Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	3,486,574	C→A	A152E (GCA→GAA)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	3,486,574	0	C	A	100.0%	107.3 / NA	36	A152E (GCA→GAA)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/18);  total (18/18)

GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCAC  >  NC_000913_3_bme_pgi/3486430‑3486717                                                                                                                                                 |                                                                                                                                                gctaaTTGGTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGACACTTCACAGAATGTGGGCAACCTGGCGTTCCTCGACGTGACCGGCCGCATTGCACAGACTCTGCTGAATCTGGAaaaa                                                                                                                                             <  2:437051/146‑1 (MQ=255)   cAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAaaca                                                                                                                                           >  2:109629/1‑149 (MQ=255)     aTTGATTCAGGTAAACCCGCACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGCATCTGGAAAaacaac                                                                                                                                         >  2:35497/1‑149 (MQ=255)         aTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGa                                                                                                                                     <  1:109629/149‑1 (MQ=255)          ttCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCAGAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAgca                                                                                                                                    >  2:462933/1‑147 (MQ=255)               gTAAACCCGGACATTCTGATGCGTTTCTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAGGTGGGCCACCTGGCGTTCCTCGACGTCACGGACCGCCTTCCACAGACCCTGCTGAATCTGCAACAACAACACGACGCtct                                                                                                                               >  2:233769/1‑147 (MQ=255)                       ggACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCAcc                                                                                                                       <  1:263211/149‑1 (MQ=255)                        gACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCAccc                                                                                                                      <  2:287134/149‑1 (MQ=255)                           aTTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATTACTCACCCGGa                                                                                                                   >  2:230638/1‑149 (MQ=255)                               tGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGt                                                                                                               <  2:149838/149‑1 (MQ=255)                               tGATGCGTTTGGTTGGACAGGTGGCGCGTCGTCTGCAAGGCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGt                                                                                                               <  1:245170/149‑1 (MQ=255)                                      tttaacTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCaaa                                                                                                        >  1:523030/6‑149 (MQ=255)                                          tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaa                                                                                                    <  1:324992/149‑1 (MQ=255)                                            tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa                                                                                                  <  2:295168/149‑1 (MQ=255)                                                      gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                        <  1:76534/149‑1 (MQ=255)                                                               cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTAt                                                                                                             <  2:39328/119‑1 (MQ=255)                                                               cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTAt                                                                                                             >  1:39328/1‑119 (MQ=255)                                                                tGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTTGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                              >  1:141242/1‑149 (MQ=255)                                                                   aaGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGCCAGGAAATTTGGCAg                                                                           >  1:311825/1‑149 (MQ=255)                                                                        gattCACAGAAAGTTGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCGGCTGAATCTGGACAAACAACCAGCCGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                                      <  1:230638/147‑1 (MQ=255)                                                                              gagaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTg                                                                >  1:308666/1‑149 (MQ=255)                                                                                                 tgTTCCTGGGCGTGGCGGGACGCATTGGAGAGATTGTGCTGGATCTGGAAAACCAATCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTTAGATTGTCGGCTGTTCTCGTGAAACCGTGGGa                                             <  2:251201/148‑1 (MQ=255)                                                                                                       tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAtt                                       >  2:443582/1‑149 (MQ=255)                                                                                                       tCGACGTGACGGGCCGCATTGAACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTAACCAGGACGGTATGCAAATGAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGAGACGCAtt                                       >  1:468240/1‑149 (MQ=255)                                                                                                               aCGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAAGCATTCtaacaat                               >  2:396657/1‑143 (MQ=255)                                                                                                                 gggCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCCTTCTGAAGAttc                             >  1:90029/1‑147 (MQ=255)                                                                                                                  ggCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt                            <  1:160288/149‑1 (MQ=255)                                                                                                                   gCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                           <  1:220170/149‑1 (MQ=255)                                                                                                                   gCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGGCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                           >  2:479355/1‑149 (MQ=255)                                                                                                                        ctggCACAGCCTCTGCGCAATCTGTAAAAACAACCAGTCGCTATGACTCACCCGGACGGTATGCAAATCAAAATTCCCCGTCAGGAAATTGTTCAGATTGTCGGCTGTTCTCGTGCAACCGTGGGACTCATTCTGAAGATGCTGGAAGa                      <  1:208647/146‑1 (MQ=255)                                                                                                                          tGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                    <  1:439253/149‑1 (MQ=255)                                                                                                                                     tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAcc              <  2:201002/144‑1 (MQ=255)                                                                                                                                     tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAcc              >  1:201002/1‑144 (MQ=255)                                                                                                                                     tgctgAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtc         >  2:289702/1‑149 (MQ=255)                                                                                                                                       cggAATCTGGAACAACCACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtctc       <  1:525139/147‑1 (MQ=255)                                                                                                                                            tCTGGAAAAACAACCAGACGCGATGACGCGCCCGGACGGGAGGCAAATCAAAATTACCCGGCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTGAGcac  >  2:261099/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                GCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCAC  >  NC_000913_3_bme_pgi/3486430‑3486717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑