New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_bme_pgi = 37306532 (1.060)4 (0.150) 4/252 3.5 12.1% coding (145/951 nt) mhpF acetaldehyde‑CoA dehydrogenase II, NAD‑binding
?NC_000913_3_bme_pgi = 373047 30 (1.130)coding (127/951 nt) mhpF acetaldehyde‑CoA dehydrogenase II, NAD‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTATTTGATGCGACCAGCGCCGGTGCTCATGTGAAAAACGATGCCGCTTTACGCGA  >  NC_000913_3_bme_pgi/372922‑373195
                                                                                                                             |                                                                                                                                                    
cgAGGAAGGGTAAGGTCACCATTCTCGGGTCTGGTCACATGTGTACCGATCTGATGATTAAAATTGTGCGTCACGTTCAGCATCTGGAGCTGGCGGTGATGGGTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATg                                                                                                                               <  2:33927/148‑1 (MQ=255)
      aGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTc                                                                                                                         <  1:243931/149‑1 (MQ=255)
              cgcGCCAGTAGCGGTTCTTGCAACATGGGGACCGATCCGAGGATTAAAATATCGCGGCACGGTCAGCCGCTGGAGATGGCGGTGACGGTTGGCATTGATCCTCATTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCcaccac                                                                                                                 <  2:85208/147‑1 (MQ=255)
                  ccATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCAt                                                                                                             >  1:307410/1‑149 (MQ=255)
                   cATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATg                                                                                                            >  1:427931/1‑149 (MQ=255)
                        tCGGTTCTGGCAACACTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAggg                                                                                                       <  1:146654/149‑1 (MQ=255)
                            ttCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTAAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGa                                                                                                   >  1:79862/1‑149 (MQ=255)
                               tGGCAACAGTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATTGTTGGCATTGATCCTCAGTCCGACGGTCTGGCTCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCg                                                                                                <  1:301843/149‑1 (MQ=255)
                                          gTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGGTGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGGGATCGGACTGGTGAAc                                                                                     >  2:164149/1‑149 (MQ=255)
                                              cGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGGGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGc                                                                                 >  1:185326/1‑149 (MQ=255)
                                               gATCGGGTGATTAAAATGTTGCGTCAGGGGCAGCATGTGGAGATGGCGGGGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGcc                                                                                <  2:14647/149‑1 (MQ=255)
                                                 tCTGATGATTAAAATTTTCCGTCACGGTCAGCATCTGGCCATGGCGTCGATGCTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCAACCCCCCATGAAGGGGCGCTCGGACGGATGAAAATGCCTg                                                                              >  1:105888/1‑149 (MQ=255)
                                                   tgatgaTTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACTTGCCTGaa                                                                            >  2:287774/1‑149 (MQ=255)
                                                   tgatgaTTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGaa                                                                            >  1:175201/1‑149 (MQ=255)
                                                    gatgatTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCGCCACCCATTGAGGGGTGATCGGACTGATGAACATGCCTGAAt                                                                           >  1:189242/1‑149 (MQ=255)
                                                        atTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGc                                                                       <  1:245208/149‑1 (MQ=255)
                                                                           gTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTAttt                                                    >  1:313196/1‑149 (MQ=255)
                                                                                    tGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTa                                                       <  1:229933/137‑1 (MQ=255)
                                                                                    tGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTa                                                       >  2:229933/1‑137 (MQ=255)
                                                                                             cGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTATTTGATGCGACCAGCGCCGGt                                  <  1:312627/149‑1 (MQ=255)
                                                                                                          cATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGACGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTATTTGATGCGACCAGCGCCGGTGCTCATGTGaaaa                     >  1:227916/1‑149 (MQ=255)
                                                                                                           aTTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCAACCACCCATGAAGGGGAGGTCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTATTTGATGCGACCACCGCCGGTGCTCATCTGaaaaa                    >  1:83413/1‑149 (MQ=255)
                                                                                                           aTTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATCGGCGCCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATACCGACATTTTATTTGATGCGACCAACGCAGTTGCTCAAGTGaaaca                    >  2:409351/1‑147 (MQ=255)
                                                                                                              ggtCCTCAGTGCGGCGGTCTGGCGCCCGCCAGACGTATGGGCGTGGCAACCACCTATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCTACATTGTATTTGATGCGACCAGCGCCGGTGATCATGTGAAAAACGa                 <  2:427508/147‑1 (MQ=255)
                                                                                                              gATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGCATTTGATGCGACCAGCGCCGGTGCTCATGTGAAAAACGa                 <  2:427931/149‑1 (MQ=255)
                                                                                                                tCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTATTTGATGCGACCAGCGCCGGTGCTCATGTGAAAAACGATg               <  2:185326/149‑1 (MQ=255)
                                                                                                                tCCTCAGTCCGACGGTCCGGCGCGCGCCACACGTATGGGCGTCGCCACCACCCATCAAAGCGCAATCGGACTGATGAACATGCCCGCACTTCCTTCTATCGACATTGTATTTGATGCGACCAACGCCGGTCCACATGCGAAGAACGact               >  2:30567/1‑147 (MQ=255)
                                                                                                                         tgACGGGCGGGGGGGCGTCGGAGGTATGGGCGTCGCCGCCACCCATGAAGGGGTGATCGGACTGATGAACTTGCCTGAAGTCGCTGATATCGACATTGTATTTGATGCGACCAGCGCCGGTGCTCATGTGAAAAACGATGCCGCTTTAc      <  1:409351/148‑1 (MQ=255)
                                                                                                                         cGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTATTTGATGCGACCAGCGCCGGTGCTCATGTGAAAAACGATGCCGCTTTAc      <  2:175201/149‑1 (MQ=255)
                                                                                                                             ggTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTATTTGATGCGACCAGCGCCGGTGCTCAAGTGAAAAACGATGCCGCTGTAGgcga  >  1:513850/1‑149 (MQ=255)
                                                                                                                             |                                                                                                                                                    
TGAGTAAGCGTAAAGTCGCCATTATCGGTTCTGGCAACATTGGTACCGATCTGATGATTAAAATTTTGCGTCACGGTCAGCATCTGGAGATGGCGGTGATGGTTGGCATTGATCCTCAGTCCGACGGTCTGGCGCGCGCCAGACGTATGGGCGTCGCCACCACCCATGAAGGGGTGATCGGACTGATGAACATGCCTGAATTTGCTGATATCGACATTGTATTTGATGCGACCAGCGCCGGTGCTCATGTGAAAAACGATGCCGCTTTACGCGA  >  NC_000913_3_bme_pgi/372922‑373195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.