BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	281,616	C→T	Q100* (CAA→TAA)	JCVSYN2_01185 →	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	281,616	0	C	T	99.9%	8440.5 / NA	2634	Q100* (CAA→TAA)	JCVSYN2_01185	DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (1290/1339); minor base G (2/0); total (1293/1341)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2653 total aligned reads displayed.

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

  gATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAtt                                                                                                                           <  2:1929883/128‑1 (MQ=255)

  gATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAtt                                                                                                                           <  2:3724309/128‑1 (MQ=255)

  gATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAtt                                                                                                                           <  1:4563058/128‑1 (MQ=255)

  gATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAtt                                                                                                                           <  1:1978866/128‑1 (MQ=255)

          ttCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaaaaa                                                                                                                   <  1:3007817/128‑1 (MQ=255)

           tCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAg                                                                                                                  >  2:5208007/1‑128 (MQ=255)

            cTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGt                                                                                                                 <  2:3370076/128‑1 (MQ=255)

             tAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGtt                                                                                                                <  1:3669277/128‑1 (MQ=255)

             tAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGtt                                                                                                                <  1:1877254/128‑1 (MQ=255)

              aaaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGttt                                                                                                               <  2:224374/128‑1 (MQ=255)

               aaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAATTTTa                                                                                                              >  1:4356611/1‑128 (MQ=255)

                aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTATAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaa                                                                                                             >  2:1221740/1‑128 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                            >  2:2714952/1‑128 (MQ=255)

                  cTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaaa                                                                                                           >  1:4950987/1‑128 (MQ=255)

                    aGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAaga                                                                                                         >  2:4106117/1‑128 (MQ=255)

                    aGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAaga                                                                                                         >  2:1399774/1‑128 (MQ=255)

                     gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                        <  1:1679492/128‑1 (MQ=255)

                         attattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGa                                                                                                    <  2:5016956/128‑1 (MQ=255)

                          ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                   <  1:136511/128‑1 (MQ=255)

                          ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                   >  1:4795678/1‑128 (MQ=255)

                          ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGAAGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                   >  1:2924992/1‑128 (MQ=255)

                           tattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAtt                                                                                                  >  2:380744/1‑128 (MQ=255)

                            attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                 <  2:2819718/128‑1 (MQ=255)

                            attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                 <  1:628057/128‑1 (MQ=255)

                              tattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAAGCAAATAGATGAAAAATAAGTTATAAAAAGTTTAAAAGAGGTGATTtga                                                                                               >  2:392666/1‑128 (MQ=255)

                                tttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgatg                                                                                             <  1:541529/128‑1 (MQ=255)

                                 ttaatttaGAGACAAAATTACATTTAACACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACATATCAAATAGATGAAAAATATTTTATAAAAAGTTTAAAAGAGGGGATTtgatga                                                                                            <  1:4143340/128‑1 (MQ=255)

                                 ttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgatga                                                                                            >  2:1837803/1‑128 (MQ=255)

                                  taatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGaa                                                                                           >  1:2126070/1‑128 (MQ=255)

                                   aatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGaaa                                                                                          <  1:2448705/128‑1 (MQ=255)

                                   aatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGaaa                                                                                          <  1:2320894/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                         <  1:3830653/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGAGTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                         <  1:3408918/128‑1 (MQ=255)

                                     tttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGt                                                                                        <  1:5312824/128‑1 (MQ=255)

                                      ttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGATAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTTATGAAAGTa                                                                                       >  2:4812374/1‑128 (MQ=255)

                                       taGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAt                                                                                      <  2:642471/128‑1 (MQ=255)

                                       taGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAt                                                                                      <  2:890204/128‑1 (MQ=255)

                                        agagACAAAATTACATTTAAAACAAGAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATTAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAtt                                                                                     >  2:4481075/1‑128 (MQ=255)

                                           gaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAt                                                                                  <  2:2030900/128‑1 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GAt                                                                              <  2:4750547/128‑1 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GAt                                                                              >  1:856374/1‑128 (MQ=255)

                                               aaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAt                                                                                      <  2:4184098/120‑1 (MQ=255)

                                               aaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATc                                                                             <  2:290627/128‑1 (MQ=255)

                                                 aTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGAAGAAAAATAATTTATAAAAAGTTTAAAATAGTTGATTTGATTAAATTATTAAT‑CATCgt                                                                           >  2:1651973/1‑127 (MQ=255)

                                                   tACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGgag                                                                         <  2:426564/128‑1 (MQ=255)

                                                   tACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGgag                                                                         >  1:1785373/1‑128 (MQ=255)

                                                   tACATTAAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGgag                                                                         <  2:2415653/128‑1 (MQ=255)

                                                       tttAAACCAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAACCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGCTTTGATGAAAGTATTAAT‑GATCGGAGATGt                                                                     <  1:2746683/128‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTAAAAAGAGGTGATTTGATGAAAGTATTACT‑GAGCGGATATGttaa                                                                  >  2:492508/1‑126 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGa                                                                                                    >  1:5105947/1‑89 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCaaa                                                            <  1:552390/128‑1 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCaaa                                                            >  2:2524640/1‑128 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCaaa                                                            >  1:74583/1‑128 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCaaa                                                            >  1:3934495/1‑128 (MQ=255)

                                                                 aaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCaaaa                                                           <  1:4252964/128‑1 (MQ=255)

                                                                  aacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAAc                                                          >  2:4658611/1‑128 (MQ=255)

                                                                   acaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAAcc                                                         <  2:3151414/128‑1 (MQ=255)

                                                                    caGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCg                                                        >  2:1554324/1‑128 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCTGAGATGGTTATGaaaatcagg                                                       >  1:2637846/1‑123 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCgg                                                       <  1:1193292/128‑1 (MQ=255)

                                                                      ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCggg                                                      <  1:1000955/128‑1 (MQ=255)

                                                                        aTTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGaa                                                    >  2:4372959/1‑128 (MQ=255)

                                                                           tACTAATATCTCTAATTAAAAAAATTGATACAAATCATATAGATTAATAATAAATTATAAAAAGTTTAAAAGAGGTGATTAGATGATAGTAAAAAT‑GATCGCAGATGTTTATGCAAAACCGGTAagag                                                 >  1:2849140/1‑128 (MQ=255)

                                                                            aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCTGAGATGTTTATGCAAAACCGGGAAgaga                                                >  1:4668797/1‑128 (MQ=255)

                                                                            aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAgaga                                                <  1:159309/128‑1 (MQ=255)

                                                                            aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAgaga                                                <  1:5201954/128‑1 (MQ=255)

                                                                            aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGGGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAgaga                                                <  1:1091216/128‑1 (MQ=255)

                                                                             cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGaa                                               <  1:2533505/128‑1 (MQ=255)

                                                                               aaTTTCTCTAATAAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAAt                                             <  2:5096324/128‑1 (MQ=255)

                                                                                aTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCTAAACCGGGAAGAGAAATg                                            >  2:1312041/1‑128 (MQ=255)

                                                                                     tctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGa                                       <  1:4036064/128‑1 (MQ=255)

                                                                                         attaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaaa                                   <  1:1433349/128‑1 (MQ=255)

                                                                                         attaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaaa                                   <  1:4452008/128‑1 (MQ=255)

                                                                                          ttaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAAc                                  >  2:4578067/1‑128 (MQ=255)

                                                                                           taataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACa                                 <  1:222019/128‑1 (MQ=255)

                                                                                           taataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACa                                 >  2:5534860/1‑128 (MQ=255)

                                                                                             aaaaaaTTTAAACAAAACCAATAAATGAAAAATTATTTATAAAAAATTTTAAATAGGGTATTTGATGAAAATATTTATGGAAGGGACTTG‑TTATGCAAAAACGGGAATAGAAATGCTAGAAAAACaat                               >  2:2994348/3‑126 (MQ=255)

                                                                                              taaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAACCAttt                              >  2:5402907/1‑128 (MQ=255)

                                                                                               aaaaTTGAAACAAATCAACTAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAGTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTa                             <  1:3544626/128‑1 (MQ=255)

                                                                                                 aaTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTata                           >  1:3127412/1‑126 (MQ=255)

                                                                                                   ttGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTGTGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaaaa                         >  1:333840/1‑128 (MQ=255)

                                                                                                      aaaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAata                      <  2:2604201/128‑1 (MQ=255)

                                                                                                        aCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAtt                    <  2:809131/128‑1 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCTGGAAGAGAAATGCTAGAAAAACATTTATAAAATAttgt                  >  2:4004627/1‑128 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAGGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  <  2:747904/128‑1 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  <  1:945164/128‑1 (MQ=255)

                                                                                                           aatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgtt                 >  2:1485693/1‑128 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGATCAACATTTAAAAAATAttgttg                >  2:3095647/1‑128 (MQ=255)

                                                                                                               aaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatc             >  2:35211/1‑128 (MQ=255)

                                                                                                                 atAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaa           >  1:3214451/1‑128 (MQ=255)

                                                                                                                 atAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGAATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAATTGCTAGAAAAACATTTAAAAAATATTGTTGatcat           >  2:2133580/1‑127 (MQ=255)

                                                                                                                    taTGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGGTTTAAT‑GATCTGTGATTTTTATGCAAAAACTTGAAGAAAAATGATAGAAAAACATTTAATAAATATTGTGTatcaaaat        >  2:2345254/2‑128 (MQ=255)

                                                                                                                     aTGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatc       <  1:5038783/128‑1 (MQ=255)

                                                                                                                     aTGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTGGatcaaaatc       >  2:1019246/1‑128 (MQ=255)

                                                                                                                       gAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATAGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaa     <  2:3246894/128‑1 (MQ=255)

                                                                                                                        aaaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  2:5301691/128‑1 (MQ=255)

                                                                                                                        aaaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  2:1130122/128‑1 (MQ=255)

                                                                                                                        aaaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  2:1719437/128‑1 (MQ=255)

                                                                                                                        aaaaaTAATTTATAAAAAGGTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatcaaa    <  1:3768086/128‑1 (MQ=255)

                                                                                                                          aaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCATAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATa  <  1:1260524/128‑1 (MQ=255)

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAT‑GATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`