New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	3853662 =	NA (NA)	6 (0.150)	6/262	4.9	NA	intergenic (+20/‑260)	tisB/emrD	toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
	?	NC_000913_3_bme_pgi	3853690 =	NA (NA)					intergenic (+48/‑232)	tisB/emrD	toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
Rejected: Coverage evenness skew score above cutoff.

GTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCC  >  NC_000913_3_bme_pgi/3853516‑3853808                                                                                                                                                   |                                                                                                                                                   gTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCaaa                                                                                                                                                  >  1:719861/1‑149 (MQ=255)  gcAGCCGGCAGTTCCCCTGAACCACAGGAGACGCGTATGAACCTTGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAg                                                                                                                                                 <  1:31202/148‑1 (MQ=255)           aGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCggg                                                                                                                                        >  2:756827/1‑149 (MQ=255)             tGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGaa                                                                                                                                      >  1:505255/1‑149 (MQ=255)                  cTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGc                                                                                                                                 <  1:756827/149‑1 (MQ=255)                        cacaGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCGAAGGGGAGCGGGAAACCGCTCCCCt                                                                                                                           >  1:344520/1‑149 (MQ=255)                        cacaGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCt                                                                                                                           >  1:159738/1‑149 (MQ=255)                        cacaGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCt                                                                                                                           >  1:662157/1‑149 (MQ=255)                                  gcgTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTa                                                                                                                 <  2:499161/149‑1 (MQ=255)                                      aTGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCGGATTCAAGTCGCACCAAAGGGGAGCGGGAAGCCGCTCCCCTTTCATATTTAGCGt                                                                                                             >  2:35766/1‑149 (MQ=255)                                      aTGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTGGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAGACCGCTCCCCGTTTATATTTAggtt                                                                                                             >  1:27566/1‑146 (MQ=255)                                            cTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGc                                                                                                                                           <  1:231935/113‑1 (MQ=255)                                            cTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGc                                                                                                                                           >  2:231935/1‑113 (MQ=255)                                            cTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGt                                                                                                       >  1:427385/1‑149 (MQ=255)                                              ggtggATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACATGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTg                                                                                                     <  2:465326/149‑1 (MQ=255)                                              ggtggATATCGACATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATTTCTGAAGTAAATCAGACGCCAATCGCAGCGATCGGGAGCGGGATTCCGCTTCTTTTTTTTATTGAGCGTGCGGGTTg                                                                                                     <  2:356414/149‑1 (MQ=255)                                               gtggATATCGCCATTCTTATCCTCACACCCAATGGAGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTgg                                                                                                    >  2:565503/1‑149 (MQ=255)                                               gtggATATCGCCATTATTATCCTCAAACTCATTGTTGCAGCGATGAAAAGTCGTGGTGCTGATCAGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATGTTTAGCGTGCGGGTTgg                                                                                                    >  1:581006/1‑149 (MQ=255)                                                       cGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgat                                                                                            <  1:428151/149‑1 (MQ=255)                                                       cGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgat                                                                                            <  1:428162/149‑1 (MQ=255)                                                        gCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgatg                                                                                           >  1:593744/1‑149 (MQ=255)                                                            ttCTTAGCCTCCAACTCCTTGTTGCCGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgatgcgat                                                                                       <  1:443513/149‑1 (MQ=255)                                                               ttATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCt                                                                                    <  1:34086/149‑1 (MQ=255)                                                               ttATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCt                                                                                    >  2:425416/1‑149 (MQ=255)                                                                  tCCTCAAGATCAGTGTTGAAGCCCGTCAACTGCTTGATGTTGTTCTGAAATATCTTAAGTTATTCAGATTCAAGTCGCACCAATGGGGATCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGAc                                                                                 <  2:166713/149‑1 (MQ=255)                                                                     tCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGGTGACGCa                                                                              >  1:58025/1‑149 (MQ=255)                                                                     tCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCa                                                                              >  2:459543/1‑149 (MQ=255)                                                                             aTTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCAGCTTATcc                                                                      >  1:208771/1‑149 (MQ=255)                                                                             aTTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTCTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCCTCTTATcc                                                                      >  2:698610/1‑149 (MQ=255)                                                                               tgtcgCAGCACTGCAACTGCTTTACGCTGTTCGGAAATTCCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGGTGCGATGCTGACGCATCTTGTTCGc                                                                    <  2:492359/149‑1 (MQ=255)                                                                                     agcaCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTAcc                                                              >  2:537933/1‑149 (MQ=255)                                                                                       caCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTCCCAt                                                            >  2:456829/1‑149 (MQ=255)                                                                                        aCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTg                                                                                                     >  2:89505/1‑107 (MQ=255)                                                                                        aCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTg                                                                                                     <  1:89505/107‑1 (MQ=255)                                                                                                   ttGATGCTGTTATGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTCCCATCTCTCCCGGCaa                                                >  1:483980/1‑149 (MQ=255)                                                                                                       tGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCACCAtt                                            >  1:671916/1‑149 (MQ=255)                                                                                                        gCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACAttt                                           >  2:613886/1‑149 (MQ=255)                                                                                                            ttCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTg                                       >  2:502585/1‑149 (MQ=255)                                                                                                                 aaaTACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCt                                  >  1:520258/1‑149 (MQ=255)                                                                                                                  aaTACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCtt                                 >  2:613536/1‑149 (MQ=255)                                                                                                                      ccTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGCTCCCGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTAATTGCCGCTTTTGt                             >  1:382069/1‑149 (MQ=255)                                                                                                                       cTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGtt                            >  1:299251/1‑149 (MQ=255)                                                                                                                        tGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGCGTTGGTGTCGGATGCGATGCTGACGCATCTTATCAGCCCTACCAACCCTCCCGGCAACACTCATTGCCCCttaactcc                           >  2:319035/1‑143 (MQ=255)                                                                                                                         gAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTa                          >  1:656766/1‑149 (MQ=255)                                                                                                                          aaGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACAAAATACTGCGGCAACATTTATTGCCGCTTTTGTTTAc                         <  1:776529/149‑1 (MQ=255)                                                                                                                               aTTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGTATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATAtt                    >  2:482675/1‑149 (MQ=255)                                                                                                                               aTTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATAtt                    <  2:299251/149‑1 (MQ=255)                                                                                                                                 tCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACCtcttct                  >  1:688390/1‑149 (MQ=255)                                                                                                                                     aTTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGATGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCg              >  1:759357/1‑149 (MQ=255)                                                                                                                                     aTTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCACTTTTATATTTAGCGTGCGGGTTGATGTCGGATGCGATGCTGACGCATCGTATCAGCCCTACCATCACCCCAGGCACCATTTATTGCCGCTCTTGTGTACATGTTATACCg              >  2:332383/1‑149 (MQ=255)                                                                                                                                       tCAAGTCGCACCAAAGGGGAGCGGGAACACGCCCCCCGTTAGTAGTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCt            <  1:345843/149‑1 (MQ=255)                                                                                                                                         aaGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTaa          >  1:228784/1‑149 (MQ=255)                                                                                                                                          aGTCGCACCAAAGGGGAGCGGGAAACCGCTTCTCTTTTATAATTCACAGTCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTaaa         <  1:574480/149‑1 (MQ=255)                                                                                                                                          aGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGCCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTaaa         >  1:327675/1‑149 (MQ=255)                                                                                                                                            tCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATATTACCCCCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACAGTTTCTGCCGCTAAACa       >  2:279664/1‑149 (MQ=255)                                                                                                                                               cACCAAAGGTGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAAtt    <  1:290050/149‑1 (MQ=255)                                                                                                                                                 ccAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTcc  <  2:520258/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCC  >  NC_000913_3_bme_pgi/3853516‑3853808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.