Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,795 A→T intergenic (‑104/+67) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7950AT99.2% 7799.5 / ‑2.9 2552intergenic (‑104/+67)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (17/3);  new base T (1243/1288);  total (1261/1291)
Fisher's exact test for biased strand distribution p-value = 1.27e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2606 total aligned reads displayed.

AAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTT  >  CP016816/484672‑484914
                                                                                                                            |                                                                                                                       
 aaaaaTAGTCAAAACTCATAGATTTAATCATTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                    <  1:4359985/128‑1 (MQ=255)
           aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                          <  1:3536126/128‑1 (MQ=255)
            aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  1:808152/1‑128 (MQ=255)
             aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaaac                                                                                                        >  1:2696425/1‑128 (MQ=255)
              aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTTACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAAATATAAAAAGCAATTAAacaca                                                                                                       >  1:4469131/1‑128 (MQ=255)
              aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                       >  1:2820128/1‑128 (MQ=255)
              aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                       >  1:920858/1‑128 (MQ=255)
              aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                       >  2:4616041/1‑128 (MQ=255)
                tCATAGATTTAATCCTTTTATATTTGACCAAAAAATAAGAGAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                     <  2:3822560/128‑1 (MQ=255)
                tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                     >  1:4635735/1‑128 (MQ=255)
                  aTAGATTTATTCCTTTTATAGTTGACCAAATAATTAGATAGATCATAGCAGAAGCTATTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                  <  2:4255287/128‑1 (MQ=255)
                   tAGATTTAATACTTTTATAGTTTACAAATAAATTAGATAGAGCATAGCATAATCTCTTAAAAAATAAACTACAATAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                  <  1:5015978/128‑1 (MQ=255)
                    aGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaaa                                                                                                 <  1:2468849/128‑1 (MQ=255)
                       tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAAATc                                                                                              >  1:2556364/1‑128 (MQ=255)
                        ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                             >  1:4915404/1‑128 (MQ=255)
                         tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                            >  2:194399/1‑128 (MQ=255)
                         tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACACGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCaa                                                                                            >  2:5172874/1‑127 (MQ=255)
                          aaTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTATTTTTTAATATAATAAGCAATTAAGAACACAAAAACACAcc                                                                                           >  2:278489/1‑128 (MQ=255)
                           aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                          <  1:3968872/128‑2 (MQ=255)
                           aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                          <  2:622387/128‑2 (MQ=255)
                             ccTTTTATAGTTGACCAAAAAATACGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                        <  1:3634128/128‑4 (MQ=255)
                               ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                      <  1:3869615/128‑1 (MQ=255)
                                tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTATACTACACTCGATTTTTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAAACCCCCAGTAt                                                                                     >  2:3613424/1‑128 (MQ=255)
                                tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATTTAAAAAGCAATTAAACACACAAAAACTCAcaaaatat                                                                                     >  1:5194590/1‑121 (MQ=255)
                                  tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                   <  1:1306633/128‑1 (MQ=255)
                                  tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                   <  2:5525019/128‑1 (MQ=255)
                                   ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                  <  1:3090959/128‑1 (MQ=255)
                                     aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTCACCCATTATGCTGa                                                                                >  1:2275933/1‑128 (MQ=255)
                                      gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAg                                                                               >  1:1613054/1‑128 (MQ=255)
                                       ttGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATGTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACAAAAAAACTCACCCATTAAGGTAAGt                                                                              >  2:5076811/1‑128 (MQ=255)
                                        tGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGt                                                                              <  2:4322155/128‑1 (MQ=255)
                                          gaccaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                           <  1:3355357/124‑1 (MQ=255)
                                          aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCTCCCATTATGGTGAGTTTa                                                                           <  1:5496955/128‑1 (MQ=255)
                                            cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                     <  1:4822504/100‑1 (MQ=255)
                                            cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaa                                                                         >  1:3741239/1‑128 (MQ=255)
                                             aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  1:1007664/128‑1 (MQ=255)
                                             aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  2:1307127/128‑1 (MQ=255)
                                             aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACCAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  1:1219105/128‑1 (MQ=255)
                                              aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                       <  1:198754/128‑1 (MQ=255)
                                               aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                      >  2:4975086/1‑128 (MQ=255)
                                                aaaTAAGATAGAGCATAGCAGAAGATCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTATAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                     <  1:1678993/128‑1 (MQ=255)
                                                 aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatta                                                                    >  1:3179831/1‑128 (MQ=255)
                                                        tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                             >  1:2049810/1‑128 (MQ=255)
                                                          gagCATAGCAGAAGCTCTTAACAACTGAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           >  1:5427499/1‑128 (MQ=255)
                                                          gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           >  2:2384713/1‑128 (MQ=255)
                                                          gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                           >  2:2149752/1‑128 (MQ=255)
                                                           agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaaat                                                          >  2:4512617/1‑126 (MQ=255)
                                                           agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                          >  1:455037/1‑128 (MQ=255)
                                                               tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                      <  1:4141117/128‑1 (MQ=255)
                                                               tAGCAGAAGCTCTTAACAACTAAACTACAATCGATTATTTAAATATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                      <  2:4915552/128‑1 (MQ=255)
                                                                 gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                    >  2:102171/1‑128 (MQ=255)
                                                                  attaaGGTCTTATCAACTAAACTATACTTGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattat                                                   <  2:2579897/125‑1 (MQ=255)
                                                                   aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                  <  1:5113592/128‑1 (MQ=255)
                                                                   aGAAGCTCTTAACAACTAAACTACAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                  <  2:4135391/128‑1 (MQ=255)
                                                                    gtaggtctTTACATCTAAAGTACACTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                 <  2:2285994/123‑1 (MQ=255)
                                                                    gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                 >  1:2906378/1‑128 (MQ=255)
                                                                      aGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTa                                               >  1:696167/1‑128 (MQ=255)
                                                                       gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                              <  2:1202641/128‑1 (MQ=255)
                                                                         tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATAATTTAAAAt                                            >  2:2472605/1‑128 (MQ=255)
                                                                           ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                           <  1:4227090/128‑1 (MQ=255)
                                                                             aacaacTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                        <  2:811951/128‑1 (MQ=255)
                                                                              acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                             >  1:1890154/1‑122 (MQ=255)
                                                                              acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                       >  2:93172/1‑128 (MQ=255)
                                                                                aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     <  1:62114/128‑1 (MQ=255)
                                                                                aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     >  1:3267733/1‑128 (MQ=255)
                                                                                aactaaactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAACCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     <  2:139209/128‑1 (MQ=255)
                                                                                  ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                   <  1:2157380/128‑1 (MQ=255)
                                                                                  ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATAATGTTGAGTTAAAAAATTATTCATAAATTTGATTATTTTAAATCAATTGAAg                                   >  2:4403815/1‑128 (MQ=255)
                                                                                       ctaCACTCGATTATTTAATTATATTTTTTTTTATTATAAAAAGCAATTAACCACAAAAAAACTCCCCCATTATGGTGAGTTTAAAATTTATACAAAAATTTAATTATTTTAAATCAATTGaaacacct                              >  2:1598649/1‑122 (MQ=255)
                                                                                       ctaCACTCGATTATTTAATTATATTTTTTTTTAATATACAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAATGATGATTATTTTAAATCAATAGATGCTCCa                              >  2:2887909/1‑128 (MQ=255)
                                                                                       ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                              >  1:4721815/1‑128 (MQ=255)
                                                                                        taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                             <  2:3154951/128‑1 (MQ=255)
                                                                                        taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                             >  2:298153/1‑128 (MQ=255)
                                                                                          cacTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                            <  2:5389658/128‑1 (MQ=255)
                                                                                           tctctATTATTTAATTAAATTTTTTTTTAATATAAAAAGCATTTAAACAATCAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                          <  2:1491149/127‑1 (MQ=255)
                                                                                           acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCATTTAAACACACAAAAACTACCCCATTATGGTGAGTTTAAAAATTATACAAAAATTTGATAATTTAAAAACAATTGAAGATCCacatt                          >  1:5311996/1‑124 (MQ=255)
                                                                                           acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGcat                          >  1:3007032/1‑126 (MQ=255)
                                                                                           acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATAATTTTAAATCAATTGAAGCTCCAGCtt                          >  2:3506930/1‑128 (MQ=255)
                                                                                            cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                         >  1:1521182/1‑128 (MQ=255)
                                                                                            cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                         >  1:1426884/1‑128 (MQ=255)
                                                                                             tCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                        <  2:3388501/128‑1 (MQ=255)
                                                                                              cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                       >  2:1845094/1‑128 (MQ=255)
                                                                                               gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                      >  2:4818142/1‑128 (MQ=255)
                                                                                               gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                      >  2:3067623/1‑128 (MQ=255)
                                                                                                attaatTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCTTTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                     <  2:1117588/128‑1 (MQ=255)
                                                                                                   attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                  <  2:4011980/128‑1 (MQ=255)
                                                                                                    tttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAtt                 <  2:4722856/128‑1 (MQ=255)
                                                                                                    tttAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                <  2:3269393/128‑1 (MQ=255)
                                                                                                       aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCACCTAATTTAg              >  2:722429/1‑128 (MQ=255)
                                                                                                       aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg              <  1:1111755/128‑1 (MQ=255)
                                                                                                         ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                           >  2:5243107/1‑113 (MQ=255)
                                                                                                         ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCTACTAATTTAGCt            >  1:2374908/1‑128 (MQ=255)
                                                                                                           atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt          >  2:1688415/1‑128 (MQ=255)
                                                                                                             atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATATAGCTTTCa        >  2:2680168/1‑128 (MQ=255)
                                                                                                              tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt       >  1:4048208/1‑128 (MQ=255)
                                                                                                              ttattttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt       <  2:2034113/125‑1 (MQ=255)
                                                                                                               ttttttttaATATAAAAAGAAATTAAACCCACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt      <  2:368384/128‑1 (MQ=255)
                                                                                                                tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt     <  2:3921720/128‑1 (MQ=255)
                                                                                                                 ttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt      >  1:3438348/1‑126 (MQ=255)
                                                                                                                  tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct   >  1:1794004/1‑128 (MQ=255)
                                                                                                                            |                                                                                                                       
AAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTT  >  CP016816/484672‑484914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: