Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,793 A→T intergenic (‑102/+69) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7930AT97.2% 5355.5 / 58.2 1818intergenic (‑102/+69)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (31/19);  new base T (874/891);  total (908/910)
Fisher's exact test for biased strand distribution p-value = 8.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1871 total aligned reads displayed.

TATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484667‑484913
                                                                                                                              |                                                                                                                         
tattTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAAATACACTCGATTATATAATTATATTTTATTTTTaat                                                                                                                          >  1:1426757/1‑127 (MQ=255)
      aaaaaTGGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                   <  2:1453685/128‑1 (MQ=255)
      aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                   >  2:2158431/1‑128 (MQ=255)
        aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                 >  1:1005389/1‑126 (MQ=255)
        aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                 >  2:3572257/1‑126 (MQ=255)
        aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaaga                                                                                                                 >  1:1519726/1‑126 (MQ=255)
         aaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCa                                                                                                                <  1:2089616/128‑1 (MQ=255)
          aTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCaa                                                                                                               >  2:2958048/1‑128 (MQ=255)
           tAGTAAAATCTCATAGATTTAATCCTTTTATAGTTGACAAAAAAATAATATAGAGCATAGAATAAGCTATTAAAAAATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                              <  1:2351896/128‑1 (MQ=255)
           tAGGCAAAACTCTTAGATTTAATACTATTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTTACAACTAAACTAAACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                              <  2:2495869/128‑1 (MQ=255)
            aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAATCTCTTAACAACTAAACTACAATCGATTATTTAATTATATTATTTTTTAATATATAAAGCAAtt                                                                                                             >  2:2615257/1‑128 (MQ=255)
             gTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTa                                                                                                            <  2:2795511/128‑1 (MQ=255)
             gTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTa                                                                                                            >  1:3483927/1‑128 (MQ=255)
              tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                           >  1:1772274/1‑128 (MQ=255)
                  aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                       >  1:1313011/1‑128 (MQ=255)
                      cATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaa                                                                                                   <  1:989531/128‑1 (MQ=255)
                       aTAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                  <  1:3857894/128‑1 (MQ=255)
                        tcgATTTAATCCTTTATTAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                 <  2:1800671/126‑1 (MQ=255)
                        tAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                  >  1:2003129/1‑128 (MQ=255)
                          gatataaaacTTTTATAGTTGACAAAAAAATAAGATAGAGCATATCAGAAGATCTTAACAAATAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                               <  1:3097495/119‑1 (MQ=255)
                            tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                             >  1:326750/1‑128 (MQ=255)
                            tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                             <  1:2840147/128‑1 (MQ=255)
                            tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACACGATTATTTAATTATATTTTTTATTAATATAAAAATAAATTAAAAACACAAAAACTc                                                                                             >  1:1637091/1‑128 (MQ=255)
                             ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                            <  1:3545727/128‑1 (MQ=255)
                              tactCCTATTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                           <  1:1832955/125‑1 (MQ=255)
                                 tCCTTTTATAGTTGACCATAATTTAAGATAGAGAATAGCAGAAGCTCTTAACAACTTAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCTccat                                                                                        <  1:36051/128‑3 (MQ=255)
                                  ccatttATAGTTGACCAAAAAATAAGATAGAGCAAAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                       <  2:2874644/125‑4 (MQ=255)
                                  ccTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                       <  2:2105913/128‑4 (MQ=255)
                                    ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                     <  2:160807/128‑1 (MQ=255)
                                    tattaaacttGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                     <  1:1706255/120‑1 (MQ=255)
                                     tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCCACCATTAt                                                                                    >  1:3018532/1‑128 (MQ=255)
                                     tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                    <  1:2491925/128‑1 (MQ=255)
                                     tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTAt                                                                                    >  1:190461/1‑128 (MQ=255)
                                     tatataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                    <  1:1592909/126‑1 (MQ=255)
                                       tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCCCCCATTATgg                                                                                  >  2:329373/1‑128 (MQ=255)
                                        ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 <  2:433534/128‑1 (MQ=255)
                                          aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   >  1:3685798/1‑124 (MQ=255)
                                              gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGttt                                                                           >  2:932222/1‑128 (MQ=255)
                                                  aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAATAAGCAATTAAACTCACAAAAACTCACACATTAATGTGAGTTTaaca                                                                       >  1:796000/1‑126 (MQ=255)
                                                  aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTTAACACACAAAAACTCACCCATTATCGTGAGTTTaaaa                                                                       >  2:3631930/1‑128 (MQ=255)
                                                   aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:2617592/128‑1 (MQ=255)
                                                   aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:2212632/128‑1 (MQ=255)
                                                   aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAAACAATATAGTGAGTTTaaaaa                                                                      >  1:1231743/1‑128 (MQ=255)
                                                     aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  1:513811/128‑1 (MQ=255)
                                                     aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  2:2735860/128‑1 (MQ=255)
                                                      aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:3999125/125‑1 (MQ=255)
                                                         aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   >  2:3137489/1‑109 (MQ=255)
                                                         aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                >  1:2163786/1‑128 (MQ=255)
                                                          aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCa                                                               >  2:3489186/1‑128 (MQ=255)
                                                           gaaagagCATAGCAGAAGCTCTTAAAAACTAAAATAAAATAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                              <  2:787459/125‑1 (MQ=255)
                                                             tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATTTAAAATGCAATTAAGCACACAAAAACTCACCCATTAGGGTGAGTTTAAAAATTATTCaaaaa                                                           >  2:732739/1‑128 (MQ=255)
                                                              agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                           <  1:866246/128‑1 (MQ=255)
                                                               gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 <  1:726647/105‑1 (MQ=255)
                                                               gagCATAGAAGAAGCTCTTAACAACTAAACTAAACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGGAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          <  2:650383/128‑1 (MQ=255)
                                                                agCATAGCAGAATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         <  1:1155450/128‑1 (MQ=255)
                                                                agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         <  2:1385520/128‑1 (MQ=255)
                                                                 gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                        >  1:43860/1‑128 (MQ=255)
                                                                 gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTg                                                       >  1:2759980/1‑128 (MQ=255)
                                                                 gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATAATATTTTTTTTTAATATAAAAATCAATTAAACAAACAAAAACTCACCCATTATGCTGAGTTTAAAAATTATTCAAAAAttt                                                        >  2:3807591/1‑128 (MQ=255)
                                                                    tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  2:3232424/128‑1 (MQ=255)
                                                                    tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  2:2551324/128‑1 (MQ=255)
                                                                        aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                 <  2:1956952/128‑1 (MQ=255)
                                                                              tctTAACAACTAAAATACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATAATTAAAAAATTTGAATATTTTAAAt                                           >  1:624492/1‑128 (MQ=255)
                                                                                ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                         <  1:3956516/128‑1 (MQ=255)
                                                                                   acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                       <  2:2221574/128‑1 (MQ=255)
                                                                                   acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                      <  2:301603/128‑1 (MQ=255)
                                                                                    caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   <  1:1376750/82‑1 (MQ=255)
                                                                                    caacTAAACTACACTCGATTATTTAATTATATTTTTTATTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTCAGTTTAAAAATTATTCAAAAAATTGATTAATTAAAATCAATTg                                     >  2:3073240/1‑128 (MQ=255)
                                                                                     aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    <  1:1074300/128‑1 (MQ=255)
                                                                                     aactaaactaCACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   >  2:1279127/1‑128 (MQ=255)
                                                                                      actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   >  1:241651/1‑128 (MQ=255)
                                                                                      actaaactaCACTAGATTTTTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  2:2299484/128‑1 (MQ=255)
                                                                                      actaaaattAACTCNANTATTTAATTATATTTTTTTTTTATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  2:3689654/128‑1 (MQ=255)
                                                                                          aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                               >  1:3329119/1‑128 (MQ=255)
                                                                                          aaataCACTATATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATCAATTGAAGCTc                               <  1:2917632/125‑1 (MQ=255)
                                                                                            ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAATCTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATCAATTGAAGCTCCa                             <  2:2366019/128‑1 (MQ=255)
                                                                                            ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATAAGATTATTTTAAATCAAATGAAGCTCCa                             >  1:2712315/1‑128 (MQ=255)
                                                                                             taCAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                            <  2:501004/128‑1 (MQ=255)
                                                                                              acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGTAGCTCCAGc                           >  1:3183967/1‑128 (MQ=255)
                                                                                              acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           <  1:4020658/128‑1 (MQ=255)
                                                                                              acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           >  2:1214386/1‑128 (MQ=255)
                                                                                              acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           >  2:1859610/1‑128 (MQ=255)
                                                                                                acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                         >  2:33885/1‑128 (MQ=255)
                                                                                                acTCGAATATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTATAAAAATTATACAAAAATTTGATAATTTTAAATCAATTGAATCTCAAGCtt                         >  2:2683881/1‑128 (MQ=255)
                                                                                                   cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTTAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAATCTCCAGCTTCaa                      >  2:2016301/1‑128 (MQ=255)
                                                                                                    gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGATTCAAc                     >  1:2551465/1‑128 (MQ=255)
                                                                                                      ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTa                   >  1:2271532/1‑128 (MQ=255)
                                                                                                        attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                >  2:575693/1‑81 (MQ=255)
                                                                                                          ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               <  1:3774028/128‑1 (MQ=255)
                                                                                                           tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa              <  2:1683633/128‑1 (MQ=255)
                                                                                                           tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACACACCCATAATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCCGCGTCAACTAAATTa              >  2:1516540/1‑128 (MQ=255)
                                                                                                               tataTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCtt          >  2:1045861/1‑128 (MQ=255)
                                                                                                                atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:3935450/1‑128 (MQ=255)
                                                                                                                atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:1137078/1‑128 (MQ=255)
                                                                                                                 tatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTc        >  2:1745932/1‑128 (MQ=255)
                                                                                                                 tatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTc        >  1:341525/1‑128 (MQ=255)
                                                                                                                  atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTaa                  >  1:957677/1‑117 (MQ=255)
                                                                                                                  atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               <  2:861024/120‑1 (MQ=255)
                                                                                                                    ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt     <  2:3874407/128‑1 (MQ=255)
                                                                                                                       tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct  <  2:241651/128‑1 (MQ=255)
                                                                                                                              |                                                                                                                         
TATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484667‑484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: