BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_bme_pgi	4,233,746	A→G	intergenic (‑513/‑2161)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_bme_pgi	4,233,746	0	A	G	100.0%	574.9 / NA	157	intergenic (‑513/‑2161)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (95/62); total (95/62)

Only 100 of 161 total aligned reads displayed.

GGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCG  >  NC_000913_3_bme_pgi/4233649‑4233842

gggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCagaa                                                                                                  >  1:1032804/1‑98 (MQ=255)

 ggTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAgaggag                                                                                               <  2:33917/100‑1 (MQ=255)

   tttACACTCAACATTACGCTAGCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTa                                                                                             >  2:4731002/1‑100 (MQ=255)

     tACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTAtt                                                                                           >  1:1117880/1‑100 (MQ=255)

     tACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTAtt                                                                                           >  1:3931605/1‑100 (MQ=255)

       cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCCGAGGAGTATTg                                                                                          >  2:1693294/1‑99 (MQ=255)

       cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTg                                                                                          >  2:1403702/1‑99 (MQ=255)

       cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGc                                                                                         <  2:1406821/100‑1 (MQ=255)

       cacTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGc                                                                                         >  1:3349261/1‑100 (MQ=255)

         ntCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCt                                                                                        >  2:2812627/2‑99 (MQ=255)

         cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCt                                                                                        >  1:1977663/1‑99 (MQ=255)

         cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCt                                                                                        >  2:2506976/1‑99 (MQ=255)

         cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTa                                                                                       <  1:102900/100‑1 (MQ=255)

           cAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAAt                                                                                     >  1:1554954/1‑100 (MQ=255)

           cAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTCCTAAt                                                                                     >  2:925057/1‑100 (MQ=255)

            aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATg                                                                                    >  1:70684/1‑100 (MQ=255)

              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAACTCAGAGGAGTATTGCTAATgg                                                                                   >  2:3212678/1‑99 (MQ=255)

              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATgg                                                                                   >  2:2579165/1‑99 (MQ=255)

              cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGc                                                                                  <  1:1896838/100‑1 (MQ=255)

                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATg                                                                                    <  2:2843242/93‑1 (MQ=255)

                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCaa                                                                                >  1:1864348/1‑97 (MQ=255)

                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCaa                                                                                >  1:1407835/1‑97 (MQ=255)

                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCaa                                                                                >  1:3762885/1‑97 (MQ=255)

                   cGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAaga                                                                              <  1:4540671/99‑1 (MQ=255)

                     cTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCCGAGGAGTATTGCTAATGGCAAgaga                                                                            >  2:3231621/1‑99 (MQ=255)

                       aaCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGAcgc                                                                         >  1:4233054/1‑100 (MQ=255)

                       aaCGGCACTAAAACCATCACATTATTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGc                                                                                         >  2:1631951/1‑84 (MQ=255)

                         cGGCACTAAAACCATCACATTTTTCTTTTTTTTGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGAcgc                                                                         >  2:3416615/1‑98 (MQ=255)

                         cGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAACATCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGcga                                                                       >  2:4395850/1‑100 (MQ=255)

                         cGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGcga                                                                       >  1:2569458/1‑100 (MQ=255)

                           gCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGcgacn                                                                     <  1:3260883/100‑2 (MQ=255)

                            cACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGcgacg                                                                     >  2:766463/1‑99 (MQ=255)

                            cACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGcgacg                                                                     >  2:376573/1‑99 (MQ=255)

                                 aaaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGc                                                                 <  2:4167369/98‑1 (MQ=255)

                                 aaaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCtt                                                               <  2:3915909/100‑1 (MQ=255)

                                 aaaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCtt                                                               <  2:1114602/100‑1 (MQ=255)

                                  aaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTg                                                              <  2:3336894/100‑1 (MQ=255)

                                    ccATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaa                                                            <  1:455421/100‑1 (MQ=255)

                                    ccATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGaa                                                            <  1:2523391/100‑1 (MQ=255)

                                     cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATGAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAg                                                           <  1:4750247/100‑1 (MQ=255)

                                      aTCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAg                                                           >  1:260189/1‑99 (MQ=255)

                                      aTCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGc                                                          <  2:1309250/100‑1 (MQ=255)

                                         acaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAAc                                                       >  1:1636872/1‑100 (MQ=255)

                                           aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACgg                                                     >  2:3405051/1‑100 (MQ=255)

                                           aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACgg                                                     <  1:377761/100‑1 (MQ=255)

                                               ttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCg                                                  <  1:2758742/99‑1 (MQ=255)

                                                 cTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCa                                                >  1:3670837/1‑99 (MQ=255)

                                                  tgtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCa                                                >  1:1186831/1‑98 (MQ=255)

                                                   gtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAg                                             >  2:3539552/1‑100 (MQ=255)

                                                   gtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAg                                             >  2:4133150/1‑100 (MQ=255)

                                                    tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGc                                            <  1:2587827/100‑1 (MQ=255)

                                                     gACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCt                                           >  2:2973908/1‑100 (MQ=255)

                                                     gACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCt                                           >  2:2973878/1‑100 (MQ=255)

                                                      aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGcn                                           <  2:510976/99‑2 (MQ=255)

                                                          gcgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTTCTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCAAAAAGCTAaaaa                                      <  2:4611300/100‑1 (MQ=255)

                                                          gcgcTACAATCTGCCAAAGTCGCAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCt                                           <  1:235591/95‑1 (MQ=255)

                                                           cgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGaaaaa                                     <  1:18119/100‑1 (MQ=255)

                                                            gcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                    >  1:2885426/1‑100 (MQ=255)

                                                             cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATAGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAg                                    >  1:4751018/1‑99 (MQ=255)

                                                             cTACAATCTTCCAAAGAAACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCAAAAAGATGAAAAAg                                    <  2:2187208/99‑1 (MQ=255)

                                                                cAATCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCAtt                                <  2:493919/100‑1 (MQ=255)

                                                                  aTCTTCCAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTgg                              <  1:985381/100‑1 (MQ=255)

                                                                       ccAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGc                            <  1:3417805/97‑1 (MQ=255)

                                                                        cAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGACGCAACGGTCGCAACGCt                                           >  2:3460158/1‑81 (MQ=255)

                                                                        cAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGaa                        <  2:2643397/100‑1 (MQ=255)

                                                                        cAAAGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCATGAAGCAACGGTCGCAAAGCTGAAAAAGCTTTGGGCgg                          >  2:4737976/1‑98 (MQ=255)

                                                                         aaaGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAAt                       >  2:4028633/1‑100 (MQ=255)

                                                                         aaaGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAAt                       <  1:398790/100‑1 (MQ=255)

                                                                          aaGTCATCATTTTCAAAATCAGAGTAGTATTGCTAATGGCAAGAGACGAGACGAAGCTTGAAGAGACGATCGAAAAGCTGGAAAAGCAt                                 >  2:3491522/1‑89 (MQ=255)

                                                                           aGTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCg                     <  2:2439795/100‑1 (MQ=255)

                                                                            gTCACAATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCgg                    <  2:3283978/100‑1 (MQ=255)

                                                                              cacaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                  >  2:2354276/1‑100 (MQ=255)

                                                                              cacaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                  >  1:84697/1‑100 (MQ=255)

                                                                              cacaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcc                  >  2:3155273/1‑100 (MQ=255)

                                                                              cacaATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGCAATCGGcc                  >  2:4512147/1‑100 (MQ=255)

                                                                                caATTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc                >  1:1710163/1‑100 (MQ=255)

                                                                                 aaTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGcccc                <  2:3340391/99‑1 (MQ=255)

                                                                                 aaTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcg               <  1:493422/100‑1 (MQ=255)

                                                                                  aTTCTCAAAATCAGAGGGGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcg               <  2:1579915/99‑1 (MQ=255)

                                                                                  aTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGccc                 <  2:4090969/97‑1 (MQ=255)

                                                                                  aTTCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgc              >  1:733717/1‑100 (MQ=255)

                                                                                   ttCTCAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcg             >  1:3850675/1‑100 (MQ=255)

                                                                                    tctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  1:2433265/1‑100 (MQ=255)

                                                                                    tctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  1:3529527/1‑100 (MQ=255)

                                                                                    tctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  2:1109788/1‑100 (MQ=255)

                                                                                     ctcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCcgcgc            >  2:2134029/1‑99 (MQ=255)

                                                                                      tcAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa          >  1:2835429/1‑100 (MQ=255)

                                                                                       cAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa          >  2:3282637/1‑99 (MQ=255)

                                                                                       cAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa          >  2:584798/1‑99 (MQ=255)

                                                                                       cAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa          >  2:3832127/1‑99 (MQ=255)

                                                                                       cAAAATCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCCGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGa          >  2:1045501/1‑99 (MQ=255)

                                                                                        aaaaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGata        <  2:4041885/100‑1 (MQ=255)

                                                                                         aaaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGatat       >  1:2787447/1‑100 (MQ=255)

                                                                                          aaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATg      >  1:2727065/1‑100 (MQ=255)

                                                                                          aaTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTAGAAAAGATGAAAAAGCATTGGGCgg                          <  2:2609958/80‑1 (MQ=255)

                                                                                           aTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgc     >  1:1707136/1‑100 (MQ=255)

                                                                                           aTCAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgc     <  2:808099/100‑1 (MQ=255)

                                                                                             cAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc   >  2:1713310/1‑100 (MQ=255)

                                                                                             cAGAGGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc   >  1:2740935/1‑100 (MQ=255)

                                                                                              agaggaGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATgcgc   >  2:196121/1‑99 (MQ=255)

GGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGCAAGAGACGCGACGAAGCTTGAAGCAACGGTCGCAAAGCTGAAAAAGCATTGGGCGGAATCGGCCCCGCGCGATATGCGCG  >  NC_000913_3_bme_pgi/4233649‑4233842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`