| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_bme_pgi | 1,195,468 | G→T | V116F (GTT→TTT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_bme_pgi | 1,195,468 | 0 | G | T | 96.3% | 79.3 / NA | 27 | V116F (GTT→TTT) | icd | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (14/12); minor base C (0/1); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATC > NC_000913_3_bme_pgi/1195332‑1195611 | cGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGcc > 1:2109/1‑149 (MQ=255) gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAg < 2:104370/149‑1 (MQ=255) aTCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTCGTGGCGTTCTCGCTCTGAACTTTGCCCTGCGCCAGGAACTgg < 1:570999/149‑1 (MQ=255) aGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTAc < 1:713151/149‑1 (MQ=255) aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCg < 2:42339/149‑1 (MQ=255) tGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTGAAGTTCCGCTGATCACTCCGGTTGGTGGCGGTATTCGCTCCCTGAAGTTTGACCTGCGTCAGAAACTGGATCTCTACAGCTGCCTGCGTCGGATACg > 2:251770/1‑149 (MQ=255) tGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACAACTCCGGTTGGTGGCGGTATTCGCTCTCTGAAATTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCGTGCGTCGGGTGCg > 1:518793/1‑149 (MQ=255) ggaggcctgtTCAAACTCGTGATGTGATTGGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGTTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGt < 2:74776/145‑1 (MQ=255) ggCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCCCTGAACTTTGCCCTGCGCCAGGAACAGGATCTCTACACCCGCCTTCGACCGCTACGt > 2:726890/1‑149 (MQ=255) gctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTCCTCTCa > 1:524373/1‑149 (MQ=255) aTCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGc > 2:463071/1‑149 (MQ=255) ttCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGtt > 1:519490/1‑149 (MQ=255) tCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTa > 2:288099/1‑149 (MQ=255) gAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGGATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCGAAGCCCGGTTAAaca > 1:195223/1‑149 (MQ=255) cgTTGCGATTAAAGGTCCGCTGGCCACTCCGGTTGGTGGCGGTAGTCGCCCTCTGAACCTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAAc < 1:345492/149‑1 (MQ=255) tGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGa < 2:298941/149‑1 (MQ=255) gCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGAc > 1:419168/1‑149 (MQ=255) gCCATTAAAGGACCGCTGACCACTCCGGTTGGTGGCGGGATTCGCCCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGTCTGCGTCCGGTACGTTACTTCGAGCGCGGACTTGGCCCGGTTAAACACCCTGAACTGAc < 1:378461/149‑1 (MQ=255) ggTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGt < 1:463071/149‑1 (MQ=255) tGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCTAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCAGTCCAAGCCCGGTTAAACACCCTGAACTGGCCGATATGGTTATCTTc > 2:213755/1‑149 (MQ=255) cGGTTGGTGGCGGTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAAc > 2:337098/1‑149 (MQ=255) gtggTATTCGATCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGAc < 1:464508/147‑1 (MQ=255) ggTGTTCGCTCTCTGAACTTTGCCATGCGCCAGCAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACAt < 2:320766/149‑1 (MQ=255) ggTATTCGCTCTCTGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACAt > 1:739481/1‑149 (MQ=255) atctctGAACTTTGCCCTGCGCCAGAAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAAATGCCCGATATGGTTACCTTCCGTGAAAACTCGGAAGACATTTATGCgg < 1:732343/148‑1 (MQ=255) tGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATc < 1:337098/149‑1 (MQ=255) tGAACTTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATc > 1:667344/1‑149 (MQ=255) | CGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATC > NC_000913_3_bme_pgi/1195332‑1195611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |