Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,023 2 bp→TA intergenic (‑744/‑392) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,0230AT99.5% 6482.5 / NA 2171intergenic (‑744/‑393)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (7/3);  new base T (1187/972);  total (1195/976)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP01681620,0240TA99.8% 6555.1 / NA 2170intergenic (‑745/‑392)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base A (1191/973);  total (1194/976)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2209 total aligned reads displayed.

GAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAGAT  >  CP016816/19899‑20147
                                                                                                                            ||                                                                                                                           
  gCAGGAAGAGCAAGATAAAAGGGAGGATTTGTTGGCGAGCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTC‑TTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                        <  2:452785/128‑1 (MQ=255)
   cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                        <  1:5030034/128‑1 (MQ=255)
    aGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtata                                                                                                                       >  1:4906017/1‑128 (MQ=255)
    aGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATATCTTTTTTTACTTTCTATTTTTAATTGATATATTtata                                                                                                                       >  1:4487202/1‑128 (MQ=255)
     tgaattgCAAGATAATACGTAGTATTTGTTGGCGATACCCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                      <  2:1039442/122‑1 (MQ=255)
      gAAGAGCAAGATAAAAGGTAGTATTTGTTTGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATATCTTTTATTACTTACTATTTTTAATTTAtatatttatatt                                                                                                                     >  1:4080105/1‑128 (MQ=255)
      gAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTTATTTAtatatttatatt                                                                                                                     >  2:5256911/1‑128 (MQ=255)
      gAAGAGCAAGATAAAAGGTAGTATTTGTTGGAGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTAtatatttatatt                                                                                                                     <  1:2852749/128‑1 (MQ=255)
       aaGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTa                                                                                                                    <  1:321416/128‑1 (MQ=255)
         gatcAAGATAAAAGGTAGTATTTGTTGGCGATTCACCTAGAGTCTTTTACATCATCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaa                                                                                                                  <  1:3874263/125‑1 (MQ=255)
         gagctcgattctaGGTAGTTTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGATAAGAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTTTTTTTAATTTATATATTTATATTaaa                                                                                                                  <  2:3603726/116‑1 (MQ=255)
          agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTAACTTTCTATTTTTCATTTATATATTTATATTaaaa                                                                                                                 >  1:1380309/1‑128 (MQ=255)
               gATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAAAAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                            <  1:3636775/128‑1 (MQ=255)
                aTAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTa                                                                                                           <  2:1898335/128‑1 (MQ=255)
                 tAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCATCGGAAAACAAAAACTATTTATTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                          <  2:684886/128‑1 (MQ=255)
                  aaaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                         <  2:5355373/128‑1 (MQ=255)
                     aGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatta                                                                                                      <  2:4247212/128‑1 (MQ=255)
                         agtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaa                                                                                                                   <  1:1495564/111‑1 (MQ=255)
                          gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                                 <  1:5389678/128‑1 (MQ=255)
                           taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTtaattataatta                                                                                                >  1:1856859/1‑128 (MQ=255)
                           taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                                >  1:4242774/1‑128 (MQ=255)
                           taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                                >  1:4363252/1‑128 (MQ=255)
                               tgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAtttt                                                                                            <  2:4461180/128‑1 (MQ=255)
                                gttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttttt                                                                                           >  2:3303415/1‑128 (MQ=255)
                                    gCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                       >  1:3311834/1‑128 (MQ=255)
                                    gCGATCCCACTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAg                                                                                       <  2:752584/128‑1 (MQ=255)
                                     cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAga                                                                                      >  2:310350/1‑127 (MQ=255)
                                     cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                      <  1:3729386/128‑1 (MQ=255)
                                     cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                      >  1:89027/1‑128 (MQ=255)
                                     cGATCCCACTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                      <  1:2035970/128‑1 (MQ=255)
                                      gATCCGCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                     <  2:1246218/128‑1 (MQ=255)
                                      gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                     <  1:1774808/128‑1 (MQ=255)
                                      gATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                     >  1:2959648/1‑128 (MQ=255)
                                         cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTTATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGt                                                                                  >  1:701144/1‑128 (MQ=255)
                                         cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTAATAGCACGt                                                                                  >  1:4811124/1‑128 (MQ=255)
                                         cccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTGTCTTTAATTTCATTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCAAGt                                                                                  >  2:4964425/1‑128 (MQ=255)
                                          ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                 <  2:54654/128‑1 (MQ=255)
                                          caccTAGAGTCTTTTACATCATCCGAAAACAAAAACTATTTTTTCTTTAATTTCTTTATTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                 <  2:1712106/126‑1 (MQ=255)
                                           cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATATTTATAGCACGTGa                                                                                >  2:1792085/1‑128 (MQ=255)
                                            catAGAGTCTTTTAGATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                               <  1:5527890/126‑1 (MQ=255)
                                            acTAGAGTCTTTTACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTATATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                               <  2:1479927/127‑1 (MQ=255)
                                               agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTTTAATTATTTTTATAGCACGTGATAAt                                                                            >  2:600006/1‑128 (MQ=255)
                                               agagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCa                                                                                     >  2:2551742/1‑119 (MQ=255)
                                                ttctCTTTTAAATCTTCGGAAAACAAAAACAATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAtt                                                                           <  2:5539662/125‑1 (MQ=255)
                                                  gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                         >  2:4598611/1‑128 (MQ=255)
                                                  gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCAAGTGATAATaaa                                                                         >  2:3496489/1‑128 (MQ=255)
                                                   tCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaa                                                                        >  1:517906/1‑128 (MQ=255)
                                                    cTTTTACATCTTCGGAAAACAAAAACTATATTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGttataattataa                                                                       >  1:2349318/1‑128 (MQ=255)
                                                     ttttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAg                                                                      >  2:1542937/1‑128 (MQ=255)
                                                       ttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGtt                                                                    >  2:4378986/1‑128 (MQ=255)
                                                         aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTATTATAGCACGTGATAATTAAAAGTTAg                                                                  >  2:138861/1‑128 (MQ=255)
                                                          cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGt                                                                 >  1:10932/1‑128 (MQ=255)
                                                            tCTTCGGAAAACACAAACTATTGATTCTATAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTAAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                               <  2:2481304/128‑1 (MQ=255)
                                                            tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                               <  2:2981616/128‑1 (MQ=255)
                                                            tCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                               <  2:4154662/128‑1 (MQ=255)
                                                              ttCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGttcac                                                             >  2:2734090/1‑124 (MQ=255)
                                                                 ggAAAAC‑AAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGa                                                         >  2:2284240/1‑128 (MQ=255)
                                                                   aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                        >  2:1620167/1‑128 (MQ=255)
                                                                     aaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAATTTAGTGAACAAGaaaa                                                      >  1:599629/1‑128 (MQ=255)
                                                                     aaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaaa                                                      >  2:1317864/1‑128 (MQ=255)
                                                                      aCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAAc                                                     >  2:3199356/1‑128 (MQ=255)
                                                                       cAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGTACAAGAAAACa                                                    <  2:4088267/128‑1 (MQ=255)
                                                                       cAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACa                                                    >  2:2853319/1‑128 (MQ=255)
                                                                           aaCTATTTTTTCTTTAATTT‑ATTTTTTACTTTATATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTATTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                               <  2:2127595/128‑1 (MQ=255)
                                                                             cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                              <  1:2148244/128‑1 (MQ=255)
                                                                               aatttttCTTTAATTTCTTTTTTTACTTTCAATATTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGATAATGATAATTAAAAATTAGTGAAAAAGAAAACAGTCaataa                                            >  1:526724/3‑125 (MQ=255)
                                                                               aTTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCa                                            <  2:3703881/128‑1 (MQ=255)
                                                                                ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACCGTGAAGCAc                                           >  1:3530732/1‑128 (MQ=255)
                                                                                ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                           >  2:4762670/1‑128 (MQ=255)
                                                                                ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                           >  2:2218285/1‑128 (MQ=255)
                                                                                  ttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCa                                         <  1:4589750/128‑1 (MQ=255)
                                                                                    ttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGt                                       >  1:3442041/1‑128 (MQ=255)
                                                                                      cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                     >  1:960393/1‑128 (MQ=255)
                                                                                      cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                     <  2:4013989/128‑1 (MQ=255)
                                                                                      cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                     >  2:2414398/1‑128 (MQ=255)
                                                                                      cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTCATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                     >  2:3843926/1‑128 (MQ=255)
                                                                                      cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTAATAGCACGTGATAATTAAAAGTTAGTGAACAATAAAACATTGAAACACAAGttt                                     >  1:3136805/1‑128 (MQ=255)
                                                                                      cTTTAATTTCTATTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAATCACCAGttt                                     >  2:5075979/1‑128 (MQ=255)
                                                                                      atttAAATTCATTATTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTAAAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                     <  1:1637459/127‑1 (MQ=255)
                                                                                        ttAATATCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCt                                   <  2:1131697/128‑1 (MQ=255)
                                                                                                    tttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTCAAAAAGAAAACAGTGAAGAAACAGTTTCTGAACCAAaagaa                       <  1:79256/128‑1 (MQ=255)
                                                                                                     ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                      >  2:863092/1‑128 (MQ=255)
                                                                                                     ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaag                      >  1:2498583/1‑128 (MQ=255)
                                                                                                     ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACACTGAAGCACCAGTTTCTCAACCAAaagaag                      >  2:5176713/1‑128 (MQ=255)
                                                                                                      tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagaaga                     <  1:2639945/128‑1 (MQ=255)
                                                                                                       aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                    <  1:1961750/128‑1 (MQ=255)
                                                                                                       aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGAc                    >  2:2633806/1‑128 (MQ=255)
                                                                                                        cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                   >  2:4669395/1‑128 (MQ=255)
                                                                                                        cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                   <  1:4691466/128‑1 (MQ=255)
                                                                                                          ttCTATTTTTAATTTATATAATTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa                 <  1:1301538/128‑1 (MQ=255)
                                                                                                           tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa                <  2:3915647/128‑1 (MQ=255)
                                                                                                           tCTATTTTTAATTTATATATTTATATTAAAAAATTAAAATTATAATTATTTTTATAGCACGTGATATTTAAAAGTAAGTGAACAATAAAACAGTGAAGCACCAGTTTCTGAACAAAaataattctaaa                >  1:1004013/1‑121 (MQ=255)
                                                                                                            cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACATTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa               >  1:2768600/1‑128 (MQ=255)
                                                                                                            cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa               >  2:2024059/1‑128 (MQ=255)
                                                                                                            cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa               >  1:39684/1‑128 (MQ=255)
                                                                                                            cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa               >  2:4866992/1‑128 (MQ=255)
                                                                                                             tATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaa              >  1:4158587/1‑128 (MQ=255)
                                                                                                              aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa             >  2:3377055/1‑128 (MQ=255)
                                                                                                               tttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAAc            >  1:2249691/1‑128 (MQ=255)
                                                                                                                ttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGATGACGAAAAAACa           >  1:1220969/1‑128 (MQ=255)
                                                                                                                            ||                                                                                                                           
GAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAGAT  >  CP016816/19899‑20147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑