breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 10,637 C→A G18G (GGC→GGA mogA → molybdenum cofactor biosynthesis protein MogA
RA 194,161 A→G M42V (ATG→GTG)  LY180_00835 → 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase
RA 230,529 T→C D275G (GAC→GGC)  LY180_01015 ← LysR family transcripitonal regulator
RA 459,705 Δ1 bp coding (137/276 nt) LY180_02175 ← transcriptional regulator
RA 793,877 T→C C358R (TGT→CGT)  LY180_03720 → rhsA
RA 793,936 T→C S377S (AGT→AGC LY180_03720 → rhsA
RA 793,954 A→G E383E (GAA→GAG LY180_03720 → rhsA
RA 793,985 A→G N394D (AAC→GAC)  LY180_03720 → rhsA
RA 814,037 G→C T139S (ACC→AGC)  gltA ← type II citrate synthase
MC JC 1,011,041 Δ300 bp intergenic (+21/+62) LY180_04735 → / ← pflA hypothetical protein/pyruvate formate lyase‑activating protein
MC JC 1,088,034 Δ103 bp intergenic (+278/+242) helD → / ← LY180_05060 DNA helicase IV/hypothetical protein
RA 1,168,401 C→A S606* (TCG→TAG)  LY180_05445 → glucosyltransferase
RA 1,213,485 Δ7 bp intergenic (+184/‑105) LY180_05705 → / → LY180_05710 DNAse/PTS glucose transporter subunit IIBC
RA 1,324,434 A→C K833T (AAA→ACA)  narZ → nitrate reductase A subunit alpha
RA 1,335,968 A→G intergenic (+281/+21) LY180_06295 → / ← LY180_06300 thymidine kinase/bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
MC JC 1,335,989 2720 bp→18 bp [LY180_06300][LY180_06310] [LY180_06300], LY180_06305, [LY180_06310]
MC JC 1,446,672 Δ2,957 bp [zntB][LY180_06850] [zntB], LY180_06845, [LY180_06850]
RA 1,459,895 C→T T49T (ACC→ACT LY180_06935 → hypothetical protein
RA 1,464,821 A→G intergenic (+486/‑118) LY180_06955 → / → LY180_06960 hypothetical protein/regulatory protein
RA 1,505,743 2 bp→GT intergenic (‑91/+19) LY180_07195 ← / ← LY180_07200 heat‑inducible protein/hypothetical protein
RA 1,505,880 T→C L35L (CTA→CTG LY180_07200 ← hypothetical protein
RA 1,505,925 A→G I20I (ATT→ATC LY180_07200 ← hypothetical protein
RA 1,505,928 T→C V19V (GTA→GTG LY180_07200 ← hypothetical protein
RA 1,505,955 T→C L10L (CTA→CTG LY180_07200 ← hypothetical protein
RA 1,563,485 A→G T256A (ACC→GCC)  LY180_07445 → transposase IS609
RA 1,563,642 G→A R308H (CGC→CAC)  LY180_07445 → transposase IS609
RA 1,563,706 C→T Y329Y (TAC→TAT LY180_07445 → transposase IS609
RA 1,563,726 G→A C336Y (TGC→TAC)  LY180_07445 → transposase IS609
RA 1,563,748 A→G E343E (GAA→GAG LY180_07445 → transposase IS609
RA 1,563,751 T→C N344N (AAT→AAC LY180_07445 → transposase IS609
RA 1,588,241 A→C E403D (GAA→GAC LY180_07560 → type IV secretion protein Rhs
RA 1,645,301 T→G intergenic (+1095/+165) LY180_07765 → / ← LY180_07770 transposase/sulfatase
RA 1,645,397 C→T intergenic (+1191/+69) LY180_07765 → / ← LY180_07770 transposase/sulfatase
RA 1,645,421 C→G intergenic (+1215/+45) LY180_07765 → / ← LY180_07770 transposase/sulfatase
MC JC 1,749,406 Δ777 bp [LY180_08300][LY180_08310] [LY180_08300], LY180_08305, [LY180_08310]
JC 1,840,785 (AAAAACTTTGTGTAATACTTG)1→2 intergenic (+268/‑43) LY180_08735 → / → LY180_08740 pyruvate kinase/murein lipoprotein
RA 1,964,559 G→A N226N (AAC→AAT LY180_09345 ← transposase IS609
RA 1,964,699 C→T V180I (GTC→ATC)  LY180_09345 ← transposase IS609
RA 1,964,727 G→T S170S (TCC→TCA LY180_09345 ← transposase IS609
RA 1,964,784 A→C T151T (ACT→ACG LY180_09345 ← transposase IS609
RA 2,251,239 G→A P28P (CCC→CCT LY180_10740 ← hypothetical protein
RA 2,251,586 T→C E104E (GAA→GAG LY180_10745 ← major tail tube protein
RA 2,251,616 A→G R94R (CGT→CGC LY180_10745 ← major tail tube protein
RA 2,265,347 G→A intergenic (‑100/‑74) LY180_10855 ← / → LY180_10860 precorrin‑8W decarboxylase/terminase
RA 2,265,396 A→G intergenic (‑149/‑25) LY180_10855 ← / → LY180_10860 precorrin‑8W decarboxylase/terminase
RA 2,265,399 G→T intergenic (‑152/‑22) LY180_10855 ← / → LY180_10860 precorrin‑8W decarboxylase/terminase
RA 2,265,885 C→A A155A (GCC→GCA LY180_10860 → terminase
JC 2,274,707 +57 bp intergenic (‑200/+31) LY180_10890 ← / ← LY180_10895 hypothetical protein/hypothetical protein
RA 2,312,761 T→A intergenic (‑71/+448) LY180_11070 ← / ← LY180_11075 hypothetical protein/hypothetical protein
RA 2,312,767 3 bp→GAC intergenic (‑77/+440) LY180_11070 ← / ← LY180_11075 hypothetical protein/hypothetical protein
RA 2,312,771 G→A intergenic (‑81/+438) LY180_11070 ← / ← LY180_11075 hypothetical protein/hypothetical protein
RA 2,312,773 C→G intergenic (‑83/+436) LY180_11070 ← / ← LY180_11075 hypothetical protein/hypothetical protein
RA 2,483,647 G→A P236S (CCG→TCG)  LY180_11800 ← NADH:ubiquinone oxidoreductase subunit M
MC JC 2,505,263 Δ394 bp intergenic (‑620/‑500) LY180_11895 ← / → LY180_11905 hypothetical protein/phosphate acetyltransferase
RA 2,505,816 A→G intergenic (‑1173/‑340) LY180_11895 ← / → LY180_11905 hypothetical protein/phosphate acetyltransferase
RA 2,506,026 T→C intergenic (‑1383/‑130) LY180_11895 ← / → LY180_11905 hypothetical protein/phosphate acetyltransferase
MC JC 2,606,939 Δ122 bp LY180_12380 LY180_12380
RA 3,010,362 G→T E48* (GAA→TAA)  LY180_14330 → type VI secretion protein ImpK
RA 3,028,824 G→A L110L (CTG→CTA LY180_14390 → hypothetical protein
RA 3,028,828 G→A A112T (GCC→ACC)  LY180_14390 → hypothetical protein
RA 3,278,551 G→A W246* (TGG→TGA LY180_15575 → aldehyde oxidoreductase
MC JC 3,431,620 Δ68 bp coding (288‑355/879 nt) LY180_16320 → protease
RA 3,505,943 C→T G41D (GGT→GAT)  LY180_16680 ← malate dehydrogenase
RA 3,545,689 T→C noncoding (2811/2931 nt) LY180_16870 ← 23S ribosomal RNA
RA 3,545,692 C→T noncoding (2808/2931 nt) LY180_16870 ← 23S ribosomal RNA
RA 3,545,695 G→A noncoding (2805/2931 nt) LY180_16870 ← 23S ribosomal RNA
RA 3,545,697 A→G noncoding (2803/2931 nt) LY180_16870 ← 23S ribosomal RNA
RA 3,593,153 G→A G142D (GGC→GAC)  LY180_17220 → transcriptional regulator
JC 3,812,239 (AGA)2→4 coding (434/1020 nt) LY180_18170 ← peptide ABC transporter permease
RA 3,943,262 G→A G302D (GGC→GAC)  LY180_18795 → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 4,023,563 T→C L4P (CTC→CCC)  rnpA → ribonuclease P
RA 4,178,979 T→C noncoding (1009/1541 nt) LY180_19945 → 16S ribosomal RNA
RA 4,178,988 Δ1 bp noncoding (1018/1541 nt) LY180_19945 → 16S ribosomal RNA
RA 4,178,991 A→T noncoding (1021/1541 nt) LY180_19945 → 16S ribosomal RNA
RA 4,178,993 +G noncoding (1023/1541 nt) LY180_19945 → 16S ribosomal RNA
RA 4,179,007 C→T noncoding (1037/1541 nt) LY180_19945 → 16S ribosomal RNA
RA 4,305,124 G→A G42G (GGG→GGA LY180_20645 → rhsA
RA 4,305,151 G→C P51P (CCG→CCC LY180_20645 → rhsA
RA 4,305,201 C→G A68G (GCC→GGC)  LY180_20645 → rhsA
MC JC 4,394,727 Δ4,605 bp LY180_21020LY180_21030 LY180_21020, adhP, LY180_21030
RA 4,469,025 +A coding (748/1350 nt) LY180_21345 → permase
MC JC 4,815,129 Δ342 bp coding (402‑743/1224 nt) LY180_23005 → phosphopentomutase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP006584 1505961 1510560 4600 15 [14] [14] 15 [LY180_07200]–[LY180_07220] [LY180_07200],LY180_07205,LY180_07210,LY180_07215,[LY180_07220]
* * ÷ CP006584 1644423–1644851 1645276–1644891 41–854 15 [14] [13] 15 LY180_07765/LY180_07770 transposase/sulfatase
* * ÷ CP006584 3028904–3029236 3032122 2887–3219 15 [14] [14] 16 [LY180_14390]–LY180_14410 [LY180_14390],LY180_14395,LY180_14400,LY180_14405,LY180_14410
* * ÷ CP006584 3345694–3346065 3377350–3376399 30335–31657 15 [14] [14] 16 ebgC–LY180_16025 ebgC,LY180_15890,LY180_15895,LY180_15900,fadH,LY180_15910,LY180_15915,LY180_15920,LY180_15925,LY180_15930,LY180_15935,LY180_15940,LY180_15945,LY180_15950,LY180_15955,LY180_15960,LY180_15965,LY180_15970,LY180_15975,LY180_15980,LY180_15985,LY180_15990,LY180_15995,LY180_16000,LY180_16005,LY180_16010,LY180_16015,LY180_16020,LY180_16025

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP006584 5598 =NA (NA)85 (1.450) 72/562 0.0 100% intergenic (+69/‑191) LY180_00025/LY180_00030 hypothetical protein/hypothetical protein
?CP006584 3172592 = 0 (0.000)coding (29/273 nt) LY180_15050 hypothetical protein
* ? CP006584 = 225257NA (NA)75 (1.230) 68/584 0.1 NA noncoding (1014/1541 nt) LY180_00980 16S ribosomal RNA
?CP006584 225269 = NA (NA)noncoding (1026/1541 nt) LY180_00980 16S ribosomal RNA
* ? CP006584 = 26913068 (1.110)76 (1.320)
+TCATGTCATTAGTGCGTT
63/550 0.1 70.4% coding (377/414 nt) LY180_01180 hypothetical protein
?CP006584 1594499 = 0 (0.000)coding (168/909 nt) LY180_07575 hypothetical protein
* ? CP006584 294480 =40 (0.650)19 (0.350) 19/518 2.0 34.8% coding (26/996 nt) LY180_01335 hypothetical protein
?CP006584 297564 = 36 (0.660)coding (496/1515 nt) LY180_01350 tail protein
* ? CP006584 = 29451341 (0.670)21 (0.390) 21/518 1.7 36.8% coding (59/996 nt) LY180_01335 hypothetical protein
?CP006584 = 297529 36 (0.660)coding (531/1515 nt) LY180_01350 tail protein
* ? CP006584 346068 =NA (NA)73 (1.190) 59/586 0.1 96.0% intergenic (‑51/‑107) LY180_01675/LY180_01680 CadC family transcriptional regulator/hypothetical protein
?CP006584 = 363812 3 (0.050)intergenic (‑39/‑249) LY180_01770/LY180_01775 outer membrane phosphoporin protein E/gamma‑glutamyl kinase
* ? CP006584 363804 =3 (0.050)98 (1.600) 70/586 0.0 97.0% intergenic (‑31/‑257) LY180_01770/LY180_01775 outer membrane phosphoporin protein E/gamma‑glutamyl kinase
?CP006584 = 463123 NA (NA)coding (772/816 nt) LY180_02200 membrane protein
* ? CP006584 840689 =28 (0.460)25 (0.460) 24/524 1.4 50.5% intergenic (+68/‑82) LY180_03930/LY180_03935 tRNA‑Lys/tRNA‑Val
?CP006584 = 841381 24 (0.440)intergenic (+125/‑280) LY180_03950/LY180_03955 tRNA‑Lys/quinolinate synthetase
* ? CP006584 917080 =NA (NA)42 (0.690) 37/580 0.7 97.7% intergenic (+88/‑191) LY180_04320/LY180_04325 heme ABC transporter ATP‑binding protein/hypothetical protein
?CP006584 = 2414713 1 (0.020)coding (1017/1644 nt) LY180_11545 multidrug ABC transporter ATP‑binding protein
* ? CP006584 1015084 =1 (0.020)48 (0.780) 47/586 0.4 98.0% coding (445/858 nt) LY180_04750 formate transporter
?CP006584 = 1154738 NA (NA)coding (35/636 nt) LY180_05370 hydrolase
* ? CP006584 = 10150933 (0.050)52 (0.850) 46/584 0.4 94.6% coding (436/858 nt) LY180_04750 formate transporter
?CP006584 1153411 = NA (NA)intergenic (‑823/+12) pgaA/LY180_05365 poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein/hypothetical protein
* ? CP006584 1153410 =NA (NA)73 (1.190) 61/586 0.1 91.2% intergenic (‑822/+13) pgaA/LY180_05365 poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein/hypothetical protein
?CP006584 1213575 = 7 (0.110)intergenic (+274/‑21) LY180_05705/LY180_05710 DNAse/PTS glucose transporter subunit IIBC
* ? CP006584 = 12134846 (0.100)47 (1.110)
+90 bp
47/406 0.1 91.9% intergenic (+183/‑112) LY180_05705/LY180_05710 DNAse/PTS glucose transporter subunit IIBC
?CP006584 2565082 = NA (NA)intergenic (+68/‑109) LY180_12185/LY180_12190 D‑serine dehydratase/hypothetical protein
* ? CP006584 = 143231810 (0.160)53 (0.870) 49/584 0.3 84.8% coding (195/1614 nt) LY180_06775 peptide ABC transporter substrate‑binding protein
?CP006584 1435520 = 9 (0.150)coding (801/951 nt) LY180_06790 universal stress protein E
* ? CP006584 1643563 =50 (0.820)86 (1.450) 68/566 0.0 78.1% intergenic (‑223/‑80) LY180_07760/LY180_07765 sulfatase/transposase
?CP006584 = 4565632 0 (0.000)intergenic (‑372/+82) LY180_21775/LY180_21780 hypothetical protein/lysine 2,3‑aminomutase
* ? CP006584 1749406 =NA (NA)75 (1.220) 74/586 0.0 92.6% coding (13/864 nt) LY180_08300 transcriptional regulator
?CP006584 = 3920817 6 (0.100)coding (415/984 nt) LY180_18670 beta1,3‑glucosyltransferase
* ? CP006584 = 1750173NA (NA)70 (1.140) 63/586 0.1 100% coding (380/384 nt) LY180_08310 gdgsA
?CP006584 3920810 = 0 (0.000)coding (422/984 nt) LY180_18670 beta1,3‑glucosyltransferase
* ? CP006584 2030792 =NA (NA)46 (0.760) 43/578 0.5 100% intergenic (+74/+105) znuB/LY180_09680 zinc transporter/hypothetical protein
?CP006584 2414712 = 0 (0.000)coding (1018/1644 nt) LY180_11545 multidrug ABC transporter ATP‑binding protein
* ? CP006584 = 225383537 (0.600)14 (0.240) 14/556 3.0 26.9% coding (52/414 nt) LY180_10760 hypothetical protein
?CP006584 = 2255646 41 (0.710)coding (842/1284 nt) LY180_10775 tail protein
* ? CP006584 2385043 =2 (0.030)52 (0.850) 46/584 0.4 96.3% coding (718/1026 nt) LY180_11390 microcin C ABC transporter permease
?CP006584 2565085 = NA (NA)intergenic (+71/‑106) LY180_12185/LY180_12190 D‑serine dehydratase/hypothetical protein
* ? CP006584 = 23850523 (0.050)36 (0.590) 34/586 0.9 92.3% coding (727/1026 nt) LY180_11390 microcin C ABC transporter permease
?CP006584 2565084 = NA (NA)intergenic (+70/‑107) LY180_12185/LY180_12190 D‑serine dehydratase/hypothetical protein
* ? CP006584 = 2891935NA (NA)74 (1.210) 68/584 0.1 98.7% intergenic (+12/+249) LY180_13750/LY180_13755 hypothetical protein/transcriptional regulator
?CP006584 = 3268631 1 (0.020)coding (88/1119 nt) LY180_15515 hydrogenase 2 small subunit
* ? CP006584 2896407 =NA (NA)63 (1.040) 58/578 0.1 100% intergenic (+63/+105) LY180_13765/LY180_13770 6‑phospho‑beta‑glucosidase/transposase
?CP006584 3262157 = 0 (0.000)intergenic (+107/+16) LY180_15475/LY180_15480 GSH‑dependent disulfide bond oxidoreductase/hydrogenase 2 accessory protein HypG
* ? CP006584 = 31725930 (0.000)70 (1.160) 65/578 0.1 100% coding (28/273 nt) LY180_15050 hypothetical protein
?CP006584 4311141 = NA (NA)intergenic (‑130/+105) LY180_20655/LY180_20660 hypothetical protein/hypothetical protein
* ? CP006584 = 32621580 (0.000)69 (1.170) 65/562 0.1 100% intergenic (+108/+15) LY180_15475/LY180_15480 GSH‑dependent disulfide bond oxidoreductase/hydrogenase 2 accessory protein HypG
?CP006584 = 4312417 NA (NA)intergenic (‑191/+57) LY180_20660/LY180_20665 hypothetical protein/transcriptional regulator
* ? CP006584 3268622 =1 (0.020)78 (1.270) 68/586 0.1 98.7% coding (97/1119 nt) LY180_15515 hydrogenase 2 small subunit
?CP006584 3344744 = NA (NA)coding (163/330 nt) LY180_15880 hypothetical protein
* ? CP006584 3305035 =3 (0.050)46 (0.750) 40/584 0.6 93.9% intergenic (‑341/‑33) ribB/LY180_15715 3,4‑dihydroxy‑2‑butanone 4‑phosphate synthase/hypothetical protein
?CP006584 = 3672182 NA (NA)coding (475/477 nt) LY180_17575 membrane protein
* ? CP006584 = 33050454 (0.070)90 (1.470) 76/584 0.0 95.8% intergenic (‑351/‑23) ribB/LY180_15715 3,4‑dihydroxy‑2‑butanone 4‑phosphate synthase/hypothetical protein
?CP006584 3344745 = NA (NA)coding (164/330 nt) LY180_15880 hypothetical protein
* ? CP006584 3379202 =1 (0.020)76 (1.240) 66/586 0.1 98.7% intergenic (+70/‑172) LY180_16040/LY180_16045 membrane protein/membrane protein
?CP006584 = 3672183 NA (NA)coding (474/477 nt) LY180_17575 membrane protein
* ? CP006584 = 33792104 (0.070)53 (0.860) 48/586 0.3 93.0% intergenic (+78/‑164) LY180_16040/LY180_16045 membrane protein/membrane protein
?CP006584 = 3672183 NA (NA)coding (474/477 nt) LY180_17575 membrane protein
* ? CP006584 3513966 =18 (0.290)16 (0.570)
+158 bp
8/270 2.0 65.2% intergenic (‑142/+197) tldD/LY180_16725 protease TldD/membrane protein
?CP006584 = 3513990 19 (0.310)intergenic (‑166/+173) tldD/LY180_16725 protease TldD/membrane protein
* ? CP006584 4186759 =NA (NA)79 (1.290) 71/584 0.0 94.1% coding (292/294 nt) LY180_19995 hypothetical protein
?CP006584 4334567 = 5 (0.080)coding (30/1734 nt) LY180_20755 membrane protein
* ? CP006584 = 4188086NA (NA)85 (1.390) 73/586 0.0 97.7% coding (17/1167 nt) LY180_20005 hypothetical protein
?CP006584 = 4334576 2 (0.030)coding (21/1734 nt) LY180_20755 membrane protein
* ? CP006584 4496128 =NA (NA)58 (1.030) 56/538 0.1 100% intergenic (‑2037/‑83) LY180_21445/LY180_21450 hypothetical protein/hypothetical protein
?CP006584 4565632 = 0 (0.000)intergenic (‑372/+82) LY180_21775/LY180_21780 hypothetical protein/lysine 2,3‑aminomutase
* ? CP006584 4496731 =NA (NA)289 (4.720) 214/586 0.0 NA coding (521/1209 nt) LY180_21450 hypothetical protein
?CP006584 = 4497432 NA (NA)intergenic (+13/+464) LY180_21450/LY180_21455 hypothetical protein/D‑allose kinase
* ? CP006584 4739807 =60 (0.980)20 (0.340) 20/568 2.1 23.6% intergenic (+48/‑432) LY180_22625/fimA tyrosine recombinase/type‑1 fimbrial protein subunit A
?CP006584 4740121 = 71 (1.200)intergenic (+362/‑118) LY180_22625/fimA tyrosine recombinase/type‑1 fimbrial protein subunit A