGCAAAGCGGAACAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggcc < 1:896043/148‑1 (MQ=255) cTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggcc > 2:909531/1‑150 (MQ=255) gTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg > 1:536430/1‑150 (MQ=255) gTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCCGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg > 1:579970/1‑150 (MQ=255) tCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGc < 2:746867/150‑1 (MQ=255) cacaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGACGGAATCTGCCAAGCGCAATAAGCGAGCCGCATACAGCGCCGCCCCCGGACGGTGCGGGcc > 2:829366/1‑149 (MQ=255) caATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGat > 1:318534/1‑150 (MQ=255) aaaTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGat < 1:368467/149‑1 (MQ=255) aaTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGata > 2:342929/1‑150 (MQ=255) aTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAGGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGCCCCCGGCCGGTTCGGGCGatat > 2:381494/1‑150 (MQ=255) tctcAAAATCAGAGGAGTATTGCTAAGGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTc < 2:361818/150‑1 (MQ=255) tcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGc < 2:390149/150‑1 (MQ=255) aaaaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg > 2:364476/1‑138 (MQ=255) aaaaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg < 1:364476/138‑1 (MQ=255) aaaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGCCCCCGGCCGGTTCGGGCGATATTCGctct > 1:585014/1‑150 (MQ=255) tCAGATGAGTATTGCTATTGGCAAGAGACGCTTCGTAGCTTGAAGCAAGGGTCGCAAAGCTGAAAAATCATTGGGCGGATTCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGcc < 2:318779/150‑1 (MQ=255) tCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGcc > 1:281707/1‑150 (MQ=255) cAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGAGCCGCCCCCGGACGGTTCGGGCGATATTCGCTCTGccg > 2:438607/1‑149 (MQ=255) | TCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCT > NC_000913_3_bme_pgi/4233599‑4233891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |