New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 2662574	NA (NA)	8 (0.270) +CTACAGTGAACAGAACCGTAGGTCGGATAAGGCGTTCACGCCGCATCCG	4/188	4.3	NA	noncoding (266/266 nt)	REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences	REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences
	?	NC_000913_3_bme_pgi	= 2662583	NA (NA)					coding (741/741 nt)	trmJ	tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.

GTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAAC  >  NC_000913_3_bme_pgi/2662440‑2662731                                                                                                                                                |                                                                                                                                                     gTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTc                                                                                                                                                 >  1:509143/1‑149 (MQ=35)  ttCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGGTGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCg                                                                                                                                                <  1:654269/149‑1 (MQ=25)    cACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGc                                                                                                                                              <  2:414372/149‑1 (MQ=35)     aCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGcc                                                                                                                                             >  1:236421/1‑149 (MQ=37)         tAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGCCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACCCGTCCTATCAGGCCACGGCTGTTAATCGGCCttcg                                                                                                                                         >  2:341896/1‑147 (MQ=11)           ggCCTGATAAGACGCATTACGCGGCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTa                                                                                                                                       <  1:726841/149‑1 (MQ=255)             ccTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTAcc                                                                                                                                     <  1:336500/149‑1 (MQ=255)               tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCtt                                                                                                                                   >  1:147284/1‑149 (MQ=255)                 aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAAAGCCTGATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCtttg                                                                                                                                 >  1:353764/1‑148 (MQ=255)                 aTAAGACGCATTACGCCTCGCATCAGGCAACGGCTGCCGGATGCGGCATCACTCCCTTATCCGACCTACGGTTCTGTTCCCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTa                                                                                                                                 <  1:220788/149‑1 (MQ=14)                      aCGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctg                                                                                                                            >  1:627745/1‑149 (MQ=255)                        gCATTACGCGTCGCATCAGGCAACGGCTGTCAGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgct                                                                                                                          >  1:565046/1‑149 (MQ=255)                         cATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgctg                                                                                                                         <  1:667581/149‑1 (MQ=255)                          aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtgctgc                                                                                                                        >  2:647826/1‑149 (MQ=255)                                cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTAACTGATCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATa                                                                                                                  <  1:541442/149‑1 (MQ=255)                                   cgccgacggCAACGGCCGGCGGGTGCGGGGTGAACGCCTTACCCGACCTATGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                                                                               <  2:36578/142‑1 (MQ=18)                                      aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGcc                                                                                                            >  1:145000/1‑149 (MQ=255)                                       tCAGGCAATGGCTGGCGGATGCGGCGTGGACGCCTTATCCGTCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa                                                                                                           <  2:333043/149‑1 (MQ=255)                                       tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa                                                                                                           >  2:456367/1‑149 (MQ=255)                                            cAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                                                                               <  2:250790/140‑1 (MQ=255)                                            cAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                                                                               >  1:250790/1‑140 (MQ=255)                                              ccgtaggTCGGATAAGGCGTTCACGCCGCATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATccc                                                                                                    <  1:159452/143‑1 (MQ=21)                                                 gCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcgcg                                                                                                 <  2:230160/149‑1 (MQ=255)                                                 gCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcgcg                                                                                                 <  2:147284/149‑1 (MQ=255)                                                      cGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGa                                                                                            <  2:678099/149‑1 (MQ=255)                                                             ggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCaa                                                                                     <  1:434496/149‑1 (MQ=255)                                                             ggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTCTTACCTTTATTCTGCTGCTCATTACAAGCCAGAATCCCCCACACGATATTcca                                                                                     >  2:141277/1‑147 (MQ=18)                                                              gcgTGAACGCCTTATCCCACCTACGGTTCTGTTCCGTGTTGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAAc                                                                                    <  2:430879/149‑1 (MQ=255)                                                                  gAACGCATTATCCGACCTACGGTTCTGTTCACTGTAGTCCTTATAAGACGCATTACTCTTCGCATCAGGCAACGTCTGTTATTCGGCCTTGTTACCTTTATTCTTCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCt                                                                                <  2:221351/149‑1 (MQ=25)                                                                    aCGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTgg                                                                              <  1:67199/149‑1 (MQ=255)                                                                          tATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTcc                                                                        <  1:749187/149‑1 (MQ=255)                                                                            tCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCCCGCAGGATATTCAACTCCTGGCTTTCCgg                                                                      >  2:274291/1‑149 (MQ=255)                                                                               gACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTc                                                                                       <  2:524211/129‑1 (MQ=255)                                                                               gACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTc                                                                                       >  1:524211/1‑129 (MQ=255)                                                                               gACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcg                                                                   >  2:534497/1‑149 (MQ=255)                                                                                      ggTTTTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGTAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACgg                                                            <  1:177327/149‑1 (MQ=255)                                                                                      ggTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACgg                                                            <  2:627745/149‑1 (MQ=255)                                                                                            gTTCACTGTAGGCCTGATAAGACGCATTACGCGCCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAAc                                                      >  1:669985/1‑149 (MQ=255)                                                                                             ttCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGcc                                                                                                            <  1:650334/94‑1 (MQ=255)                                                                                             ttCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGcc                                                                                                            >  2:650334/1‑94 (MQ=255)                                                                                               cACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGa                                                   <  1:494470/149‑1 (MQ=255)                                                                                               cACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGa                                                   >  1:99244/1‑149 (MQ=255)                                                                                                aCTGTAGGTCTGATAGGACGCATTCCGTGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTCCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGAc                                                  <  1:494353/149‑1 (MQ=39)                                                                                                   gTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGgc                                               <  2:236421/149‑1 (MQ=255)                                                                                                    tAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGgcg                                              >  1:508943/1‑149 (MQ=255)                                                                                                           gATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTg                                                                                                                                          <  2:139412/50‑1 (MQ=255)                                                                                                           gATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTg                                                                                                                                          >  1:139412/1‑50 (MQ=255)                                                                                                            aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTAt                                      >  1:257043/1‑149 (MQ=255)                                                                                                            aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTAt                                      <  1:43024/149‑1 (MQ=255)                                                                                                              aaGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATtc                                    <  1:292156/149‑1 (MQ=255)                                                                                                                                       cAACGGTTGTTATTAGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAc           <  1:326837/149‑1 (MQ=255)                                                                                                                                       cAACGGCTGTTATTCGTCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATTTCCTGCCCCGGATGGTTTTCAc           <  2:91701/149‑1 (MQ=255)                                                                                                                                       cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAc           <  2:145000/149‑1 (MQ=255)                                                                                                                                       cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTAAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCAc           <  2:634071/149‑1 (MQ=255)                                                                                                                                            gCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGCATGGTTTTCACGGAtg      >  2:442601/1‑148 (MQ=255)                                                                                                                                                ttATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAAc  <  2:669985/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTAAACAGACGGCGCAATTTATTCATCACCTGCCCCGGATGGTTTTCACGGATAAAAC  >  NC_000913_3_bme_pgi/2662440‑2662731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.