breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 10,637 | C→A | G18G (GGC→GGA) | mogA → | molybdenum cofactor biosynthesis protein MogA |
RA | 194,161 | A→G | M42V (ATG→GTG) | LY180_00835 → | 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase |
RA | 230,529 | T→C | D275G (GAC→GGC) | LY180_01015 ← | LysR family transcripitonal regulator |
RA | 459,705 | Δ1 bp | coding (137/276 nt) | LY180_02175 ← | transcriptional regulator |
RA | 793,877 | T→C | C358R (TGT→CGT) | LY180_03720 → | rhsA |
RA | 793,936 | T→C | S377S (AGT→AGC) | LY180_03720 → | rhsA |
RA | 793,954 | A→G | E383E (GAA→GAG) | LY180_03720 → | rhsA |
RA | 793,985 | A→G | N394D (AAC→GAC) | LY180_03720 → | rhsA |
RA | 814,037 | G→C | T139S (ACC→AGC) | gltA ← | type II citrate synthase |
MC JC | 1,011,041 | Δ300 bp | intergenic (+21/+62) | LY180_04735 → / ← pflA | hypothetical protein/pyruvate formate lyase‑activating protein |
MC JC | 1,088,034 | Δ103 bp | intergenic (+278/+242) | helD → / ← LY180_05060 | DNA helicase IV/hypothetical protein |
RA | 1,168,401 | C→A | S606* (TCG→TAG) | LY180_05445 → | glucosyltransferase |
JC | 1,213,485 | Δ7 bp | intergenic (+184/‑105) | LY180_05705 → / → LY180_05710 | DNAse/PTS glucose transporter subunit IIBC |
RA | 1,324,434 | A→C | K833T (AAA→ACA) | narZ → | nitrate reductase A subunit alpha |
RA | 1,335,968 | A→G | intergenic (+281/+21) | LY180_06295 → / ← LY180_06300 | thymidine kinase/bifunctional acetaldehyde‑CoA/alcohol dehydrogenase |
MC JC | 1,335,989 | 2720 bp→18 bp | [LY180_06300]–[LY180_06310] | [LY180_06300], LY180_06305, [LY180_06310] | |
MC JC | 1,446,672 | Δ2,957 bp | [zntB]–[LY180_06850] | [zntB], LY180_06845, [LY180_06850] | |
RA | 1,459,895 | C→T | T49T (ACC→ACT) | LY180_06935 → | hypothetical protein |
RA | 1,464,821 | A→G | intergenic (+486/‑118) | LY180_06955 → / → LY180_06960 | hypothetical protein/regulatory protein |
RA | 1,505,743 | 2 bp→GT | intergenic (‑91/+19) | LY180_07195 ← / ← LY180_07200 | heat‑inducible protein/hypothetical protein |
RA | 1,505,880 | T→C | L35L (CTA→CTG) | LY180_07200 ← | hypothetical protein |
RA | 1,505,925 | A→G | I20I (ATT→ATC) | LY180_07200 ← | hypothetical protein |
RA | 1,505,928 | T→C | V19V (GTA→GTG) | LY180_07200 ← | hypothetical protein |
RA | 1,505,955 | T→C | L10L (CTA→CTG) | LY180_07200 ← | hypothetical protein |
RA | 1,563,485 | A→G | T256A (ACC→GCC) | LY180_07445 → | transposase IS609 |
RA | 1,563,642 | G→A | R308H (CGC→CAC) | LY180_07445 → | transposase IS609 |
RA | 1,563,706 | C→T | Y329Y (TAC→TAT) | LY180_07445 → | transposase IS609 |
RA | 1,563,726 | G→A | C336Y (TGC→TAC) | LY180_07445 → | transposase IS609 |
RA | 1,563,748 | A→G | E343E (GAA→GAG) | LY180_07445 → | transposase IS609 |
RA | 1,563,751 | T→C | N344N (AAT→AAC) | LY180_07445 → | transposase IS609 |
RA | 1,588,241 | A→C | E403D (GAA→GAC) | LY180_07560 → | type IV secretion protein Rhs |
RA | 1,645,397 | C→T | intergenic (+1191/+69) | LY180_07765 → / ← LY180_07770 | transposase/sulfatase |
RA | 1,645,421 | C→G | intergenic (+1215/+45) | LY180_07765 → / ← LY180_07770 | transposase/sulfatase |
MC JC | 1,749,406 | Δ777 bp | [LY180_08300]–[LY180_08310] | [LY180_08300], LY180_08305, [LY180_08310] | |
RA | 1,964,559 | G→A | N226N (AAC→AAT) | LY180_09345 ← | transposase IS609 |
RA | 1,964,699 | C→T | V180I (GTC→ATC) | LY180_09345 ← | transposase IS609 |
RA | 1,964,727 | G→T | S170S (TCC→TCA) | LY180_09345 ← | transposase IS609 |
RA | 1,964,784 | A→C | T151T (ACT→ACG) | LY180_09345 ← | transposase IS609 |
RA | 2,251,239 | G→A | P28P (CCC→CCT) | LY180_10740 ← | hypothetical protein |
RA | 2,251,586 | T→C | E104E (GAA→GAG) | LY180_10745 ← | major tail tube protein |
RA | 2,251,616 | A→G | R94R (CGT→CGC) | LY180_10745 ← | major tail tube protein |
RA | 2,265,347 | G→A | intergenic (‑100/‑74) | LY180_10855 ← / → LY180_10860 | precorrin‑8W decarboxylase/terminase |
RA | 2,265,396 | A→G | intergenic (‑149/‑25) | LY180_10855 ← / → LY180_10860 | precorrin‑8W decarboxylase/terminase |
RA | 2,265,399 | G→T | intergenic (‑152/‑22) | LY180_10855 ← / → LY180_10860 | precorrin‑8W decarboxylase/terminase |
RA | 2,265,885 | C→A | A155A (GCC→GCA) | LY180_10860 → | terminase |
RA | 2,312,761 | T→A | intergenic (‑71/+448) | LY180_11070 ← / ← LY180_11075 | hypothetical protein/hypothetical protein |
RA | 2,312,767 | 3 bp→GAC | intergenic (‑77/+440) | LY180_11070 ← / ← LY180_11075 | hypothetical protein/hypothetical protein |
RA | 2,312,771 | G→A | intergenic (‑81/+438) | LY180_11070 ← / ← LY180_11075 | hypothetical protein/hypothetical protein |
RA | 2,312,773 | C→G | intergenic (‑83/+436) | LY180_11070 ← / ← LY180_11075 | hypothetical protein/hypothetical protein |
RA | 2,483,647 | G→A | P236S (CCG→TCG) | LY180_11800 ← | NADH:ubiquinone oxidoreductase subunit M |
MC JC | 2,505,263 | Δ394 bp | intergenic (‑620/‑500) | LY180_11895 ← / → LY180_11905 | hypothetical protein/phosphate acetyltransferase |
RA | 2,505,816 | A→G | intergenic (‑1173/‑340) | LY180_11895 ← / → LY180_11905 | hypothetical protein/phosphate acetyltransferase |
RA | 2,506,026 | T→C | intergenic (‑1383/‑130) | LY180_11895 ← / → LY180_11905 | hypothetical protein/phosphate acetyltransferase |
MC JC | 2,606,939 | Δ122 bp | LY180_12380 | LY180_12380 | |
RA | 3,010,362 | G→T | E48* (GAA→TAA) | LY180_14330 → | type VI secretion protein ImpK |
RA | 3,278,551 | G→A | W246* (TGG→TGA) | LY180_15575 → | aldehyde oxidoreductase |
MC JC | 3,431,620 | Δ68 bp | coding (288‑355/879 nt) | LY180_16320 → | protease |
RA | 3,505,943 | C→T | G41D (GGT→GAT) | LY180_16680 ← | malate dehydrogenase |
RA | 3,545,689 | T→C | noncoding (2811/2931 nt) | LY180_16870 ← | 23S ribosomal RNA |
RA | 3,545,692 | C→T | noncoding (2808/2931 nt) | LY180_16870 ← | 23S ribosomal RNA |
RA | 3,545,695 | G→A | noncoding (2805/2931 nt) | LY180_16870 ← | 23S ribosomal RNA |
RA | 3,545,697 | A→G | noncoding (2803/2931 nt) | LY180_16870 ← | 23S ribosomal RNA |
JC | 3,812,239 | (AGA)2→4 | coding (434/1020 nt) | LY180_18170 ← | peptide ABC transporter permease |
RA | 3,943,262 | G→A | G302D (GGC→GAC) | LY180_18795 → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 4,023,563 | T→C | L4P (CTC→CCC) | rnpA → | ribonuclease P |
RA | 4,178,979 | T→C | noncoding (1009/1541 nt) | LY180_19945 → | 16S ribosomal RNA |
RA | 4,178,988 | Δ1 bp | noncoding (1018/1541 nt) | LY180_19945 → | 16S ribosomal RNA |
RA | 4,178,991 | A→T | noncoding (1021/1541 nt) | LY180_19945 → | 16S ribosomal RNA |
RA | 4,178,993 | +G | noncoding (1023/1541 nt) | LY180_19945 → | 16S ribosomal RNA |
RA | 4,179,007 | C→T | noncoding (1037/1541 nt) | LY180_19945 → | 16S ribosomal RNA |
RA | 4,305,124 | G→A | G42G (GGG→GGA) | LY180_20645 → | rhsA |
RA | 4,305,151 | G→C | P51P (CCG→CCC) | LY180_20645 → | rhsA |
RA | 4,305,201 | C→G | A68G (GCC→GGC) | LY180_20645 → | rhsA |
MC JC | 4,394,727 | Δ4,605 bp | LY180_21020–LY180_21030 | LY180_21020, adhP, LY180_21030 | |
RA | 4,469,025 | +A | coding (748/1350 nt) | LY180_21345 → | permase |
MC JC | 4,815,129 | Δ342 bp | coding (402‑743/1224 nt) | LY180_23005 → | phosphopentomutase |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | CP006584 | 1505958 | 1510585 | 4628 | 17 [15] | [16] 17 | [LY180_07200]–[LY180_07220] | [LY180_07200],LY180_07205,LY180_07210,LY180_07215,[LY180_07220] |
* | * | ÷ | CP006584 | 1644420–1644851 | 1645255–1644896 | 46–836 | 17 [16] | [16] 17 | LY180_07765/LY180_07770 | transposase/sulfatase |
* | * | ÷ | CP006584 | 2274637 | 2274808 | 172 | 17 [16] | [16] 17 | [LY180_10895] | [LY180_10895] |
* | * | ÷ | CP006584 | 3028823 | 3032159 | 3337 | 17 [16] | [16] 17 | [LY180_14390]–[LY180_14415] | [LY180_14390],LY180_14395,LY180_14400,LY180_14405,LY180_14410,[LY180_14415] |
* | * | ÷ | CP006584 | 3345698–3346066 | 3377332–3376397 | 30332–31635 | 17 [16] | [16] 17 | ebgC–LY180_16025 | ebgC,LY180_15890,LY180_15895,LY180_15900,fadH,LY180_15910,LY180_15915,LY180_15920,LY180_15925,LY180_15930,LY180_15935,LY180_15940,LY180_15945,LY180_15950,LY180_15955,LY180_15960,LY180_15965,LY180_15970,LY180_15975,LY180_15980,LY180_15985,LY180_15990,LY180_15995,LY180_16000,LY180_16005,LY180_16010,LY180_16015,LY180_16020,LY180_16025 |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP006584 | 5598 = | NA (NA) | 69 (1.140) | 55/564 | 0.2 | 98.6% | intergenic (+69/‑191) | LY180_00025/LY180_00030 | hypothetical protein/hypothetical protein |
? | CP006584 | 3172592 = | 1 (0.020) | coding (29/273 nt) | LY180_15050 | hypothetical protein | |||||
* | ? | CP006584 | = 225257 | NA (NA) | 65 (1.030) | 60/586 | 0.1 | NA | noncoding (1014/1541 nt) | LY180_00980 | 16S ribosomal RNA |
? | CP006584 | 225269 = | NA (NA) | noncoding (1026/1541 nt) | LY180_00980 | 16S ribosomal RNA | |||||
* | ? | CP006584 | = 269130 | 63 (1.000) | 53 (0.890) +TCATGTCATTAGTGCGTT |
47/552 | 0.3 | 64.2% | coding (377/414 nt) | LY180_01180 | hypothetical protein |
? | CP006584 | 1594499 = | 0 (0.000) | coding (168/909 nt) | LY180_07575 | hypothetical protein | |||||
* | ? | CP006584 | 294480 = | 32 (0.510) | 16 (0.290) | 14/520 | 3.0 | 33.6% | coding (26/996 nt) | LY180_01335 | hypothetical protein |
? | CP006584 | 297564 = | 35 (0.630) | coding (496/1515 nt) | LY180_01350 | tail protein | |||||
* | ? | CP006584 | = 294513 | 32 (0.510) | 22 (0.390) | 21/520 | 1.9 | 41.0% | coding (59/996 nt) | LY180_01335 | hypothetical protein |
? | CP006584 | = 297529 | 35 (0.630) | coding (531/1515 nt) | LY180_01350 | tail protein | |||||
* | ? | CP006584 | 346068 = | NA (NA) | 51 (0.810) | 48/588 | 0.4 | 96.2% | intergenic (‑51/‑107) | LY180_01675/LY180_01680 | CadC family transcriptional regulator/hypothetical protein |
? | CP006584 | = 363812 | 2 (0.030) | intergenic (‑39/‑249) | LY180_01770/LY180_01775 | outer membrane phosphoporin protein E/gamma‑glutamyl kinase | |||||
* | ? | CP006584 | = 347397 | 86 (1.360) | 61 (0.970) | 56/586 | 0.2 | 57.6% | intergenic (+14/‑444) | LY180_01680/LY180_01685 | hypothetical protein/flagellin |
? | CP006584 | 363805 = | 4 (0.060) | intergenic (‑32/‑256) | LY180_01770/LY180_01775 | outer membrane phosphoporin protein E/gamma‑glutamyl kinase | |||||
* | ? | CP006584 | 794676 = | NA (NA) | 35 (0.560) | 32/578 | 1.1 | NA | coding (1871/4200 nt) | LY180_03720 | rhsA |
? | CP006584 | = 794771 | NA (NA) | coding (1966/4200 nt) | LY180_03720 | rhsA | |||||
* | ? | CP006584 | 840689 = | 37 (0.590) | 47 (0.830) | 44/526 | 0.3 | 55.9% | intergenic (+68/‑82) | LY180_03930/LY180_03935 | tRNA‑Lys/tRNA‑Val |
? | CP006584 | = 841381 | 41 (0.730) | intergenic (+125/‑280) | LY180_03950/LY180_03955 | tRNA‑Lys/quinolinate synthetase | |||||
* | ? | CP006584 | 917080 = | NA (NA) | 56 (0.900) | 54/582 | 0.2 | 100% | intergenic (+88/‑191) | LY180_04320/LY180_04325 | heme ABC transporter ATP‑binding protein/hypothetical protein |
? | CP006584 | = 2414713 | 0 (0.000) | coding (1017/1644 nt) | LY180_11545 | multidrug ABC transporter ATP‑binding protein | |||||
* | ? | CP006584 | 1643563 = | 58 (0.920) | 96 (1.570) | 72/568 | 0.0 | 77.1% | intergenic (‑223/‑80) | LY180_07760/LY180_07765 | sulfatase/transposase |
? | CP006584 | = 4565632 | 1 (0.020) | intergenic (‑372/+82) | LY180_21775/LY180_21780 | hypothetical protein/lysine 2,3‑aminomutase | |||||
* | ? | CP006584 | 1684949 = | NA (NA) | 298 (4.720) | 217/588 | 0.0 | NA | intergenic (+452/+13) | LY180_07930/LY180_07935 | hypothetical protein/hypothetical protein |
? | CP006584 | = 1685650 | NA (NA) | coding (521/1209 nt) | LY180_07935 | hypothetical protein | |||||
* | ? | CP006584 | 1749406 = | NA (NA) | 66 (1.040) | 62/588 | 0.1 | 98.5% | coding (13/864 nt) | LY180_08300 | transcriptional regulator |
? | CP006584 | = 3920817 | 1 (0.020) | coding (415/984 nt) | LY180_18670 | beta1,3‑glucosyltransferase | |||||
* | ? | CP006584 | = 1750173 | NA (NA) | 79 (1.250) | 71/588 | 0.0 | 98.8% | coding (380/384 nt) | LY180_08310 | gdgsA |
? | CP006584 | 3920810 = | 1 (0.020) | coding (422/984 nt) | LY180_18670 | beta1,3‑glucosyltransferase | |||||
* | ? | CP006584 | 1840765 = | 6 (0.090) | 59 (0.930) | 54/588 | 0.2 | 90.8% | intergenic (+248/‑63) | LY180_08735/LY180_08740 | pyruvate kinase/murein lipoprotein |
? | CP006584 | = 1840785 | NA (NA) | intergenic (+268/‑43) | LY180_08735/LY180_08740 | pyruvate kinase/murein lipoprotein | |||||
* | ? | CP006584 | 2030792 = | NA (NA) | 43 (0.690) | 39/580 | 0.7 | 100% | intergenic (+74/+105) | znuB/LY180_09680 | zinc transporter/hypothetical protein |
? | CP006584 | 2414712 = | 0 (0.000) | coding (1018/1644 nt) | LY180_11545 | multidrug ABC transporter ATP‑binding protein | |||||
* | ? | CP006584 | 2253821 = | 21 (0.330) | 28 (0.470) | 28/558 | 1.3 | 60.3% | coding (38/414 nt) | LY180_10760 | hypothetical protein |
? | CP006584 | 2255662 = | 17 (0.280) | coding (826/1284 nt) | LY180_10775 | tail protein | |||||
* | ? | CP006584 | = 2253835 | 18 (0.280) | 32 (0.530) | 31/558 | 1.1 | 65.2% | coding (52/414 nt) | LY180_10760 | hypothetical protein |
? | CP006584 | = 2255646 | 17 (0.280) | coding (842/1284 nt) | LY180_10775 | tail protein | |||||
* | ? | CP006584 | 2385043 = | 1 (0.020) | 74 (1.180) | 62/586 | 0.1 | 98.7% | coding (718/1026 nt) | LY180_11390 | microcin C ABC transporter permease |
? | CP006584 | 2565085 = | NA (NA) | intergenic (+71/‑106) | LY180_12185/LY180_12190 | D‑serine dehydratase/hypothetical protein | |||||
* | ? | CP006584 | = 2385052 | 1 (0.020) | 39 (0.620) | 36/588 | 0.9 | 97.5% | coding (727/1026 nt) | LY180_11390 | microcin C ABC transporter permease |
? | CP006584 | 2565084 = | NA (NA) | intergenic (+70/‑107) | LY180_12185/LY180_12190 | D‑serine dehydratase/hypothetical protein | |||||
* | ? | CP006584 | = 2891935 | NA (NA) | 68 (1.080) | 57/586 | 0.2 | 97.2% | intergenic (+12/+249) | LY180_13750/LY180_13755 | hypothetical protein/transcriptional regulator |
? | CP006584 | = 3268631 | 2 (0.030) | coding (88/1119 nt) | LY180_15515 | hydrogenase 2 small subunit | |||||
* | ? | CP006584 | 2896407 = | NA (NA) | 56 (0.900) | 52/580 | 0.3 | 100% | intergenic (+63/+105) | LY180_13765/LY180_13770 | 6‑phospho‑beta‑glucosidase/transposase |
? | CP006584 | 3262157 = | 0 (0.000) | intergenic (+107/+16) | LY180_15475/LY180_15480 | GSH‑dependent disulfide bond oxidoreductase/hydrogenase 2 accessory protein HypG | |||||
* | ? | CP006584 | = 3172593 | 0 (0.000) | 71 (1.140) | 63/580 | 0.1 | 100% | coding (28/273 nt) | LY180_15050 | hypothetical protein |
? | CP006584 | 4311141 = | NA (NA) | intergenic (‑130/+105) | LY180_20655/LY180_20660 | hypothetical protein/hypothetical protein | |||||
* | ? | CP006584 | = 3262158 | 0 (0.000) | 50 (0.830) | 45/564 | 0.4 | 100% | intergenic (+108/+15) | LY180_15475/LY180_15480 | GSH‑dependent disulfide bond oxidoreductase/hydrogenase 2 accessory protein HypG |
? | CP006584 | = 4312417 | NA (NA) | intergenic (‑191/+57) | LY180_20660/LY180_20665 | hypothetical protein/transcriptional regulator | |||||
* | ? | CP006584 | 3268622 = | 7 (0.110) | 108 (1.710) | 74/588 | 0.0 | 93.9% | coding (97/1119 nt) | LY180_15515 | hydrogenase 2 small subunit |
? | CP006584 | 3344744 = | NA (NA) | coding (163/330 nt) | LY180_15880 | hypothetical protein | |||||
* | ? | CP006584 | 3305035 = | 3 (0.050) | 77 (1.220) | 67/586 | 0.1 | 96.3% | intergenic (‑341/‑33) | ribB/LY180_15715 | 3,4‑dihydroxy‑2‑butanone 4‑phosphate synthase/hypothetical protein |
? | CP006584 | = 3672182 | NA (NA) | coding (475/477 nt) | LY180_17575 | membrane protein | |||||
* | ? | CP006584 | = 3305045 | 2 (0.030) | 77 (1.220) | 65/586 | 0.1 | 97.5% | intergenic (‑351/‑23) | ribB/LY180_15715 | 3,4‑dihydroxy‑2‑butanone 4‑phosphate synthase/hypothetical protein |
? | CP006584 | 3344745 = | NA (NA) | coding (164/330 nt) | LY180_15880 | hypothetical protein | |||||
* | ? | CP006584 | 3379202 = | 1 (0.020) | 90 (1.420) | 76/588 | 0.0 | 98.9% | intergenic (+70/‑172) | LY180_16040/LY180_16045 | membrane protein/membrane protein |
? | CP006584 | = 3672183 | NA (NA) | coding (474/477 nt) | LY180_17575 | membrane protein | |||||
* | ? | CP006584 | = 3379210 | 4 (0.060) | 79 (1.250) | 69/588 | 0.1 | 95.2% | intergenic (+78/‑164) | LY180_16040/LY180_16045 | membrane protein/membrane protein |
? | CP006584 | = 3672183 | NA (NA) | coding (474/477 nt) | LY180_17575 | membrane protein | |||||
* | ? | CP006584 | = 3884875 | 79 (1.250) | 42 (0.670) | 37/586 | 0.8 | 33.8% | coding (905/1137 nt) | LY180_18515 | membrane protein |
? | CP006584 | 3885089 = | 86 (1.370) | coding (691/1137 nt) | LY180_18515 | membrane protein | |||||
* | ? | CP006584 | 4186759 = | NA (NA) | 88 (1.400) | 76/586 | 0.0 | 96.7% | coding (292/294 nt) | LY180_19995 | hypothetical protein |
? | CP006584 | 4334567 = | 3 (0.050) | coding (30/1734 nt) | LY180_20755 | membrane protein | |||||
* | ? | CP006584 | = 4188086 | NA (NA) | 62 (0.980) | 54/588 | 0.2 | 89.9% | coding (17/1167 nt) | LY180_20005 | hypothetical protein |
? | CP006584 | = 4334576 | 7 (0.110) | coding (21/1734 nt) | LY180_20755 | membrane protein | |||||
* | ? | CP006584 | 4496128 = | NA (NA) | 69 (1.190) | 63/540 | 0.1 | 93.8% | intergenic (‑2037/‑83) | LY180_21445/LY180_21450 | hypothetical protein/hypothetical protein |
? | CP006584 | 4565632 = | 5 (0.080) | intergenic (‑372/+82) | LY180_21775/LY180_21780 | hypothetical protein/lysine 2,3‑aminomutase |