Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA99.0% 6386.1 / 37.7 2092intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (1281/777);  minor base . (4/17);  total (1289/803)
Fisher's exact test for biased strand distribution p-value = 1.11e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2142 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATA  >  CP016816/165097‑165350
                                                                                                                               |                                                                                                                                
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                >  2:1419152/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               <  2:3791183/128‑1 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               <  2:100170/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  2:5500674/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:5325911/128‑1 (MQ=255)
       tGTATTAATTTTCTAGTTGATATTATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAATACTATTTTCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  2:4399974/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                        <  1:2999830/128‑1 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTaa                                                                                                                      >  1:2476183/1‑128 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      >  1:4513439/1‑128 (MQ=255)
              ctttactAGTTGATAATATTTAGTTTCATTATTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                                  <  2:687686/128‑1 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCTCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 <  2:3311582/128‑1 (MQ=255)
                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTa                                                                                                                >  1:726450/1‑128 (MQ=255)
                 tactAGTTGATACTATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               <  1:2819487/128‑1 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               >  1:2645605/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             <  2:2169759/128‑1 (MQ=255)
                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                            >  1:3164262/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                           >  1:4950078/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGTAAAAAATAGc                                                                                                       >  2:594992/1‑128 (MQ=255)
                          ataataTTTAGTTTCATTAATTTATAGCACTTGTATGAATTAGTAACAAACTAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCt                                                                                                      <  1:1169780/128‑1 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                    >  2:271285/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttt                                                                                                   >  1:2554399/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                   >  1:3268356/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttt                                                                                            >  1:2167846/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAATATAGCtatttttttttt                                                                                           >  2:1099648/1‑128 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                       >  1:5028424/1‑128 (MQ=255)
                                          ttAATTTATCTCAATTTTTTGAATTAGTAATAAAATAAAAATATTATTTTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGCtttttttttttttttat                                                                                      <  1:4669597/128‑3 (MQ=255)
                                          ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATAtttttttttttttttaa                                                                                      >  1:3445729/1‑126 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTatat                                                                                  >  2:2561365/1‑128 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtaaatttaaaaa                                                                         >  2:2375188/1‑118 (MQ=255)
                                                        ttGTATGTATTATTAAAAAAAATAAAATATTTTATTTTTTTATATTTTTTTTTTTTTAAAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                      <  2:3432683/128‑1 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAAaata                                                                      >  2:3224251/1‑128 (MQ=255)
                                                         tGTATTAATTAGTAATAAAATAAAAATATTATATTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                     <  1:1010645/128‑1 (MQ=255)
                                                          ttttttaTTAGTAATAAAATAAAAATATTTTATTTTTTTAATATTTTTGTTAATTCAATTTTTTAAAAAATTTATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                    <  2:5261801/122‑1 (MQ=255)
                                                          gTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAt                                                                   <  1:458893/128‑1 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAAttt                                                                 >  1:1619416/1‑128 (MQ=255)
                                                               taTTAGTTATAAAATAAAAATATTATTTTTTTTTTTATTTTTTTTTTTTTTAAATTTTTTAAAAATTAATTTGAAATTTTTAAAAATTGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                               <  1:3953127/127‑1 (MQ=255)
                                                                aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTat                                                              >  1:5082404/1‑127 (MQ=255)
                                                                aTTAGTAAGAAACTAAAACCATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAAATATAAAtttt                                                                >  1:1813809/1‑128 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAATAAATAGCTTTTTTTTTTTTTTTTTATTATTTGAATTTTAAAtttttat                                                             >  2:4157449/1‑125 (MQ=255)
                                                                   attaataaACTAAAAAAATATTATTTTTATTTATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAAAACC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                           <  2:3117510/126‑1 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTatttattt                                                        >  1:4426835/1‑128 (MQ=255)
                                                                     taataaACTAAAAATATTATTTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                        <  1:827593/128‑1 (MQ=255)
                                                                       atatattaaaactaTTTTATTATTAGATAATTTTTTTATTTTTAAAATTTTAAAAAATTTATTTGTTCTTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtttt                                                       <  1:3570153/122‑1 (MQ=255)
                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTAttttt                                                      >  1:1430376/1‑127 (MQ=255)
                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTAGTTAtttttt                                                     >  1:3076375/1‑126 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTAGTTAtttttt                                                     >  1:124922/1‑126 (MQ=255)
                                                                         aaaataaaaattTTTTTTTTTTTTATATTTTTTTTAAATGCAAAATTTTAAAAAATTAATTTTTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                    <  2:4490582/128‑1 (MQ=255)
                                                                         aaaataaaaataTTATATTTTTTTTTATTTTTTTTTTTTTTAAATTTTTAAAAAATTTATTTTAAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                      <  2:1906356/128‑1 (MQ=255)
                                                                            ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATATAATATAAATAAAAATTTAAATTATtttttta                                                   >  1:556399/1‑127 (MQ=255)
                                                                            ataaaaataTTTTGTTTTTTTTTATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                  <  1:2347288/127‑1 (MQ=255)
                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTAAATGTAAATTTAAATTTTTGGTATTTTTTTTTCt                                                >  2:3529841/1‑128 (MQ=255)
                                                                               aaactaTTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                              <  1:2711666/128‑1 (MQ=255)
                                                                                  cttttttCTTTTTTTATATTTTTTGTATATGTAAATTTTTAAAAAATTTTTTTTAACTTTGAAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGt                                            <  1:1552895/122‑1 (MQ=255)
                                                                                   tattatTTTTTATTATATTTTTTTTATTTGTAAATTTTTAAAAAATTAATTTGAACTTTTTAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                          <  1:3751793/128‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAGTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTTAATATTAATTTTAGTTATTTttttgtaatatttt                                          >  2:1628222/1‑120 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑‑‑ATGGAAATATAAATTTTATTTAATTttttgtaatagtat                                          >  2:5113915/1‑120 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTATAATTa                                           >  1:1980185/1‑128 (MQ=255)
                                                                                    attttttttttATATATTTTTTTTTTATTAAAATTTTTAAAAAAATAAATTTAAATTTTTAAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                           <  1:2089694/122‑1 (MQ=255)
                                                                                    attatCTTTTTTGATATATTTTGTTTATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                          <  1:4840434/128‑1 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATTTAAATATAAATTTTATTTATttttttttatattttt                                          >  2:4941586/1‑118 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGAAAATATAATTTTTAGGTATTTTTTTGACTAAGTAt                                          >  2:3653805/1‑128 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTAta                                         >  1:3366167/1‑127 (MQ=255)
                                                                                    attatAATTTTATAAATTTTTTTTATATTAAATTTTTTAAAAATTAAATTTTAATTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                          <  1:1914535/128‑1 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTAATATTTTAAATATAAATTTTAGGTATTtttttgaaaaatttt                                          >  2:4270037/1‑119 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAta                                         >  2:5026375/1‑127 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTAGTTATTTTGTTTTATAATTAta                                         >  1:5455703/1‑127 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTTTAAAAGTAta                                         >  1:5183105/1‑127 (MQ=255)
                                                                                      tttttatttATATATTTTTTGTTATATCAAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                         <  2:2845043/122‑1 (MQ=255)
                                                                                      tatTTTTTTAGATATTTTTTTTATATGAAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAAAAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                        <  1:4301946/128‑1 (MQ=255)
                                                                                          tttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTTTAATTAAAAATTTTAGTTATTTTTTTTTATAATTATATaaaaa                                   >  2:2069052/1‑128 (MQ=255)
                                                                                          tttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTATTTATTTTTTTGTATAATTATATaaaaa                                   >  2:2470445/1‑128 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATAAAAATATAAATATTATTAATTTTTATTTCTAATTATATaaaaa                                   >  1:2909998/1‑128 (MQ=255)
                                                                                            tttAGAAATTTTTTTTGAATTGAAATTTTTAAAAAATTAATTTTAATATTTAATAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaa                                  <  1:3647523/128‑1 (MQ=255)
                                                                                                tttatTTTTTTTTTATGAAAATTTTTTAAAAATTTATATTAATTTTTAAAAAAAA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTTTCTAAGTATCTAAAAAATTata                             <  2:537807/126‑3 (MQ=255)
                                                                                                tattttttttttttATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                             <  2:1218481/124‑1 (MQ=255)
                                                                                                tatatTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAATA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                             <  2:4587706/127‑1 (MQ=255)
                                                                                                gATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                          <  1:654953/128‑1 (MQ=255)
                                                                                                    ttttttGTTAATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                           <  1:4225598/128‑1 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTTTTTTGTTTTATAAGTATATAAAAAATTAGAAATGaaa                      >  2:1520931/1‑128 (MQ=255)
                                                                                                         ttttttttttaaaTTTTTTAAAAATTAATTTTTAATTTTATAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                      <  2:2289731/118‑1 (MQ=255)
                                                                                                         ttttataTTTAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                      <  2:816482/125‑1 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTATTTTTCTAAAAAAATATAAATGaaa                      >  1:4751913/1‑128 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATATTTTTCTAAAAAAATATAAATAaaaa                     >  1:4058867/1‑128 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAATAAAAAATTTTATTTATTTTTTTTTATAAATATAAAAAAAAAAATAAATAaaaa                     >  2:1962475/1‑128 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTTTTTTATAATTATATAAAAAAATATAAATTaaaaaa                   >  1:1332328/1‑128 (MQ=255)
                                                                                                          tttataTTCAAATTTTTAAAAAAATAATTTGTACTTTTAAAAAAAA‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa                    <  2:4716922/126‑1 (MQ=255)
                                                                                                             ttatttaaaTTTTTTAAAAATTAATTTTAAATTTTAAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaata                  <  1:1726003/122‑3 (MQ=255)
                                                                                                              ttttttaaTTTTTTAAAAATTAATTTTTATTTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAATGAAAAAatat                <  2:3913073/122‑1 (MQ=255)
                                                                                                              tttttaaaTTTTTAAAAAATTAATTTTTACTTTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                  <  1:109808/123‑1 (MQ=255)
                                                                                                              taagtaaaTTTTTAAAAAATTTATTTTAAATTTT‑‑AAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat                <  1:4141669/123‑1 (MQ=255)
                                                                                                                tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTAATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAata                 >  1:2268148/1‑128 (MQ=255)
                                                                                                                tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTGTATAAGTATATAAAAAATTATAAAGTAAAAAatat                >  1:382106/1‑128 (MQ=255)
                                                                                                                  cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTATTATAATGTAAAAATAAATTATAATAATTTTTTATTCTAAATAACTAAAAAAATAAAAATAAAAAAATAtttt             >  1:925665/1‑126 (MQ=255)
                                                                                                                   aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAAAAAAAATTTAATTTATTTTTTTGTATAATTAAAAAAAAAAATTAAAAAAAAAAAAAATTTTTa           >  2:904335/1‑128 (MQ=255)
                                                                                                                       ttttTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAg       <  2:1332328/128‑1 (MQ=255)
                                                                                                                        tttAAAAAATTAATTTGAAATTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa     <  2:2988408/128‑1 (MQ=255)
                                                                                                                          ttaaaaaaTTAATTTTTAAATGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGt      <  2:1791395/126‑1 (MQ=255)
                                                                                                                          ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTGTATAATTATATAAAAAAATATAAATTAAAAAATATTTTTaaaatt      >  1:5259258/3‑126 (MQ=255)
                                                                                                                           taaaaaaTTAATTTGAACATTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa     <  2:4824002/127‑1 (MQ=255)
                                                                                                                           aaaaaaTTTAATTTTAAATTTTAAAAATT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa     <  2:406255/128‑1 (MQ=255)
                                                                                                                               |                                                                                                                                
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATA  >  CP016816/165097‑165350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: