New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 1207805	4 (0.070)	46 (0.860)	36/266	0.4	91.5%	coding (305/630 nt)	stfP	e14 prophage; uncharacterized protein
	?	NC_000913_3_bme_pgi	= 1209602	5 (0.090)					pseudogene (18/501 nt)	stfE	pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related

CACGTAAATCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGA  >  NC_000913_3_bme_pgi/1209454‑1209751                                                                                                                                                     |                                                                                                                                                      cACGTAAATCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGc                                                                                                                                                      <  1:805044‑M2/150‑2 (MQ=255) cACGTAAATCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGc                                                                                                                                                      <  1:685105‑M2/150‑2 (MQ=255)  aCGTAAATCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGcc                                                                                                                                                     <  1:761986‑M2/150‑3 (MQ=255)  aCGTAAATCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCAGCACCATTGCATTTCAGCCAGCCTGATGGCGGAGTGGCTGAAGGCCACGGAGCCGGGACCCCAACCGGTAATGCGGAGcc                                                                                                                                                     <  1:662545‑M2/150‑3 (MQ=255)       aaTCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCTGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctc                                                                                                                                                >  2:691619‑M2/1‑143 (MQ=255)       aaTCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctc                                                                                                                                                >  2:460553‑M2/1‑143 (MQ=255)       aaTCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTGCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctc                                                                                                                                                >  2:37190‑M2/1‑143 (MQ=255)          cAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTAAGGGTATTGTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTTGCTGAAGGCCACGGACCCGGGCCACCACCAGGTAATGCAGAGccttctccca                                                                                                                                             <  2:956587‑M2/150‑11 (MQ=255)             gCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaac                                                                                                                                          >  1:227005‑M2/1‑137 (MQ=255)              cAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaacc                                                                                                                                         <  2:55922‑M2/150‑15 (MQ=255)                  ttatttGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaagg                                                                                                                                     >  1:120038‑M2/1‑132 (MQ=255)                          cGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgag                                                                                                                             <  2:830964‑M2/150‑27 (MQ=255)                                   cTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttg                                                                                                                    >  2:780773‑M2/1‑115 (MQ=255)                                   cTTTGCCAGATCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttg                                                                                                                    >  2:488474‑M2/1‑115 (MQ=255)                                         cAGTTCCGGGTATTCTTCCTCATAAAAATCGGCACCATTGCATTTCAGCCAGCCTGTTTGCGGAGTGGATGAAGGCCACGGAACCGGTACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACa                                                                                                              <  2:93631/150‑1 (MQ=255)                                                     ttcttcAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgattt                                                                                                  >  2:758783‑M2/1‑97 (MQ=255)                                                       cttcAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGCGccttctcccaaacaaaggttttcgagcgccgtttgcacagtgccatccgatttga                                                                                                <  2:366603‑M2/150‑56 (MQ=255)                                                       cttcAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttga                                                                                                <  2:362196‑M2/150‑56 (MQ=255)                                                       cttcAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTGGGCGGAGTGGCTGAAGGCCTCGGAACCGGGACACCAACAGGTAATGCAGCGccgtctccctacccatgggtgacgagagcgggttgcacactgccatccgagttgc                                                                                                <  2:448811‑M2/150‑56 (MQ=255)                                                       ctgcAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCAACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttga                                                                                                <  2:155264‑M2/150‑56 (MQ=255)                                                        ttcAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaagggtttcgagagccgtttgcacagtgccatccgatttgat                                                                                               >  2:761986‑M2/1‑94 (MQ=255)                                                            gcagAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcg                                                                                           >  2:805044‑M2/1‑90 (MQ=255)                                                            gcagAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcg                                                                                           >  2:685105‑M2/1‑90 (MQ=255)                                                            gcagAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGATAATGAAGAAATAACAAGCAAATGGCATCATTCCt                                                                                           >  2:777539/1‑150 (MQ=255)                                                              agAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGCACCGGGACACCAACAGGTAATGCAGAGccttctccccaaccaaggttttcgagagccgtttgcacagtgccatccgatgtgatatcgcc                                                                                         <  1:65463‑M2/150‑63 (MQ=255)                                                              agAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaacccaaggttttcgagcgccggttgcacagtgccatccgagttgatatcgcc                                                                                         <  1:78126‑M2/150‑63 (MQ=255)                                                              agAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgcc                                                                                         <  2:744114‑M2/150‑63 (MQ=255)                                                                      cGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggat                                                                                 <  2:803216‑M2/150‑71 (MQ=255)                                                                       ggAACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCAAGGAACCGGGACACCAACAGGTAATGCAGCGccatatcacaaaccaagggttgcgagagccgtttgcaaagtgccatccgattttatatcgcaaaacggatt                                                                                <  2:354881‑M2/150‑72 (MQ=255)                                                                         gagcattGCATTTCAGCAAGCCTGTTGGGGGAGTTGCTGAAGGCCAAGGAACCGGGAAACCATCAGGTAATGCAGAGcattctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattct                                                                              >  1:642782‑M2/4‑77 (MQ=255)                                                                           ccATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGCACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCATACCACCGTTTATGAAATTGAAGAAATAACAAGCAATTGGCATCATTCCTGCTTTTACCAggcgg                                                                            >  1:786796/1‑150 (MQ=255)                                                                           ccATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttg                                                                            >  2:908911‑M2/1‑75 (MQ=255)                                                                              ttGCATTTCATCCAGCCTGTTGGGGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttatcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcgg                                                                         >  1:919948‑M2/1‑72 (MQ=255)                                                                                ggattTCAGCCAGCCTGTTGGCGGTGTGGCTGAAGGCCACGGAACCGGGAGACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggct                                                                       >  1:666448‑M2/3‑70 (MQ=255)                                                                                  aTTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggctta                                                                     <  1:172185‑M2/150‑83 (MQ=255)                                                                                   tttCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaa                                                                    >  2:305078‑M2/1‑67 (MQ=255)                                                                                      cagccagcCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacag                                                                 <  1:295649‑M2/150‑87 (MQ=255)                                                                                      cagccagcCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacag                                                                 <  1:395024‑M2/150‑87 (MQ=255)                                                                                      cagccagcCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacag                                                                 >  1:692569‑M2/1‑64 (MQ=255)                                                                                         ccagccGGTTGGCGTAGGGGCGGAAGGCCACGGAACCGGGACCCCAACAGGTAATGCAGACccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatcagatttgatatcgccaaacggattcttgcggcttaacagcag                                                              >  1:628329‑M2/1‑61 (MQ=255)                                                                                               tGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTATTGCTGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccttccgatttgatatcgcctatcggattcttgcggcttaacagcagcgggcg                                                        <  1:842711‑M2/150‑96 (MQ=255)                                                                                                       gAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcgg                                                >  2:821819‑M2/1‑47 (MQ=255)                                                                                                           ggCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaag                                            <  1:737985‑M2/150‑108 (MQ=255)                                                                                                              tGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaagtatttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaa                                         >  2:20949‑M2/1‑40 (MQ=255)                                                                                                                 aGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactg                                      >  1:835172‑M2/1‑37 (MQ=255)                                                                                                                 aGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttatcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactg                                      >  1:501902‑M2/1‑37 (MQ=255)                                                                                                                  ggCCACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttatcgagagccgtttgcacagtgccatccgaattgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggttagtaacttg                                     <  1:549327‑M2/150‑115 (MQ=255)                                                                                                                    ccACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtc                                   >  2:907901‑M2/1‑34 (MQ=255)                                                                                                                     cACGGAACCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcg                                  <  1:210815‑M2/150‑118 (MQ=255)                                                                                                                     cACGGAACCGGGACACCAACAGGTAATGCAGAGccttctccaaaaccaaggttttcgatagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgctgcttaacagcagcgcgcgaagtgcggtaagtaactggtcg                                  >  1:496799‑M2/1‑33 (MQ=255)                                                                                                                          aaCCGGGACACCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtatgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccg                             >  1:636369‑M2/1‑28 (MQ=255)                                                                                                                               ggACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAAcaca                        <  1:281132/150‑1 (MQ=255)                                                                                                                                   acCAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccgccccttctc                    >  2:172185‑M2/1‑19 (MQ=255)                                                                                                                                   acCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATc                    <  2:256862/150‑1 (MQ=255)                                                                                                                                    ccAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccgccccttctcc                   <  2:836681‑M2/150‑133 (MQ=255)                                                                                                                                    ccAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccgccccttctcc                   <  2:255243‑M2/150‑133 (MQ=255)                                                                                                                                     cAACAGGTAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccgccccttctcca                  <  2:162800‑M2/150‑134 (MQ=255)                                                                                                                                            tAATGCAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccgccccttctccaggctggc           >  2:579315‑M2/1‑10 (MQ=255)                                                                                                                                                 cAGAGccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccgccccttctccaggctggcaccgg      <  2:383207‑M2/150‑146 (MQ=255)                                                                                                                                                    agccttctcccaaaccaaggttttcgagagccggttgcacagtgccatccgagttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccgccccttctccaggctggcaccggatg   >  1:55922‑M2/1‑2 (MQ=255)                                                                                                                                                     gccttctcccaaaccaaggttttcgagagccgtttgcacagtgccatccgatttgatatcgccaaacggattcttgcggcttaacagcagcgcgcgaagtgcggtaagtaactggtcgtgccgccccttctcaggctggcaccggatgcc  <  1:908537‑M2/150‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CACGTAAATCAGGCAATTTATTTGTCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGA  >  NC_000913_3_bme_pgi/1209454‑1209751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.