Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_bme_pgi	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_bme_pgi	1978503	1979278	776	27 [0]	[0] 27	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_bme_pgi	= 1978502	0 (0.000)	27 (0.940)	25/272	0.2	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_bme_pgi	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

CACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCC  >  NC_000913_3_bme_pgi/1978370‑1978642                                                                                                                                     |                                                                                                                                             cacTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttt                                                                                                                              <  1:498639‑M1/149‑17 (MQ=255)  acTTATCTAAGATTTTTCCTAAATTGAAGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCCATAATGCGTGATGCAGATCACACAAGACACTCAATTACTTAACATAAATGtataagtcatacttttg                                                                                                                             <  2:31094‑M1/149‑18 (MQ=255)         tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACTCACATACAACGGAGGGGGACTGCGAGTTTCAATAAGGCGTGATGGCGATCACACAAAACACTCAATTACTTACACTAAATGtataagtactacttttgttttggg                                                                                                                      >  2:187345‑M1/1‑125 (MQ=255)                     tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttggtttgggtgtatttcaatc                                                                                                          >  1:382534‑M1/1‑113 (MQ=255)                        aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCTATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                                                                       <  2:227851‑M1/149‑40 (MQ=255)                        aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                                                                       <  2:281457‑M1/149‑40 (MQ=255)                            aCGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaa                                                                                                   >  1:441180‑M1/1‑106 (MQ=255)                              gCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                 <  1:433851‑M1/149‑46 (MQ=255)                                  cTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                                                             <  1:205046‑M1/149‑50 (MQ=255)                                   tGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGaataagtcatacttttgttttcggtgtatttcaatctgctaaaaattttt                                                                                            >  2:95947‑M1/1‑99 (MQ=255)                                     tccccgtcaCTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                                                          <  2:159766‑M1/145‑53 (MQ=255)                                       cTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttttt                                                                                        >  2:367278‑M1/1‑95 (MQ=255)                                         cGTCACTACACGCACATACAACGGGCGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCATTTACTTAACATAATTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgcta                                                                                      <  2:179194‑M1/149‑57 (MQ=255)                                              cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACCAAAAAGTCAATTACTTAACATAAATGtataagtcatacttttgttttgggattatttcaatctgttaaaaagtttttcgctacgcta                                                                                 >  2:86045‑M1/1‑88 (MQ=255)                                              cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                 <  2:44271‑M1/149‑62 (MQ=255)                                                            aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                 <  1:274321‑M1/119‑46 (MQ=255)                                                            aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                 >  2:274321‑M1/1‑74 (MQ=255)                                                                        atgCGATTTTCAAGAATGCGTGATGCAGATCATACAAAACACTCAATTACATAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataat                                                       <  2:334505‑M1/148‑88 (MQ=255)                                                                                ttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgg                                               >  2:517823‑M1/1‑54 (MQ=255)                                                                                               tGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagt                                >  1:30167‑M1/1‑39 (MQ=255)                                                                                                   gATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatctaaatgggatgttgcgcacagtcaaa                            >  2:494488‑M1/1‑35 (MQ=255)                                                                                                                  tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaa             <  1:302599‑M1/149‑130 (MQ=255)                                                                                                                          ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatc     >  1:448185‑M1/1‑12 (MQ=255)                                                                                                                          ataACATAAATCtataagtcatacttttgtttggggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatc     <  2:208320‑M1/148‑138 (MQ=255)                                                                                                                            aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataat         <  1:456795‑M1/143‑134 (MQ=255)                                                                                                                            aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataat         >  2:456795‑M1/1‑10 (MQ=255)                                                                                                                             aCATAAATGtataagtcatacttttgttttgcgtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc  <  2:296290‑M1/149‑141 (MQ=255)                                                                                                                                     |                                                                                                                                             CACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCC  >  NC_000913_3_bme_pgi/1978370‑1978642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑