BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.4%	3934.2 / NA	1245	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (5/3); new base T (671/564); total (677/568)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.2%	3767.6 / NA	1245	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (4/19); new base T (673/549); total (677/568)
Fisher's exact test for biased strand distribution p-value = 4.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1298 total aligned reads displayed.

TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420035‑420281

||

   attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                          <  2:3375264/128‑4 (MQ=255)

   attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                          >  1:769551/1‑125 (MQ=255)

    ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                         <  1:3368233/128‑5 (MQ=255)

     gcattcatACATTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAAAAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                        <  2:3119789/127‑6 (MQ=255)

      cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                       <  1:1273669/128‑1 (MQ=255)

      cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                       <  2:878339/128‑1 (MQ=255)

      cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                       <  1:2540259/128‑1 (MQ=255)

       attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                      >  2:1225233/1‑128 (MQ=255)

        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                     <  2:1122512/128‑1 (MQ=255)

         tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                    >  1:2509584/1‑128 (MQ=255)

           atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAAATGa                                                                                                                  >  1:2289515/1‑128 (MQ=255)

            tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                 <  2:2923970/128‑1 (MQ=255)

             aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                >  2:2378960/1‑128 (MQ=255)

             aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAAAAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                <  2:2556142/128‑1 (MQ=255)

                 ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                            <  2:2465575/128‑1 (MQ=255)

                 ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATCAattatt                                                                                                            >  1:1005681/1‑128 (MQ=255)

                  tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                           >  2:2151706/1‑128 (MQ=255)

                  tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                           <  2:2611652/128‑1 (MQ=255)

                     tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTATTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                        >  2:3826897/1‑128 (MQ=255)

                         aTAAATCTAAGTTTCCAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                    >  1:1677213/1‑128 (MQ=255)

                          tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                   >  2:1305772/1‑128 (MQ=255)

                            aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATTGt                                                                                                 >  1:933309/1‑128 (MQ=255)

                             aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                <  1:4024003/128‑1 (MQ=255)

                                  aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                           <  1:1169115/128‑1 (MQ=255)

                                  aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                           <  1:1114367/128‑1 (MQ=255)

                                  aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATAGTTAAatt                                                                                           >  1:1591660/1‑128 (MQ=255)

                                   gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                          <  2:1580428/128‑1 (MQ=255)

                                    tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                         <  2:640376/128‑1 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTTATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      >  2:3169175/1‑128 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                               >  1:2722868/1‑103 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      >  2:1809837/1‑128 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      <  1:3257992/128‑1 (MQ=255)

                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATATTTAATTTTAATAACTGAATTATTATACAAATTGTTAAATTAtatt                                                                                      >  1:882235/1‑128 (MQ=255)

                                       cTAAAATTATTTTAACATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      >  1:1051076/1‑128 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                     <  1:2367777/128‑1 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                     <  1:709475/128‑1 (MQ=255)

                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                     >  1:3425133/1‑128 (MQ=255)

                                         aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                    >  1:1220284/1‑128 (MQ=255)

                                          aaaTTTTTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                   <  2:2046848/128‑1 (MQ=255)

                                              tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                               >  1:2991061/1‑128 (MQ=255)

                                               attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                              >  1:1737739/1‑128 (MQ=255)

                                               attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                              >  1:3332828/1‑128 (MQ=255)

                                                 tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                            <  2:1735117/128‑1 (MQ=255)

                                                 tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                            >  1:3912045/1‑128 (MQ=255)

                                                  ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                           <  1:1888024/128‑1 (MQ=255)

                                                   tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                              <  1:2195876/124‑1 (MQ=255)

                                                   tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                          <  1:2914934/128‑1 (MQ=255)

                                                    aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                         <  2:1051076/128‑1 (MQ=255)

                                                      cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                       >  2:1394141/1‑128 (MQ=255)

                                                        tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTa                                                                     >  2:3519937/1‑128 (MQ=255)

                                                           aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                  >  1:3581720/1‑128 (MQ=255)

                                                             aataataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                <  1:3478389/125‑1 (MQ=255)

                                                             aaaaaTAATATTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                <  1:1491254/128‑1 (MQ=255)

                                                             aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                                <  1:1826099/128‑1 (MQ=255)

                                                              aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAt                                                               >  1:3849897/1‑128 (MQ=255)

                                                               aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                              <  1:1432398/128‑1 (MQ=255)

                                                               aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTATTAATAAAtt                                                              >  1:3528748/1‑128 (MQ=255)

                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                             <  1:616899/128‑1 (MQ=255)

                                                                 ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                    <  2:2694190/88‑1 (MQ=255)

                                                                     taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                        >  2:2308653/1‑128 (MQ=255)

                                                                      aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                       >  2:573052/1‑128 (MQ=255)

                                                                      aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                       >  2:1882230/1‑128 (MQ=255)

                                                                      aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTATTCAAATTGTTAAATTAATTTCAAATCATTATCTATTAATCAATTTTATTCt                                                       >  1:2583502/1‑128 (MQ=255)

                                                                        ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAATTCATTATCTATTAATCAATTTTATGCTC‑‑g                                                   >  2:345985/1‑128 (MQ=255)

                                                                         tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑t                                                >  1:241469/1‑128 (MQ=255)

                                                                         tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTctt                                                    >  1:470167/1‑126 (MQ=255)

                                                                          ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑t‑‑tt                                               >  2:397000/1‑128 (MQ=255)

                                                                           tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑ttt                                              >  2:3694477/1‑128 (MQ=255)

                                                                            attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑tttt                                             >  2:3640093/1‑128 (MQ=255)

                                                                                 tttttatatTATAAAATTATAAGTACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATCTTTTAATCAATTTTATTCTC‑‑G‑‑ttttttttt                                        <  2:3578571/124‑1 (MQ=255)

                                                                                  tcatataatataAAAATAATATCACTTTTTTAAAAAATTTTTAATTTTAAAAAATTAATTATTTTTAAAATTGTTAAATTATTTTCAAAACATTATATATTAAACAATTTTATGCTC‑‑G‑‑tttttttttt                                       <  2:753659/126‑1 (MQ=255)

                                                                                   gATATATTAGAAAAATAATATCACTTTTTTAAAAAATTTTTAAATTTAATAACTGAATTTTTTTTCAAATTTTTAAATTATTTTCAAATCATTATCAAATAATCAATTTTATGCTC‑‑G‑‑ttttttttttt                                      <  1:968661/128‑1 (MQ=255)

                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑tttttttttttttt                                   >  1:2794419/1‑128 (MQ=255)

                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTATCAAATTGTTAAATTATTTTCAAATCATAATCTATTAAACAATATTATGCTC‑‑G‑‑tatttttttatttt                                   >  1:2024622/1‑128 (MQ=255)

                                                                                          tAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑ttttttttttttttttaa                               >  1:3957445/1‑126 (MQ=255)

                                                                                           aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑tttttttttttttttttta                              >  1:1959932/1‑126 (MQ=255)

                                                                                             aaaaCTACTATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTAATTTAAAATCATTATCTAATAATAAATTTTATTCTC‑‑G‑‑ttttttttttttttttttaca                            <  1:2250190/128‑5 (MQ=255)

                                                                                              aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑ttttttttttttttttttaaaa                           >  2:2754015/1‑123 (MQ=255)

                                                                                              aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTAaaaa                           >  1:839598/1‑128 (MQ=255)

                                                                                              aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTATATCATC‑‑G‑‑tttttttttttttttttttaaa                           >  1:2095911/1‑123 (MQ=255)

                                                                                              aaaCTAATAGCAATTTTTTAAAAAATTTTTAAATTTAATAAATGTATTATTTTTAAAATTGTTAAAATTTTTTAAAATAAATATAAATTAAACAATTTTATGCTC‑‑G‑‑ttttttttttttttttttacaa                           <  1:3199769/128‑6 (MQ=255)

                                                                                                  tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTTaaaaaaat                       >  2:3748169/1‑126 (MQ=255)

                                                                                                  tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCAATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTAAAAAAAata                       >  1:3768154/1‑128 (MQ=255)

                                                                                                  taatatcACTTTTTTATAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAata                       <  2:941839/122‑1 (MQ=255)

                                                                                                   actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑GT‑TTTTTTTTTTTTTTTTTAAAAAAata                       >  2:3315374/1‑128 (MQ=255)

                                                                                                    ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTATC‑‑G‑‑TTTTTTTTTTTTTTTTTTTaaaaaaaat                     >  1:2655486/1‑124 (MQ=255)

                                                                                                     tagtaattttttAAAATATTTTTATTTTAATTAACTATTTTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTAATGATC‑‑G‑‑‑TTTTTTTTTTTTTTTTACAAAAATATAat                   <  1:557289/122‑1 (MQ=255)

                                                                                                     taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑GT‑TTTTTTTTTTTTTTTTTAAAAAAataaa                     >  1:307940/1‑126 (MQ=255)

                                                                                                     taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑GT‑TTTTTTTTTTTTTTTTTAAAAAAAtata                     >  1:1323138/1‑128 (MQ=255)

                                                                                                       gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑GT‑TTTTTTTTTTTTTTTTTAAAAAAATATAat                   >  2:433121/1‑128 (MQ=255)

                                                                                                        cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑GT‑TTTTTTTTTTTTTTTTTAAAAAAATATAatt                  >  2:2238484/1‑128 (MQ=255)

                                                                                                         aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑GTTTTTTTTTTTTTTTTTTTACAAAAATATAatt                  >  2:3273173/1‑128 (MQ=255)

                                                                                                           ttttttAAAAAATTT‑TAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAATAAAatttat               >  1:1391098/1‑128 (MQ=255)

                                                                                                             ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAat                   <  1:3147754/121‑1 (MQ=255)

                                                                                                               ttAAAAAATTTTTTATTTTAATAAATTAATTATTTTTAAAATTTTTTAATTTTTTTAAAAAAATTTTATATTAATAAATTTTTTGCTC‑‑G‑‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt         <  1:3718024/128‑1 (MQ=255)

                                                                                                               taaaaaaaTATTAAATT‑TAATAAATTAATTTATTTTCAAATATTTAAATTATTTTATAATCTTTATCTTTTAATCAATTTTATGCTC‑‑GT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAt          <  2:3235455/126‑1 (MQ=255)

                                                                                                                 aaaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaa       >  1:3654422/1‑128 (MQ=255)

                                                                                                                    aaaTTTTTAATTTTAATAAATTTATTTTTTTTAAAATTTTTAAATTTTTTTCAAATAATTATCTATTAATCAATTTTATGCTC‑‑GT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa      <  2:3011835/128‑1 (MQ=255)

                                                                                                                     aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTC‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAATATAATTATTTtaaataaa      >  2:1489284/1‑128 (MQ=255)

                                                                                                                     aaTTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTTTTTTTAAATTATTATTTATTAATAAATTTTATTCTCTTT‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa      <  2:2824049/128‑1 (MQ=255)

||

TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTC‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420035‑420281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`