Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.67.8% 1454.3 / inf 1126intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (142/218);  new base . (539/219);  total (683/443)
Fisher's exact test for biased strand distribution p-value = 9.83e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1367 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTAAAAGAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGAAAAAA‑TATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377
                                                                                                                                   |                                                                                                                                 
 taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCtt                                                                                                                                  >  2:2295147/1‑128 (MQ=255)
 taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCtt                                                                                                                                  >  2:1527808/1‑128 (MQ=255)
   atattttcgtttaTTAATTTATCGCCCTTTTATGAATTAGTTATAAAATAAAAATATTATATTTTTATATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTTAACTTGTAAAAAAAAGCtttt                                                                                                                                <  2:3400990/116‑1 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCtttt                                                                                                                                >  1:2176066/1‑128 (MQ=255)
     atTTAGTTTTAATAATTATTTTCTATTTTATTAATTATTAACTAAATAAAATTATTATATTTATATTTTTTTTTTTTTTT‑TTCAA‑A‑TTTTTAAAAAAATAA‑TTTTAACTTTTAAAAAATAGCtttttt                                                                                                                              <  2:459356/128‑1 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttttt                                                                                                                         >  1:1216960/1‑128 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttttt                                                                                                                         >  2:286094/1‑128 (MQ=255)
          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑ATTGAACTTGTAAAAAATAGCatttttttttt                                                                                                                         >  1:886030/1‑128 (MQ=255)
            ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATATCttttttttttttt                                                                                                                       >  2:1186809/1‑128 (MQ=255)
               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTTTAAAAAATAGCttttttattttttttt                                                                                                                    >  1:1826004/1‑128 (MQ=255)
               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGAtttttttttttttttt                                                                                                                    >  2:854600/1‑128 (MQ=255)
               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGAttattttttttttttt                                                                                                                    >  1:2640687/1‑128 (MQ=255)
                 tAATTTATCGCACTTGTATGAATTATTAATAAAATAAAAATATTATATTTTTATATATTTTTTTTATA‑TTCAA‑A‑TTTTTAAAAAATTAA‑TTTTAAATTGTAAAAAATATCttttttttttttttttat                                                                                                                  <  2:161590/128‑3 (MQ=255)
                   aTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttttttttttataa                                                                                                                >  2:597346/1‑124 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttttatttataaatg                                                                                                               >  1:942054/1‑121 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataa                                                                                                               >  1:1889743/1‑127 (MQ=255)
                       atagCACTTTAATTAATTATTAAGAAACAAAAACTATTTTCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTtataatgg                                                                                                            <  1:1991286/125‑5 (MQ=255)
                       aTCGCACTTGTTTGAATTAGTAAGAAACTAAAACTATTATCTTTTTATATATTTTTTGTAAA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCttttttttttttttttataatgga                                                                                                            <  1:3604949/128‑9 (MQ=255)
                       aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataaatg                                                                                                            >  1:468427/1‑124 (MQ=255)
                        tcgaaattGTTTTAATTAGTAATAAAATAAAAATATTTTATTTTAAGATATTTATTTTATA‑TCCAA‑A‑ATTTTAAAAAATTAA‑ATTGAAATTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATgg                                                                                                          <  2:2783248/122‑1 (MQ=255)
                        tCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTATTTTTTTTTTTTTTtataattta                                                                                                           >  2:3784514/1‑123 (MQ=255)
                          gcaattgtttttattattAATAAAATAAAAATATTATTTTTTTTTTTATTTTTTGTTTT‑TTTAA‑A‑TTTTTTAAAAATTTA‑TTTTTAATTTTAAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGaaa                                                                                                       <  2:2383633/116‑1 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtaatttaaatat                                                                                                      >  2:3721785/1‑118 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTaaattaaaa                                                                                                      >  2:3239837/1‑121 (MQ=255)
                              ttGTATGAATTAGTAGGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGTAAAtata                                                                                                   >  1:3722412/1‑128 (MQ=255)
                                 tattaattattaatAAAATAAAAATATTATTTTTTTTTATATTTTTTGTTTATTGAAA‑A‑TTTTAAAA‑AATTAA‑TTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                               <  1:3102406/128‑1 (MQ=255)
                                 tATTAATTAGTAAGAAAATTAAAATATTATCTTTTTATATTTTTTTTTTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                               <  1:2958500/128‑1 (MQ=255)
                                 tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGTAAATATAAAt                                                                                                >  2:1012859/1‑128 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATTAAAATAAAAATTTTAgta                                                                                         >  1:3155713/1‑126 (MQ=255)
                                         aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATTTAAATATAAATTTTAgtt                                                                                         >  2:3880345/1‑126 (MQ=255)
                                         aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTCAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAATTATAAATTTTAGGAAt                                                                                       >  2:4029396/1‑128 (MQ=255)
                                               taaataaaaataTTATATTTTTATTTATTTTTTGTATA‑TTCAA‑A‑TTTTTAAAAAATTAA‑TTTTAAATTTTAAAAAATA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgt                                                                                  <  2:1469391/124‑1 (MQ=255)
                                               aaaataaaaattTTTTATTTTTTTATTTTTTTTTTTTA‑TTTAA‑A‑TTTTTAAAAAATTTA‑TTTTAAATTTTATAAAAT‑TTATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                                                   <  1:654049/128‑1 (MQ=255)
                                                taataaaaataTTATTTTTTTTTTATTTTTTTTTATA‑TTTAA‑ATTTTTAAAAAAATAAA‑TTTTAA‑TTTTATAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                                                 <  1:693005/125‑1 (MQ=255)
                                                aactaaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTATTTTTTTTTTTAAAATTTAAATATAAATTTTAGttttttttttt                                                                                >  2:2123505/1‑127 (MQ=255)
                                                 actaaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAG‑ATTTTTTTTTTTTTTTTTAATAATTTATAAATAATTTTTAGTTATTttgtt                                                                                 >  1:142961/1‑128 (MQ=255)
                                                   taaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTATA‑AAT‑TAAATATAAATTTTAGTTATTttgtttta                                                                              >  2:131718/1‑125 (MQ=255)
                                                   taaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATGTAAtttttttta                                                                              >  2:2717354/1‑125 (MQ=255)
                                                    taaaataTTAATTTTTTACTTTTTTTTTGTTTA‑TTCAATA‑TTTTAAAA‑AAAAAA‑ATTCAAATTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                                                            <  1:1145545/127‑1 (MQ=255)
                                                    aaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAA‑AAAAAATTTAAGTATtttttttttat                                                                            >  1:3797339/1‑123 (MQ=255)
                                                    aaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGGAAATTTAAATTTTAGTTATTTttttgtat                                                                             >  2:929701/1‑126 (MQ=255)
                                                    aaaactaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAAAGTAAATATAAATTTAATTAATTTTTTTTTCTa                                                                            >  1:411046/1‑128 (MQ=255)
                                                     aaaataTTATATTTTTATTAATTTTTTTTATA‑TCTAA‑A‑TTTTTAAAAAATTAA‑TTTTAATTTTTAAAAAAAA‑‑‑‑TCATTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                                                          <  2:2052510/128‑1 (MQ=255)
                                                      atttattatTTTTTTTTTTTTTTTTTTTATT‑TTAAA‑A‑TTTTTAAAAAATAAA‑TTTTAACTTTTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                                                           <  1:3670421/125‑1 (MQ=255)
                                                      aaatattatTTTTTTATATATTTTTTTTTTA‑ATTAA‑A‑TTTTTAAATAATTAATTTTTAATTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                                                            <  1:1514400/125‑1 (MQ=255)
                                                       actaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTATTTATttttttttaaaat                                                                          >  1:1751412/1‑121 (MQ=255)
                                                       aatattatTTTTTTATTTATTTTTTTTATA‑TTAAA‑A‑TTTTTAAAAAATTAA‑TTTTAAATTTTAAAAAATA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                                                          <  1:812554/126‑1 (MQ=255)
                                                        ctaTTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGT‑AAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTAAGTTATTTTTTTTTATAATT‑a                                                                       >  2:691521/1‑128 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTGTTTTCTAATT‑At                                                                      >  1:1391883/1‑128 (MQ=255)
                                                         tattatCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTATTAAAATGGAATTATAAATTTTAGTTATTTTTTTTAATAAGT‑At                                                                      >  1:532851/1‑128 (MQ=255)
                                                          attttattttttTATTTTTTTTGTATA‑TTAAA‑A‑TTTTTTAAAAATTAA‑TTTGAAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑At                                                                      <  2:2601677/122‑1 (MQ=255)
                                                           ttatCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATtttttttttaaattatc                                                                      >  2:2500349/1‑117 (MQ=255)
                                                           ttatCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTGTATAATT‑Ata                                                                     >  1:2796225/1‑127 (MQ=255)
                                                             ttttttttATATTTTTTTTTTATA‑TTCAA‑A‑ATTTTAAAAAATTAA‑ATTTAAATTTTAAAAAAAATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCt                                                                    <  1:3385334/125‑1 (MQ=255)
                                                                  tttATATATTTTTTTTATA‑TTAAA‑A‑TTTTTAAAAAATTAA‑TTTGAAAATTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTaaaaaa                                                              <  1:3441967/128‑1 (MQ=255)
                                                                   tttgATATTTTTTTTTTA‑TTTAA‑A‑TTTTTAAAAAATTAA‑TTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTaaaaaaa                                                             <  1:1044887/125‑1 (MQ=255)
                                                                    tattttttttttttATT‑TTTAA‑A‑TTTTTTAAAAATTTA‑TTTTTAATTTTTAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAAt                                                            <  1:597525/122‑1 (MQ=255)
                                                                    tAGATATATTTTTTATA‑TTTAA‑A‑TTTTTAAAAAATTAA‑TTTTAAATTGTAAAAAATAG‑‑‑ATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATa                                                           <  1:270607/128‑1 (MQ=255)
                                                                     atttatTTTTTTTATA‑TTGAA‑A‑TTTTTAAAAAATTAA‑TTTTAACTTTTAAAAAATA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAg                                                          <  2:3332279/125‑1 (MQ=255)
                                                                     agttatTTTTTTTATT‑TGTAA‑A‑TTTTTAATAATTTAA‑TTTGAATATGTTAAAAATA‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAg                                                          <  1:244935/125‑1 (MQ=255)
                                                                     aGATATTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAATTATTTTTTTTTATAATT‑ATAAAAAAAAATa                                                           >  1:1584614/1‑128 (MQ=255)
                                                                       atatTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTAGTAATTTTTTTTTATAATT‑ATATAAAAAAATaaa                                                         >  2:1956974/1‑126 (MQ=255)
                                                                        gtatttagatata‑TTCAA‑A‑TTTTTAAAAAATTAA‑TTTTTAATTTTTAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGaa                                                        <  1:3850715/119‑1 (MQ=255)
                                                                         atTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTGTAAAATT‑ATAAAAAAAAATATaaa                                                       >  2:776089/1‑128 (MQ=255)
                                                                         atTTTTTGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATATTTTAAATATAAATTTTAGTTATTTTGTTTTATAATT‑ATCTAAAAAAATATaaatg                                                     >  1:3040116/1‑126 (MQ=255)
                                                                           tttttGTTTA‑TTCAA‑A‑TTTTTAAAAAATTAA‑TTTTAACTTTTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAg                                                      <  1:2051440/128‑1 (MQ=255)
                                                                           tttttGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTTTTGTATAAGT‑ATATAAAAAAATAaaaatta                                                    >  1:1283845/1‑125 (MQ=255)
                                                                            ttttGTATA‑TTCAA‑A‑ATTTTAAAAAATTAA‑TTTTAAATTTTAAAAAATA‑C‑‑GTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGaa                                                   <  1:3487612/128‑1 (MQ=255)
                                                                             ttttttttattaaa‑a‑TTTTTTAAAAATTAA‑TTTTAAATTTTATAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGa                                                    <  1:1009766/116‑1 (MQ=255)
                                                                              ttGTTTA‑TTCAA‑A‑TTTTTAAAAAATTAA‑TTTTTAATTTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGaaa                                                  <  1:2298884/128‑1 (MQ=255)
                                                                              ttGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATTTAAATATAAATTTTATTTATTTTTTTTTATAATT‑TAAAAAAAAAATAaaaataaa                                                   >  1:2389828/1‑124 (MQ=255)
                                                                              ttGTATA‑TGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATGTAAAAATAAATTTTATTTATTTTTTTTTATAATT‑ATATAaaaaaaaaaaaaataa                                                   >  2:1253457/1‑124 (MQ=255)
                                                                               tttata‑TTCAA‑A‑TTTTTAAAAAAATAA‑TTTGTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGaaaaa                                                <  2:363030/126‑1 (MQ=255)
                                                                               tGTATA‑TTTAA‑T‑TTTTTAAAAAATTAA‑TTTTAAATTTTATAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGaaaa                                                 <  1:3915845/128‑1 (MQ=255)
                                                                                  ata‑tGTAA‑A‑TTTTTAAAAATTTTA‑TTTTTAATTTTTAAAAAT‑‑TTGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGAAAAAa‑t                                             <  1:750438/128‑1 (MQ=255)
                                                                                   attttaaa‑a‑TTTTTAAAAAAATAA‑TTTTTAATTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGAAAAAa‑t                                             <  1:1349855/122‑1 (MQ=255)
                                                                                    a‑tGCAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATATTTTAAATAAAAATTTTTGTTTTTTTTTTTTATAATT‑TAATAAAAAAATATAAAGTaaaaatat                                             >  2:3190035/1‑125 (MQ=255)
                                                                                      tgtaa‑a‑TTTTTTAAAAATTTA‑TTTGAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGAAAAAA‑TAtt                                          <  2:1889743/125‑1 (MQ=255)
                                                                                        cAA‑A‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGAAAAATTAAATTTT‑TGTTTTTTTTTTTTTAATT‑TAATAAAAAAAAAAAAATAAAAAAATTATTGt                                        >  2:3456213/1‑128 (MQ=255)
                                                                                          a‑a‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTATAAGT‑ATATAAAAAAATAGAAAGTAAAAAA‑TATTTTTaa                                     >  2:107971/1‑128 (MQ=255)
                                                                                            a‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTGTTTTATAAGT‑ATAAAAAAAAATATAATATAAAAAA‑TATTGTTaaa                                    >  1:2868594/1‑128 (MQ=255)
                                                                                            a‑TTTTTAAAAAATTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                                                     >  1:3239166/1‑81 (MQ=255)
                                                                                                 ttaaaaaaTTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATTATGGAAATATTAATTTTAGGTATTTTTTTTTATAATT‑ATAAAAAAAAATATAAAAGAAAAAA‑TATTTTTAAAAATa                                >  1:2256513/3‑128 (MQ=255)
                                                                                                  taaaaaaTTAA‑TTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAAAATAAATTTTATGTATTTTTTTTTAAAATT‑ATAAAAAAAAATAAAAATTAAAAAA‑TATTTTTaaaaaaa                                >  2:2952044/2‑125 (MQ=255)
                                                                                                  taaaaaaTTAA‑TTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTGTATAAGT‑ATATAAAAAAATTAAAAGAAAAAAA‑AATTGTTAAAAGTaa                               >  2:3042208/2‑128 (MQ=255)
                                                                                                     aaaattaa‑ttTTTAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGAAAAAA‑TATTGTTAAAAGTAATa                             <  1:2121555/126‑1 (MQ=255)
                                                                                                     aaaattaa‑ttTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGGAAATATAAATTTTATTAAATTTTTTGAAATATT‑AAATAAAAAATTAAAAATTAATAAA‑AATTGTTAAATGTAATa                             >  2:1818324/3‑128 (MQ=255)
                                                                                                     aaaattaa‑ttTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATATTTGAAAAATAAATTTTATTTTTTTTTTTTTCTAATT‑TTATAAAAAATTATAAAATAAAAAA‑TATTTTTAAAATTataaata                          >  1:1652359/3‑122 (MQ=255)
                                                                                                       aattaa‑ttTGAACTTGTAAAAAATAGC‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑TTCTAAAAAAATATAAAGGAAAAAT‑TATTTTTAAAAGTataac                            >  1:331461/1‑124 (MQ=255)
                                                                                                        attaa‑ttTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TTTAAATA‑AAATTTTATGATTTTTTTTTTAAAATTAATATAAAAAAATAAAAATTAAAAAA‑TATTTATAAAATAAataata                          >  2:3113987/1‑128 (MQ=255)
                                                                                                        attaa‑ttTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGGAATTATAAATTTTTGTTTTTTTTTTTTATAAGT‑ATATAAAAAAATATAAAGTAAAAAA‑ATTTGTTAAAATTAataata                          >  1:3539336/1‑128 (MQ=255)
                                                                                                         ttaa‑ttTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAT‑ATTTAAAAATAAATTTTTTGTATTTTTTTGTTAAATT‑ATATAAAAAAATAAAAAATAAAAAA‑AATTTTTAAATTTAATTATaaa                        >  2:1728481/1‑128 (MQ=255)
                                                                                                         ttaa‑ttTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTAATTTTTTTTTCAAATT‑ATCTAAAAAAATAAAAAGAAAAAAA‑TATTTTTAAAAGTAAAAATaaa                        >  2:1409624/1‑128 (MQ=255)
                                                                                                            a‑ttTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTGTCTAATT‑ATATAAAAAAATATAAATTAAAAAA‑TTTTTTTAAAATTAATAATAAAat                      >  1:3988972/1‑128 (MQ=255)
                                                                                                                tGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTATAAAATGTAAATATAAATTTTAGTTTTTTTTTAGTAAAATT‑AAAATAAAAATTAGAAATGAAAAAA‑TATTGTTAAAATTATTAATAAAataa                    >  2:2218268/1‑126 (MQ=255)
                                                                                                                tGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTGTGTAATT‑ATATAAAAAAATATAAAGAAAAAAA‑TATTTTTAAAATAAATCATAAAatttt                   >  2:3926296/1‑126 (MQ=255)
                                                                                                                  aaCTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTATTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGAAAAAA‑TATTGTTAAAAGTAATACTAAAATTAttt                 <  1:3203490/128‑1 (MQ=255)
                                                                                                                  aaCTTGTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTTATAATTGAATTATAATTTTTAGTTTTTTTTTTTTATAATT‑TTATAAAAAAATTATTATGAAAAAA‑ATTTTTAAAAATTAATAATAAATtttttt                 >  2:3531383/1‑128 (MQ=255)
                                                                                                                   aCTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAATTGTAAATAAAAATTTAAGTTATTTTGTTGTCTAATT‑ATAATAAAAAATAAAAAGGAAAAAA‑TTTTGTTAAATTTAATAATAAAATAATTTa                >  1:2570674/1‑128 (MQ=255)
                                                                                                                         ttaaaaattttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGAAAAAA‑TATTGTTAAAAGTAATACTAAAATTATTTAATGt            <  2:529146/118‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATA‑TGCAA‑A‑TTTTAAAAGAATTAA‑TTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGT‑ATCTAAAAAAATAGAAAGGAAAAAA‑TATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAC  >  CP016816/165123‑165377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑