Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 97,235 C→A S207Y (TCT→TAT)  JCVSYN2_00390 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681697,2350CA99.9% 6559.4 / NA 2104S207Y (TCT→TAT) JCVSYN2_00390hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (1065/1035);  minor base T (1/2);  total (1066/1038)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2133 total aligned reads displayed.

TGAGTTTGTTAAAGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTCTAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTCCTAAAGGAATTTCG  >  CP016816/97110‑97359
                                                                                                                             |                                                                                                                            
 gAGTTTGTTAAAGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATaa                                                                                                                           >  2:719318/1‑128 (MQ=255)
   gTTTGTTAAAGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAAtt                                                                                                                         <  1:2697161/128‑1 (MQ=255)
     ttGTTAAAGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTa                                                                                                                       <  1:241161/128‑1 (MQ=255)
      tGTTAAAGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAg                                                                                                                      >  1:752778/1‑128 (MQ=255)
       gTTAAAGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGa                                                                                                                     >  1:1423432/1‑128 (MQ=255)
       gTTAAAGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGa                                                                                                                     >  1:1670612/1‑128 (MQ=255)
           aaGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCt                                                                                                                 <  2:3221484/128‑1 (MQ=255)
           aaGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCt                                                                                                                 <  2:112096/128‑1 (MQ=255)
            aGATTAATTATTTCACATTAGTAAAAACAATTATTTATTTTATATTATTTAATATGTTTAAATATCAATTTTTAGTTTATTAAAATACTATATAAATAATGTTTTTTATATTTATAATTTAGATGCTa                                                                                                                <  1:1503047/128‑1 (MQ=255)
            aGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTa                                                                                                                <  1:1227666/128‑1 (MQ=255)
              aTGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTAtt                                                                                                              >  1:1068019/1‑128 (MQ=255)
               tGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATTGTTCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTg                                                                                                             <  2:353456/128‑1 (MQ=255)
                gAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGt                                                                                                            >  2:2472487/1‑128 (MQ=255)
                 aaTTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGtt                                                                                                           >  2:3006625/1‑128 (MQ=255)
                 aaTTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGtt                                                                                                           <  1:3762527/128‑1 (MQ=255)
                 aaTTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGtt                                                                                                           >  1:892164/1‑128 (MQ=255)
                 aaTTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGATATTGtt                                                                                                           >  2:592507/1‑128 (MQ=255)
                  attattTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTta                                                                                                          >  2:2648839/1‑128 (MQ=255)
                  attattTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTta                                                                                                          <  1:2755506/128‑1 (MQ=255)
                      tttCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATAtt                                                                                                      >  2:2690886/1‑128 (MQ=255)
                       ttCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATAttt                                                                                                     <  1:398710/128‑1 (MQ=255)
                         cacaGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTc                                                                                                   >  1:307065/1‑128 (MQ=255)
                            agtagtAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATTCTATAGTTATATTTTCAAt                                                                                                >  1:3705538/1‑128 (MQ=255)
                            agtagtAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAAt                                                                                                <  1:829905/128‑1 (MQ=255)
                             gtagtaAAAACAATTCTTTATTTTATATTAGAGAAGATGGTTCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAAtt                                                                                               <  1:1000275/128‑1 (MQ=255)
                              tagtaAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATta                                                                                              <  2:1829128/128‑1 (MQ=255)
                              tagtaAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATta                                                                                              <  2:3151168/128‑1 (MQ=255)
                               agtaAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATtaa                                                                                             >  1:1913158/1‑128 (MQ=255)
                               agtaAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATtaa                                                                                             >  1:1995138/1‑128 (MQ=255)
                               agtaAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATtaa                                                                                             <  1:212188/128‑1 (MQ=255)
                                 taAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATTATATTGTTATATTTTCAATtaata                                                                                           >  1:2562997/1‑128 (MQ=255)
                                  aaaaaCAATTCTTTCTTTTATATTAGAGAAGATTGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATtaataa                                                                                          <  1:2308253/128‑1 (MQ=255)
                                  aaaaaCAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATtaataa                                                                                          >  1:422898/1‑128 (MQ=255)
                                        aaTTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGAc                                                                                    >  1:975189/1‑128 (MQ=255)
                                        aaTTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGAc                                                                                    <  2:104479/128‑1 (MQ=255)
                                          ttCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACtt                                                                                  <  1:1354716/128‑1 (MQ=255)
                                          ttCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACtt                                                                                  <  2:800381/128‑1 (MQ=255)
                                          ttCTTTATATGATATTAGAGAAGATGGTGAACTACAAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACtt                                                                                  <  1:607860/128‑1 (MQ=255)
                                           tCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACttt                                                                                 <  2:2933057/128‑1 (MQ=255)
                                              ttattttatATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACtttt                                                                                >  2:2080503/1‑126 (MQ=255)
                                               tattttatATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCaa                                                                             >  1:1823544/1‑128 (MQ=255)
                                                attttatATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCaat                                                                            >  2:278386/1‑128 (MQ=255)
                                                 ttttatATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATCGTTATATTTTCAATTAATAATAAGACTTTTCaatt                                                                           >  2:3383276/1‑128 (MQ=255)
                                                  tttatATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCaatta                                                                          >  2:3960816/1‑128 (MQ=255)
                                                    tataTTAGAGAAGATGGTGCACTACAAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCaattaat                                                                        <  2:2408136/128‑1 (MQ=255)
                                                         tAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGt                                                                   <  1:3445547/128‑1 (MQ=255)
                                                           gagaAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTTGGTta                                                                 >  2:3096358/1‑128 (MQ=255)
                                                           gagaAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTta                                                                 <  2:2595382/128‑1 (MQ=255)
                                                           gagaAGATGGAGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTta                                                                 <  1:1563948/128‑1 (MQ=255)
                                                             gaagaTGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACtttt                                                                                >  2:2248612/1‑111 (MQ=255)
                                                               agaTGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATAtt                                                             >  2:3818817/1‑128 (MQ=255)
                                                               agaTGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATAtt                                                             <  1:522688/128‑1 (MQ=255)
                                                                 aTGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCa                                                           <  1:3031040/128‑1 (MQ=255)
                                                                  tGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCaa                                                          >  1:3537316/1‑128 (MQ=255)
                                                                  tGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCaa                                                          <  2:2008162/128‑1 (MQ=255)
                                                                        aCTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATg                                                    <  1:681607/128‑1 (MQ=255)
                                                                        aCTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATg                                                    <  2:439419/128‑1 (MQ=255)
                                                                         cTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTggg                                                                    >  2:1762502/1‑111 (MQ=255)
                                                                         cTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTc                                                            >  2:1350200/1‑119 (MQ=255)
                                                                          tACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGaa                                                  >  1:3900348/1‑128 (MQ=255)
                                                                            ccAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATAATTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAAt                                                >  1:2483058/1‑128 (MQ=255)
                                                                             cAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAAtt                                               <  2:3597267/128‑1 (MQ=255)
                                                                              aaTTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTg                                              >  2:1486444/1‑128 (MQ=255)
                                                                               aTTTATAGTTTATTAAAATACTATAGGAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGa                                             <  2:3055318/128‑1 (MQ=255)
                                                                                 ttATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTCTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATg                                           >  2:253803/1‑128 (MQ=255)
                                                                                  tataTTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGt                                          <  2:3296583/128‑1 (MQ=255)
                                                                                  tataGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGt                                          <  1:2051947/128‑1 (MQ=255)
                                                                                    taGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGttt                                        >  1:1754603/1‑128 (MQ=255)
                                                                                     aGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGtttt                                       >  1:3367504/1‑128 (MQ=255)
                                                                                          attaAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGTTTATATTCAAAAAATGAAATTGATGTTTTAGAAc                                  >  1:3131896/1‑128 (MQ=255)
                                                                                          attaAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAAc                                  <  1:1622387/128‑1 (MQ=255)
                                                                                           ttaAAATACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGAAGTTTAATAACt                                 >  2:653568/1‑128 (MQ=255)
                                                                                              aaaTACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACtttt                              <  2:1679678/128‑1 (MQ=255)
                                                                                              aaaTACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACtttt                              <  2:3888144/128‑1 (MQ=255)
                                                                                              aaaTACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACtttt                              <  1:3587284/128‑1 (MQ=255)
                                                                                               aaTACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTa                             <  2:2134089/128‑1 (MQ=255)
                                                                                                aTACTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAg                            <  2:514244/128‑1 (MQ=255)
                                                                                                  aCTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGAt                          >  2:1921184/1‑128 (MQ=255)
                                                                                                  aCTATAGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGAt                          >  1:3283425/1‑128 (MQ=255)
                                                                                                   cTATATTAATATAGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATg                         <  2:4018968/128‑1 (MQ=255)
                                                                                                   cTATAGTAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATg                         <  2:1555477/128‑1 (MQ=255)
                                                                                                   cTATAGATATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATg                         <  1:2228956/128‑1 (MQ=255)
                                                                                                     ataGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCt                       >  1:2811651/1‑128 (MQ=255)
                                                                                                     ataGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCt                       >  1:3211661/1‑128 (MQ=255)
                                                                                                     ataGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCt                       >  2:3747934/1‑128 (MQ=255)
                                                                                                     ataGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCt                       >  2:1210694/1‑128 (MQ=255)
                                                                                                       aGAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTAt                     >  2:872936/1‑128 (MQ=255)
                                                                                                        gAAATAATGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTAtt                    >  2:16163/1‑128 (MQ=255)
                                                                                                          aataatGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTATATGCTATTGt                  >  2:368737/1‑128 (MQ=255)
                                                                                                           ataatGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGtt                 <  2:1398473/128‑1 (MQ=255)
                                                                                                            taatGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTc                <  2:93347/128‑1 (MQ=255)
                                                                                                            taatGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTc                >  1:3842562/1‑128 (MQ=255)
                                                                                                            taatGTGTTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTc                >  2:2200574/1‑128 (MQ=255)
                                                                                                            taatGTGTTTTATATTTATAATTTAGATGCTAGTGTTATATTTTCAATTAATATTAAGACTTTTCAATTATTTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTc                <  1:2620333/128‑1 (MQ=255)
                                                                                                                  gtTTTATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTCCTAAAg          <  1:1138854/128‑1 (MQ=255)
                                                                                                                  gtTTTATATTTATAATTTAGATGCTATTGTTATAGTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTCCTAAAg          <  2:2321753/128‑1 (MQ=255)
                                                                                                                   ttttATATTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTCCTAAAgg         >  1:2900872/1‑128 (MQ=255)
                                                                                                                    tttatatttataATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTCCTAAAGGa        >  1:298413/1‑128 (MQ=255)
                                                                                                                      tataTTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAATTGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTCCTAAAGGAAt      <  2:2863105/128‑1 (MQ=255)
                                                                                                                       atatTTATAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTCCTAAAGGAAtt     >  2:344838/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
TGAGTTTGTTAAAGATGAATTATTTCACAGTAGTAAAAACAATTCTTTATTTTATATTAGAGAAGATGGTGCACTACCAATTTATAGTTTATTAAAATACTATAGAAATAATGTGTTTTATATTTCTAATTTAGATGCTATTGTTATATTTTCAATTAATAATAAGACTTTTCAATTAATTGGGTTATATTCAAAAAATGAAATTGATGTTTTAGAACTTTTAGATGCTATTGTTCCTAAAGGAATTTCG  >  CP016816/97110‑97359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: