Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,734,142	C→A	V141V (GTC→GTA)	xylH →	D‑xylose ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,734,142	0	C	A	100.0%	110.8 / NA	38	V141V (GTC→GTA)	xylH	D‑xylose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/13);  total (25/13)

CTGTTAGGTGGCGTCGCGGCGATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGC  >  NC_000913_3_bme_pgi/3733996‑3734286                                                                                                                                                   |                                                                                                                                                 cTGTTAGGTGGCGTCGTAGCGATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAAc                                                                                                                                                <  1:748289/149‑1 (MQ=255)              tCGCGGCGATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCCCTGGCATg                                                                                                                                   >  2:227572/1‑149 (MQ=255)                gcggcgATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGCCTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGtt                                                                                                                                 <  1:539372/149‑1 (MQ=255)                  ggcgATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTGCCGTCATTTATGGTAACCCTCGCGGGCATGTTgg                                                                                                                               >  1:354289/1‑149 (MQ=255)                           tgACGTCTGGTTTGGGTGGTCATTGCTATTTTGTATCATTGGGACGCTGGTTGCGGGACTGCCTCGCGGTGCCTGGGACGGATGGTGGGTCGCGTGCAGTAAAGGCCCTTCGTTTATTGTAACCCTCGCGGGCATGTTGGCATTTcgcg                                                                                                                      <  2:513535/149‑1 (MQ=255)                           tgACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTcgcg                                                                                                                      <  1:225456/149‑1 (MQ=255)                             cccgcgggTTGGACTGGCCTGTGCCAATTACCTTCATTGTGGCGCTGGTCCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGc                                                                                                                    <  2:595035/143‑1 (MQ=255)                               gTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCAt                                                                                                                  >  1:124886/1‑149 (MQ=255)                                    gTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCa                                                                                                             >  2:168009/1‑149 (MQ=255)                                         gCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTTCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCGTATATTGTAAACTTCTCGGGGAAGTTGCAATTTAGCGGAATACTCATTgcg                                                                                                        >  2:500171/1‑147 (MQ=255)                                         gCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGc                                                                                                        >  1:717188/1‑149 (MQ=255)                                          cTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACACTCACGGGCATGTTGGCATTTCGCGGCATACCCATTGGCa                                                                                                       >  2:164862/1‑149 (MQ=255)                                                  tGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATTCTCATTGGCATCACCAAc                                                                                               <  1:285020/149‑1 (MQ=255)                                                     cACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGc                                                                                            >  1:193877/1‑149 (MQ=255)                                                      aCTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAGAGTCCCTTCATGTGTTGTAACGGTCGCGGGCGTGTTGTCATTTCGCGGCACACACATTGGCCTCGCCGACGGCa                                                                                           >  2:327833/1‑149 (MQ=255)                                                            catcatTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTg                                                                                     >  1:24704/1‑149 (MQ=255)                                                             atcacTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGCTGGTGGGTCGCGTACCGTAAAGTCCCTTCATCCATTGTACCCCTCGGGGGCATGTTGGCATTTCGCGGCGTACTCATTGGCAGCACCCACGGCaacaccgt                                                                                    >  2:585920/1‑142 (MQ=255)                                                                      aCGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCccac                                                                           <  2:193877/149‑1 (MQ=255)                                                                      aCGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCccac                                                                           >  2:123577/1‑149 (MQ=255)                                                                                 gggACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGa                                                                >  1:131651/1‑149 (MQ=255)                                                                                     cTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTc                                                            >  2:20674/1‑149 (MQ=255)                                                                                     cTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCGTCATATATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCCCCGGGATGTc                                                            >  1:196106/1‑149 (MQ=255)                                                                                              ggTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTgg                                                   <  2:639835/149‑1 (MQ=255)                                                                                                        aCGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATGTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTAt                                         <  1:548736/149‑1 (MQ=255)                                                                                                         cGGATTGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAATCCTCGCGTGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTAtc                                        <  1:89/149‑1 (MQ=255)                                                                                                            aTGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTcc                                     >  2:559459/1‑149 (MQ=255)                                                                                                                      gcgTACCGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATACCCCACCAGCGCCGCGATGTCACAAATTGGGCAAACCTATCTCcccccccgccc                           >  2:110088/1‑144 (MQ=255)                                                                                                                            cGTAAAGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTCCCGGCtt                     >  2:332479/1‑149 (MQ=255)                                                                                                                                 aGTCCCTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtcatca                >  2:523972/1‑149 (MQ=255)                                                                                                                                      cTTCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGgc           >  1:117263/1‑149 (MQ=255)                                                                                                                                       ttCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGCGTTCATCATTGgct          >  1:332114/1‑148 (MQ=255)                                                                                                                                        tCATTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGCCTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAGGCTATCTCCCCGCCAGTACCGGCTTCGTCATTGgcgc         >  1:172918/1‑149 (MQ=255)                                                                                                                                          aTTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAACTGGGCAAAGCTAtctc                                      >  2:162269/1‑118 (MQ=255)                                                                                                                                          aTTTATTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAACTGGGCAAAGCTAtctc                                      <  1:162269/118‑1 (MQ=255)                                                                                                                                           tttATTGTAACCCTCGCGGGCATGTTGGCATTTCACGCCAGATTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCCCAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGACTTCATCATTGgcactct      >  2:165585/1‑144 (MQ=255)                                                                                                                                             tATTGTAACCCGCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTg    <  1:318728/149‑1 (MQ=255)                                                                                                                                              aTTGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTgg   <  1:594132/149‑1 (MQ=255)                                                                                                                                               ttGTAACCCTCGCGGGCATGTTGGCATTTCGCGGCATAATCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGc  >  1:303960/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CTGTTAGGTGGCGTCGCGGCGATTTGTGACGTCTGGTTAGGCTGGCCTTTGCCACTTACCATCATTGTGACGCTGGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGC  >  NC_000913_3_bme_pgi/3733996‑3734286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑