Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 203,911 (T)16→17 intergenic (+295/+163) JCVSYN2_00845 → / ← JCVSYN2_00850 lipoprotein/inorganic diphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816203,8951.T83.5% 567.6 / 107.7 207intergenic (+279/+179)JCVSYN2_00845/JCVSYN2_00850lipoprotein/inorganic diphosphatase
Reads supporting (aligned to +/- strand):  ref base . (16/18);  new base T (163/9);  total (179/28)
Fisher's exact test for biased strand distribution p-value = 1.47e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 288 total aligned reads displayed.

TTTTAATTTTTTTTATAAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAA  >  CP016816/203767‑204008
                                                                                                                                              |                                                                                                                                    
ttttATTTTTTTTTATAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCATAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑g                                                                                                                                       >  1:2151710/1‑128 (MQ=255)
 tttAATTTTTTTTATAAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑g                                                                                                                                       >  1:374123/1‑128 (MQ=255)
  ttAATTTTTTTTATAAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑t                                                                                                                                >  1:1988890/1‑128 (MQ=255)
  ttAATTTTTTTTATAAAAAAAAATAAAAATTTTTAATTTAAATCCATTTTTAAAAAATCCATTTTTCTTGGCTTTTTATTATACTTATCAACATTACAACCCTTTCAAAGGGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑t                                                                                                                                <  1:3495809/128‑1 (MQ=255)
     tttttttttATAAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAANTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑tttt                                                                                                                             >  2:3238531/2‑128 (MQ=255)
        ttttttATAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑ttt                                                                                                                      >  1:2275696/1‑128 (MQ=255)
         tttttATAAAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tt                                                                                                                       >  1:1000264/1‑128 (MQ=255)
         tttttATAAAAAAAAATAAAAATTTTTCATTTAAAGCAATTTGTAAAAAAGCATATTTTCTAGGGTTTTTATTATAGTTATCAAAATTAATACTCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑ttt                                                                                                                      >  1:3172539/1‑128 (MQ=255)
          ttttATAAAAAAAAATAAAAATTTTTTATTTAAATAAATTTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTTTTAATTTTAAAAATTAAAAAGTTTTAAATTTTTATTTTTTATGT‑‑‑tt‑‑‑‑‑‑‑‑t‑‑‑‑‑‑ttttt‑‑‑tttt                                                                                                                     <  2:2396038/128‑1 (MQ=255)
           tttATAAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTTTATTTTTTAGGC‑‑‑GT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑ttttt                                                                                                                    >  2:447571/1‑128 (MQ=255)
           tttATAAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCATATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGAGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑ttttt                                                                                                                    >  2:3690079/1‑128 (MQ=255)
              taaaaaaaaaaaTAAAAATTTTTAATTTAAACCTATTTTTTAAAAATACTTTTTTATTTTCTTTTTTTTTTATTTATAAAAATTAAAACTGTTTTAAATTTTTTTTTTTTTTG‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑ttttttt                                                                                                                  <  2:1633869/125‑1 (MQ=255)
               taAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCATATTTTATAGGATTTTTTTTATACTTATAAAAATTACAAAGATTTAAAATTTTTTTTTTTTGGA‑‑‑Tt‑‑‑‑‑‑‑‑t‑‑‑‑‑‑ttttt‑‑‑ttttttttt                                                                                                                >  2:542794/1‑128 (MQ=255)
                ataaaaaaaaTTAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTATGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑ttttttttt                                                                                                                >  2:3976717/3‑128 (MQ=255)
                 aaaaaaaaaaaaaaaaTTTTAATTTTAATCAATTTTTAAAAAACGCTTTTTTTTTGGTTTTTTTTTTTAATTTTAAAAATTAAAACGGTTTAAAATTTTTTTTTTTTGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tttttttttt                                                                                                               <  2:3939897/128‑1 (MQ=255)
                   aaaaaaaTTAAAATTTTTTATTTAAATCGATTTTTAAAAAATGATTTGTTATTTGGTTTTTATTTTAAATATAAACATTACAACGCTTTCAATGTGTTTTTTTTTGGT‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tttttttttttt                                                                                                             <  2:210949/128‑2 (MQ=255)
                   aaaaaaaTAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACTCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tttttttttttt                                                                                                             >  1:947607/1‑127 (MQ=255)
                    aaaaaaTAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACTACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑ttttttttttttt                                                                                                            >  1:962062/1‑126 (MQ=255)
                    aaaaaaTAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑ttttttttttttt                                                                                                            >  2:1340842/1‑126 (MQ=255)
                    aaaaaaTAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTGCTAGGCTTTTTTTTATACTTTTCAACATTACAACTCTTTCAAAGTGTATTTTTGAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttg‑‑‑ttttttttttttt                                                                                                            >  1:701120/1‑126 (MQ=255)
                     aaaaataaaaatTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAATGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tttttttttttttt                                                                                                           >  1:3042683/1‑125 (MQ=255)
                     aaaaataaaaatTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tttttttttttttt                                                                                                           >  1:568423/1‑125 (MQ=255)
                     aaaaataaaaatTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tttttttttttttt                                                                                                           >  2:3494162/1‑125 (MQ=255)
                            aaaatTTTTCAATTAAAACCATTTTTAAAAAATCCATTTGTTTACGCTTTTTATTTTTCTTATAAAAAATACAACGCTTTCAAACTTTTTTTTTTATGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tttttttttttttttgtaaaa                                                                                                    <  1:3278811/128‑11 (MQ=255)
                             aaatTTTTTTTTTATACCAATTTTTTAAAAAGCCTATTTTTTCGGTTTTTTATTATAATTTAAAACATTACCCCGCTTTAAAATTGTATTTTTTTGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑ttttt‑‑‑tttttttttttttttgtaaaaa                                                                                                   <  2:423908/128‑12 (MQ=255)
                             aaatTTTTAATTTAAACTCATTTGTAAAAAATCCTTTTTTTTAGGTTTTTTTTTATAATTATCAAAATTAAAACCCTTTCAAATTGTTTTTTTTTGGC‑‑‑Tt‑‑‑‑‑‑‑‑t‑‑‑‑‑‑ttttt‑‑‑ttttttttttttttgtaaaaat                                                                                                   <  2:2813716/128‑12 (MQ=255)
                                tttttCATTTAAAGCCATTTGTAAAAAAGCATATGTTATAGGATTTTTATTATACTTATCAACATTAAAACGATTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑TTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAAAAAAATTa                                                                                        >  1:1310050/1‑128 (MQ=255)
                                       tttAAATCCATTTTTAAAAAATCCTATTTTTTTTGTTTTTTTTTTTTATTATAAAAATTAAAAATCTTTAAAATTTTATTTTTTAGTCTTTT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGAt                                                                                    <  1:983377/128‑1 (MQ=255)
                                        ttAAATCCATTTGTAAAAAAGCCTATGTTCTTGGCTTTTTATTTTAATTTTCAACATTACAACGGTTTCAAATTGTATTTTTTAGGG‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGAtt                                                                                   <  2:3133992/128‑1 (MQ=255)
                                           aaGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTGAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GT‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATATTTGGa                                                                               >  1:3271219/1‑128 (MQ=255)
                                           aaGCCATTTGTAAAAAAGCATATGTGTTAGGATTTTTTTTATAATTATCAACATTACAACGCTTTAAAATTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTgg                                                                                <  1:3105055/128‑1 (MQ=255)
                                                aTTTTTAAAAAATCCTATTTTATTGGTTTTTTTTTAAAATTATAAAAAATACAACTGTTTAAAATTGTATTTTTTTGGG‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGATCa                                                                            <  2:1988890/128‑1 (MQ=255)
                                                 tttGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAGATTTGGAACaa                                                                           >  1:2998601/1‑128 (MQ=255)
                                                   tttAAAAAATCATATTTTCTAGGATTTTTATTATAATTAACAAAATTAAAACCCTTTAAAATTTTATTTTTTAGGCTTTTT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGATCAAcc                                                                        <  1:3432220/126‑1 (MQ=255)
                                                      aaaaaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAATTAATATTTTGATcaaacaaa                                                                     >  1:754377/1‑128 (MQ=255)
                                                        aaaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGAAAAACCaaa                                                                     >  1:3073351/1‑128 (MQ=255)
                                                        aaaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAGATTTTGATCAACAaaaa                                                                    >  1:3593433/1‑128 (MQ=255)
                                                        aaaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAGATTTTGATAACACaaaa                                                                    >  2:3551251/1‑128 (MQ=255)
                                                        aaaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GT‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAAATTTTGTTAAACAaaaaaaa                                                                 >  1:2193169/1‑125 (MQ=255)
                                                         aaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATATTTGGAACAACCaaaa                                                                    >  2:3538067/1‑128 (MQ=255)
                                                         aaaGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑TAAAATAAAAGTAATATTTGGTTAAACAaaaaa                                                                   >  1:885397/1‑127 (MQ=255)
                                                            gCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTTGATCAACAaaaaaatt                                                                >  1:1181626/1‑124 (MQ=255)
                                                              cTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAATTATGATTTGGATCAAACaaaaaaatt                                                               >  1:208574/1‑123 (MQ=255)
                                                                tATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCATAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAATATTAGGATCAAACAAAACATATaaaa                                                       >  1:1955982/1‑128 (MQ=255)
                                                                tATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAAGATTTTGAAAAACAAAACtattata                                                          >  1:426806/1‑124 (MQ=255)
                                                                tATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAAATAATATTTTCATCAACAAAAAATATATCa                                                         >  2:3742997/1‑128 (MQ=255)
                                                                  tGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAATAAAATTTTGATCAACAAAAAAAATAACaaa                                                       >  2:3863129/1‑128 (MQ=255)
                                                                  gaacatAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAATTTATTATTTGTATAAACAAAAAATAAATCaaa                                                       >  2:97053/6‑128 (MQ=255)
                                                                      cTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGATAAAACACTACTTTACaaaaat                                                    >  1:3611821/1‑127 (MQ=255)
                                                                       tatgCTTTTTATTATAATTATCAACATTAAAAAGATTTAAAATTGTATTTTTTTGGC‑‑‑TTTTTTTTTCT‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGATCAACCAAAACTATATCaaa                                                       <  1:4029398/125‑1 (MQ=255)
                                                                       tAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑TAAAATAAAATTATATTTTTGTTCAACCAAAATAATATAaaaaaat                                                   >  1:2421145/1‑127 (MQ=255)
                                                                       tAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTGG‑‑‑‑‑‑AAAAATAAATATAAATTTTGTATCAACACAAAATATaaaaaaaaaa                                                   >  1:3778504/1‑128 (MQ=255)
                                                                       tAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAATTAAATTTTGGATGAACAAAAACTATATaaaaaaaaat                                                 >  2:3400056/1‑126 (MQ=255)
                                                                        aGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑TAAAATAAAAGTTATATTTGTTTAAAACAAAACTATTTAAAAAAAAATa                                                >  1:2400032/1‑128 (MQ=255)
                                                                              tttATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑TTTTTTTATTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAAATAAAAATTTGAAAAAAAAAAAAAAAAAAAAAAAAAATaaaaaa                                           >  1:2688198/1‑128 (MQ=255)
                                                                                  ttatACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAATTTTTGTATAAAAAAAATATATTtaaaaaaaattaaaaaaaat                                        >  1:2955698/1‑128 (MQ=255)
                                                                                  ttatACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAAATATTAATTGGTTAACCCAAAACTTTATAAATAAAATTaaaaaaaaa                                        >  1:3341268/1‑127 (MQ=255)
                                                                                   tataCTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTATAATTTGGATAACAAAAAAATTATAAAAAAAATTtaaaaaaaaa                                       >  1:332444/1‑126 (MQ=255)
                                                                                    ataCTTATCAACATTACAACGCTATCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAAATAAAATTTTTATCAAAAAAAAAATAAAAAAAAAAATTAAAAAAAAtag                                      >  2:3052758/1‑128 (MQ=255)
                                                                                     ttattATCAACATTAAAAATCTTTAAAAGTTTATTTTTTATGT‑‑‑TT‑‑‑‑‑‑‑‑GT‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAAtagttta                                  <  2:3080491/125‑1 (MQ=255)
                                                                                     taCTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATATTTTGAAAAACCAAAAAAATAAAAAAAAAATTaaaaaaaaaattt                                    >  2:3325722/1‑123 (MQ=255)
                                                                                     taCTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAAAATTTTTATCAACCAAAAAAATAAAAAAAAAATTAAAAAAAAtagttt                                   >  1:1511546/1‑128 (MQ=255)
                                                                                      aCTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAATATTTTGAAAAACAAAAAAAATAAAAAAAAAATTAAAAAAAAtaat                                     >  1:2492997/1‑126 (MQ=255)
                                                                                      aCTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATAAATGGAAAACCAAAACAAATAAATAAAAAATTaaaaaaaaaattt                                    >  1:1238461/1‑123 (MQ=255)
                                                                                        ttATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAATATTTGGTTAAACCAAAAATTTTTAAAAAAAATTAAAAAAAAtattttt                                  >  1:3952581/1‑127 (MQ=255)
                                                                                        ttATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTTTTTA‑‑AAAAAAAAAAATAAGTTTTTGAACAAAAAAAAATATAAAAAAAAAATTAAAAAAAAtatttta                                  >  1:3117792/1‑128 (MQ=255)
                                                                                         tATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATTAAATTAATATTTTTTAAAAACAAAAATATTTAAAAAATATTAAAAAAAAtatttta                                  >  1:1658775/1‑128 (MQ=255)
                                                                                         tATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATATTTGGATAAACAAAAAATATATAAAAAAAATTAAAAAAAAtttttta                                  >  1:1114721/1‑128 (MQ=255)
                                                                                         tATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTTTTTTTAAAAAATAAAATAAAGATTTGGATAAAAAAAAAATAATTAAAAAAAATTAAAAAAAAaaattt                                   >  1:1919504/1‑128 (MQ=255)
                                                                                          aTCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑TAAAAAAAAATTAATATTTTTAAAAAACAAAAATATAAAAAAAAAATTAAAAAAAAaagttttt                                 >  2:3443985/1‑126 (MQ=255)
                                                                                           tCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAGATTTTGAAAAACAAAAAAAAAAACAAAAAAATAAAAAAAAAtaatttattata                             >  2:2174821/1‑123 (MQ=255)
                                                                                            cAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATATTTTGGAAAACCAAAAATAATATAAAAAAAAATaaaaaataatttatgttt                               >  2:1282279/1‑116 (MQ=255)
                                                                                            cAACATTACAACGCTATCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTTTTA‑‑‑AAAAAAAAAAATAAGTTTTGGAAAAAACAAACCTAAAAAAAAAAAATTAAAAAAAAAATTTTTTTTGa                             >  1:852184/1‑128 (MQ=255)
                                                                                              aCATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAAGTAATATTTGGATAAACCAAAAATATATAAAAAAAATTAAAAAAAAtatttttatttt                             >  1:545467/1‑122 (MQ=255)
                                                                                               cATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAAATTTTTGATAAACCAAAAATATAAAAAAAAAATTAAAAAAAAaatttttttttatt                           >  2:877708/1‑120 (MQ=255)
                                                                                               cATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAAGTAAGTTTTGGAACAACCAAAAATATAAAAAAAAAATTAAAAAAAAAAGTTTTTTTGAGtt                          >  2:3174773/1‑128 (MQ=255)
                                                                                                 ttACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAAATAAGATTTTGTAAACACAAAAAAATATAAAAAAAATAAAAAAAAAAAATTTTGTATAGtt                          >  2:2292288/1‑128 (MQ=255)
                                                                                                  tACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTATATTTTGGTTAAACAAAAATAATAAAAAAAAAATAAAAAAAAAtatttatttttatttta                        >  2:1889813/1‑116 (MQ=255)
                                                                                                   aCAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATATTTTGATAAACCAAAAATATATAAAAAAAAATAAAAAAAAtaatttattttatttaa                        >  1:2792067/1‑121 (MQ=255)
                                                                                                   aCAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAATTTTTTTTTACACCAAAAATAAATAAAAAAAATTAAAAAAAAAATTTTAATTTAtttta                        >  1:3915396/1‑127 (MQ=255)
                                                                                                   aCAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAAATTTTTAAAAAACAAAAAAATAAAAAAAAAATTAAAAAAAAtattttatttaatattaa                       >  2:1521930/1‑120 (MQ=255)
                                                                                                    cAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAAATTTTTAAAAACAAAAAATATAAAAAAAAAATTAAAAAAAATAATTTATTTTATTTTaaa                      >  1:1005737/1‑128 (MQ=255)
                                                                                                    cAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAAGTAAAATTTGTATCAACCAAAAAAAAATAAAAAAATTTAAAAAAAATAGTTTTTTTGAGTTTaaaa                     >  2:3884627/1‑128 (MQ=255)
                                                                                                      aCGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTTT‑‑‑TTTTTTTTTTTAT‑‑‑‑‑‑AAAAAAAAAAATAATATTTGGTAAAAACAAAAAATTAAAAAAAAAATTAAAAAAAATATTTTATTTTAATTTaaa                      >  1:172537/1‑128 (MQ=255)
                                                                                                      aCGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAATAAAATTTGGTTCAACCAAAAATTTAAAAAAAAAATTAAAAAAAATATTTTATTTTAGGTTCaaa                     >  1:1153118/1‑128 (MQ=255)
                                                                                                      aCGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAAATATTATTTGTTAAAAACAAAAATTTATAAAAAAAATTAAAAAAAAaattttattttattaaaaaag                    >  1:385493/1‑117 (MQ=255)
                                                                                                      aCGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAATTAAGATTTGGATCAACCAAAAATATATAAAAAAAATAAAAAAAAATAGTTTTTTTTAGTTTaaaaaaa                  >  1:995/1‑125 (MQ=255)
                                                                                                       cgatttCAATGTTTATTTTTTTTGA‑‑‑TT‑‑‑‑‑‑‑‑GT‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGc                <  2:3570466/125‑1 (MQ=255)
                                                                                                        gCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAATATTTGGATAAACAAAAAAAATTTAAAAAAAATAAAAAAAAATATTTTATTTgttttaaaaaa                    >  1:145813/1‑118 (MQ=255)
                                                                                                        gCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAAATTTTGTAAAACCAAAAATATATAAAAAAAATAAAAAAAAATATTTTATTTTATTTTaaaaaa                   >  1:2899952/1‑126 (MQ=255)
                                                                                                         cTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAAGAAATATTTTGATAAACACAAACTAAAAAAAAAAAAAAAAAAAAAAAAATTTAATTTAgataaacaaag                  >  1:3215965/1‑118 (MQ=255)
                                                                                                         cTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATTTTTTTTTAAAACAAAAAAAAATAAAAAAAATAAAAAAAAAAATTTTTTTTGTTTTTAAAAtaag                 >  2:3802930/1‑125 (MQ=255)
                                                                                                         cTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAAAAAAATTAATTTTTTGAAAACCCAAAAATAATAAAAAAAAATTAAAAAAAAAATTTTATTTGTTTTAAAAATCtt                 >  2:3108460/1‑127 (MQ=255)
                                                                                                            tCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTTTTTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAATATTTGTATAAACACAAACTAATACAAAAAAATTAAAAAAAAAAGTATAATTGAATTTCaaaatagca               >  2:1050555/1‑122 (MQ=255)
                                                                                                              aaaGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑TAAAATAAATTTAATATTTTGAAACAACAAAAAAATATAAAATAAATAAAAAAAAAATTTTTTGTTTAGTTTaaaaatataaaatat          >  2:2595140/1‑117 (MQ=255)
                                                                                                               aaGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTT‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAAATTTGGATCAACCAAAAATATATAAAAAAAATTAAAAAAAAATATTTATTTTATTTTAAAAACTTCAtatata         >  2:3603006/1‑128 (MQ=255)
                                                                                                                aGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAATTTTGGATAAACCAAAAATATTTAAAAAAAATTAAAAAAAATATTTTATTTTATTTTAAAATATGaataaata         >  1:1367559/1‑128 (MQ=255)
                                                                                                                  tgtATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTT‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑TAAAATAAAAATTAAAATTTGTAAAAACCAAAAAATTTAAAAAAAAATAAAAAAAAAAATTTTATTTGTTTTTAAAAtaggaaaatatat        >  2:3026516/1‑116 (MQ=255)
                                                                                                                   gtATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑GTTTT‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑TAAAATAAAAGTAAATTTTGGTTAAACCAAAAAATATTAAAAAAAATTTAAAAAAATATTTTATTTTATGTTAAAATCTGCAtatatatt       >  1:567594/1‑126 (MQ=255)
                                                                                                                   gtATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTTT‑‑‑‑‑‑AAAAATAAAATTAAGATTTTGATAAACAAAAAATATATAAAAAAAATTAAAAAAAATTTTTAATTTTATTTAAAAATCTACATTTATAGTaaaa   >  1:493861/1‑128 (MQ=255)
                                                                                                                                              |                                                                                                                                    
TTTTAATTTTTTTTATAAAAAAAAATAAAAATTTTTCATTTAAAGCCATTTGTAAAAAAGCCTATGTTCTAGGCTTTTTATTATACTTATCAACATTACAACGCTTTCAAAGTGTATTTTTTAGGC‑‑‑TT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑TTTTT‑‑‑TTTTTTTTTTTGT‑‑‑‑‑‑AAAAATAAAAGTAAGATTTGGATCAACCAAAACTATATCAAAAAAATTAAAAAAAATAGTTTAGTTGAGTTTCAAATCTGCATATATAGTACAAA  >  CP016816/203767‑204008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: