Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913_3_bme_pgi | 4,233,793 | C→A | intergenic (‑560/‑2114) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913_3_bme_pgi | 4,233,793 | 0 | C | A | 97.6% | 127.2 / NA | 42 | intergenic (‑560/‑2114) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (20/21); total (20/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAACGACGA > NC_000913_3_bme_pgi/4233652‑4233936 | tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGc > 1:216687/1‑149 (MQ=255) tttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGc < 1:249009/149‑1 (MQ=255) ttACACTCAACATTACGCTAACGGCACTAAAACCATCTCATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGGTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCt < 2:278878/149‑1 (MQ=255) aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGc > 1:601943/1‑149 (MQ=255) aCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGTAAGAGCCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCa > 1:78485/1‑149 (MQ=255) aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCAtt < 1:451314/149‑1 (MQ=255) ttACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTg < 2:533286/149‑1 (MQ=255) gCTAACGGCACTAAAACCATCACCTTTGTCTGTGACTGGCGCTACAATCGTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCg < 1:26749/149‑1 (MQ=255) gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGCAGAGACTCGATATCTGCAAGAGACGCGCTTAAGCTTGAAGCAACGGTAGCAAGGCTTAAAAAGCATTGGGCg < 2:493753/149‑1 (MQ=255) cTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCgg > 1:349913/1‑149 (MQ=255) aCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAAt > 2:376484/1‑149 (MQ=255) cggctgtAATACCATCACATTTTGTTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAATATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGGTTGAAGCAATGGTAGCAAGGCTGAAAAAGCATTGGGGGGAATc < 2:506854/143‑1 (MQ=255) cACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGc > 2:580950/1‑124 (MQ=255) cACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGc < 1:580950/124‑1 (MQ=255) tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc > 1:3977/1‑149 (MQ=255) tttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc > 1:287238/1‑149 (MQ=255) gACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGAAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCACGATAGGCGCACGGCATTc > 2:155949/1‑149 (MQ=255) cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGACACAACGGTAGCAAAGCTGAAAAAGCATTGGGCGAAATCGGCCCCCCGCGATATGACCGCGGCATTCAACGcctg > 2:639151/1‑147 (MQ=255) cTACAATCTTCCAAAGTCACAATTCTCAAAATAAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCCTTGGGCGGAATCGGCCCCGCGCGACATGCGCGCGGCGTGCAGCGCCGa > 1:525935/1‑149 (MQ=255) aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGAcccc > 1:64734/1‑149 (MQ=255) cTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCccggc < 1:133769/149‑1 (MQ=255) aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGtt > 2:394008/1‑149 (MQ=255) tCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCggg < 1:356239/149‑1 (MQ=255) acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCg > 2:258130/1‑149 (MQ=255) acaATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCGGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGCCCCCGCCCCATTCGGCCg > 2:326495/1‑149 (MQ=255) caATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGa < 2:349913/149‑1 (MQ=255) tcAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCg > 1:643893/1‑149 (MQ=255) aaaaTCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGct < 2:287238/149‑1 (MQ=255) aaTCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGctct < 2:189564/149‑1 (MQ=255) tCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGc < 2:430521/149‑1 (MQ=255) gaagaGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTg > 1:209376/1‑149 (MQ=255) cTAAGGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGAtcttct < 1:40467/149‑1 (MQ=255) tGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTc < 1:376484/149‑1 (MQ=255) aaGAGACGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACt > 2:297442/1‑149 (MQ=255) gagaCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATATTCTGTTCGACTgg < 2:64734/149‑1 (MQ=255) agaCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGt < 2:3977/149‑1 (MQ=255) agaCGCGACGAAGCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGt < 1:394008/149‑1 (MQ=255) gcgAAGAAGCTTGAAGCAACGGAACCAAAGCTAAAAAAGCAGTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa < 2:589808/149‑1 (MQ=255) gcaacgaACGTTGAAGCCTCGGTCCCAAAGCTGCAAAAGCATTGGGCGTAATCTGCCCCGCGCGATATGCGCGCGGCTGTCAGCGCCGACCCTGGTCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGaa < 2:482268/146‑1 (MQ=255) gCTTGAAGCAACGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGGAGAGCgggg > 2:657091/1‑149 (MQ=255) cTTGAAGCAACGGTAGCAAAACTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCAATCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGGTCAACTGGTCGAAGTGCCGGGt > 2:566218/1‑149 (MQ=255) aCGGTAGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAAcgacga < 2:643236/149‑1 (MQ=255) | TTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCGCGGCATTCAGCGCCGACCCCGGCCGGTTCGGGCGATATTCGCTCTGCCTGGATGATCTTCTGTTCGACTGGTCGAAGTGCCGGGTTAACGACGA > NC_000913_3_bme_pgi/4233652‑4233936 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |