Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,318	G→C	R110G (CGT→GGT)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,318	0	G	C	97.0%	112.2 / ‑6.8	33	R110G (CGT→GGT)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (25/7);  total (26/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGG  >  NC_000913/1308170‑1308463                                                                                                                                                     |                                                                                                                                                  tCCGCCATGCCGCCGGGCTGCCAGATATAAAACCCCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTgaccac                                                                                                                                                 <  2:221513/151‑1 (MQ=255)        tGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCa                                                                                                                                          >  2:47152/1‑151 (MQ=255)               gggCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTg                                                                                                                                   >  1:307488/1‑151 (MQ=255)                         atatAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGGTCCCCTGACCACGCTCGCAAAGCTTGAATAATGgcg                                                                                                                         >  1:366030/1‑151 (MQ=255)                         atatAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATgg                                                                                                                           <  2:131704/149‑1 (MQ=255)                         atatAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGgcg                                                                                                                         >  1:345517/1‑151 (MQ=255)                         atatAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGgcg                                                                                                                         >  1:149261/1‑151 (MQ=255)                         aaataAAACACCATCTCAATATTTGGGCGCGCGATTTGGGTTTTGCGGATTAACACCTGCACCACACCACCTGACTCGGGTATCAGTTGTAGCTGATTTCCTTGGACTCCAGCGATTCCCTGACGCCGCTCCCAACGCTTaaaacatgcg                                                                                                                          >  1:156135/3‑143 (MQ=255)                          tataAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGgcg                                                                                                                         >  2:125053/1‑150 (MQ=255)                                  acCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGacac                                                                                                                <  2:172711/151‑1 (MQ=255)                                      tctcAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTg                                                                                                              >  1:305738/1‑149 (MQ=255)                                      tctcAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTg                                                                                                              >  2:63795/1‑149 (MQ=255)                                       ctcAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTa                                                                                                           >  2:93840/1‑151 (MQ=255)                                           atatTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTAtttt                                                                                                       >  2:113403/1‑151 (MQ=255)                                           atatTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTAtttt                                                                                                       >  2:381712/1‑151 (MQ=255)                                            tattatGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTttc                                                                                                      <  1:356024/151‑1 (MQ=255)                                            tattatGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTttc                                                                                                      <  2:342315/151‑1 (MQ=255)                                             attatGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTAtttt                                                                                                       >  1:374262/1‑149 (MQ=255)                                                                   tCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAgg                                                                               >  2:354432/1‑151 (MQ=255)                                                                        gATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTa                                                                          >  2:80207/1‑151 (MQ=255)                                                                        gATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTa                                                                          >  2:351276/1‑151 (MQ=255)                                                                         aTTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTAAAGGCTTTaa                                                                         >  2:291084/1‑151 (MQ=255)                                                                          ttAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTCCAGGCTTTAAg                                                                        >  2:190036/1‑151 (MQ=255)                                                                           tAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTGAAgg                                                                       <  1:6608/151‑1 (MQ=255)                                                                                   gCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAg                                                               >  2:132105/1‑151 (MQ=255)                                                                                   gCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAg                                                               >  2:343587/1‑151 (MQ=255)                                                                                          atcatcTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCAtttt                                                        <  1:78977/151‑1 (MQ=255)                                                                                            catcTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTg                                                       >  1:327757/1‑150 (MQ=255)                                                                                            catcTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTg                                                       >  1:359252/1‑150 (MQ=255)                                                                                                              gTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCg                                     >  1:267759/1‑150 (MQ=255)                                                                                                                                       aGCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTg           >  2:63605/1‑151 (MQ=255)                                                                                                                                        gCGATCCCCTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGAAACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTg           >  2:167561/1‑150 (MQ=255)                                                                                                                                              cccTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAAt    >  1:76657/1‑151 (MQ=255)                                                                                                                                                cTGACCACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATgg  <  1:221940/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TCCGCCATGCCGCCGGGCTGCCAGATATAAAACACCATCTCAATATTATGGCGCGCGAGCTGGATGTCGCGGATTAACGCCTGCATCACATCATCTGACTCGGTCATCAGTTGTAGCTGATTCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGG  >  NC_000913/1308170‑1308463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑