BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	32,707	A→T	I1067F (ATT→TTT)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	32,707	0	A	T	99.4%	5645.0 / NA	1749	I1067F (ATT→TTT)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (6/5); new base T (881/852); total (889/860)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1813 total aligned reads displayed.

TTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32582‑32831

ttACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAACAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCAtttttt                                                                                                                            >  2:2400407/1‑128 (MQ=255)

   cTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaaa                                                                                                                         <  2:2206183/128‑1 (MQ=255)

    tCAAGCTTTAACTCAGTTTTTTGGAGAAATTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAAt                                                                                                                        <  2:1269007/128‑1 (MQ=255)

    tCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAAt                                                                                                                        <  1:1574570/128‑1 (MQ=255)

      aaCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCATTCATTTTTTAAATCt                                                                                                                      >  1:3335033/1‑128 (MQ=255)

          tttAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAtt                                                                                                                  <  2:2274460/128‑1 (MQ=255)

            tAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                <  2:688417/128‑1 (MQ=255)

              aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              <  1:2038806/128‑1 (MQ=255)

                   gTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAg                                                                                                           <  2:1960449/126‑1 (MQ=255)

                   gTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAg                                                                                                           >  1:1960449/1‑126 (MQ=255)

                    tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              >  1:704768/1‑122 (MQ=255)

                    tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGa                                                                                                        >  2:1440173/1‑128 (MQ=255)

                    tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGa                                                                                                        >  1:1238981/1‑128 (MQ=255)

                       gttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtc                                                                                                     >  2:2960165/1‑128 (MQ=255)

                       gttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtc                                                                                                     >  2:3201902/1‑128 (MQ=255)

                             gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               <  2:2039486/128‑1 (MQ=255)

                              aaCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                              <  1:1883154/128‑1 (MQ=255)

                               aCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAagag                                                                                             <  1:1114207/128‑1 (MQ=255)

                                cTTTTAAAAACTATTTTTAAAGATAATGTGCAAACTACAGGTGAGTGAAAAGAAAATATCTTAAACTAAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGc                                                                                            <  2:1197178/128‑1 (MQ=255)

                                 ttttAAAAACTATTTTTAAAGATAAAGTGCAATCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                           <  1:3718314/128‑1 (MQ=255)

                                  tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                          <  2:1596874/128‑1 (MQ=255)

                                   ttAAAATCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                         <  1:1165685/128‑1 (MQ=255)

                                   ttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                         <  2:3919818/128‑1 (MQ=255)

                                     aaaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAt                                                                                       <  1:3849805/128‑1 (MQ=255)

                                     aaaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAt                                                                                       <  2:537019/128‑1 (MQ=255)

                                     aaaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTATAGGTTATTCTCCTTAATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAt                                                                                       <  1:2800705/128‑1 (MQ=255)

                                      aaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtt                                                                                      <  2:234199/128‑1 (MQ=255)

                                       aatagtttttaAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAttt                                                                                     >  1:2875449/7‑128 (MQ=255)

                                         aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                   >  1:181844/1‑128 (MQ=255)

                                          cTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCt                                                                                  >  2:3880149/1‑128 (MQ=255)

                                          cTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCt                                                                                  <  1:3623640/128‑1 (MQ=255)

                                           tATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtt                                                                                 >  1:2244330/1‑128 (MQ=255)

                                           tATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtt                                                                                 <  1:341861/128‑1 (MQ=255)

                                            aTTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCttt                                                                                >  2:3506123/1‑128 (MQ=255)

                                              ttttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAg                                                                                                           >  2:2665780/1‑99 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACATGTGAGTGAAAAGAAAAGATCTTAAACATTATTTTAGGTTATTCTACTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  1:3563562/128‑1 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  2:2773628/128‑1 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATATAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  2:2525349/1‑128 (MQ=255)

                                               tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGAACTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTACTTTTgg                                                                             >  1:2955274/1‑128 (MQ=255)

                                                   aaGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGa                                                                         >  1:2366672/1‑128 (MQ=255)

                                                    aGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGAc                                                                        >  2:1360101/1‑128 (MQ=255)

                                                     gATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTTACa                                                                       >  2:932185/1‑128 (MQ=255)

                                                     gATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACa                                                                       <  1:3073144/128‑1 (MQ=255)

                                                      aTAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                         <  1:1722067/109‑1 (MQ=255)

                                                         aaGTGCAAACTACAGGTGAGTGAAAAGTAAAGATCTTAAACTTAATTTTAGGGTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                   <  1:3785568/128‑1 (MQ=255)

                                                         aaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                   >  1:3260787/1‑128 (MQ=255)

                                                          agttaaaaCTACAGGTGAGTTAAAAGATAAGATCTTAAACTTATTTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  <  1:2115468/123‑1 (MQ=255)

                                                          aGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTACGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  <  2:2617410/128‑1 (MQ=255)

                                                            tGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTGAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCCTTTGGTTGACAAaaacaa                                                                >  1:3905158/1‑128 (MQ=255)

                                                            tGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCTAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaa                                                                >  2:862009/1‑128 (MQ=255)

                                                             gCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAatacaaa                                                               >  2:1778947/1‑128 (MQ=255)

                                                              cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGATGAGCTCAATTTTCTTTTGGTTGACAAaaacaaac                                                              >  1:3990771/1‑128 (MQ=255)

                                                              cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacacac                                                              >  2:381944/1‑128 (MQ=255)

                                                              cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaaac                                                              >  1:315066/1‑128 (MQ=255)

                                                              cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTATCTTATGGTTGACAAaaacaaac                                                              >  2:2154900/1‑128 (MQ=255)

                                                              cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCAGTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCACAATTTTCTTTAGTTTGACAAaaactaac                                                              >  1:833393/1‑128 (MQ=255)

                                                               aaaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACt                                                             <  1:1427141/128‑1 (MQ=255)

                                                                taCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                            <  2:1058148/127‑1 (MQ=255)

                                                                aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGATGAGCTCAATTATCTTTTGGTTGACAAAATCAAACta                                                            >  1:1001493/1‑128 (MQ=255)

                                                                aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  1:2179733/1‑111 (MQ=255)

                                                                    aCAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAAt                                                        >  1:2419718/1‑128 (MQ=255)

                                                                          gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCaaca                                                  >  1:2694211/1‑128 (MQ=255)

                                                                           agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                 <  1:979227/128‑1 (MQ=255)

                                                                           agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                 <  1:1348946/128‑1 (MQ=255)

                                                                            gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATTATTCCACaaa                                                >  2:1141445/1‑128 (MQ=255)

                                                                            gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaa                                                >  2:4013896/1‑128 (MQ=255)

                                                                             tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaaa                                               >  2:3431458/1‑128 (MQ=255)

                                                                                 aagaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGAt                                           >  1:1048268/1‑128 (MQ=255)

                                                                                    aatagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCaa                                        <  2:166226/125‑1 (MQ=255)

                                                                                      aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGCGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  2:599955/1‑128 (MQ=255)

                                                                                      aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACtata                                                          <  1:2484806/108‑1 (MQ=255)

                                                                                      aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  2:3594332/1‑128 (MQ=255)

                                                                                       agaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAATCTATAATTCCACAAAAATATCAATTa                                     >  1:2615901/1‑128 (MQ=255)

                                                                                        taTCTTAAACTTAAATTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  1:913286/127‑1 (MQ=255)

                                                                                        gaTCTTAATCTTAATTTTAGTTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAATATCTCAATTTTCTTTTGGTTGACAAAAACAAACTAAAATTCCACAAAAAGATCAATTAg                                    <  1:2292927/128‑1 (MQ=255)

                                                                                        gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  1:3212938/128‑1 (MQ=255)

                                                                                        gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  2:3257050/128‑1 (MQ=255)

                                                                                        gaTCTTAAACTTAATTTTAGGATATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAATATCAATTAg                                    <  1:569633/128‑1 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               >  2:1866562/1‑128 (MQ=255)

                                                                                             tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               >  2:336998/1‑128 (MQ=255)

                                                                                               aaCTTAATTTTAGTTTATTCTACTTATTTTTTTAAATCATATTTAACAACTTAAACTCTAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAt                             <  1:2553762/128‑1 (MQ=255)

                                                                                               aaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCTAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    >  1:780284/1‑121 (MQ=255)

                                                                                               aaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  2:780284/121‑1 (MQ=255)

                                                                                               aaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAt                             >  2:3708950/1‑128 (MQ=255)

                                                                                                  ttAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtttt                          >  2:3014658/1‑128 (MQ=255)

                                                                                                   tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCTACTAttttt                         >  1:349626/1‑128 (MQ=255)

                                                                                                    atttttAGGTTAATCTACTTCATTTTTTAAATCTTATTTAACAACTTAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTa                        <  1:617999/126‑1 (MQ=255)

                                                                                                     aTTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTaa                       >  2:1531061/1‑128 (MQ=255)

                                                                                                        ttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAcc                    <  2:2107051/128‑1 (MQ=255)

                                                                                                        agcccttATTCTCCATCAATTTATAAATCTTATATAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAcc                    <  1:1659036/123‑1 (MQ=255)

                                                                                                         tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAATCTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCt                   >  2:284794/1‑128 (MQ=255)

                                                                                                          aGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTa                  <  2:1727345/128‑1 (MQ=255)

                                                                                                            gTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  >  2:311594/1‑78 (MQ=255)

                                                                                                             ttATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAAt               <  1:494061/128‑1 (MQ=255)

                                                                                                                ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCGATTAGCAACTATTTTTAAACCTAAATCaa            >  2:3656345/1‑128 (MQ=255)

                                                                                                                ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa            >  1:3004534/1‑128 (MQ=255)

                                                                                                                  ctcCTTAATTTTTTAATTCTTTTTTAACAAGTGAATATCGAAGAGCTGAAATTTCTTTTGGTTGACAAAAACAATCTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAAt          <  2:475897/128‑1 (MQ=255)

                                                                                                                    ccTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATaa        >  1:43802/1‑128 (MQ=255)

                                                                                                                       tCATTTTTTAAATCTTATTTAACAAGTGAATCTAGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt     <  2:2438240/128‑1 (MQ=255)

                                                                                                                         aTTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTg   >  1:1517138/1‑128 (MQ=255)

TTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32582‑32831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`