Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi3,156,5910TG21.1% 57.6 / 19.1 38intergenic (+73/‑32)yqhD/dkgAaldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base T (12/18);  new base G (3/5);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-02
Rejected as consensus: Frequency below/above cutoff threshold.

ACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTA  >  NC_000913_3_bme_pgi/3156450‑3156736
                                                                                                                                             |                                                                                                                                                 
aCCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCgg                                                                                                                                            <  1:171453/149‑1 (MQ=255)
  ccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAGCACCTCACCGGAGCCTGCTCCGGTg                                                                                                                                          >  1:484259/1‑149 (MQ=255)
   cAACTGGGCGAAAATCCTGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGa                                                                                                                                         <  1:50699/149‑1 (MQ=255)
   cAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGa                                                                                                                                         <  1:240079/149‑1 (MQ=255)
   cAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGa                                                                                                                                         <  1:240621/149‑1 (MQ=255)
      cTGGGCGAAAATCCTGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGtt                                                                                                                                      <  1:195995/149‑1 (MQ=255)
            gAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTACGCTTTTTACGCCGCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTCAGGTCAtata                                                                                                                                >  2:463933/1‑149 (MQ=255)
            gAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGAAGCCTGCTCCGGTGAGtaaatacc                                                                                                                                >  2:266788/1‑142 (MQ=255)
              aaaTCATGACATTACGTTGGATGCCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATaaa                                                                                                                              <  1:125103/149‑1 (MQ=255)
              aaaTCATGACATTACGGTGGATTGCAGCCTCCGTATATACGAAGCCGCCCGCTAAGCTTTCTACGCCTCAAACTTTCGTTTTCGGGCATTGCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATaaa                                                                                                                              <  1:58466/149‑1 (MQ=255)
                      aCATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCCGAATTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAAc                                                                                                                      <  1:507295/149‑1 (MQ=255)
                            cggtGGATGTCAGCCGCCCGATCTACGAAGCCGGCCGCCAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTTCAGACTTAAGGTCACAGCACCTCACCGGGGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATgg                                                                                                                <  1:325325/146‑1 (MQ=255)
                                 taTGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAAt                                                                                                           <  2:401734/148‑1 (MQ=255)
                                   tGTCATCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAGCACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATcc                                                                                                         >  2:467492/1‑149 (MQ=255)
                                               tatataCGAGGCCGCCCGCTAAGGTTTTTACGCCTAAAATTTTCGTTTTCGGGCATTGCGTCCAGACTGAAGTTCACAACACCTCAACGGAGTCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTaa                                                                                             <  2:87166/149‑1 (MQ=255)
                                                    aCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTAc                                                                                        >  2:342373/1‑149 (MQ=255)
                                                          ccgcccgcTAACCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                                                                  <  2:31728/149‑1 (MQ=255)
                                                            gcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGc                                                                                <  1:331813/149‑1 (MQ=255)
                                                            gcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGc                                                                                <  1:331596/149‑1 (MQ=255)
                                                                  tAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAAGGTc                                                                          >  1:336236/1‑149 (MQ=255)
                                                                          ttACGACTCAAACTTTCGTTTTCCGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCa                                                                  <  1:90998/149‑1 (MQ=255)
                                                                                cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg                                                            >  1:277185/1‑149 (MQ=255)
                                                                                     accttCGTGTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTgg                                                       <  2:484259/146‑1 (MQ=255)
                                                                                                      tttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCGAGt                                      >  1:267644/1‑149 (MQ=255)
                                                                                                           tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                 <  1:518396/149‑1 (MQ=255)
                                                                                                           tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                 <  2:217068/149‑1 (MQ=255)
                                                                                                                  ttAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTa                          <  2:434034/149‑1 (MQ=255)
                                                                                                                       ttCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCAc                     >  1:323136/1‑149 (MQ=255)
                                                                                                                            aacgCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGcga                <  2:361414/149‑3 (MQ=255)
                                                                                                                            aacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCa                >  1:512015/1‑149 (MQ=255)
                                                                                                                            aacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCa                >  2:417583/1‑149 (MQ=255)
                                                                                                                                acTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCa            >  1:166105/2‑149 (MQ=255)
                                                                                                                                  tCACCGGAGCCTGCTCCGGTGAGTTAATATAAAGGAGGCACGTATGGCGAATCCAACCGTTATTAAGCTACAGCATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCcaa          >  1:240445/1‑149 (MQ=255)
                                                                                                                                  tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGaa                             <  2:162401/130‑1 (MQ=255)
                                                                                                                                  tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGaa                             >  1:162401/1‑130 (MQ=255)
                                                                                                                                    aCCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTAGGACTGGGCGTCTGGCAAGCAAGTAAAGAGGAAATCATCACCGCCAttacaaca        >  2:512091/1‑143 (MQ=255)
                                                                                                                                        gAGCCTGCTCGGGTGAGTTTATATAAAGGTCGAACGTATGGCCAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGt    <  2:225734/149‑1 (MQ=255)
                                                                                                                                          gCCTGCTCCGGTGAGTTCATATAAGGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAAGCACCGCCATTCAAAAAGCGTTa  <  2:145148/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
ACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTA  >  NC_000913_3_bme_pgi/3156450‑3156736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑