Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,793 A→T intergenic (‑102/+69) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7930AT97.1% 7433.8 / 81.2 2552intergenic (‑102/+69)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (46/29);  new base T (1215/1262);  total (1261/1291)
Fisher's exact test for biased strand distribution p-value = 4.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 2609 total aligned reads displayed.

TATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTT  >  CP016816/484667‑484914
                                                                                                                              |                                                                                                                           
     aaaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTCCACTCGATTTTTTAATTATATTTTTTTTTTaatataaa                                                                                                                      >  2:4401548/1‑128 (MQ=255)
      aaaaaTAGTCAAAACTCATAGATTTAATCATTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                     <  1:4359985/128‑1 (MQ=255)
           tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATTAAAAGCAAt                                                                                                                >  2:1604262/1‑128 (MQ=255)
           tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                                >  2:3070244/1‑128 (MQ=255)
              tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAGTATTTAAATATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                             >  2:5383788/1‑128 (MQ=255)
                aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                           <  1:3536126/128‑1 (MQ=255)
                  aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                               >  2:1495968/1‑122 (MQ=255)
                   aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTTACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAAATATAAAAAGCAATTAAacaca                                                                                                        >  1:4469131/1‑128 (MQ=255)
                   aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                        >  1:920858/1‑128 (MQ=255)
                    cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                       >  2:3389925/1‑128 (MQ=255)
                     tCATAGATTTAATCCTTTTATATTTGACCAAAAAATAAGAGAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                      <  2:3822560/128‑1 (MQ=255)
                     tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                      >  1:4635735/1‑128 (MQ=255)
                       aTAGATTTATTCCTTTTATAGTTGACCAAATAATTAGATAGATCATAGCAGAAGCTATTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                   <  2:4255287/128‑1 (MQ=255)
                       aTAGATTTAATCATTTTATAGTTGAAAAAAAAATAAGATAGAGCATAGCAGAAGCTATTAACAACTAAACTACAATAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacacata                                                                                                    <  2:1846547/128‑3 (MQ=255)
                        tAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                   <  1:700317/128‑1 (MQ=255)
                        tAGATTTAATACTTTTATAGTTTACAAATAAATTAGATAGAGCATAGCATAATCTCTTAAAAAATAAACTACAATAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                   <  1:5015978/128‑1 (MQ=255)
                         aGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaaa                                                                                                  <  1:2468849/128‑1 (MQ=255)
                            tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAAATc                                                                                               >  1:2556364/1‑128 (MQ=255)
                             ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                              >  1:4915404/1‑128 (MQ=255)
                              tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                             >  2:3155876/1‑128 (MQ=255)
                              tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                             >  1:5499473/1‑128 (MQ=255)
                                ctCCTTTTATAGCAGACCAAAAAATAAGAGAGAGCATAGCAGCAGCTCTAAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                           <  1:2687711/127‑2 (MQ=255)
                                aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                           <  1:3968872/128‑2 (MQ=255)
                                  ccTTTTATAGTTGACCAAAAAATACGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                         <  1:3634128/128‑4 (MQ=255)
                                    ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                       <  2:3611898/128‑1 (MQ=255)
                                    ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                       <  1:3869615/128‑1 (MQ=255)
                                     tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATTTAAAAAGCAATTAAACACACAAAAACTCAcaaaatat                                                                                      >  1:5194590/1‑121 (MQ=255)
                                     tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAatat                                                                                      >  2:2682379/1‑128 (MQ=255)
                                     tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                      <  2:5171638/128‑1 (MQ=255)
                                     tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAt                                                                                      >  1:5428209/1‑128 (MQ=255)
                                       tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                             >  2:811751/1‑103 (MQ=255)
                                       tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                   <  1:1306633/128‑1 (MQ=255)
                                          aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTCACCCATTATGCTGa                                                                                >  1:2275933/1‑128 (MQ=255)
                                           gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAg                                                                               >  1:1772226/1‑128 (MQ=255)
                                             tGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATTATGGTGAGtt                                                                             >  2:718810/1‑128 (MQ=255)
                                               gaccaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                           <  1:3355357/124‑1 (MQ=255)
                                                 cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                      <  1:4822504/100‑1 (MQ=255)
                                                 cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaa                                                                         >  1:3741239/1‑128 (MQ=255)
                                                  aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  2:1307127/128‑1 (MQ=255)
                                                  aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  1:1007664/128‑1 (MQ=255)
                                                  aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACCAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                        <  1:1219105/128‑1 (MQ=255)
                                                   aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAATAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                       <  2:2984023/128‑1 (MQ=255)
                                                     aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                     <  1:1986391/128‑1 (MQ=255)
                                                     aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAatt                                                                     >  1:810491/1‑128 (MQ=255)
                                                      aaTAAGATAGATCATAGCAGAATCTCTTAACAACTAAAATACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCATAATGGGTGAGtttttgtgtgt                                                                    <  2:2386505/128‑9 (MQ=25)
                                                      aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatta                                                                    <  2:1952451/128‑1 (MQ=255)
                                                         aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                 >  2:3683090/1‑128 (MQ=255)
                                                           gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                               <  1:3085880/128‑1 (MQ=255)
                                                               gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTATTATAATATAAAAAGCAATTAAACACACAAAAACTCACCCATAATGCAGAGTTTAAAAAGTAATCAAACAt                                                           >  1:1678904/1‑128 (MQ=255)
                                                                agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                          >  1:455037/1‑128 (MQ=255)
                                                                    tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                      <  1:622186/128‑1 (MQ=255)
                                                                    tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                      <  1:4141117/128‑1 (MQ=255)
                                                                      gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                    >  2:102171/1‑128 (MQ=255)
                                                                        agaagaTATTAACAACTATACTACACTCTATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAATCTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                  <  2:2153588/128‑1 (MQ=255)
                                                                        aGAATCTCTTAATAACTAAAGTACACTCGATTATTTAATTATATTTTTTTTTAATATAATAAGCAATTAAACACACTAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatttaa                                                  <  2:4500507/128‑4 (MQ=255)
                                                                        aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                  <  1:5113592/128‑1 (MQ=255)
                                                                        aGAAGCTCTTAACAACTAAACTACAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                  <  2:4135391/128‑1 (MQ=255)
                                                                           aGCTCTTAACATCTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTGTATAAATTTTTCATAACTTTGATTATTTTa                                               >  2:5066394/1‑128 (MQ=255)
                                                                           aGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTa                                               >  2:619928/1‑128 (MQ=255)
                                                                            gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                              <  2:1202641/128‑1 (MQ=255)
                                                                                ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                           <  1:4227090/128‑1 (MQ=255)
                                                                                ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAATTCa                                          >  2:4321258/1‑128 (MQ=255)
                                                                                     aactaatctaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     <  2:2889884/128‑1 (MQ=255)
                                                                                     aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     >  1:3267733/1‑128 (MQ=255)
                                                                                     aactaaactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAACCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                     <  2:139209/128‑1 (MQ=255)
                                                                                      actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                    <  2:4817907/128‑1 (MQ=255)
                                                                                      actaaaataCACTCGATTATATAATAATATTTTTTTTTAATATAATAAGCAATTAAACACACATAAACTCACCCATTATGGTGATTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                    <  2:350751/128‑1 (MQ=255)
                                                                                       ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                   <  1:2157380/128‑1 (MQ=255)
                                                                                       ctaaactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                   <  2:4712795/128‑1 (MQ=255)
                                                                                          aactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                <  2:2785416/128‑1 (MQ=255)
                                                                                            ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                              <  1:317890/128‑1 (MQ=255)
                                                                                            ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                              >  1:4721815/1‑128 (MQ=255)
                                                                                            ataCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                              <  2:2485687/127‑1 (MQ=255)
                                                                                              acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                            >  2:327505/1‑128 (MQ=255)
                                                                                              acaatagATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACAAACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                            <  2:2584725/122‑1 (MQ=255)
                                                                                               cacaCGATAATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtggagc                            <  1:1612193/128‑6 (MQ=255)
                                                                                               cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                           >  2:1691520/1‑128 (MQ=255)
                                                                                                acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCATTTAAACACACAAAAACTACCCCATTATGGTGAGTTTAAAAATTATACAAAAATTTGATAATTTAAAAACAATTGAAGATCCacatt                          >  1:5311996/1‑124 (MQ=255)
                                                                                                acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                          >  2:52413/1‑128 (MQ=255)
                                                                                                 cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                         >  1:1521182/1‑128 (MQ=255)
                                                                                                 cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                         >  1:1426884/1‑128 (MQ=255)
                                                                                                     attaatTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCTTTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                     <  2:1117588/128‑1 (MQ=255)
                                                                                                        attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                  <  2:4011980/128‑1 (MQ=255)
                                                                                                         tttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAtt                 <  2:2035081/128‑1 (MQ=255)
                                                                                                         tttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAtt                 <  1:281585/128‑1 (MQ=255)
                                                                                                          ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                <  1:3007006/128‑1 (MQ=255)
                                                                                                           tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATCAATTTAATCTCCAGCTTCAACTAATTTa               >  2:927630/1‑128 (MQ=255)
                                                                                                           tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa               >  2:5523986/1‑128 (MQ=255)
                                                                                                           tAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATAAAGGTGAGTTTAAAAAATATTCAAAAATTTCATAATTTTAAATCAATTGATGCTCCAGCTTCAACTAATTTat              >  1:2903282/1‑127 (MQ=255)
                                                                                                            aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg              >  2:4971955/1‑128 (MQ=255)
                                                                                                            aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg              <  1:1111755/128‑1 (MQ=255)
                                                                                                              ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCTACTAATTTAGCt            >  1:2374908/1‑128 (MQ=255)
                                                                                                                atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGAttt          >  1:1884722/1‑128 (MQ=255)
                                                                                                                 atattttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTc         >  1:2048930/4‑128 (MQ=255)
                                                                                                                   tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAAACAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt       >  2:2286784/1‑128 (MQ=255)
                                                                                                                   tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt       >  1:4048208/1‑128 (MQ=255)
                                                                                                                    ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGTTTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt      <  2:4602238/128‑1 (MQ=255)
                                                                                                                     tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt     <  2:3921720/128‑1 (MQ=255)
                                                                                                                     tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt     <  2:4915404/128‑1 (MQ=255)
                                                                                                                      ttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt      >  1:3438348/1‑126 (MQ=255)
                                                                                                                              |                                                                                                                           
TATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTT  >  CP016816/484667‑484914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: